{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T17:10:00Z","timestamp":1788801000309,"version":"build-2803163510"},"reference-count":30,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T00:00:00Z","timestamp":1788739200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vet. Sci.","Veterinary Sciences"],"abstract":"<jats:p>Vitamins and vitamin-like compounds are critical in human periodontitis pathogenesis. Limited reports on vitamin alterations in canine periodontal disease (PD) are available. A prospective, exploratory, cross-sectional pilot study evaluating plasma concentrations of vitamins and vitamin-like compounds by liquid chromatography and mass spectrometry was undertaken. The test cohort included 17 dogs with severe periodontitis, in at least two quadrants, and eight periodontally healthy control dogs seen for non-periodontal oral conditions. Strict inclusion and exclusion criteria were applied to the canine patients enrolled in this private specialty practice pilot study. After adjustment for multiple comparisons, only vitamin B9 (folate) was significantly associated with severe PD, with geometric mean concentrations approximately 28% lower in dogs with severe PD than in controls (0.00139 vs. 0.00193 \u03bcmol\/L; adjusted p = 0.006). In exploratory unadjusted analyses, several additional metabolites had lower geometric mean concentrations in dogs with severe PD. Given the small and unbalanced sample and the inability to standardize diet, these findings should be interpreted as preliminary. Further investigation in larger, prospective, diet-controlled studies integrating vitamin profiling, metabolomics, and the oral microbiome is warranted.<\/jats:p>","DOI":"10.3390\/vetsci13090916","type":"journal-article","created":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T16:30:02Z","timestamp":1788798602000},"page":"916","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Vitamins and Vitamin-like Compounds in Severe Canine Periodontal Disease: A Prospective Exploratory Cross-Sectional Pilot Study"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-1520-6397","authenticated-orcid":false,"given":"Suzanna L.","family":"Hatunen","sequence":"first","affiliation":[{"name":"Veterinary Dental Services, Boxborough, MA 01719, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6065-2516","authenticated-orcid":false,"given":"Jamie G.","family":"Anderson","sequence":"additional","affiliation":[{"name":"School of Dental Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Meng, Z., Zheng, W., Meng, X., and Xu, H. (2024). The association of composite dietary antioxidant index with periodontitis in NHANES 2009\u20132014. Front. Immunol., 15.","DOI":"10.3389\/fimmu.2024.1384272"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1111\/prd.12297","article-title":"The role of inflammation and genetics in periodontal disease","volume":"83","author":"Loos","year":"2020","journal-title":"Periodontol. 2000"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Mi, N., Zhang, M., Ying, Z., Lin, X., and Jin, Y. (2024). Vitamin intake and periodontal disease: A meta-analysis of observational studies. BMC Oral Health, 24.","DOI":"10.1186\/s12903-024-03850-5"},{"key":"ref_4","first-page":"e81694","article-title":"Micronutrients and the periodontium: A narrative review","volume":"17","author":"Doshi","year":"2025","journal-title":"Cureus"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1016\/j.jobcr.2023.05.009","article-title":"Serum levels of various vitamins in periodontal health and disease\u2014A cross sectional study","volume":"13","author":"Hans","year":"2023","journal-title":"J. Oral Biol. Craniofacial Res."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1111\/jre.13387","article-title":"The association of intake of vitamin mixtures with periodontitis: A machine learning approach on NHANES","volume":"60","author":"Zhang","year":"2025","journal-title":"J. Periodontal Res."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Li, W., Shang, Q., Yang, D., Peng, J., Zhao, H., Xu, H., and Chen, Q. (2022). Abnormal micronutrient intake is associated with the risk of periodontitis: A dose\u2013response association study based on NHANES 2009\u20132014. Nutrients, 14.","DOI":"10.3390\/nu14122466"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1007\/s00784-025-06527-7","article-title":"Association between vitamin K intake and periodontitis in American elderly population: A cross-sectional analysis of NHANES data","volume":"29","author":"Zhu","year":"2025","journal-title":"Clin. Oral Investig."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1111\/adj.12606","article-title":"Periodontal disease severity is associated with micronutrient intake","volume":"63","author":"Luo","year":"2018","journal-title":"Aust. Dent. J."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Liang, F., Lu, M., and Zhou, Y. (2024). Associations between single and multiple dietary vitamins and the risk of periodontitis: Results from NHANES 2009\u20132014. Front. Nutr., 11.","DOI":"10.3389\/fnut.2024.1347712"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.nutres.2024.12.003","article-title":"Low folate biomarker concentrations are associated with increase the risk and severity of periodontitis in adults: A cross-sectional study based on the National Health and Nutrition Examination Survey 2009\u20132014","volume":"134","author":"Zhao","year":"2025","journal-title":"Nutr. Res."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Martinon, P., Fraticelli, L., Giboreau, A., Dussart, C., Bourgeois, D., and Carrouel, F. (2021). Nutrition as a key modifiable factor for periodontitis and main chronic diseases. J. Clin. Med., 10.","DOI":"10.3390\/jcm10020197"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Cunha, E., Tavares, L., and Oliveira, M. (2022). Revisiting periodontal disease in dogs: How to manage this new old problem?. Antibiotics, 11.","DOI":"10.3390\/antibiotics11121729"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1016\/0030-4220(55)90194-8","article-title":"Oral lesions in niacin, riboflavin, pyridoxine, folic acid, and pantothenic acid deficiencies in adult dogs. Part I. Lingual lesions","volume":"8","author":"Afonsky","year":"1955","journal-title":"Oral Surg. Oral Med. Oral Pathol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"867","DOI":"10.1016\/0030-4220(55)90246-2","article-title":"Oral lesions in niacin, riboflavin, pyridoxine, folic acid, and pantothenic acid deficiencies in adult dogs. Part II. Gingival and periodontal lesions","volume":"8","author":"Afonsky","year":"1955","journal-title":"Oral Surg. Oral Med. Oral Pathol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1177\/08987564231189650","article-title":"The effect of subacute oral folic acid treatment on growth of Porphyromonas gulae in dogs","volume":"41","author":"Shirahata","year":"2024","journal-title":"J. Vet. Dent."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"8197","DOI":"10.12681\/jhvms.35300","article-title":"Serum ascorbic acid and calcidiol status in dogs with graded periodontitis","volume":"75","author":"Azargoun","year":"2025","journal-title":"J. Hell. Vet. Med. Soc."},{"key":"ref_18","unstructured":"American Veterinary Dental College (AVDC) (2026, August 19). American Veterinary Dental College Website. Available online: https:\/\/avdc.org\/avdc-nomenclature\/."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"49","DOI":"10.5326\/JAAHA-MS-6933","article-title":"2019 AAHA Dental Care Guidelines for Dogs and Cats","volume":"55","author":"Bellows","year":"2019","journal-title":"J. Am. Anim. Hosp. Assoc."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"60","DOI":"10.3102\/10769986025001060","article-title":"On the adaptive control of the false discovery rate in multiple testing with independent statistics","volume":"25","author":"Benjamini","year":"2000","journal-title":"J. Educ. Behav. Stat."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Liu, W., and Guo, D. (2025). Oxidative stress in periodontitis and the application of antioxidants in treatment: A narrative review. Front. Physiol., 16.","DOI":"10.3389\/fphys.2025.1485367"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"e17250","DOI":"10.1111\/jvim.17250","article-title":"The effects of age and sex on reference intervals for cobalamin, homocysteine, and serum and urinary methylmalonic acid in healthy adult dogs","volume":"39","author":"Proksch","year":"2025","journal-title":"J. Vet. Intern. Med."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Buzatu, R., Luca, M.M., and Bumbu, B.A. (2025). The role of vitamin B complex in periodontal disease: A systematic review examining supplementation outcomes, age differences in children and adults, and aesthetic changes. Nutrients, 17.","DOI":"10.3390\/nu17071166"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Fascetti, A.J., Delaney, S.J., Larsen, J.A., and Villaverde, C. (2023). Applied Veterinary Clinical Nutrition, Wiley.","DOI":"10.1002\/9781119375241"},{"key":"ref_25","unstructured":"Hand, M.S., Thatcher, C.D., Remillard, R.L., Roudebush, P., and Novotny, B.J. (2010). Small Animal Clinical Nutrition, Mark Morris Institute. [5th ed.]."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/j.disamonth.2018.09.009","article-title":"Nutrition and oral health","volume":"65","author":"Gondivkar","year":"2019","journal-title":"Dis. Mon."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1111\/j.1532-5415.2006.01020.x","article-title":"The association between serum folate levels and periodontal disease in older adults: Data from the National Health and Nutrition Examination Survey 2001\/02","volume":"55","author":"Yu","year":"2007","journal-title":"J. Am. Geriatr. Soc."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Ebersole, J.L., Lambert, J., Bush, H., Huja, P.E., and Basu, A. (2018). Serum nutrient levels and aging effects on periodontitis. Nutrients, 10.","DOI":"10.3390\/nu10121986"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Cagetti, M.G., Wolf, T.G., Tennert, C., Camoni, N., Lingstr\u00f6m, P., and Campus, G. (2020). The role of vitamins in oral health. A systematic review and meta-analysis. Int. J. Environ. Res. Public Health, 17.","DOI":"10.3390\/ijerph17030938"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1007\/s44371-025-00100-7","article-title":"Quantification of fat-soluble vitamins in human serum by LC\u2013MS\/MS","volume":"2","author":"Wen","year":"2025","journal-title":"Discov. 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