{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,22]],"date-time":"2026-08-22T02:05:50Z","timestamp":1787364350409,"version":"build-2736575974"},"reference-count":79,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T00:00:00Z","timestamp":1780358400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["32573272"],"award-info":[{"award-number":["32573272"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["U23A20234"],"award-info":[{"award-number":["U23A20234"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"award":["32573272"],"award-info":[{"award-number":["32573272"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"award":["U23A20234"],"award-info":[{"award-number":["U23A20234"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Natural Science Foundation of Xi\u2019an","award":["2025JH-ZRKX-0639"],"award-info":[{"award-number":["2025JH-ZRKX-0639"]}]},{"name":"Agricultural Innovation Technology Project and Key Agricultural Technology Core Research Project","award":["2025JCQY049"],"award-info":[{"award-number":["2025JCQY049"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>Modern dairy production has greatly increased milk yield, but high productivity is often accompanied by greater metabolic pressure, particularly during the transition period. Ketosis, fatty liver, and subacute ruminal acidosis are major disorders that limit health, efficiency, and sustainability in high-yielding dairy ruminants. This review examines the rumen microbiota as a central biological interface linking diet, ruminal fermentation, epithelial function, hepatic metabolism, and inflammation. Under homeostatic conditions, the rumen microbiota supports lactation by converting dietary fibre, starch, and nitrogen into volatile fatty acids, microbial protein, and other metabolites required for gluconeogenesis, milk component synthesis, and epithelial maintenance. However, under excessive nutritional or physiological stress, especially high-concentrate feeding and periparturient negative energy balance, this system may shift toward dysbiosis, acid accumulation, lipopolysaccharide release, epithelial barrier impairment, and activation of gut\u2013liver inflammatory pathways. These changes can contribute to the occurrence and interaction of subacute ruminal acidosis, ketosis, and fatty liver. We further summarize key factors affecting rumen microbial stability, including diet structure, host variation, physiological stage, environmental stress, feeding management, and ruminal epithelial volatile fatty acid absorption. Finally, microbiome-oriented strategies, such as gradual dietary transition, nutritional preconditioning, probiotics, postbiotics, functional metabolites, host metabolic support, and epithelial-targeted interventions, are discussed. Maintaining rumen microbial homeostasis should be regarded as a core principle for balancing high milk yield with long-term metabolic health. Future research should move beyond descriptive profiling toward causal validation of host\u2013microbe interactions and the development of microbiome-based early-warning and individualized nutritional management systems.<\/jats:p>","DOI":"10.3390\/vetsci13060546","type":"journal-article","created":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T00:50:28Z","timestamp":1780447828000},"page":"546","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Balancing High Yield and Metabolic Health in Dairy Ruminants: The Central Hub Role of the Rumen Microbiota"],"prefix":"10.3390","volume":"13","author":[{"given":"Xingwei","family":"Jiang","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology, Northwest A&F University, Yangling, Xianyang 712100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyi","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Northwest A&F University, Yangling, Xianyang 712100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiyang","family":"Sun","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Northwest A&F University, Yangling, Xianyang 712100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shixi","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Northwest A&F University, Yangling, Xianyang 712100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaodong","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Animal Science and Technology, Ningxia University, Yinchuan 750021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6251-8284","authenticated-orcid":false,"given":"Rongzhen","family":"Zhong","sequence":"additional","affiliation":[{"name":"Jilin Province Feed Processing and Ruminant Precision Breeding Cross Regional Cooperation Technology Innovation Center, Jilin Provincial Laboratory of Grassland Farming, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1033-2909","authenticated-orcid":false,"given":"Yangchun","family":"Cao","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Northwest A&F University, Yangling, Xianyang 712100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingyu","family":"Sun","sequence":"additional","affiliation":[{"name":"Jilin Province Feed Processing and Ruminant Precision Breeding Cross Regional Cooperation Technology Innovation Center, Jilin Provincial Laboratory of Grassland Farming, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1046-3477","authenticated-orcid":false,"given":"Shengru","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Northwest A&F University, Yangling, Xianyang 712100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1017\/S0962728600002220","article-title":"The impact of genetic selection for increased milk yield on the welfare of dairy cows","volume":"19","author":"Oltenacu","year":"2010","journal-title":"Anim. Welf."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"5684","DOI":"10.3168\/jds.2019-17271","article-title":"Symposium review: Nutrition strategies for improved health, production, and fertility during the transition period","volume":"103","author":"Cardoso","year":"2020","journal-title":"J. Dairy Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"3367","DOI":"10.3168\/jds.2023-23904","article-title":"Invited review: Ketone biology-The shifting paradigm of ketones and ketosis in the dairy cow","volume":"107","author":"Rico","year":"2024","journal-title":"J. Dairy Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"6945","DOI":"10.3168\/jds.2023-24626","article-title":"Global losses due to dairy cattle diseases: A comorbidity-adjusted economic analysis","volume":"107","author":"Rasmussen","year":"2024","journal-title":"J. Dairy Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1093\/tas\/txy102","article-title":"Prevalence of subclinical ketosis and production diseases in dairy cows in Central and South America, Africa, Asia, Australia, New Zealand, and Eastern Europe","volume":"3","author":"Brunner","year":"2019","journal-title":"Transl. Anim. Sci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"3105","DOI":"10.3168\/jds.S0022-0302(04)73446-3","article-title":"Invited Review: Pathology, Etiology, Prevention, and Treatment of Fatty Liver in Dairy Cows","volume":"87","author":"Bobe","year":"2004","journal-title":"J. Dairy Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"e1525","DOI":"10.1002\/vms3.1525","article-title":"Update on ketosis in dairy cattle with major emphasis on subclinical ketosis and abdominal adiposity","volume":"10","author":"Melendez","year":"2024","journal-title":"Vet. Med. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1016\/j.aninu.2021.12.008","article-title":"Subacute ruminal acidosis in dairy herds: Microbiological and nutritional causes, consequences, and prevention strategies","volume":"10","author":"Elmhadi","year":"2022","journal-title":"Anim. Nutr."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"7141","DOI":"10.3168\/jds.2022-21960","article-title":"Invited review: Effect of subacute ruminal acidosis on gut health of dairy cows","volume":"105","author":"Plaizier","year":"2022","journal-title":"J. Dairy Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/j.cvfa.2013.03.002","article-title":"Significance of metabolic stress, lipid mobilization, and inflammation on transition cow disorders","volume":"29","author":"Sordillo","year":"2013","journal-title":"Vet. Clin. N. Am. Food Anim. Pract."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/j.anireprosci.2013.11.007","article-title":"Interactions between negative energy balance, metabolic diseases, uterine health and immune response in transition dairy cows","volume":"144","author":"Esposito","year":"2014","journal-title":"Anim. Reprod. Sci."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Fu, Y., He, Y., Xiang, K., Zhao, C., He, Z., Qiu, M., Hu, X., and Zhang, N. (2022). The Role of Rumen Microbiota and Its Metabolites in Subacute Ruminal Acidosis (SARA)-Induced Inflammatory Diseases of Ruminants. Microorganisms, 10.","DOI":"10.3390\/microorganisms10081495"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1038\/s41579-021-00543-6","article-title":"The rumen microbiome: Balancing food security and environmental impacts","volume":"19","author":"Mizrahi","year":"2021","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.aninu.2022.04.002","article-title":"Understanding the role of rumen epithelial host-microbe interactions in cattle feed efficiency","volume":"10","author":"Na","year":"2022","journal-title":"Anim. Nutr."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1186\/s40168-022-01453-2","article-title":"The gastrointestinal microbiome in dairy cattle is constrained by the deterministic driver of the region and the modified effect of diet","volume":"11","author":"Lin","year":"2023","journal-title":"Microbiome"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"10094","DOI":"10.3168\/jds.2017-13320","article-title":"A 100-Year Review: Protein and amino acid nutrition in dairy cows","volume":"100","author":"Schwab","year":"2017","journal-title":"J. Dairy Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1038\/s41522-021-00215-6","article-title":"Real-time monitoring of ruminal microbiota reveals their roles in dairy goats during subacute ruminal acidosis","volume":"7","author":"Chen","year":"2021","journal-title":"npj Biofilms Microbiomes"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"e0149021","DOI":"10.1128\/msystems.01490-21","article-title":"Multi-omics Analysis Revealed Coordinated Responses of Rumen Microbiome and Epithelium to High-Grain-Induced Subacute Rumen Acidosis in Lactating Dairy Cows","volume":"7","author":"Mu","year":"2022","journal-title":"mSystems"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1016\/j.aninu.2021.10.009","article-title":"Responsive changes of rumen microbiome and metabolome in dairy cows with different susceptibility to subacute ruminal acidosis","volume":"8","author":"Zhang","year":"2022","journal-title":"Anim. Nutr."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1186\/s40168-025-02150-6","article-title":"Disruption of hindgut microbiome homeostasis promotes postpartum energy metabolism disorders in dairy ruminants by inhibiting acetate-mediated hepatic AMPK-PPARA axis","volume":"13","author":"Wang","year":"2025","journal-title":"Microbiome"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1186\/s40168-025-02072-3","article-title":"Rumen microbiome associates with postpartum ketosis development in dairy cows: A prospective nested case-control study","volume":"13","author":"Kong","year":"2025","journal-title":"Microbiome"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1186\/s40168-025-02202-x","article-title":"Altered ruminal microbiome tryptophan metabolism and their derived 3-indoleacetic acid inhibit ruminal inflammation in subacute ruminal acidosis goats","volume":"13","author":"Chen","year":"2025","journal-title":"Microbiome"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1186\/s13059-025-03789-y","article-title":"Multi-omics insights into microbiome-rumen epithelium interaction mechanisms underlying subacute rumen acidosis tolerance in dairy goats","volume":"26","author":"Xu","year":"2025","journal-title":"Genome Biol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"70142","DOI":"10.1002\/exp2.70142","article-title":"Rumen Mycobiome Thiamine Metabolism Contributes to Subacute Rumen Acidosis Tolerance in Goats Through Enhancing Epithelial Cell Proliferation via IGFBP2\/IGF1 Axis Activation","volume":"6","author":"Xu","year":"2026","journal-title":"Exploration"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"10066","DOI":"10.3168\/jds.2024-24817","article-title":"Invited review: Ruminal acidosis and its definition-A critical review","volume":"107","author":"Golder","year":"2024","journal-title":"J. Dairy Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"3969","DOI":"10.3168\/jds.2021-21115","article-title":"Subacute ruminal acidosis phenotypes in periparturient dairy cows differ in ruminal and salivary bacteria and in the in vitro fermentative activity of their ruminal microbiota","volume":"105","author":"Yang","year":"2022","journal-title":"J. Dairy Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"526","DOI":"10.1038\/s41598-025-30106-1","article-title":"Rumen metabolic environment of dairy cows varying in susceptibility of subacute ruminal acidosis modifies in vitro bacterial composition and fermentation","volume":"16","author":"Yang","year":"2025","journal-title":"Sci. Rep."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"856","DOI":"10.3168\/jds.S0022-0302(07)71569-2","article-title":"Ruminal lipopolysaccharide concentration and inflammatory response during grain-induced subacute ruminal acidosis in dairy cows","volume":"90","author":"Gozho","year":"2007","journal-title":"J. Dairy Sci."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1060","DOI":"10.3168\/jds.2008-1389","article-title":"A grain-based subacute ruminal acidosis challenge causes translocation of lipopolysaccharide and triggers inflammation","volume":"92","author":"Khafipour","year":"2009","journal-title":"J. Dairy Sci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.tvjl.2007.12.016","article-title":"Subacute ruminal acidosis in dairy cows: The physiological causes, incidence and consequences","volume":"176","author":"Plaizier","year":"2008","journal-title":"Vet. J."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1005","DOI":"10.3168\/jds.S0022-0302(97)76026-0","article-title":"Bovine acidosis: Implications on laminitis","volume":"80","author":"Nocek","year":"1997","journal-title":"J. Dairy Sci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"4967","DOI":"10.3168\/jds.2015-10354","article-title":"Nutritional strategies to optimize dairy cattle immunity","volume":"99","author":"Sordillo","year":"2016","journal-title":"J. Dairy Sci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1152\/physrev.1990.70.2.567","article-title":"Energy contributions of volatile fatty acids from the gastrointestinal tract in various species","volume":"70","author":"Bergman","year":"1990","journal-title":"Physiol. Rev."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3168\/jds.S0022-0302(77)83821-6","article-title":"Gluconeogenesis in cattle: Significance and methodology","volume":"60","author":"Young","year":"1977","journal-title":"J. Dairy Sci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"5883","DOI":"10.3168\/jds.2018-15955","article-title":"Invited review: Milk lactose\u2014Current status and future challenges in dairy cattle","volume":"102","author":"Costa","year":"2019","journal-title":"J. Dairy Sci."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1007\/s10911-021-09490-7","article-title":"A Comparative Review of the Cell Biology, Biochemistry, and Genetics of Lactose Synthesis","volume":"26","author":"Sadovnikova","year":"2021","journal-title":"J. Mammary Gland Biol. Neoplasia"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1146\/annurev.nutr.23.011702.073408","article-title":"Nutritional regulation of milk fat synthesis","volume":"23","author":"Bauman","year":"2003","journal-title":"Annu. Rev. Nutr."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"8857","DOI":"10.3168\/jds.2020-19622","article-title":"Rumen volatile fatty acid molar proportions, rumen epithelial gene expression, and blood metabolite concentration responses to ruminally degradable starch and fiber supplies","volume":"104","author":"Beckett","year":"2021","journal-title":"J. Dairy Sci."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"3620","DOI":"10.3168\/jds.S0022-0302(03)73968-X","article-title":"Rates of Production of Acetate, Propionate, and Butyrate in the Rumen of Lactating Dairy Cows Given Normal and Low-Roughage Diets","volume":"86","author":"Sutton","year":"2003","journal-title":"J. Dairy Sci."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1186\/s40104-021-00614-x","article-title":"Specific inhibition of Streptococcus bovis by endolysin LyJH307 supplementation shifts the rumen microbiota and metabolic pathways related to carbohydrate metabolism","volume":"12","author":"Kim","year":"2021","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Hackmann, T.J., and Firkins, J.L. (2015). Maximizing efficiency of rumen microbial protein production. Front. Microbiol., 6.","DOI":"10.3389\/fmicb.2015.00465"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"0682","DOI":"10.34133\/research.0682","article-title":"Alanine Derived from Ruminococcus_E bovis Alleviates Energy Metabolic Disorders during the Peripartum Period by Providing Glucogenic Precursors","volume":"8","author":"Kong","year":"2025","journal-title":"Research"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"7810","DOI":"10.3168\/jds.2023-24599","article-title":"Changes in the rumen microbial community composition of dairy cows subjected to an acidogenic diet","volume":"107","author":"Federiconi","year":"2024","journal-title":"J. Dairy Sci."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"E17","DOI":"10.3168\/jds.2006-478","article-title":"Ruminal acidosis in beef cattle: The current microbiological and nutritional outlook","volume":"90","author":"Nagaraja","year":"2007","journal-title":"J. Dairy Sci."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1186\/s40168-024-01964-0","article-title":"Ileal microbial microbiome and its secondary bile acids modulate susceptibility to nonalcoholic steatohepatitis in dairy goats","volume":"12","author":"Wang","year":"2024","journal-title":"Microbiome"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1002\/aro2.80","article-title":"Hepatic gluconeogenesis and regulatory mechanisms in lactating ruminants: A literature review","volume":"3","author":"Wang","year":"2025","journal-title":"Anim. Res. One Health"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1041","DOI":"10.3168\/jds.2011-4421","article-title":"Invited review: Role of physically effective fiber and estimation of dietary fiber adequacy in high-producing dairy cattle","volume":"95","author":"Zebeli","year":"2012","journal-title":"J. Dairy Sci."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1186\/s40168-019-0699-1","article-title":"Host genetics influence the rumen microbiota and heritable rumen microbial features associate with feed efficiency in cattle","volume":"7","author":"Li","year":"2019","journal-title":"Microbiome"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1186\/s40104-023-00862-z","article-title":"Multi-omics reveals that the host-microbiome metabolism crosstalk of differential rumen bacterial enterotypes can regulate the milk protein synthesis of dairy cows","volume":"14","author":"Zhang","year":"2023","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1186\/s42523-025-00443-7","article-title":"The bacterial faecal microbiota shifts during the transition period in dairy cows","volume":"7","author":"Arnalot","year":"2025","journal-title":"Anim. Microbiome"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"4014","DOI":"10.2527\/jas.2014-7621","article-title":"Temporal dynamics in the ruminal microbiome of dairy cows during the transition period","volume":"92","author":"Pitta","year":"2014","journal-title":"J. Anim. Sci."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1016\/j.rvsc.2021.04.029","article-title":"Dairy cow health and management in the transition period: The need to understand the human dimension","volume":"137","author":"Redfern","year":"2021","journal-title":"Res. Vet. Sci."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"104327","DOI":"10.1016\/j.jtherbio.2025.104327","article-title":"Alterations in the profiles of rumen microbiota and metabolites in Holstein and Jersey dairy cows under heat stress","volume":"134","author":"Li","year":"2025","journal-title":"J. Therm. Biol."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Cabral, L.D.S., and Weimer, P.J. (2024). Megasphaera elsdenii: Its Role in Ruminant Nutrition and Its Potential Industrial Application for Organic Acid Biosynthesis. Microorganisms, 12.","DOI":"10.3390\/microorganisms12010219"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"79","DOI":"10.4314\/sajas.v40i2.57275","article-title":"Ruminal acidosis: A review with detailed reference to the controlling agent Megasphaera elsdenii NCIMB 41125 (Review)","volume":"40","author":"Meissner","year":"2010","journal-title":"S. Afr. J. Anim. Sci."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Guo, J., Zhang, Z., Guan, L.L., Zhou, M., Yoon, I., Khafipour, E., and Plaizier, J.C. (2024). Postbiotics from Saccharomyces cerevisiae fermentation stabilize rumen solids microbiota and promote microbial network interactions and diversity of hub taxa during grain-based subacute ruminal acidosis (SARA) challenges in lactating dairy cows. Front. Microbiol., 15.","DOI":"10.3389\/fmicb.2024.1409659"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"7918","DOI":"10.3168\/jds.2024-24795","article-title":"Dietary supplementation of rumen native microbes improves lactation performance and feed efficiency in dairy cows","volume":"107","author":"Perdomo","year":"2024","journal-title":"J. Dairy Sci."},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Lobo, R.R., and Faciola, A.P. (2021). Ruminal Phages\u2014A Review. Front. Microbiol., 12.","DOI":"10.3389\/fmicb.2021.763416"},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Gilbert, R.A., Townsend, E.M., Crew, K.S., Hitch, T.C.A., Friedersdorff, J.C.A., Creevey, C.J., Pope, P.B., Ouwerkerk, D., and Jameson, E. (2020). Rumen Virus Populations: Technological Advances Enhancing Current Understanding. Front. Microbiol., 11.","DOI":"10.3389\/fmicb.2020.00450"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"100189","DOI":"10.1016\/j.engmic.2025.100189","article-title":"Applications of bacteriophages in precision engineering of the human gut microbiome","volume":"5","author":"Kuang","year":"2025","journal-title":"Eng. Microbiol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"607","DOI":"10.3168\/jds.2023-23441","article-title":"Effects of monensin supplementation on rumen fermentation, methane emissions, nitrogen balance, and metabolic responses of dairy cows: A systematic review and dose-response meta-analysis","volume":"107","author":"Norouzian","year":"2024","journal-title":"J. Dairy Sci."},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Luo, J., Jiang, J., Duan, H., Zhang, H., Sun, M., Mao, S., and Shen, J. (2024). Comparative effects of nisin and monensin supplementation on growth performance, rumen fermentation, nutrient digestion, and plasma metabolites of fattening Hu sheep. Front. Vet. Sci., 11.","DOI":"10.3389\/fvets.2024.1441431"},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Mammi, L.M.E., Guadagnini, M., Mechor, G., Cainzos, J.M., Fusaro, I., Palmonari, A., and Formigoni, A. (2021). The Use of Monensin for Ketosis Prevention in Dairy Cows during the Transition Period: A Systematic Review. Animals, 11.","DOI":"10.3390\/ani11071988"},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Sun, F., Cao, Y., Cai, C., Li, S., Yu, C., and Yao, J. (2016). Regulation of Nutritional Metabolism in Transition Dairy Cows: Energy Homeostasis and Health in Response to Post-Ruminal Choline and Methionine. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0160659"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1186\/s40104-022-00692-5","article-title":"Ruminal bacteria lipopolysaccharides: An immunological and microbial outlook","volume":"13","author":"Sarmikasoglou","year":"2022","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Caixeta, L.S., and Omontese, B.O. (2021). Monitoring and Improving the Metabolic Health of Dairy Cows during the Transition Period. Animals, 11.","DOI":"10.3390\/ani11020352"},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Souza, J.M., Ribeiro, P.H.C., and Millen, D.D. (2026). Review: Shifts of rumen microbiota by feeding non-fibrous carbohydrates to improve cattle performance. Front. Microbiol., 17.","DOI":"10.3389\/fmicb.2026.1735296"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.aninu.2020.10.005","article-title":"Influence of yeast on rumen fermentation, growth performance and quality of products in ruminants: A review","volume":"7","author":"Amin","year":"2021","journal-title":"Anim. Nutr."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1016\/j.cvfa.2017.06.009","article-title":"Rumen Microbiome, Probiotics, and Fermentation Additives","volume":"33","author":"McCann","year":"2017","journal-title":"Vet. Clin. N. Am. Food Anim. Pract."},{"key":"ref_70","doi-asserted-by":"crossref","unstructured":"Michalak, M., Wojnarowski, K., Cholewi\u0144ska, P., Szeligowska, N., Bawej, M., and Paco\u0144, J. (2021). Selected Alternative Feed Additives Used to Manipulate the Rumen Microbiome. Animals, 11.","DOI":"10.3390\/ani11061542"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"s78","DOI":"10.1017\/S1751731119003252","article-title":"Review: Ruminal microbiome and microbial metabolome: Effects of diet and ruminant host","volume":"14","author":"Newbold","year":"2020","journal-title":"Animal"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"1128","DOI":"10.1038\/s41396-023-01407-y","article-title":"Metabolic influence of core ciliates within the rumen microbiome","volume":"17","author":"Andersen","year":"2023","journal-title":"ISME J."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"236","DOI":"10.3168\/jdsc.2023-0433","article-title":"Rumen protozoa and viruses: The predators within and their functions\u2014A mini-review","volume":"5","author":"Yu","year":"2024","journal-title":"JDS Commun."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"e10","DOI":"10.1017\/anr.2024.10","article-title":"Rumen microbiome nutriomics: Harnessing omics technologies for enhanced understanding of rumen microbiome functions and ruminant nutrition","volume":"1","author":"Yu","year":"2024","journal-title":"Anim. Nutr."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1186\/s40168-020-00819-8","article-title":"Multi-omics reveals that the rumen microbiome and its metabolome together with the host metabolome contribute to individualized dairy cow performance","volume":"8","author":"Xue","year":"2020","journal-title":"Microbiome"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"e12","DOI":"10.1017\/anr.2024.17","article-title":"Culturomics: Bringing culture back to the forefront for effective gastrointestinal bacterial capture","volume":"1","author":"Chen","year":"2024","journal-title":"Anim. Nutr."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1186\/s40104-025-01182-0","article-title":"Functional analysis of Parabacteroides distasonis F4: A novel probiotic strain linked to calf growth and rumen fermentation","volume":"16","author":"Feng","year":"2025","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1186\/s40104-022-00738-8","article-title":"Susceptibility of dairy cows to subacute ruminal acidosis is reflected in both prepartum and postpartum bacteria as well as odd- and branched-chain fatty acids in feces","volume":"13","author":"Yang","year":"2022","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"skae041","DOI":"10.1093\/jas\/skae041","article-title":"Elucidating the factors and consequences of the severity of rumen acidosis in first-lactation Holstein cows during transition and early lactation","volume":"102","author":"Hartinger","year":"2024","journal-title":"J. Anim. Sci."}],"container-title":["Veterinary Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-7381\/13\/6\/546\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T04:52:32Z","timestamp":1780548752000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-7381\/13\/6\/546"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,2]]},"references-count":79,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2026,6]]}},"alternative-id":["vetsci13060546"],"URL":"https:\/\/doi.org\/10.3390\/vetsci13060546","relation":{},"ISSN":["2306-7381"],"issn-type":[{"value":"2306-7381","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,2]]}}}