{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T15:30:00Z","timestamp":1785771000888,"version":"3.56.0"},"reference-count":40,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,8,2]],"date-time":"2026-08-02T00:00:00Z","timestamp":1785628800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Key Research and Development Program Project","award":["2024YFD1800304"],"award-info":[{"award-number":["2024YFD1800304"]}]},{"name":"Yunnan Provincial Science and Technology Talent and Platform Program","award":["202505AF350101"],"award-info":[{"award-number":["202505AF350101"]}]},{"DOI":"10.13039\/501100018927","name":"Southwest Minzu University Research Startup Funds","doi-asserted-by":"publisher","award":["RQD2021001"],"award-info":[{"award-number":["RQD2021001"]}],"id":[{"id":"10.13039\/501100018927","id-type":"DOI","asserted-by":"publisher"}]},{"award":["RQD2021001"],"award-info":[{"award-number":["RQD2021001"]}],"id":[{"id":"https:\/\/ror.org\/04gaexw88","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vet. Sci.","Veterinary Sciences"],"abstract":"<jats:p>Timely detection of specific antibodies against foot-and-mouth disease virus (FMDV) is critical to effective prevention and control of the disease. In this study, we developed a competitive chemiluminescent enzyme immunoassay (cCLEIA) based on recombinant FMDV VP2 protein (FMDV-VP2 cCLEIA) for simultaneous detection of antibodies against FMDV serotypes O and A in serum samples. Using a cut-off of 35% percentage inhibition (PI), the FMDV-VP2 cCLEIA achieved a relative sensitivity of 101.5% and a relative specificity of 102% compared with commercial FMDV serotypes O and A liquid-phase blocking ELISA (LPBE) kits. The assay showed high specificity for detection of antibodies against FMDV serotypes A and O, with no cross-reactivity observed against positive sera for classical swine fever virus, porcine reproductive and respiratory syndrome virus, and Senecavirus A. Both intra-batch and inter-batch coefficients of variation were below 15%, confirming excellent assay reproducibility. The testing of 40 field serum samples yielded a positive detection rate of 95% using the FMDV-VP2 cCLEIA, compared with 92.5% using the FMDV serotypes O and A LPBE kits. In conclusion, the FMDV-VP2 cCLEIA developed is a rapid, sensitive assay for simultaneous detection of antibodies against FMDV serotypes O and A, which may offer a valuable tool for foot-and-mouth disease (FMD) surveillance and control programs.<\/jats:p>","DOI":"10.3390\/vetsci13080774","type":"journal-article","created":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T14:16:27Z","timestamp":1785766587000},"page":"774","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Development of a Competitive Chemiluminescent Enzyme Immunoassay Based on Recombinant FMDV VP2 Protein for Rapid and Simultaneous Detection of Antibodies Against Foot-and-Mouth Disease Virus Serotypes O and A"],"prefix":"10.3390","volume":"13","author":[{"given":"Shi\u2019ao","family":"Bian","sequence":"first","affiliation":[{"name":"Key Laboratory of Animal Medicine of Sichuan Education Department, College of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu 610041, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zongzhe","family":"Yue","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Medicine of Sichuan Education Department, College of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu 610041, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2369-6058","authenticated-orcid":false,"given":"Long","family":"Zhou","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Medicine of Sichuan Education Department, College of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu 610041, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Ai","sequence":"additional","affiliation":[{"name":"Technology Center of Kunming Customs, Kunming 650228, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2145-3706","authenticated-orcid":false,"given":"Diangang","family":"Han","sequence":"additional","affiliation":[{"name":"Technology Center of Kunming Customs, Kunming 650228, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Munib","family":"Ullah","sequence":"additional","affiliation":[{"name":"Department of Clinical Studies, Faculty of Veterinary and Animal Sciences, PMAS-Arid Agriculture University, Rawalpindi 46300, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2186-7661","authenticated-orcid":false,"given":"Zhidong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Medicine of Sichuan Education Department, College of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu 610041, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanmin","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Medicine of Sichuan Education Department, College of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu 610041, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/j.cvfa.2024.01.001","article-title":"Foot-and-mouth disease","volume":"40","author":"Arzt","year":"2024","journal-title":"Vet. 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