{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T10:18:11Z","timestamp":1787739491463,"version":"build-2784847793"},"reference-count":30,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T00:00:00Z","timestamp":1787702400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vet. Sci.","Veterinary Sciences"],"abstract":"<jats:p>Blood transfusion is a life-saving intervention in canine critical care, but the clinical value of prestorage leukoreduction remains uncertain. This Critically Appraised Topic addressed whether leukoreduced canine blood products, compared with corresponding non-leukoreduced products, improve product quality, reduce recipient inflammatory responses or transfusion reactions, and improve patient-centered outcomes. PubMed, Scopus, Web of Science, and CAB Abstracts were searched from inception to 10 March 2026 using a common search strategy. Twenty-eight comparative articles were retained for appraisal after exclusion of five reports that did not contain a direct leukoreduced-versus-non-leukoreduced comparison aligned with the structured question. Most of the available evidence concerns laboratory and storage outcomes. Leukoreduction modified selected biochemical, inflammatory, oxidative, and cellular markers, but the direction and consistency of the findings varied across outcomes and studies. Clinical studies did not demonstrate a consistent reduction in transfusion reactions or improvement in survival or recovery. Current evidence therefore supports leukoreduction primarily as a blood-product processing strategy; it is insufficient to establish a patient-centered benefit from routine universal leukoreduction in dogs.<\/jats:p>","DOI":"10.3390\/vetsci13090865","type":"journal-article","created":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T09:05:46Z","timestamp":1787735146000},"page":"865","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Prestorage Leukoreduction of Canine Blood Products: A Critically Appraised Topic"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3922-6069","authenticated-orcid":false,"given":"Daniela","family":"Proverbio","sequence":"first","affiliation":[{"name":"Dipartimento di Medicina Veterinaria e Scienze Animali (DIVAS), Universit\u00e0 degli Studi di Milano, 26900 Lodi, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8241-907X","authenticated-orcid":false,"given":"Roberta","family":"Perego","sequence":"additional","affiliation":[{"name":"Dipartimento di Medicina Veterinaria e Scienze Animali (DIVAS), Universit\u00e0 degli Studi di Milano, 26900 Lodi, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3898-6955","authenticated-orcid":false,"given":"Eva","family":"Spada","sequence":"additional","affiliation":[{"name":"Dipartimento di Medicina Veterinaria e Scienze Animali (DIVAS), Universit\u00e0 degli Studi di Milano, 26900 Lodi, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Antognoni, M.T., Marenzoni, M.L., Misia, A.L., Avellini, L., Chiaradia, E., Gavazza, A., and Miglio, A. (2021). Effect of leukoreduction on hematobiochemical parameters and storage hemolysis in canine whole blood units. Animals, 11.","DOI":"10.3390\/ani11040925"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1111\/vec.12160","article-title":"Cytokine concentration in stored canine erythrocyte concentrates","volume":"24","author":"Corsi","year":"2014","journal-title":"J. Vet. Emerg. Crit. Care"},{"key":"ref_3","first-page":"214","article-title":"Leucoreduction of blood components: An effective way to increase blood safety?","volume":"14","author":"Bianchi","year":"2016","journal-title":"Blood Transfus."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Spada, E., Perego, R., Baggiani, L., and Proverbio, D. (2021). Effect of leukoreduction by pre-storage filtration on coagulation activity of canine plasma collected for transfusion. Vet. Sci., 8.","DOI":"10.3390\/vetsci8080157"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"890","DOI":"10.2460\/ajvr.82.11.890","article-title":"Effects of leukoreduction on N-methylhistamine concentration in stored units of canine whole blood","volume":"82","author":"Frank","year":"2021","journal-title":"Am. J. Vet. Res."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2001","DOI":"10.2460\/ajvr.73.12.2001","article-title":"Effect of leukoreduction treatment on vascular endothelial growth factor concentration in stored canine blood transfusion products","volume":"73","author":"Graf","year":"2012","journal-title":"Am. J. Vet. Res."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1111\/vcp.12034","article-title":"Microparticles in stored canine RBC concentrates","volume":"42","author":"Herring","year":"2013","journal-title":"Vet. Clin. Pathol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1186\/s13071-020-3991-9","article-title":"Survival of Rickettsia conorii in artificially contaminated whole and leukoreduced canine blood units during the storage period","volume":"13","author":"Lucchese","year":"2020","journal-title":"Parasit. Vectors"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1111\/jvim.14664","article-title":"Effects of leukoreduction and storage on erythrocyte phosphatidylserine expression and eicosanoid concentrations in units of canine packed red blood cells","volume":"31","author":"Muro","year":"2017","journal-title":"J. Vet. Intern. Med."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"35","DOI":"10.2460\/ajvr.76.1.35","article-title":"Procoagulant phospholipid concentration in canine erythrocyte concentrates stored with or without prestorage leukoreduction","volume":"76","author":"Smith","year":"2015","journal-title":"Am. J. Vet. Res."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"936","DOI":"10.1111\/jvim.16039","article-title":"Effects of leukoreduction on storage lesions in whole blood and blood components of dogs","volume":"35","author":"Stefani","year":"2021","journal-title":"J. Vet. Intern. Med."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"836","DOI":"10.1111\/vec.13225","article-title":"The effects of leukoreduction on canine blood unit weight and processing time","volume":"32","author":"Trinder","year":"2022","journal-title":"J. Vet. Emerg. Crit. Care"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1111\/vec.12857","article-title":"A pilot study evaluating the effects of prestorage leukoreduction on markers of inflammation in critically ill dogs receiving a blood transfusion","volume":"29","author":"Blois","year":"2019","journal-title":"J. Vet. Emerg. Crit. Care"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1248","DOI":"10.1111\/jvim.16487","article-title":"Effect of leukoreduction on inflammation in critically ill dogs receiving red blood cell transfusions: A randomized blinded controlled clinical trial","volume":"36","author":"Claus","year":"2022","journal-title":"J. Vet. Intern. Med."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1111\/vec.13175","article-title":"The influence of leukoreduction on the acute transfusion-related complication rate in 455 dogs receiving 730 packed RBCs: 2014\u20132017","volume":"32","author":"Davidow","year":"2022","journal-title":"J. Vet. Emerg. Crit. Care"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1111\/vox.12058","article-title":"Transfusion of 28-day-old leukoreduced or non-leukoreduced stored red blood cells induces an inflammatory response in healthy dogs","volume":"105","author":"Callan","year":"2013","journal-title":"Vox Sang."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1131","DOI":"10.1111\/j.1939-1676.2010.0561.x","article-title":"Effect of leukoreduction on transfusion-induced inflammation in dogs","volume":"24","author":"McMichael","year":"2010","journal-title":"J. Vet. Intern. Med."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1325","DOI":"10.1111\/jvim.16138","article-title":"Randomized double-blinded clinical trial on acute transfusion reactions in dogs receiving leukoreduced versus nonleukoreduced packed red blood cells","volume":"35","author":"Radulescu","year":"2021","journal-title":"J. Vet. Intern. Med."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Steblaj, B., Galli, J., Torgerson, P., and Kutter, A. (2023). Evaluation of leukocyte depletion of packed red blood cell units and impact on clinically observed transfusion reactions. Front. Vet. Sci., 10.","DOI":"10.3389\/fvets.2023.1217575"},{"key":"ref_20","first-page":"711","article-title":"The effects of prestorage leukoreduction and storage duration on the in vitro quality of canine packed red blood cells","volume":"36","author":"Ekiz","year":"2012","journal-title":"Turk. J. Vet. Anim. Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1894","DOI":"10.1111\/jvim.15876","article-title":"Neutrophil extracellular traps in stored canine red blood cell units","volume":"34","author":"McQuinn","year":"2020","journal-title":"J. Vet. Intern. Med."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Miglio, A., Barbetta, A., Cremonini, V., Barbato, O., Ricci, G., Toppi, V., Avellini, L., Cavani, V., and Antognoni, M.T. (2025). Time-dependent changes in malondialdehyde and free-hemoglobin in leukoreduced and non-leukoreduced canine packed red blood cells units during storage. Vet. Sci., 12.","DOI":"10.3390\/vetsci12090838"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1498","DOI":"10.1111\/jvim.17031","article-title":"Effect of leukoreduction on the omics phenotypes of canine packed red blood cells during refrigerated storage","volume":"38","author":"Miglio","year":"2024","journal-title":"J. Vet. Intern. Med."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"969","DOI":"10.2460\/ajvr.76.11.969","article-title":"Effect of leukoreduction on concentrations of interleukin-8, interleukin-1\u03b2, and tumor necrosis factor-\u03b1 in canine packed red blood cells during storage","volume":"76","author":"Purcell","year":"2015","journal-title":"Am. J. Vet. Res."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Shin, S.W., Kim, M., Shin, C., Bae, H., Park, J., Jung, D.-I., Cho, K.-W., and Yu, D. (2025). Understanding the biochemical impact of leukoreduction on canine pRBC storage: A focus on reactive oxygen species and storage lesions. Front. Vet. Sci., 12.","DOI":"10.3389\/fvets.2025.1563532"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2495","DOI":"10.1111\/jvim.17175","article-title":"A prospective multicenter observational study assessing incidence and risk factors for acute blood transfusion reactions in dogs","volume":"38","author":"Hall","year":"2024","journal-title":"J. Vet. Intern. Med."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"846","DOI":"10.2460\/ajvr.80.9.846","article-title":"Coagulation factor activity in units of leukoreduced and nonleukoreduced canine fresh-frozen plasma","volume":"80","author":"Foote","year":"2019","journal-title":"Am. J. Vet. Res."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"251","DOI":"10.56808\/2985-1130.2829","article-title":"Effects of cryopreservation and leukodepletion on morphological index of red blood cells in dogs","volume":"47","author":"Bala","year":"2017","journal-title":"Thai J. Vet. Med."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"72","DOI":"10.4142\/jvs.2019.20.1.72","article-title":"Effects of irradiation and leukoreduction on down-regulation of CXCL-8 and storage lesion in stored canine whole blood","volume":"20","author":"Yang","year":"2019","journal-title":"J. Vet. Sci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1111\/vec.13045","article-title":"Association of Veterinary Hematology and Transfusion Medicine (AVHTM) Transfusion Reaction Small Animal Consensus Statement (TRACS) Part 2: Prevention and Monitoring","volume":"31","author":"Davidow","year":"2021","journal-title":"J. Vet. Emerg. Crit. 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