{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T04:26:07Z","timestamp":1786940767906,"version":"build-2736575974"},"reference-count":30,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T00:00:00Z","timestamp":1786665600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Major Project of the Joint Research Fund of Gansu Province","award":["25JRRA1085"],"award-info":[{"award-number":["25JRRA1085"]}]},{"name":"Major Science and Technology Project of Gansu Province","award":["23ZDNA007 and 24ZD13NA008"],"award-info":[{"award-number":["23ZDNA007 and 24ZD13NA008"]}]},{"name":"Key Research and Development Program of Gansu Province","award":["25YFNA015"],"award-info":[{"award-number":["25YFNA015"]}]},{"name":"Innovation Program of Chinese Academy of Agricultural Sciences","award":["CAAS- ZDRW202510.1"],"award-info":[{"award-number":["CAAS- ZDRW202510.1"]}]},{"award":["CAAS- ZDRW202510.1"],"award-info":[{"award-number":["CAAS- ZDRW202510.1"]}],"id":[{"id":"https:\/\/ror.org\/0313jb750","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vet. Sci.","Veterinary Sciences"],"abstract":"<jats:p>Brucellosis is a zoonotic disease causing significant economic losses, and necessitating standardized diagnostic kits for effective surveillance. The objectives of this study are to investigate brucellosis serosurveillance in ruminants, and to evaluate three serological tests\u2014Rose Bengal Plate Tests (RBPTs), Serum Tube Agglutination Tests (STATs), and Enzyme-Linked Immunosorbent Assays (ELISAs)\u2014each from three manufacturers. A total of 5797 serum samples (5260 sheep, 83 bovines, and 454 spotted deer) were initially screened with the RBPT, and 54 RBPT-positive selected samples were subsequently analyzed by the STAT and ELISA. RBPT screening showed significant variability: kit A (0.86%) showed significantly lower positivity than kits B and C (1.45% and 1.43%, p &lt; 0.05). Among 54 RBPT-positive samples, STAT positivity from manufacturers A, B, and C was 61.11%, 70.37%, and 66.67%, respectively, with no significant differences (p &gt; 0.017 in pairwise comparisons). All ELISAs consistently outperformed STATs. Kit B (competitive ELISA, 90.74%) had significantly higher positivity than kits A (indirect ELISA, 77.78%) and C (competitive ELISA, 74.07%) (p &lt; 0.017 in pairwise comparisons). Key implications: Results from different manufacturers are not directly comparable, and concurrent use of different techniques on the same sample can yield discrepant outcomes. This underscores the urgent need for diagnostic kit standardization in brucellosis prevalence studies.<\/jats:p>","DOI":"10.3390\/vetsci13080804","type":"journal-article","created":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T15:48:36Z","timestamp":1786722516000},"page":"804","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comparison of Various Rose Bengal Plate Tests, Serum Tube Agglutination Tests, and Enzyme-Linked Immunosorbent Assays Used in Brucellosis Serosurveillance in Sheep, Bovines, and Spotted Deer"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-1513-6849","authenticated-orcid":false,"given":"Weldeab Solomon","family":"Ghebrezgabher","sequence":"first","affiliation":[{"name":"State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China"},{"name":"National Higher Education and Research Institute, Hamelmalo Agricultural College, Keren P.O. Box 397, Eritrea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guodong","family":"Song","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-0976-2607","authenticated-orcid":false,"given":"Jiazhen","family":"Ge","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China"},{"name":"College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yijian","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pengcheng","family":"Gao","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renge","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China"},{"name":"College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China"},{"name":"Lanzhou Animal Disease Prevention and Control Center, Lanzhou 730050, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-9151-583X","authenticated-orcid":false,"given":"Fuying","family":"Zheng","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9443-2677","authenticated-orcid":false,"given":"Yuefeng","family":"Chu","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China"},{"name":"Gansu Province Research Center for Basic Disciplines of Pathogen Biology, Lanzhou 730046, China"},{"name":"Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Ruminant Disease Prevention and Control (West), Ministry of Agricultural and Rural Affairs, Lanzhou 730046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"27","DOI":"10.20506\/rst.32.1.2180","article-title":"Brucellosis in terrestrial wildlife","volume":"32","author":"Godfroid","year":"2013","journal-title":"Rev. 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