{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T11:19:32Z","timestamp":1785410372001,"version":"3.56.0"},"reference-count":70,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T00:00:00Z","timestamp":1781740800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"CARS","award":["CARS-37"],"award-info":[{"award-number":["CARS-37"]}]},{"name":"Project of Seed Industry Revitalization in Jiangsu Province","award":["JBGS [2021]113"],"award-info":[{"award-number":["JBGS [2021]113"]}]},{"DOI":"10.13039\/501100008562","name":"Nanjing Agricultural University","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100008562","id-type":"DOI","asserted-by":"crossref"}]},{"id":[{"id":"https:\/\/ror.org\/05td3s095","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>Leucine, an essential branched-chain amino acid, serves not only as a substrate for protein synthesis but also as a key regulator of placental function and fetal development. This study investigated the effects of dietary supplementation with RP-Leu during late gestation on placental development and offspring performance in Hu sheep. Sixty twin-pregnant ewes at day 80 of pregnancy were randomly assigned to either a control group (fed a basal diet) or an RP-Leu group (fed a basal diet supplemented with 19 g\/day RP-Leu). The feeding trial lasted for 60 d. The ewes were slaughtered at day 140 of gestation. Maternal slaughter traits and fetal organ weights were recorded. Blood and milk samples were collected for milk composition analysis and targeted metabolomic profiling. Leucine supplementation significantly increased the percentage of milk fat content, total solid content, and the birth weight of lambs (p &lt; 0.05). Improvements in placental morphology and antioxidant capacity were observed, including a significant increase in cotyledon density and a significant enhancement of catalase (CAT) activity (p &lt; 0.05). Gene expression analysis indicated that the NOS3, SLC38A1 and FABP4 genes in the placental cotyledons (p &lt; 0.05), and the VEGFA, NOS3, SLC27A1 and FABP4 genes were significantly upregulated in the maternal caruncles (p &lt; 0.05). Plasma metabolomic profiling revealed increased L-glutamic acid levels and alterations in several amino acids, with pathway enrichment indicating involvement in amino acid metabolism and membrane transport processes. Transcriptomic analysis identified 739 differentially expressed genes, which were mainly enriched in the PI3K\/Akt signaling pathway, ECM\u2013receptor interaction pathway, and cytokine\u2013cytokine receptor interaction pathway. Collectively, these findings suggest that RP-Leu supplementation during late gestation may enhance offspring growth by modulating amino acid metabolism, promoting placental development, and improving placental nutrient transport capacity, thereby supporting fetal growth and development.<\/jats:p>","DOI":"10.3390\/vetsci13060592","type":"journal-article","created":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T10:25:34Z","timestamp":1781778334000},"page":"592","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Maternal Rumen-Protected Leucine Supplementation Enhances Placental Nutrient Transport Capacity and Increases Birth Weight in Hu Sheep"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-7767-4662","authenticated-orcid":false,"given":"Qin","family":"Gao","sequence":"first","affiliation":[{"name":"Jiangsu Livestock Embryo Engineering Laboratory, Nanjing Agricultural University, Nanjing 210095, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chong","family":"Yuan","sequence":"additional","affiliation":[{"name":"Jiangsu Livestock Embryo Engineering Laboratory, Nanjing Agricultural University, Nanjing 210095, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanglai","family":"Li","sequence":"additional","affiliation":[{"name":"Jiangsu Livestock Embryo Engineering Laboratory, Nanjing Agricultural University, Nanjing 210095, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua","family":"Yang","sequence":"additional","affiliation":[{"name":"Jiangsu Livestock Embryo Engineering Laboratory, Nanjing Agricultural University, Nanjing 210095, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zongyou","family":"Wei","sequence":"additional","affiliation":[{"name":"Taicang Animal Husbandry and Veterinary Station, Jiangsu Science and Technology Backyard, Enterprise Graduate Workstation, Taicang 215400, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7901-7774","authenticated-orcid":false,"given":"Yanli","family":"Zhang","sequence":"additional","affiliation":[{"name":"Jiangsu Livestock Embryo Engineering Laboratory, Nanjing Agricultural University, Nanjing 210095, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1007\/s00726-024-03417-2","article-title":"Branched-chain amino acids: Physico-chemical properties, industrial synthesis and role in signaling, metabolism and energy production","volume":"56","author":"Reifenberg","year":"2024","journal-title":"Amino Acids"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1016\/j.bbrc.2003.07.013","article-title":"Regulation of protein synthesis by branched-chain amino acids in vivo","volume":"313","author":"Yoshizawa","year":"2004","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"227s","DOI":"10.1093\/jn\/136.1.227S","article-title":"Signaling pathways and molecular mechanisms through which branched-chain amino acids mediate translational control of protein synthesis","volume":"136","author":"Kimball","year":"2006","journal-title":"J. Nutr."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1016\/S0006-291X(02)02717-1","article-title":"Leucine promotes glucose uptake in skeletal muscles of rats","volume":"299","author":"Nishitani","year":"2002","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1007\/s00726-015-2067-1","article-title":"The role of leucine and its metabolites in protein and energy metabolism","volume":"48","author":"Duan","year":"2016","journal-title":"Amino Acids"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.biocel.2017.05.026","article-title":"Leucine reduces reactive oxygen species levels via an energy metabolism switch by activation of the mTOR-HIF-1\u03b1 pathway in porcine intestinal epithelial cells","volume":"89","author":"Hu","year":"2017","journal-title":"Int. J. Biochem. Cell Biol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1017\/S0954422423000197","article-title":"The effects of branched-chain amino acids on muscle protein synthesis, muscle protein breakdown and associated molecular signalling responses in humans: An update","volume":"37","author":"Kaspy","year":"2024","journal-title":"Nutr. Res. Rev."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"115972","DOI":"10.1016\/j.anifeedsci.2024.115972","article-title":"Branched-chain amino acids in ruminant nutrition: Function effects and summary of recent advances","volume":"312","author":"An","year":"2024","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1071\/RD22234","article-title":"Maternal nutrition and developmental programming of offspring","volume":"35","author":"Reynolds","year":"2022","journal-title":"Reprod. Fertil. Dev."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"102305","DOI":"10.1016\/j.gde.2024.102305","article-title":"Fine-tuned programming of placenta trophoblast determines optimal maternal-fetal nutrient allocation","volume":"91","author":"Yu","year":"2025","journal-title":"Curr. Opin. Genet. Dev."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1113\/jphysiol.2006.104968","article-title":"Placental adaptive responses and fetal programming","volume":"572","author":"Myatt","year":"2006","journal-title":"J. Physiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2523","DOI":"10.1152\/physrev.00001.2023","article-title":"The placenta: Epigenetic insights into trophoblast developmental models of a generation-bridging organ with long-lasting impact on lifelong health","volume":"103","author":"Hemberger","year":"2023","journal-title":"Physiol. Rev."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1038358","DOI":"10.3389\/fcell.2022.1038358","article-title":"Genetic variation in placental insufficiency: What have we learned over time?","volume":"10","author":"Wang","year":"2022","journal-title":"Front. Cell Dev. Biol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.placenta.2021.11.004","article-title":"From stem cells to spiral arteries: A journey through early placental development","volume":"125","author":"James","year":"2022","journal-title":"Placenta"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Heras-Molina, A., Yeste, N., Pesantez-Pacheco, J.L., Astiz, S., Vazquez-Gomez, M., Bettiga, A., Trevisani, F., Garcia-Contreras, C., Luis-Lima, S., and Bassols, A. (2022). Obesity and Metabolic Traits after High-Fat Diet in Iberian Pigs with Low Birth Weight of Placental Origin. Biology, 11.","DOI":"10.3390\/biology11101533"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1113\/EP090442","article-title":"Placental adaptations supporting fetal growth during normal and adverse gestational environments","volume":"108","year":"2023","journal-title":"Exp. Physiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"43","DOI":"10.2478\/aoas-2021-0018","article-title":"Harnessing the Value of Rumen Protected Amino Acids to Enhance Animal Performance\u2014A Review","volume":"22","author":"Mazinani","year":"2022","journal-title":"Ann. Anim. Sci."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Huo, W., Lin, Y., Wang, C., and Deng, H. (2025). Methionine and its derivatives in dairy cow nutrition: Implications for intestinal barrier function, periparturient performance, and metabolic health. Front. Vet. Sci., 12.","DOI":"10.3389\/fvets.2025.1664853"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1007\/s00210-025-04472-6","article-title":"Regulation of PI3K\/Akt in preeclampsia: An examination of its pathological role and therapeutic potential","volume":"399","author":"Kamrani","year":"2026","journal-title":"Naunyn-Schmiedeberg\u2019s Arch. Pharmacol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"110060","DOI":"10.1016\/j.jnutbio.2025.110060","article-title":"Syncytin-1 deficiency impairs placental nutrient transport via PI3K\/Akt\/mTOR signaling","volume":"146","author":"Chen","year":"2025","journal-title":"J. Nutr. Biochem."},{"key":"ref_21","first-page":"14","article-title":"Brief communication: Maternally supplemented L-glutamine increases growth rates of twin lambs from birth","volume":"83","author":"Dunstana","year":"2023","journal-title":"New Zealand J. Anim. Sci. Prod."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Sa, R., Zhang, F., Yang, Z., Shi, C., Hou, S., Zhang, S., and Gui, L. (2025). Epigenetic landscape reveals MEF2 and SIX family-mediated transcriptional networks underlying dietary NFC\/NDF ratio-induced muscle development and meat quality in Tibetan sheep. Front. Nutr., 12.","DOI":"10.3389\/fnut.2025.1658319"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Birhan Anagaw, M., Mersha Chanie, K., Berihun, A.M., Muluken Yayeh, M., Yesuf, M., Tadegegn, M., Birhan, M., Ambaye, K., and Abraham Belete, T. (2025). Haematobiochemical alterations and lesion characterization induced by haemonchosis in slaughtered sheep at Gondar ELFORA abattoir, Ethiopia. BMC Vet. Res., 21.","DOI":"10.1186\/s12917-025-04478-5"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1111\/jpn.12069","article-title":"Leucine markedly regulates pancreatic exocrine secretion in goats","volume":"98","author":"Yu","year":"2014","journal-title":"J. Anim. Physiol. Anim. Nutr."},{"key":"ref_25","unstructured":"(2018). Laboratory Animal\u2014Guideline for Ethical Review of Animal Welfare (Standard No. GB\/T 35892-2018)."},{"key":"ref_26","unstructured":"(2021). Stability Test of Rumen Protected Amino Acids (Standard No. DB15\/T 2154\u20132021)."},{"key":"ref_27","unstructured":"(2016). National Food Safety Standard\u2014Determination of Amino Acids in Foods (Standard No. GB 5009.124)."},{"key":"ref_28","unstructured":"Zhang, L.Y.E. (2021). Feed Analysis and Quality Test Technology, China Agricultural University Press. [5th ed.]."},{"key":"ref_29","unstructured":"(2023). Technical Specification for the Performance Test of Goat and Sheep Stud (Standard No. NY\/T 1236-2023)."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/j.anireprosci.2013.07.010","article-title":"Maternal dietary intake alters organ mass and endocrine and metabolic profiles in pregnant ewe lambs","volume":"141","author":"Vonnahme","year":"2013","journal-title":"Anim. Reprod. Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.domaniend.2013.09.006","article-title":"Vonnahme Dietary selenium and nutritional plane alter specific aspects of maternal endocrine status during pregnancy and lactation","volume":"46","author":"Lemley","year":"2014","journal-title":"Domest. Anim. Endocrinol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"831","DOI":"10.1017\/S1751731114003243","article-title":"Colostrum production in ewes: A review of regulation mechanisms and of energy supply","volume":"9","author":"Banchero","year":"2015","journal-title":"Animal"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"560","DOI":"10.1631\/jzus.B1400337","article-title":"Leucine and histidine independently regulate milk protein synthesis in bovine mammary epithelial cells via mTOR signaling pathway","volume":"16","author":"Gao","year":"2015","journal-title":"J. Zhejiang Univ. Sci. B"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1007\/s11259-021-09799-y","article-title":"Implanting melatonin at lambing enhances lamb growth and maintains high fat content in milk","volume":"45","author":"Abecia","year":"2021","journal-title":"Vet. Res. Commun."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1477","DOI":"10.1007\/s00726-010-0612-5","article-title":"Supplementing L-leucine to a low-protein diet increases tissue protein synthesis in weanling pigs","volume":"39","author":"Yin","year":"2010","journal-title":"Amino Acids"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"109732","DOI":"10.1016\/j.jnutbio.2024.109732","article-title":"L-leucine promotes the synthesis of milk protein and milk fat in bovine mammary epithelial cells through the AKT\/mTOR signaling pathway under hypoxic conditions","volume":"134","author":"Liu","year":"2024","journal-title":"J. Nutr. Biochem."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"8884","DOI":"10.1021\/acs.jafc.9b03574","article-title":"Leucine Promotes Milk Synthesis in Bovine Mammary Epithelial Cells via the PI3K-DDX59 Signaling","volume":"67","author":"Qiu","year":"2019","journal-title":"J. Agric. Food Chem."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"2873","DOI":"10.1007\/s00394-023-03197-7","article-title":"Leucine and arginine enhance milk fat and milk protein synthesis via the CaSR\/\/mTORC1 and CaSR\/\/mTORC1 pathways","volume":"62","author":"Li","year":"2023","journal-title":"Eur. J. Nutr."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1496","DOI":"10.1038\/s12276-020-00504-8","article-title":"Glutamine reliance in cell metabolism","volume":"52","author":"Yoo","year":"2020","journal-title":"Exp. Mol. Med."},{"key":"ref_40","first-page":"1","article-title":"Biosynthesis of food constituents: Amino acids: 1. The glutamic acid and aspartic acid groups\u2014A review","volume":"24","author":"Cejpek","year":"2006","journal-title":"Czech J. Food Sci."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"18430","DOI":"10.1038\/s41598-021-97855-7","article-title":"Persistent hyperammonia and altered concentrations of urea cycle metabolites in a 5-day swine experiment of sepsis","volume":"11","author":"Ferrario","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"14606","DOI":"10.3390\/ijms131114606","article-title":"Roles of nitric oxide and asymmetric dimethylarginine in pregnancy and fetal programming","volume":"13","author":"Huang","year":"2012","journal-title":"Int. J. Mol. Sci."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"7984","DOI":"10.1002\/jcp.30470","article-title":"Novel insights for the role of nitric oxide in placental vascular function during and beyond pregnancy","volume":"236","author":"Krause","year":"2021","journal-title":"J. Cell. Physiol."},{"key":"ref_44","first-page":"E13","article-title":"Production and utilization of amino acids by ovine placenta in vivo","volume":"274","author":"Chung","year":"1998","journal-title":"Am. J. Physiol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"2518s","DOI":"10.1093\/jn\/nxaa155","article-title":"Methionine Nutrition and Metabolism: Insights from Animal Studies to Inform Human Nutrition","volume":"150","author":"Elango","year":"2020","journal-title":"J. Nutr."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/j.copbio.2019.10.001","article-title":"Quantitative analysis of glycine related metabolic pathways for one-carbon synthetic biology","volume":"64","author":"Hong","year":"2020","journal-title":"Curr. Opin. Biotechnol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"3152","DOI":"10.1111\/j.1742-4658.2011.08241.x","article-title":"Glutamine and \u03b1-ketoglutarate as glutamate sources for glutathione synthesis in human erythrocytes","volume":"278","author":"Whillier","year":"2011","journal-title":"FEBS J."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1093\/advances\/nmz015","article-title":"Perspective: L-arginine and L-citrulline Supplementation in Pregnancy: A Potential Strategy to Improve Birth Outcomes in Low-Resource Settings","volume":"10","author":"Weckman","year":"2019","journal-title":"Adv. Nutr."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"123","DOI":"10.4314\/sajas.v31i2.3838","article-title":"Environmental and genetic aspects of survival and early liveweight in Western Australian Merino sheep","volume":"31","author":"Cloete","year":"2001","journal-title":"S. Afr. J. Anim. Sci."},{"key":"ref_50","first-page":"112","article-title":"The effect of age of ewes and lambing season on litter size and weight of lambs","volume":"19","year":"2002","journal-title":"Vet. Ir Zootech."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1016\/j.smallrumres.2005.03.003","article-title":"Reproductive performance and mortality rate in Menz and Horro sheep following controlled breeding in Ethiopia","volume":"63","author":"Berhan","year":"2006","journal-title":"Small Rumin. Res."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"213","DOI":"10.2298\/BAH0904213P","article-title":"Effect of environmental and paragenetic factors on birth mass variability of Mis sheep populations","volume":"25","year":"2009","journal-title":"Biotechnol. Anim. Husb."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"459","DOI":"10.5713\/ajas.2009.80318","article-title":"Genetic and Non-genetic Factors Affecting Mortality in Lori-Bakhtiari Lambs","volume":"22","author":"Vatankhah","year":"2009","journal-title":"Asian-Australas. J. Anim. Sci."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1758","DOI":"10.2527\/jas.2007-0132","article-title":"Genetic parameters for early lamb survival and growth in Scottish Blackface sheep","volume":"86","author":"Riggio","year":"2008","journal-title":"J. Anim. Sci."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1185","DOI":"10.1007\/s00726-011-0983-2","article-title":"Leucine nutrition in animals and humans: mTOR signaling and beyond","volume":"41","author":"Li","year":"2011","journal-title":"Amino Acids"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"E854","DOI":"10.1152\/ajpendo.00153.2003","article-title":"Potential role of leucine metabolism in the leucine-signaling pathway involving mTOR","volume":"285","author":"Lynch","year":"2003","journal-title":"Am. J. Physiol.-Endocrinol. Metab."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1016\/S0002-9378(99)70230-9","article-title":"Fetal supply of amino acids and amino nitrogen after maternal infusion of amino acids in pregnant sheep","volume":"180","author":"Teng","year":"1999","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1088","DOI":"10.1016\/j.ajog.2005.12.056","article-title":"Human fetal growth and organ development: 50 years of discoveries","volume":"194","author":"Pardi","year":"2006","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"3751","DOI":"10.1046\/j.1432-1033.2002.03074.x","article-title":"Control of p70 ribosomal protein S6 kinase and acetyl-CoA carboxylase by AMP-activated protein kinase and protein phosphatases in isolated hepatocytes","volume":"269","author":"Krause","year":"2002","journal-title":"Eur. J. Biochem."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"3742","DOI":"10.1046\/j.1432-1033.2002.03069.x","article-title":"Signalling pathways and combinatory effects of insulin and amino acids in isolated rat hepatocytes","volume":"269","author":"Krause","year":"2002","journal-title":"Eur. J. Biochem."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"364976","DOI":"10.1155\/2014\/364976","article-title":"Metabolic and Physiological Roles of Branched-Chain Amino Acids","volume":"2014","author":"Monirujjaman","year":"2014","journal-title":"Adv. Mol. Biol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"2320","DOI":"10.1074\/jbc.270.5.2320","article-title":"Phosphorylation of Ribosomal-Protein S6 Is Inhibitory for Autophagy in Isolated Rat Hepatocytes","volume":"270","author":"Blommaart","year":"1995","journal-title":"J. Biol. Chem."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"824","DOI":"10.1016\/0026-0495(88)90115-1","article-title":"Effect of Total Parenteral-Nutrition Enriched in Branched-Chain Amino-Acids on Metabolite Levels in Septic Rats","volume":"37","author":"Mori","year":"1988","journal-title":"Metab.-Clin. Exp."},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Gao, X.J., Tian, F., Wang, X.Y., Zhao, J., Wan, X., Zhang, L., Wu, C., Li, N., and Li, J.S. (2015). Leucine Supplementation Improves Acquired Growth Hormone Resistance in Rats with Protein-Energy Malnutrition. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0125023"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"293271","DOI":"10.1155\/2015\/293271","article-title":"Oxidative Stress in Placenta: Health and Diseases","volume":"2015","author":"Wu","year":"2015","journal-title":"BioMed Res. Int."},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Shi, Y., Wang, C.-C., Wu, L., Zhang, Y., Xu, A., and Wang, Y. (2023). Pathophysiological Insight into Fatty Acid-Binding Protein-4: Multifaced Roles in Reproduction, Pregnancy, and Offspring Health. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms241612655"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1111\/j.1476-5381.2010.01196.x","article-title":"Therapeutic effect of enhancing endothelial nitric oxide synthase (eNOS) expression and preventing eNOS uncoupling","volume":"164","author":"Li","year":"2011","journal-title":"Br. J. Pharmacol."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1111\/micc.12093","article-title":"Regulation of Placental Angiogenesis","volume":"21","author":"Chen","year":"2014","journal-title":"Microcirculation"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"dev175596","DOI":"10.1242\/dev.175596","article-title":"The extracellular matrix in development","volume":"147","author":"Walma","year":"2020","journal-title":"Development"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1146\/annurev.cellbio.22.010305.104315","article-title":"Of extracellular matrix, scaffolds, and signaling: Tissue architecture regulates development, homeostasis, and cancer","volume":"22","author":"Nelson","year":"2006","journal-title":"Annu. Rev. Cell Dev. Biol."}],"container-title":["Veterinary Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-7381\/13\/6\/592\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T04:24:30Z","timestamp":1781929470000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-7381\/13\/6\/592"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,18]]},"references-count":70,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2026,6]]}},"alternative-id":["vetsci13060592"],"URL":"https:\/\/doi.org\/10.3390\/vetsci13060592","relation":{},"ISSN":["2306-7381"],"issn-type":[{"value":"2306-7381","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,18]]}}}