{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,10]],"date-time":"2026-11-10T04:03:16Z","timestamp":1794283396752,"version":"4.9.0"},"reference-count":40,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,8,8]],"date-time":"2026-08-08T00:00:00Z","timestamp":1786147200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100021171","name":"Guangdong Basic and Applied Basic Research Foundation","doi-asserted-by":"crossref","award":["2024A1515140152"],"award-info":[{"award-number":["2024A1515140152"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Key Research and Development Special Project of Henan Province","award":["251111113600"],"award-info":[{"award-number":["251111113600"]}]},{"name":"Key Scientific and Technological Grants in Henan Province","award":["24210211031"],"award-info":[{"award-number":["24210211031"]}]},{"name":"National Key Research and Development Program Projects","award":["2023YFD1801302"],"award-info":[{"award-number":["2023YFD1801302"]}]},{"name":"National Key Research and Development Program Projects","award":["2023YFD1801301"],"award-info":[{"award-number":["2023YFD1801301"]}]},{"name":"Special Funds for Rural Revitalization Strategy of Guangdong Provincial Department of Agriculture and Rural Affairs in 2024","award":["2024CXTD15"],"award-info":[{"award-number":["2024CXTD15"]}]},{"name":"Open Foundation of Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding","award":["2024B03"],"award-info":[{"award-number":["2024B03"]}]},{"name":"Base and Talent Program of Science and Technology Plan in Tibet Autonomous Region","award":["XZ202401JD0012"],"award-info":[{"award-number":["XZ202401JD0012"]}]},{"name":"Foshan University High Level Talents Research Start-up Project","award":["CGZ07465\/CGZ07001"],"award-info":[{"award-number":["CGZ07465\/CGZ07001"]}]},{"name":"Guangdong Provincial Department of Education\u2019s distinctive innovation initiative","award":["2023KTSCX128"],"award-info":[{"award-number":["2023KTSCX128"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31902279"],"award-info":[{"award-number":["31902279"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"award":["31902279"],"award-info":[{"award-number":["31902279"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vet. Sci.","Veterinary Sciences"],"abstract":"<jats:p>As a highly contagious infectious disease in the swine industry, porcine reproductive and respiratory syndrome (PRRS) can cause reproductive disorders in sows and respiratory diseases in piglets, resulting in sustained economic losses. Nonstructural protein 10 (NSP 10), as one of the core components of the replication and transcription complex of PRRS virus (PRRSV), has both helicase and adenosine triphosphatase activities, and has the potential as a diagnostic marker. In this study, NSP10 recombinant protein was used as coating antigen, and an indirect ELISA method for PRRSV NSP10 detection was established by square matrix titration. After optimizing the assay parameters, high specificity (no reactions with other pig viruses), high sensitivity (able to detect antibodies even when the positive blood sample was diluted 1280 times), and excellent reproducibility (less than 10% variation) were shown. Therefore, the NSP10 indirect enzyme-linked immunosorbent assay (ELISA) developed in this study provides an effective technical means for enhancing PRRSV surveillance in swine herds, thereby establishing a solid foundation for the clinical diagnosis and comprehensive prevention and control of PRRSV.<\/jats:p>","DOI":"10.3390\/vetsci13080793","type":"journal-article","created":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T10:23:50Z","timestamp":1786357430000},"page":"793","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Establishment of an Indirect ELISA Detection Method for Porcine Reproductive and Respiratory Syndrome Virus NSP10"],"prefix":"10.3390","volume":"13","author":[{"given":"Gan","family":"Li","sequence":"first","affiliation":[{"name":"School of Animal Science and Technology, Foshan University, Foshan 528225, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qipeng","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Animal Science and Technology, Foshan University, Foshan 528225, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojing","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Animal Science and Technology, Foshan University, Foshan 528225, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huawei","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Animal Product Quality and Safety Technology, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruining","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Animal Product Quality and Safety Technology, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-0306-0327","authenticated-orcid":false,"given":"Keshan","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Animal Science and Technology, Foshan University, Foshan 528225, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaqiong","family":"Ye","sequence":"additional","affiliation":[{"name":"School of Animal Science and Technology, Foshan University, Foshan 528225, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4143-3606","authenticated-orcid":false,"given":"Mengmeng","family":"Zhao","sequence":"additional","affiliation":[{"name":"School of Animal Science and Technology, Foshan University, Foshan 528225, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1007\/s11262-022-01957-x","article-title":"Variations in the NSP4 gene of the type 2 porcine reproductive and respiratory syndrome virus isolated in China from 1996 to 2021","volume":"59","author":"Sha","year":"2023","journal-title":"Virus Genes"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Chrun, T., Maze, E.A., Roper, K.J., Vatzia, E., Paudyal, B., McNee, A., Martini, V., Manjegowda, T., Freimanis, G., and Silesian, A. 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