{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T12:24:28Z","timestamp":1788265468829,"version":"build-2803163510"},"reference-count":34,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T00:00:00Z","timestamp":1788134400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vet. Sci.","Veterinary Sciences"],"abstract":"<jats:p>Antimicrobial resistance is an increasing concern in veterinary medicine and within the One Health framework, yet long-term data from companion animals remain limited in Jordan. This retrospective study characterized bacterial pathogens and archived antimicrobial susceptibility patterns among clinical samples from dogs and cats presented to the Veterinary Teaching Hospital of Jordan University of Science and Technology between 2012 and 2024. Records from 232 clinical cases comprising 248 submitted samples were reviewed. Among 237 samples with known culture outcomes, 121 yielded bacterial growth (51.1%), resulting in 141 bacterial isolates. Escherichia coli (E. coli) (33.3%) was the most frequently recovered organism, followed by Staphylococcus aureus (S. aureus) (19.1%), Staphylococcus spp. (15.6%), and Proteus spp. (9.9%). AST results were available for 96\/141 isolates (68.1%). Archived interpretations showed frequent non-susceptibility to several tested agents, whereas fluoroquinolone non-susceptibility was generally lower. Using the historical study rule of non-susceptibility to at least one agent in three or more antimicrobial classes, 87\/96 isolates (90.6%) met the screening criterion. However, intermediate results were grouped with resistant results, AST panels and breakpoint standards varied over 13 years, and the archived records did not permit reliable exclusion of intrinsic resistance for every organism\u2013drug combination. Accordingly, the MDR estimate is descriptive and should not be interpreted as a standardized contemporary prevalence. These data provide a baseline for companion-animal bacterial surveillance in Jordan and support prospective standardized AST and antimicrobial stewardship.<\/jats:p>","DOI":"10.3390\/vetsci13090892","type":"journal-article","created":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T11:33:32Z","timestamp":1788262412000},"page":"892","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Antimicrobial Susceptibility and Apparent Multidrug Resistance Screening Patterns of Bacterial Pathogens Recovered from Clinical Samples of Companion Animals in Jordan, 2012\u20132024: A Retrospective Study from a Veterinary Teaching Hospital"],"prefix":"10.3390","volume":"13","author":[{"given":"Mays","family":"Malkawi","sequence":"first","affiliation":[{"name":"Faculty of Veterinary Medicine, Jordan University of Science and Technology, Irbid 22110, Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mousa","family":"Daradka","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Jordan University of Science and Technology, Irbid 22110, Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7267-8270","authenticated-orcid":false,"given":"Zuhair Bani","family":"Ismail","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Jordan University of Science and Technology, Irbid 22110, Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,31]]},"reference":[{"key":"ref_1","first-page":"2198","article-title":"Antibacterial Drug Therapy","volume":"Volume 1","author":"Ettinger","year":"2024","journal-title":"Ettinger\u2019s Textbook of Veterinary Internal Medicine"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"106374","DOI":"10.1016\/j.tvjl.2025.106374","article-title":"Antimicrobial dispensing for common conditions in dogs and cats at a large veterinary practice network, 2023","volume":"312","author":"Weese","year":"2025","journal-title":"Vet. J."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Moon, D.-C., Choi, J.-H., Boby, N., Kang, H.-Y., Kim, S.-J., Song, H.-J., Park, H.-S., Gil, M.-C., Yoon, S.-S., and Lim, S.-K. (2022). Bacterial prevalence in skin, urine, diarrheal stool, and respiratory samples from dogs. Microorganisms, 10.","DOI":"10.3390\/microorganisms10081668"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1111\/jsap.13615","article-title":"Antibiotic therapy in dogs and cats in general practice in the United Kingdom before referral","volume":"64","author":"Sharman","year":"2023","journal-title":"J. Small Anim. Pract."},{"key":"ref_5","first-page":"46","article-title":"International Society for Companion Animal Infectious Diseases (ISCAID) guidelines for the diagnosis and management of bacterial urinary tract infections in dogs and cats","volume":"13","author":"Weese","year":"2021","journal-title":"J. Jpn. Assoc. Vet. Nephrol. Urol."},{"key":"ref_6","first-page":"957","article-title":"Public health risk of antimicrobial resistance transfer from companion animals","volume":"72","author":"Pomba","year":"2017","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Ekakoro, J.E., Hendrix, G.K., Guptill, L.F., and Ruple, A. (2022). Antimicrobial susceptibility and risk factors for resistance among Escherichia coli isolated from canine specimens submitted to a diagnostic laboratory in Indiana, 2010\u20132019. PLoS ONE, 17.","DOI":"10.1101\/2022.01.31.22270221"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Yudhanto, S., Hung, C.-C., Maddox, C.W., and Varga, C. (2022). Antimicrobial resistance in bacteria isolated from canine urine samples submitted to a veterinary diagnostic laboratory, Illinois, United States. Front. Vet. Sci., 9.","DOI":"10.3389\/fvets.2022.867784"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Ruzante, J.M., Harris, B., Plummer, P., Raineri, R.R., Loy, J.D., Jacob, M., Sahin, O., and Kreuder, A.J. (2022). Surveillance of antimicrobial resistance in veterinary medicine in the United States: Current efforts, challenges, and opportunities. Front. Vet. Sci., 9.","DOI":"10.3389\/fvets.2022.1068406"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Pro\u0161i\u0107, I., Vejnovi\u0107, B., Mi\u0161i\u0107, D., Radalj, A., Nik\u0161i\u0107, A., Aksentijevi\u0107, K., Radoji\u010di\u0107, M., Gajdov, V., Ili\u0107, M., and Mil\u010di\u0107 Mati\u0107, N. (2025). Antibiotic resistance patterns of bacteria isolated from canine skin and ear infections in Serbia. Antibiotics, 15.","DOI":"10.3390\/antibiotics15010021"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"101096","DOI":"10.1016\/j.onehlt.2025.101096","article-title":"Comparative study on the time trends of antimicrobial resistance at animal and human hospitals in a shared community","volume":"20","author":"Blackstock","year":"2025","journal-title":"One Health"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1111\/jvim.12562","article-title":"ACVIM consensus statement on therapeutic antimicrobial use in animals and antimicrobial resistance","volume":"29","author":"Weese","year":"2015","journal-title":"J. Vet. Intern. Med."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"108540","DOI":"10.1016\/j.vetmic.2019.108540","article-title":"Antimicrobial resistance of bacterial pathogens isolated from canine urinary tract infections","volume":"241","author":"Yu","year":"2020","journal-title":"Vet. Microbiol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"e000345","DOI":"10.1136\/vetreco-2019-000345","article-title":"Prevalence of bacteria and changes in trends in antimicrobial resistance of Escherichia coli isolated from positive canine urinary samples from an Australian referral hospital over a 5-year period (2013\u20132017)","volume":"6","author":"Roberts","year":"2019","journal-title":"Vet. Rec. Open"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Scarborough, R., Bailey, K., Galgut, B., Williamson, A., Hardefeldt, L., Gilkerson, J., and Browning, G. (2020). Use of local antibiogram data and antimicrobial importance ratings to select optimal empirical therapies for urinary tract infections in dogs and cats. Antibiotics, 9.","DOI":"10.3390\/antibiotics9120924"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1389","DOI":"10.1111\/jvim.16103","article-title":"Characterization of antimicrobial-resistant Escherichia coli causing urinary tract infections in dogs: Passive surveillance in Saskatchewan, Canada 2014 to 2018","volume":"35","author":"Courtice","year":"2021","journal-title":"J. Vet. Intern. Med."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"408205","DOI":"10.1155\/2015\/408205","article-title":"Multidrug resistance in Escherichia coli strains isolated from infections in dogs and cats in Poland (2007\u20132013)","volume":"2015","author":"Rzewuska","year":"2015","journal-title":"Sci. World J."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Haulisah, N.A., Hassan, L., Jajere, S.M., Ahmad, N.I., and Bejo, S.K. (2022). High prevalence of antimicrobial resistance and multidrug resistance among bacterial isolates from diseased pets: Retrospective laboratory data (2015\u20132017). PLoS ONE, 17.","DOI":"10.1371\/journal.pone.0277664"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Tartor, Y.H., Ammar, A.M., Abdelkhalek, A., Hassan, K.A., Shaker, A., Elnahriry, S.S., Nekouei, O., and Elsohaby, I. (2024). Emergence of pandrug-resistant carbapenemase-producing Enterobacterales in dogs and cats: A cross-sectional study in Egypt. Front. Cell. Infect. Microbiol., 14.","DOI":"10.3389\/fcimb.2024.1318585"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Momani, S., Alyahya, M.S., Zayed, D.K., Tarif, A.B., Nimri, O.F., Alshaikh, S., Belbiesi, A., and Al-Tammemi, A.a.B. (2025). Evaluating Jordan\u2019s antimicrobial resistance national action plan (2018\u20132022) implementation: Progress and recommendations. BMC Public Health, 25.","DOI":"10.1186\/s12889-025-24073-5"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"2324","DOI":"10.3168\/jds.2017-13665","article-title":"Major diseases, extensive misuse, and high antimicrobial resistance of Escherichia coli in large- and small-scale dairy cattle farms in Jordan","volume":"101","author":"Obaidat","year":"2018","journal-title":"J. Dairy Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"902","DOI":"10.1016\/j.apjtb.2015.06.015","article-title":"Molecular study on methicillin-resistant Staphylococcus aureus strains isolated from dogs and associated personnel in Jordan","volume":"5","author":"Tarazi","year":"2015","journal-title":"Asian Pac. J. Trop. Biomed."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Reddy, C.A., Beveridge, T.J., Breznak, J.A., and Marzluf, G. (2007). Methods for General and Molecular Microbiology, American Society for Microbiology Press. [3rd ed.].","DOI":"10.1128\/9781555817497"},{"key":"ref_24","first-page":"1","article-title":"Kirby-Bauer disk diffusion susceptibility test protocol","volume":"15","author":"Hudzicki","year":"2009","journal-title":"Am. Soc. Microbiol."},{"key":"ref_25","unstructured":"Wikler, M.A., Cockerill, F.R., and Craig, W.A. (2008). Performance Standards for Antimicrobial Susceptibility Testing: Eighteenth Informational Supplement, Clinical and Laboratory Standards Institute. M100-S18."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1111\/j.1469-0691.2011.03570.x","article-title":"Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: An international expert proposal for interim standard definitions for acquired resistance","volume":"18","author":"Magiorakos","year":"2012","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Girlich, D., Bonnin, R.A., Dortet, L., and Naas, T. (2020). Genetics of acquired antibiotic resistance genes in Proteus spp. Front. Microbiol., 11.","DOI":"10.3389\/fmicb.2020.00256"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"G\u00f3mez-Beltr\u00e1n, D.A., Villar, D., L\u00f3pez-Osorio, S., Ferguson, D., Monsalve, L.K., and Chaparro-Guti\u00e9rrez, J.J. (2020). Prevalence of antimicrobial resistance in bacterial isolates from dogs and cats in a veterinary diagnostic laboratory in Colombia from 2016\u20132019. Vet. Sci., 7.","DOI":"10.3390\/vetsci7040173"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Li, Y., Fern\u00e1ndez, R., Dur\u00e1n, I., Molina-L\u00f3pez, R.A., and Darwich, L. (2021). Antimicrobial resistance in bacteria isolated from cats and dogs from the Iberian Peninsula. Front. Microbiol., 11.","DOI":"10.3389\/fmicb.2020.621597"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"6695493","DOI":"10.1155\/2023\/6695493","article-title":"Monitoring the Prevalence of Antimicrobial Resistance in Companion Animals: Results from Clinical Isolates in an Italian University Veterinary Hospital","volume":"2023","author":"Scarpellini","year":"2023","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"S6","DOI":"10.2460\/javma.24.10.0657","article-title":"The diagnostic process for infectious disease diagnosis in animals","volume":"263","author":"Burrough","year":"2025","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Garc\u00eas, A., Lopes, R., Silva, A., Sampaio, F., Duque, D., and Brilhante-Sim\u00f5es, P. (2022). Bacterial isolates from urinary tract infection in dogs and cats in Portugal, and their antibiotic susceptibility pattern: A retrospective study of 5 years (2017\u20132021). Antibiotics, 11.","DOI":"10.3390\/antibiotics11111520"},{"key":"ref_33","unstructured":"Miller, W.H., Griffin, C.E., and Campbell, K.L. (2013). M\u00fcller and Kirk\u2019s Small Animal Dermatology, Elsevier Saunders. [7th ed.]."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"170","DOI":"10.2460\/javma.258.2.170","article-title":"Implementation of an antimicrobial stewardship program in a veterinary medical teaching institution","volume":"258","author":"Feyes","year":"2021","journal-title":"J. Am. Vet. Med. Assoc."}],"container-title":["Veterinary Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-7381\/13\/9\/892\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T12:03:51Z","timestamp":1788264231000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-7381\/13\/9\/892"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,31]]},"references-count":34,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["vetsci13090892"],"URL":"https:\/\/doi.org\/10.3390\/vetsci13090892","relation":{},"ISSN":["2306-7381"],"issn-type":[{"value":"2306-7381","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,31]]}}}