{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T11:24:40Z","timestamp":1783596280796,"version":"3.55.0"},"reference-count":27,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T00:00:00Z","timestamp":1783555200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>Extraintestinal pathogenic Escherichia coli (ExPEC) are opportunistic pathogens in pigs and can cause systemic infections with neurological signs. Cases in suckling piglets have rarely been reported. In November 2024, suckling piglets aged 1\u20133 weeks on a commercial pig farm developed neurological signs, including altered consciousness, vertical nystagmus, ataxic gait, and recumbency. Within two affected batches comprising 1150 and 1200 piglets, 17 and 26 animals, respectively, developed clinical signs (morbidity 1.48% and 2.17%). Initial treatment with amoxicillin and dexamethasone was ineffective. Early administration of intramuscular enrofloxacin combined with dexamethasone was associated with clinical improvement in approximately 50% of affected piglets. Pathological examination revealed atelectatic lung regions, dilated small intestines with yellowish content, and moderate mesenteric vessel congestion. Histologically, the central nervous system of both piglets exhibited hyperemia, edema, disseminated diapedesis hemorrhages, thrombi, and intravascular rod-shaped bacteria. E. coli was isolated from the CNS, lungs, pericardium, and joints. Molecular analysis confirmed the presence of virulence-associated genes fimA, fimH, and iucD. Differential diagnostic PCRs for Streptococcus suis, Glaesserella parasuis, and Mycoplasma hyorhinis were negative. This case report documents the first occurrence of an ExPEC-like E. coli infection in this herd. The documented clinical, pathological, and molecular findings support the importance of considering ExPEC-like E. coli as a differential diagnosis in therapy-resistant neurological diseases in piglets.<\/jats:p>","DOI":"10.3390\/vetsci13070666","type":"journal-article","created":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T10:16:22Z","timestamp":1783592182000},"page":"666","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Extraintestinal Pathogenic Escherichia coli (ExPEC)-like Infection Associated with Septicemia and Neurological Signs in Suckling Piglets: A Case Report"],"prefix":"10.3390","volume":"13","author":[{"given":"Annika","family":"Gersemann","sequence":"first","affiliation":[{"name":"Veterinary Practice Dr. Bernd Kiene & Kollegen GmbH, Am H\u00f6vel 12, 49439 Steinfeld-M\u00fchlen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4284-0345","authenticated-orcid":false,"given":"Gerald","family":"Reiner","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Sciences, Clinic for Swine, Justus Liebig University Giessen, Frankfurter Strasse 112, 35392 Giessen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonja von","family":"Berg","sequence":"additional","affiliation":[{"name":"Veterinary Practice Dr. Bernd Kiene & Kollegen GmbH, Am H\u00f6vel 12, 49439 Steinfeld-M\u00fchlen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e00135-18","DOI":"10.1128\/CMR.00135-18","article-title":"Global Extraintestinal Pathogenic Escherichia coli (ExPEC) Lineages","volume":"32","author":"Manges","year":"2019","journal-title":"Clin. Microbiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1093\/infdis\/jiv429","article-title":"Extraintestinal Pathogenic Escherichia coli, a Common Human Pathogen: Challenges for Vaccine Development and Progress in the Field","volume":"213","author":"Poolman","year":"2016","journal-title":"J. Infect. Dis."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"e1236","DOI":"10.1093\/cid\/ciac421","article-title":"Global Distribution of O Serotypes and Antibiotic Resistance in Extraintestinal Pathogenic Escherichia coli Collected From the Blood of Patients With Bacteremia Across Multiple Surveillance Studies","volume":"76","author":"Weerdenburg","year":"2023","journal-title":"Clin. Infect. Dis."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"RP91853","DOI":"10.7554\/eLife.91853.3","article-title":"High-risk Escherichia coli clones that cause neonatal meningitis and association with recrudescent infection","volume":"12","author":"Nhu","year":"2024","journal-title":"elife"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"104265","DOI":"10.1016\/j.meegid.2020.104265","article-title":"The evolutionary puzzle of Escherichia coli ST131","volume":"81","author":"Pitout","year":"2020","journal-title":"Infect. Genet Evol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1128\/microbiolspec.UTI-0006-2012","article-title":"Reservoirs of Extraintestinal Pathogenic Escherichia coli","volume":"3","author":"Manges","year":"2015","journal-title":"Microbiol. Spectr."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Guragain, M., Bagi, L., Liu, Y., and Bosilevac, J.M. (2025). Characterization of Extraintestinal Pathogenic Escherichia coli from Human Clinical and Poultry Samples. Microorganisms, 13.","DOI":"10.3390\/microorganisms13112603"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"5811","DOI":"10.1038\/s41467-024-50268-2","article-title":"Population structure and antibiotic resistance of swine extraintestinal pathogenic Escherichia coli from China","volume":"15","author":"Li","year":"2024","journal-title":"Nat. Commun."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2005","DOI":"10.1111\/tbed.13848","article-title":"Screening virulence factors of porcine extraintestinal pathogenic Escherichia coli (an emerging pathotype) required for optimal growth in swine blood","volume":"68","author":"Ma","year":"2021","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_10","first-page":"321","article-title":"Molecular characterization of extraintestinal pathogenic Escherichia coli (ExPEC) pathogenicity islands in F165-positive E. coli strain from a diseased animal","volume":"238","author":"Dezfulian","year":"2004","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1742","DOI":"10.1111\/j.1462-2920.2008.01595.x","article-title":"ExPEC-typical virulence-associated genes correlate with successful colonization by intestinal E. coli in a small piglet group","volume":"10","author":"Schierack","year":"2008","journal-title":"Environ. Microbiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"5038","DOI":"10.1128\/JB.05551-11","article-title":"Genome sequence of a porcine extraintestinal pathogenic Escherichia coli strain","volume":"193","author":"Tan","year":"2011","journal-title":"J. Bacteriol."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Zhu, Y., Dong, W., Ma, J., Yuan, L., Hejair, H.M., Pan, Z., Liu, G., and Yao, H. (2017). Characterization and virulence clustering analysis of extraintestinal pathogenic Escherichia coli isolated from swine in China. BMC Vet. Res., 13.","DOI":"10.1186\/s12917-017-0975-x"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1016\/j.tvjl.2011.06.038","article-title":"Serotypes and virulence genes of extraintestinal pathogenic Escherichia coli isolates from diseased pigs in China","volume":"192","author":"Tan","year":"2012","journal-title":"Vet. J."},{"key":"ref_15","first-page":"421","article-title":"Polyserositis in pigs due to generalized Escherichia coli infection","volume":"39","author":"Nielsen","year":"1975","journal-title":"Can. J. Comp. Med."},{"key":"ref_16","first-page":"119","article-title":"Durch Extraintestinal pathogene Escherichia coli (ExPEC) bedingte haemorrhagische Septikaemie beim Schwein als Differentialdiagnose zur Klassischen Schweinepest-Fallbericht und Literatur\u00fcbersicht [Haemorrhagic septicaemia in a pig caused by extraintestinal pathogenic Escherichia coli (ExPEC) as a differential diagnosis in classical swine fever-case report and review of the literature]","volume":"123","author":"Reiner","year":"2010","journal-title":"Berl. Munch. Tierarztl. Wochenschr."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"747","DOI":"10.12935\/jvma.67.747","article-title":"2014 Fibrinopurulent Meningitis Caused by Extraintestinal Pathogenic Escherichia coli O166 in Suckling Piglets","volume":"67","author":"Kizaki","year":"2014","journal-title":"J. Jpn. Vet. Med. Assoc."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Kong, L.C., Guo, X., Wang, Z., Gao, Y.H., Jiu, S.M., and Ma, H.X. (2017). Whole genome sequencing of an ExPEC that caused fatal pneumonia at a pig farm in Changchun, China. BMC Vet. Res., 13.","DOI":"10.1186\/s12917-017-1093-5"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"642","DOI":"10.1016\/j.ijmm.2011.09.006","article-title":"What defines extraintestinal pathogenic Escherichia coli?","volume":"301","author":"Kohler","year":"2011","journal-title":"Int. J. Med. Microbiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"546","DOI":"10.1128\/IAI.67.2.546-553.1999","article-title":"The link between phylogeny and virulence in Escherichia coli extraintestinal infection","volume":"67","author":"Picard","year":"1999","journal-title":"Infect. Immun."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1186\/1751-0147-51-26","article-title":"Bacteria in milk from anterior and posterior mammary glands in sows affected and unaffected by postpartum dysgalactia syndrome (PPDS)","volume":"51","author":"Kemper","year":"2009","journal-title":"Acta Vet. Scand."},{"key":"ref_22","first-page":"394","article-title":"Bacterial diversity in sow milk from healthy sows and sows with postpartum dysgalactia syndrome","volume":"179","author":"Angjelovski","year":"2016","journal-title":"Vet. Rec."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Zimmerman, J.J., Karriker, L.A., Ramirez, A., Schwartz, K.J., Stevenson, G.W., and Zhang, J. (2019). Colibacillosis. Diseases of Swine, Wiley Blackwell. [11th ed.].","DOI":"10.1002\/9781119350927"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Zimmerman, J.J., Karriker, L.A., Ramirez, A., Schwartz, K.J., Stevenson, G.W., and Zhang, J. (2019). Gl\u00e4sser\u2019s Disease. Diseases of Swine, Wiley Blackwell. [11th ed.].","DOI":"10.1002\/9781119350927"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1186\/s40813-020-00178-8","article-title":"Mycoplasma hyorhinis as a possible cause of fibrinopurulent meningitis in pigs?\u2014A case series","volume":"6","author":"Brunthaler","year":"2020","journal-title":"Porc. Health Manag."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Zimmerman, J.J., Karriker, L.A., Ramirez, A., Schwartz, K.J., Stevenson, G.W., and Zhang, J. (2019). Streptococcosis. Diseases of Swine, Wiley Blackwell. [11th ed.].","DOI":"10.1002\/9781119350927"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Zimmerman, J.J., Karriker, L.A., Ramirez, A., Schwartz, K.J., Stevenson, G.W., and Zhang, J. (2019). Mycoplasmosis. Diseases of Swine, Wiley Blackwell. 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