{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T15:29:13Z","timestamp":1788535753864,"version":"build-2803163510"},"reference-count":23,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T00:00:00Z","timestamp":1788480000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Zhaoqing University \u201cHundred\u2013Thousand Initiative\u201d","award":["bqw202601"],"award-info":[{"award-number":["bqw202601"]}]},{"award":["bqw202601"],"award-info":[{"award-number":["bqw202601"]}],"id":[{"id":"https:\/\/ror.org\/025n5kj18","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Zhaoqing Science and Technology Program","award":["241226090091099"],"award-info":[{"award-number":["241226090091099"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vet. Sci.","Veterinary Sciences"],"abstract":"<jats:p>Porcine rotavirus A (PoRVA) is an important cause of diarrhea in neonatal piglets, resulting in significant economic losses in the swine industry. In this study, a PoRVA strain, designated QY, was isolated from diarrheic piglets and systematically characterized. The virus was propagated in MA104 cells and identified by immunofluorescence assay and transmission electron microscopy, showing typical cytopathic effects and spherical particles of approximately 70 nm. Whole-genome sequencing and phylogenetic analysis revealed that QY was a G4P[13] strain with a genotype constellation of G4\u2013P[13]\u2013I1\u2013C1\u2013M1\u2013R1\u2013A8\u2013N1\u2013T1\u2013E1\u2013H1. Several genome segments showed close phylogenetic relationships with human- or bat-associated RVA strains, a pattern consistent with a possible reassortment history of QY. Experimental infection showed that QY caused watery diarrhea, intestinal lesions, and viral shedding in neonatal piglets under the experimental conditions used in this study. High viral loads were detected in small intestinal tissues, accompanied by villous atrophy and epithelial degeneration. These findings provide new insights into the genetic diversity and evolution of PoRVA and further characterize the pathogenicity of the G4P[13] QY strain in neonatal piglets.<\/jats:p>","DOI":"10.3390\/vetsci13090907","type":"journal-article","created":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T15:09:28Z","timestamp":1788534568000},"page":"907","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A G4P[13] Porcine Rotavirus a Strain with Genomic Features Suggestive of Reassortment: Isolation, Genomic Characterization, and Pathogenicity in Piglets"],"prefix":"10.3390","volume":"13","author":[{"given":"Xianyu","family":"Zhang","sequence":"first","affiliation":[{"name":"School of Food & Pharmaceutical Engineering, Zhaoqing University, Zhaoqing 526061, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"},{"role":"software","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"}]},{"given":"Rui","family":"Geng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"}]},{"given":"Shengjin","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Animal Science, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"project-administration","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"}]},{"given":"Liguo","family":"Gao","sequence":"additional","affiliation":[{"name":"College of Animal Science, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"project-administration","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"}]},{"given":"Qunhui","family":"Li","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Enterprise Key Laboratory of Healthy Animal Husbandry and Environment Control, Wen\u2019s Foodstuff Group Co., Ltd., Yunfu 527400, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"supervision","vocabulary":"credit"},{"role":"conceptualization","vocabulary":"credit"}]},{"given":"Yongchang","family":"Cao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"}]},{"given":"Yu","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Animal Science, South China Agricultural University, Guangzhou 510642, China"},{"name":"Guangdong Provincial Enterprise Key Laboratory of Healthy Animal Husbandry and Environment Control, Wen\u2019s Foodstuff Group Co., Ltd., Yunfu 527400, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"software","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7556-2268","authenticated-orcid":false,"given":"Hanqin","family":"Shen","sequence":"additional","affiliation":[{"name":"School of Food & Pharmaceutical Engineering, Zhaoqing University, Zhaoqing 526061, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"conceptualization","vocabulary":"credit"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/S0034-5288(18)33493-3","article-title":"A rotavirus in lambs with diarrhoea","volume":"20","author":"Snodgrass","year":"1976","journal-title":"Res. 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