{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T04:45:36Z","timestamp":1782794736318,"version":"3.54.5"},"reference-count":35,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T00:00:00Z","timestamp":1782432000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Open Project of Key Laboratory of Innovative Utilization of Unconventional Feed Resources","award":["FCG-2023-08"],"award-info":[{"award-number":["FCG-2023-08"]}]},{"name":"Cultivating Young Backbone Teachers in Higher Education Institutions","award":["2024GGJS152"],"award-info":[{"award-number":["2024GGJS152"]}]},{"name":"Key Project of International Science and Technology Cooperation","award":["251111520200"],"award-info":[{"award-number":["251111520200"]}]},{"name":"Science and Technology Tackling Key Problems Project","award":["252102110070"],"award-info":[{"award-number":["252102110070"]}]},{"name":"Science and Technology Tackling Key Problems Project","award":["232102111047"],"award-info":[{"award-number":["232102111047"]}]},{"name":"Doctoral Research Start-up Fund Project of Henan University of Economics and Animal Husbandry","award":["2023HNUAHEDF007"],"award-info":[{"award-number":["2023HNUAHEDF007"]}]},{"name":"Key Scientific Research Project of Higher Education Institutions","award":["25A230008"],"award-info":[{"award-number":["25A230008"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>Aflatoxin B1 (AFB1) causes acute liver injury in livestock. This study evaluated whether Vitamin U could alleviate AFB1-induced hepatotoxicity in mice. AFB1 (3 mg\/kg) reduced PLT, PCT, and EOS counts, caused hepatic vascular congestion, and decreased GSH-Px activity. Vitamin U (50 mg\/kg) significantly improved these hematological parameters, alleviated central venous and sinusoidal congestion, increased T-SOD activity and upregulated IL-10 mRNA expression. However, Vitamin U did not significantly reverse AFB1-induced elevation of ALP or reduction in GSH-Px, nor did it affect ALT, AST, or protein levels of the Nrf2\/Keap1 pathway. Transcriptomic analysis revealed enrichment of DEGs in immune- and cell cycle-related pathways, with no direct enrichment observed in the Nrf2\/Keap1 pathway. Vitamin U upregulated Keap1 mRNA expression but did not alter KEAP1 protein levels. In conclusion, Vitamin U partially protects against AFB1-induced acute liver injury by ameliorating thrombocytopenia, vascular congestion, enhancing T-SOD activity, and upregulating IL-10 expression, providing preliminary experimental evidence for further investigation.<\/jats:p>","DOI":"10.3390\/vetsci13070621","type":"journal-article","created":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T02:11:57Z","timestamp":1782785517000},"page":"621","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Vitamin U Attenuates Acute Aflatoxin B1-Induced Liver Injury in Mice: Biochemical, Histological and Transcriptomic Evidence"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6710-585X","authenticated-orcid":false,"given":"Liyu","family":"Yang","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, China"},{"name":"Henan Pig Bio-Breeding Research Institute, Zhengzhou 450046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaxin","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuanxuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yake","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shufan","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenxi","family":"Ling","sequence":"additional","affiliation":[{"name":"College of Animal Sciences, Xinjiang Agricultural University, Wulumuqi 830052, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinfeng","family":"Li","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-8053-5591","authenticated-orcid":false,"given":"Kun","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, China"},{"name":"Henan Key Laboratory of Livestock and Poultry Genetic Improvement and Healthy Breeding, Zhengzhou 450046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Huo","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangwei","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, China"},{"name":"Henan Pig Bio-Breeding Research Institute, Zhengzhou 450046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiuliang","family":"Xu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, China"},{"name":"Henan Pig Bio-Breeding Research Institute, Zhengzhou 450046, China"},{"name":"Henan Livestock and Poultry Genetic Resources Protection Engineering Technology Research Center, Zhengzhou 450046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongyu","family":"Deng","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, China"},{"name":"Henan Key Laboratory of Livestock and Poultry Genetic Improvement and Healthy Breeding, Zhengzhou 450046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0914-8955","authenticated-orcid":false,"given":"Congcong","family":"Li","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, China"},{"name":"Henan Pig Bio-Breeding Research 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