{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T05:16:23Z","timestamp":1783574183999,"version":"3.55.0"},"reference-count":38,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T00:00:00Z","timestamp":1783382400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Open Project Program of Fujian Provincial Key Laboratory for Prevention and Control of Animal Infectious Diseases and Biotechnology","award":["ZDSYS2024001"],"award-info":[{"award-number":["ZDSYS2024001"]}]},{"DOI":"10.13039\/501100012246","name":"Priority Academic Program Development of Jiangsu Higher Education Institutions","doi-asserted-by":"crossref","award":["D18007"],"award-info":[{"award-number":["D18007"]}],"id":[{"id":"10.13039\/501100012246","id-type":"DOI","asserted-by":"crossref"}]},{"name":"the Fourth Batch of the Talent Project in Fujian Province","award":["110"],"award-info":[{"award-number":["110"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>Feline and canine coronaviruses (FCoVs and CCoVs) are widely prevalent in companion animals. The recent emergence of a highly virulent FCoV-CCoV recombinant virus, FCoV-23, caused a rapid feline infectious peritonitis (FIP) outbreak in Cyprus, which raises a serious concern about the cross-species transmission of CoVs among companion animals. Here, we evaluated the prevalence, transmission and evolution of FCoV and CCoV in the Jiangsu and Zhejiang provinces of China in 2025. A universal RT-qPCR assay for FCoV and CCoV was established and used for the detection of 700 clinical samples, including 501 from cats and 199 from dogs. A total of 92 samples (13.14%, 92\/700) were detected as positive, containing 79 positive samples (15.76%, 79\/501) from cats and 13 positive samples (6.5%, 13\/199) from dogs. Ten complete S genes and two complete genomes were obtained from positive samples. Fragment comparison and phylogenetic analysis identified one CCoV-I sequence from a feline sample and one FCoV-II sequence from a canine sample. Furthermore, recombination analyses not only detected intra-species recombination events but also inter-species cross-over events. Collectively, this study, for the first time, revealed that cross-species transmission and recombination events occurred between FCoV and CCoV among companion animals in the Jiangsu\u2013Zhejiang region of China in 2025.<\/jats:p>","DOI":"10.3390\/vetsci13070661","type":"journal-article","created":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T15:00:32Z","timestamp":1783436432000},"page":"661","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Cross-Species Transmission and Recombination Between Feline and Canine Coronaviruses in Jiangsu\u2013Zhejiang Region in 2025"],"prefix":"10.3390","volume":"13","author":[{"given":"Yanhan","family":"Lin","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China"},{"name":"Longyan University and Fujian Provincial Key Laboratory for Prevention and Control of Animal Infectious Diseases and Biotechnology, Longyan 364012, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyang","family":"Zhu","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yifan","family":"Meng","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiachun","family":"Zou","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanying","family":"Xie","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuai","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng","family":"Cui","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Qiu","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinkai","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinchao","family":"Guan","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Lin","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China"},{"name":"Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, China"},{"name":"Comparative Medicine Research Institute, Yangzhou University, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sen","family":"Jiang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China"},{"name":"Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, China"},{"name":"Comparative Medicine Research Institute, Yangzhou University, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0939-331X","authenticated-orcid":false,"given":"Wanglong","family":"Zheng","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China"},{"name":"Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, China"},{"name":"Comparative Medicine Research Institute, Yangzhou University, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7082-1993","authenticated-orcid":false,"given":"Jianzhong","family":"Zhu","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China"},{"name":"Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, China"},{"name":"Comparative Medicine Research Institute, Yangzhou University, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kewei","family":"Fan","sequence":"additional","affiliation":[{"name":"Longyan University and Fujian Provincial Key Laboratory for Prevention and Control of Animal Infectious Diseases and Biotechnology, Longyan 364012, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1909-8760","authenticated-orcid":false,"given":"Nanhua","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China"},{"name":"Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, China"},{"name":"Comparative Medicine Research Institute, Yangzhou University, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1038\/s41586-025-09007-w","article-title":"A MERS-CoV-like mink coronavirus uses ACE2 as an entry receptor","volume":"642","author":"Wang","year":"2025","journal-title":"Nature"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/S0166-0934(03)00081-8","article-title":"Genetic diversity of a canine coronavirus detected in pups with diarrhoea in Italy","volume":"110","author":"Pratelli","year":"2003","journal-title":"J. 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