{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T12:24:20Z","timestamp":1788265460208,"version":"build-2803163510"},"reference-count":54,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T00:00:00Z","timestamp":1787961600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Jiangsu Bioperfectus Technologies Co., Ltd.","award":["21600-3-2004-HXXM02080"],"award-info":[{"award-number":["21600-3-2004-HXXM02080"]}]},{"DOI":"10.13039\/100012547","name":"Guangxi Natural Science Foundation","doi-asserted-by":"publisher","award":["2023GXNSFAA026273"],"award-info":[{"award-number":["2023GXNSFAA026273"]}],"id":[{"id":"10.13039\/100012547","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vet. Sci.","Veterinary Sciences"],"abstract":"<jats:p>Streptococcus suis (S. suis) is a zoonotic pathogen that causes severe diseases including meningitis, septic shock, and endocarditis in both pigs and humans. In swine, S. suis is one of the most important bacterial pathogens causing invasive diseases such as meningitis, septicemia, arthritis, and sudden death, with serotypes 1, 2, 7, and 9 being among the most frequently recovered from clinical cases in affected herds across Southeast Asia. We developed a multiplex TaqMan qPCR assay targeting four serotype-specific genes (cps1I, cps2I, cps7L, and cps9J) for detection of major S. suis serotypes in porcine samples. The assay achieved a detection limit of 10 copies\/\u03bcL for all four target serogroups, amplification efficiencies of 89.64% to 100.42% (R2 \u2265 0.997), and no cross-reactivity with any of the negative controls, including SS4, APP, HPS, Salmonella, E. coli, and S. aureus. Intra-assay and inter-assay repeatability were below 3%. Testing 98 clinical porcine samples from Guangxi, China, yielded positivity rates of 26.53% for the serotype 1\/14 group, 19.39% for the serotype 2\/1\/2 group, 17.34% for serotype 7, and 29.59% for serotype 9, respectively, with mixed-target detection in 30.61% of samples. This assay enables rapid, reliable detection of major zoonotic S. suis serotypes in porcine samples, though it detects serotypes 1 and 14 as a group, and serotypes 2 and 1\/2 as a group, due to capsular locus sequence similarity\u2014a limitation acceptable for surveillance given the high pathogenicity within each group. This tool supports One Health-based veterinary surveillance for zoonotic disease control.<\/jats:p>","DOI":"10.3390\/vetsci13090884","type":"journal-article","created":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T11:33:32Z","timestamp":1788262412000},"page":"884","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Multiplex TaqMan qPCR Assay for Rapid Detection of Streptococcus suis Serotypes 1, 2, 7, and 9 with Cross-Reactivity to Serotypes 14 and 1\/2"],"prefix":"10.3390","volume":"13","author":[{"given":"Jingye","family":"Huang","sequence":"first","affiliation":[{"name":"Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning 530004, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"given":"Tingting","family":"Yue","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning 530004, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"given":"Jianing","family":"Huang","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning 530004, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"software","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"given":"Qiong","family":"Chen","sequence":"additional","affiliation":[{"name":"Nanning Zhufulai Animal Health Management Co., Ltd., Nanning 530006, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"}]},{"given":"Min","family":"Zheng","sequence":"additional","affiliation":[{"name":"Guangxi Center for Animal Disease Control and Prevention, Nanning 530001, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"visualization","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9517-9802","authenticated-orcid":false,"given":"Meiling","family":"Yu","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning 530004, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"}]},{"given":"Mingsheng","family":"Jiang","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning 530004, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"visualization","vocabulary":"credit"}]},{"given":"Xiulin","family":"Li","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning 530004, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1733-5267","authenticated-orcid":false,"given":"Hailan","family":"Chen","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning 530004, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Segura, M., Aragon, V., Brockmeier, S., Gebhart, C., Greeff, A., Kerdsin, A., O\u2019Dea, M., Okura, M., Sal\u00e9ry, M., and Schultsz, C. (2020). Update on Streptococcus suis Research and Prevention in the Era of Antimicrobial Restriction: 4th International Workshop on S. suis. Pathogens, 9.","DOI":"10.3390\/pathogens9050374"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"683","DOI":"10.26444\/aaem\/79813","article-title":"Streptococcus suis: A re-emerging pathogen associated with occupational exposure to pigs or pork products. Part I\u2014Epidemiology","volume":"24","author":"Dutkiewicz","year":"2017","journal-title":"Ann. Agric. Environ. Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/S1473-3099(07)70001-4","article-title":"Streptococcus suis: An emerging zoonotic pathogen","volume":"7","author":"Lun","year":"2007","journal-title":"Lancet Infect. Dis."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1016\/j.diagmicrobio.2010.12.015","article-title":"Real-time PCR for detection of Streptococcus suis serotype 2 in cerebrospinal fluid of human patients with meningitis","volume":"70","author":"Nga","year":"2011","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1086\/527385","article-title":"Streptococcus suis Meningitis in Adults in Vietnam","volume":"46","author":"Campbell","year":"2008","journal-title":"Clin. Infect. Dis."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.vetmic.2005.01.003","article-title":"Biochemical analysis, cpn60 and 16S rDNA sequence data indicate that Streptococcus suis serotypes 32 and 34, isolated from pigs, are Streptococcus orisratti","volume":"107","author":"Hill","year":"2005","journal-title":"Vet. Microbiol."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Okura, M., Osaki, M., Nomoto, R., Arai, S., Osawa, R., Sekizaki, T., and Takamatsu, D. (2016). Current Taxonomical Situation of Streptococcus suis. Pathogens, 5.","DOI":"10.3390\/pathogens5030045"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"734543","DOI":"10.1016\/j.aquaculture.2019.734543","article-title":"Molecular characterization and pathogenicity of Streptococcus agalactiae serotypes Ia ST7 and III ST283 isolated from cultured red hybrid tilapia in Malaysia","volume":"515","author":"Syuhada","year":"2020","journal-title":"Aquaculture"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2796","DOI":"10.1128\/AEM.03742-12","article-title":"Genetic Analysis of Capsular Polysaccharide Synthesis Gene Clusters from All Serotypes of Streptococcus suis: Potential Mechanisms for Generation of Capsular Variation","volume":"79","author":"Okura","year":"2013","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_10","first-page":"e45","article-title":"Streptococcus suis, an important pig pathogen and emerging zoonotic agent\u2014An update on the worldwide distribution based on serotyping and sequence typing","volume":"3","author":"Auger","year":"2014","journal-title":"Emerg. Microbes Infect."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Wang, H., Chen, J., Sun, Y., An, T., Wang, Y., Chen, H., Yu, C., Xia, C., and Zhang, H. (2024). Development and application of a quadruplex TaqMan fluorescence quantitative PCR typing method for Streptococcus suis generalis, type 2, type 7 and type 9. Front. Cell. Infect. Microbiol., 14.","DOI":"10.3389\/fcimb.2024.1475878"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"917","DOI":"10.1007\/s10096-016-2616-x","article-title":"Streptococcus suis in invasive human infections in Poland: Clonality and determinants of virulence and antimicrobial resistance","volume":"35","author":"Bojarska","year":"2016","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Yang, H., Huang, J., Hu, X., Hu, M., Zhang, Q., and Jin, M. (2021). Comparative Genome Analysis of Streptococcus suis Serotype 9 Isolates from China, The Netherland, and the U.K.. Life, 11.","DOI":"10.3390\/life11121324"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1186\/s13567-025-01608-7","article-title":"Characterization of an enterococcal phage endolysin as a potential antimicrobial agent against Streptococcus suis","volume":"56","author":"Wang","year":"2025","journal-title":"Vet. Res."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Kaszowska, M., Majkowska-Skrobek, G., Markwitz, P., Lood, C., Jachymek, W., Maciejewska, A., Lukasiewicz, J., and Drulis-Kawa, Z. (2021). The Mutation in wbaP cps Gene Cluster Selected by Phage-Borne Depolymerase Abolishes Capsule Production and Diminishes the Virulence of Klebsiella pneumoniae. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms222111562"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Dekker, N., Daemen, I., Verstappen, K., De Greeff, A., Smith, H., and Duim, B. (2016). Simultaneous Quantification and Differentiation of Streptococcus suis Serotypes 2 and 9 by Quantitative Real-Time PCR, Evaluated in Tonsillar and Nasal Samples of Pigs. Pathogens, 5.","DOI":"10.3390\/pathogens5030046"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Yang, D., Xu, J., Hu, M., Zhu, J., Ren, B., Huang, X., and Wang, L. (2026). Epidemiological investigation and pathogenicity of Streptococcus suis in eastern China. Front. Microbiol., 16.","DOI":"10.3389\/fmicb.2025.1710390"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"101098","DOI":"10.1016\/j.onehlt.2025.101098","article-title":"Streptococcus suis: Epidemiology and resistance evolution of an emerging zoonotic bacteria","volume":"21","author":"Liu","year":"2025","journal-title":"One Health"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"197985","DOI":"10.1016\/j.virusres.2020.197985","article-title":"Swine hepatitis E virus: Cross-species infection, pork safety and chronic infection","volume":"284","author":"Sooryanarain","year":"2020","journal-title":"Virus Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"37788","DOI":"10.1038\/s41598-025-21437-0","article-title":"Pathogenic characteristics of an unencapsulated Streptococcus suis serotype 31 strain isolated from a patient in Thailand","volume":"15","author":"Boueroy","year":"2025","journal-title":"Sci. Rep."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"28984","DOI":"10.1038\/srep28984","article-title":"An emerging zoonotic clone in the Netherlands provides clues to virulence and zoonotic potential of Streptococcus suis","volume":"6","author":"Willemse","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_22","first-page":"001590","article-title":"Genomics of Irish swine-derived Streptococcus suis: Population structure, prophages and anti-viral defence mechanisms","volume":"12","author":"Osei","year":"2026","journal-title":"Microb. Genom."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1186\/s13567-020-00813-w","article-title":"Population structure, genetic diversity and pathotypes of Streptococcus suis isolated during the last 13 years from diseased pigs in Switzerland","volume":"51","author":"Scherrer","year":"2020","journal-title":"Vet. Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1016\/j.vetmic.2008.09.074","article-title":"Correlating blood immune parameters and a CCT7 genetic variant with the shedding of Salmonella enterica serovar Typhimurium in swine","volume":"135","author":"Uthe","year":"2009","journal-title":"Vet. Microbiol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"165","DOI":"10.14202\/vetworld.2019.165-169","article-title":"Contamination of Streptococcus suis in pork and edible pig organs in central Thailand","volume":"12","author":"Boonyong","year":"2019","journal-title":"Vet. World"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1007\/s11259-026-11271-8","article-title":"Streptococcus suis in Asia: Epidemiology, transmission and zoonotic risk at the animal\u2013human interface","volume":"50","author":"Tan","year":"2026","journal-title":"Vet. Res. Commun."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Liu, Z., Zheng, H., Gottschalk, M., Bai, X., Lan, R., Ji, S., Liu, H., and Xu, J. (2013). Development of Multiplex PCR Assays for the Identification of the 33 Serotypes of Streptococcus suis. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0072070"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1007\/s11783-011-0302-x","article-title":"Design and use of group-specific primers and probes for real-time quantitative PCR","volume":"5","author":"Lim","year":"2011","journal-title":"Front. Environ. Sci. Eng. China"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Su, Y., Meng, J., Zhao, M., Li, C., Zhai, S., Li, Y., Chu, P., Bian, Z., Zhang, K., and Yang, D. (2024). Rapid detection of zoonotic Streptococcus suis serotype 2 and 14 by enzyme-activated probe fluorescence quantitative PCR method. BMC Vet. Res., 20.","DOI":"10.1186\/s12917-024-04361-9"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/S0378-1135(00)00188-7","article-title":"Distribution of capsular types and production of muramidase-released protein (MRP) and extracellular factor (EF) of Streptococcus suis strains isolated from diseased pigs in seven European countries","volume":"74","author":"Wisselink","year":"2000","journal-title":"Vet. Microbiol."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Thu, I.S.L., Tragoolpua, K., Intorasoot, S., Anukool, U., Khamnoi, P., Kerdsin, A., and Tharinjaroen, C.S. (2021). Direct Detection of Streptococcus suis from Cerebrospinal Fluid, Positive Hemoculture, and Simultaneous Differentiation of Serotypes 1, 1\/2, 2, and 14 within Single Reaction. Pathogens, 10.","DOI":"10.3390\/pathogens10080996"},{"key":"ref_32","first-page":"3558","article-title":"The presence and serological types of streptococcus suis strains isolated from pigs originating from some farms in serbia","volume":"21","author":"Stanojkovic","year":"2012","journal-title":"Fresenius Environ. Bull."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"001950","DOI":"10.1099\/jmm.0.001950","article-title":"Modified multiplex PCR for serotyping and pathotyping of Streptococcus suis","volume":"74","author":"Hatrongjit","year":"2025","journal-title":"J. Med. Microbiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"110265","DOI":"10.1016\/j.vetmic.2024.110265","article-title":"Streptococcus suis strains with novel and previously undescribed capsular loci circulate in Europe","volume":"298","author":"Fittipaldi","year":"2024","journal-title":"Vet. Microbiol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.carres.2016.07.005","article-title":"Structure determination of Streptococcus suis serotype 9 capsular polysaccharide and assignment of functions of the cps locus genes involved in its biosynthesis","volume":"433","author":"Vinogradov","year":"2016","journal-title":"Carbohydr. Res."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"995","DOI":"10.1111\/tbed.13123","article-title":"Identification of six novel capsular polysaccharide loci (NCL) from Streptococcus suis multidrug resistant non-typeable strains and the pathogenic characteristic of strains carrying new NCLs","volume":"66","author":"Huang","year":"2019","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"E2495","DOI":"10.1111\/tbed.14592","article-title":"The characteristics of population structure and antimicrobial resistance of Streptococcus suis serotype 8, a non-negligible pathotype","volume":"69","author":"Liu","year":"2022","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1007\/s00253-026-13828-8","article-title":"Ribosomal protein RPSA is a promising target for the prevention of Streptococcus suis serotype 2","volume":"110","author":"Liu","year":"2026","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Tan, M., Tan, J., Zhang, F., Li, H., Ji, H., Fang, S., Wu, C., Rao, Y., Zeng, Y., and Yang, Q. (2022). Exogenous glycogen utilization effects the transcriptome and pathogenicity of Streptococcus suis serotype 2. Front. Cell. Infect. Microbiol., 12.","DOI":"10.3389\/fcimb.2022.938286"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"e995","DOI":"10.1002\/mbo3.995","article-title":"Rapid high resolution melting assay to differentiate Streptococcus suis serotypes 2, 1\/2, 1, and 14","volume":"9","author":"Scherrer","year":"2020","journal-title":"MicrobiologyOpen"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Zhang, J., Zhang, D., Jiang, J., Lin, Y., Wan, C., and Che, Y. (2026). Development and Validation of a High-Resolution Melting (HRM) Method for Differentiating Ovis and Equi Biovars of Corynebacterium pseudotuberculosis. Vet. Sci., 13.","DOI":"10.3390\/vetsci13040372"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1513","DOI":"10.1007\/s00217-007-0683-z","article-title":"Modelling the limit of detection in real-time quantitative PCR","volume":"226","author":"Burns","year":"2008","journal-title":"Eur. Food Res. Technol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"4111","DOI":"10.1128\/AEM.00315-15","article-title":"Eight Novel Capsular Polysaccharide Synthesis Gene Loci Identified in Nontypeable Streptococcus suis Isolates","volume":"81","author":"Zheng","year":"2015","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Hatrongjit, R., Fittipaldi, N., Gottschalk, M., and Kerdsin, A. (2020). Tools for Molecular Epidemiology of Streptococcus suis. Pathogens, 9.","DOI":"10.3390\/pathogens9020081"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Chidkoksung, K., Parakasikron, N., Nuanualsuwan, S., and Khantasup, K. (2024). Development of a latex agglutination test based on VH antibody fragment for detection of Streptococcus suis serotype 2. PLoS ONE, 19.","DOI":"10.1371\/journal.pone.0299691"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1007\/s40588-017-0055-9","article-title":"Identification and Detection of Serotype-Specific Genes: Effective Serotyping of Streptococcus suis","volume":"4","author":"Wang","year":"2017","journal-title":"Curr. Clin. Microbiol. Rep."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Kunpatee, K., Khantasup, K., Komolpis, K., Yakoh, A., Nuanualsuwan, S., Sain, M.M., and Chaiyo, S. (2023). Ratiometric electrochemical lateral flow immunoassay for the detection of Streptococcus suis serotype 2. Biosens. Bioelectron., 242.","DOI":"10.1016\/j.bios.2023.115742"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1016\/S0034-5288(18)30634-9","article-title":"An evaluation of five serological tests for the detection of antibody to bovine herpesvirus 1 in vaccinated and experimentally infected cattle","volume":"41","author":"Edwards","year":"1986","journal-title":"Res. Vet. Sci."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"865","DOI":"10.1007\/s11250-010-9774-0","article-title":"Assessment of milk ring test and some serological tests in the detection of Brucella melitensis in Syrian female sheep","volume":"43","author":"Ramadan","year":"2011","journal-title":"Trop. Anim. Health Prod."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1925","DOI":"10.3201\/eid1412.080162","article-title":"Occupational Exposure to a Streptococcus suis among US Swine Workers","volume":"14","author":"Smith","year":"2008","journal-title":"Emerg. Infect. Dis."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Matajira, C.E.C., Moreno, L.Z., Poor, A.P., Gomes, V.T.M., Dalmutt, A.C., Parra, B.M., Oliveira, C.H.d., Barbosa, M.R.F., Sato, M.I.Z., and Calderaro, F.F. (2019). Streptococcus suis in Brazil: Genotypic, Virulence, and Resistance Profiling of Strains Isolated from Pigs between 2001 and 2016. Pathogens, 9.","DOI":"10.3390\/pathogens9010031"},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Petrocchi Rilo, M., Guti\u00e9rrez Mart\u00edn, C.B., Acebes Fern\u00e1ndez, V., Aguar\u00f3n Turrientes, \u00c1., Gonz\u00e1lez Fern\u00e1ndez, A., Migu\u00e9lez P\u00e9rez, R., and Mart\u00ednez Mart\u00ednez, S. (2024). Streptococcus suis Research Update: Serotype Prevalence and Antimicrobial Resistance Distribution in Swine Isolates Recovered in Spain from 2020 to 2022. Vet. Sci., 11.","DOI":"10.3390\/vetsci11010040"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"108750","DOI":"10.1016\/j.vetmic.2020.108750","article-title":"Capsular serotypes, antimicrobial susceptibility, and the presence of transferable oxazolidinone resistance genes in Streptococcus suis isolated from healthy pigs in China","volume":"247","author":"Zhang","year":"2020","journal-title":"Vet. Microbiol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"6513","DOI":"10.1038\/s41598-021-85882-3","article-title":"Capsular polysaccharide switching in Streptococcus suis modulates host cell interactions and virulence","volume":"11","author":"Okura","year":"2021","journal-title":"Sci. Rep."}],"container-title":["Veterinary Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-7381\/13\/9\/884\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T12:03:05Z","timestamp":1788264185000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-7381\/13\/9\/884"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,29]]},"references-count":54,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["vetsci13090884"],"URL":"https:\/\/doi.org\/10.3390\/vetsci13090884","relation":{},"ISSN":["2306-7381"],"issn-type":[{"value":"2306-7381","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,29]]}}}