{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T05:07:53Z","timestamp":1788930473252,"version":"build-2803163510"},"reference-count":34,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T00:00:00Z","timestamp":1788739200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012144","name":"Hebei Agricultural University","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100012144","id-type":"DOI","asserted-by":"crossref"}]},{"id":[{"id":"https:\/\/ror.org\/009fw8j44","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vet. Sci.","Veterinary Sciences"],"abstract":"<jats:p>The objective of this study was to examine the depletion kinetics of enrofloxacin and doxycycline in quail eggs and develop suitable withdrawal times. Four hundred healthy laying Chinese White-feathered quails were randomly assigned to five groups (n = 80 each): a control group and two dose groups (high and low) for each drug. The doses were set at the maximum dose recommended for chickens and at half of that dose, according to the Chinese Veterinary Pharmacopeia. Both drugs were administered continuously for 5 days via drinking water. Eggs were collected at different time points during the administration period and after withdrawal, until residues fell below the detection limit. During the administration period, residues in quail eggs increased progressively, with the highest accumulation found in albumen. After drug withdrawal, the elimination patterns were similar across the dose levels. Residues in albumen declined more rapidly than in yolk. For both drugs, residue concentrations declined rapidly during the initial period after withdrawal and more slowly thereafter. Using the whole-egg residue data and the Chinese national standard (GB 31650.1-2022) maximum residue limits (MRLs) of 10 \u00b5g\/kg, the withdrawal times were calculated as follows: for enrofloxacin, 10.75 days (0.15 g\/L) and 9.03 days (0.075 g\/L); for doxycycline, 25.56 days (0.3 g\/L) and 21.30 days (0.15 g\/L). These results provide valuable data for residue monitoring and risk management, and offer a scientific reference for food safety assurance of quail eggs.<\/jats:p>","DOI":"10.3390\/vetsci13090917","type":"journal-article","created":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T16:30:02Z","timestamp":1788798602000},"page":"917","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Residue Depletion of Enrofloxacin and Doxycycline in Eggs of Chinese White-Feathered Quail After Administration via Drinking Water"],"prefix":"10.3390","volume":"13","author":[{"given":"Yabin","family":"Zhang","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, Hebei Agricultural University, Baoding 071000, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"software","vocabulary":"credit"}]},{"given":"Xudong","family":"Fu","sequence":"additional","affiliation":[{"name":"Shijiazhuang Livestock Product and Veterinary Drug Feed Quality Testing Center, Shijiazhuang 050000, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"}]},{"given":"Songyan","family":"Liu","sequence":"additional","affiliation":[{"name":"Shijiazhuang Livestock Product and Veterinary Drug Feed Quality Testing Center, Shijiazhuang 050000, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"}]},{"given":"Wei","family":"Lyu","sequence":"additional","affiliation":[{"name":"Shijiazhuang Luancheng District Agricultural Product Quality Monitoring Station, Shijiazhuang 050000, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"validation","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"given":"Lining","family":"He","sequence":"additional","affiliation":[{"name":"Shijiazhuang Livestock Product and Veterinary Drug Feed Quality Testing Center, Shijiazhuang 050000, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"validation","vocabulary":"credit"},{"role":"software","vocabulary":"credit"}]},{"given":"Rui","family":"Song","sequence":"additional","affiliation":[{"name":"Shijiazhuang Livestock Product and Veterinary Drug Feed Quality Testing Center, Shijiazhuang 050000, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"given":"Mengxue","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shijiazhuang Livestock Product and Veterinary Drug Feed Quality Testing Center, Shijiazhuang 050000, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"software","vocabulary":"credit"}]},{"given":"Zhiheng","family":"Wang","sequence":"additional","affiliation":[{"name":"Shijiazhuang Livestock Product and Veterinary Drug Feed Quality Testing Center, Shijiazhuang 050000, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"given":"Yun","family":"Li","sequence":"additional","affiliation":[{"name":"Shijiazhuang Livestock Product and Veterinary Drug Feed Quality Testing Center, Shijiazhuang 050000, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"}]},{"given":"Jianhua","family":"Qin","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Hebei Agricultural University, Baoding 071000, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"supervision","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,7]]},"reference":[{"key":"ref_1","first-page":"425","article-title":"Current status, existing challenges, and future development recommendations for China\u2019s quail industry","volume":"62","author":"Wang","year":"2026","journal-title":"Chin. J. Anim. Sci."},{"key":"ref_2","unstructured":"QYResearch (2026). 2026\u20132032 Global and China Quail Eggs Industry Research and 15th Five-Year Plan Analysis Report, QYResearch."},{"key":"ref_3","first-page":"331","article-title":"Comparison of egg quality characteristics of different poultry species","volume":"12","author":"Wijedasa","year":"2020","journal-title":"J. Agric. Sci."},{"key":"ref_4","first-page":"1504","article-title":"Antibiotic residues and dietary exposure risk assessment in poultry eggs sold through e-commerce channels","volume":"50","author":"Zhang","year":"2023","journal-title":"Mod. Prev. Med."},{"key":"ref_5","first-page":"132","article-title":"Residue levels and health risks of quinolones and tetracyclines in fresh eggs sold in Shanghai","volume":"55","author":"Qiu","year":"2026","journal-title":"J. Hyg. Res."},{"key":"ref_6","first-page":"3882","article-title":"Residues status of fipronil and its metabolites in eggs and \u201cminority\u201d eggs","volume":"46","author":"Yi","year":"2019","journal-title":"Mod. Prev. Med."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Ma, X., Chen, L., Yin, L., Li, Y., Yang, X., Yang, Z., Li, G., and Shan, H. (2022). Risk Analysis of 24 Residual Antibiotics in Poultry Eggs in Shandong, China (2018\u20132020). Vet. Sci., 9.","DOI":"10.3390\/vetsci9030126"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Khalifa, H.O., Shikoray, L., Mohamed, M.Y.I., Habib, I., and Matsumoto, T. (2024). Veterinary drug residues in the food chain as an emerging public health threat: Sources, analytical methods, health impacts, and preventive measures. Foods, 13.","DOI":"10.3390\/foods13111629"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Wang, Y., Wang, J., Zhang, L., Han, S., Pan, X., Wen, H., Yang, H., Wang, X., and Peng, D. (2026). Veterinary drug residues in food chains: Sources, exposure pathways, health impacts, mitigation, and safety assurance. Foods, 15.","DOI":"10.3390\/foods15050840"},{"key":"ref_10","unstructured":"Food and Drug Administration (2026, July 01). Withdrawal of Enrofloxacin for Poultry. U.S. Food and Drug Administration, Available online: https:\/\/www.fda.gov\/animal-veterinary\/recalls-withdrawals\/withdrawal-enrofloxacin-poultry."},{"key":"ref_11","first-page":"260","article-title":"Comparative analysis of pesticide and veterinary drug residue limits for food safety of poultry eggs in European Union and China","volume":"15","author":"Li","year":"2024","journal-title":"J. Food Saf. Qual."},{"key":"ref_12","unstructured":"(2019). National Food Safety Standard\u2014Maximum Residue Limits for Veterinary Drugs in Foods (Standard No. GB 31650-2019)."},{"key":"ref_13","unstructured":"(2022). National Food Safety Standard\u2014Maximum Residue Limits for 41 Veterinary Drugs in Foods (Standard No. GB 31650.1-2022)."},{"key":"ref_14","unstructured":"European Union (2026, July 09). HydroDoxx 500 mg\/g Powder for Use in Drinking Water for Chickens and Pigs [Product Information]. Veterinary Medicines Information Website. Available online: https:\/\/medicines.health.europa.eu\/veterinary\/en\/600000039498."},{"key":"ref_15","first-page":"387","article-title":"Current status, problems, and countermeasures of pigeon, quail, and special poultry industry in China","volume":"60","author":"Zhu","year":"2024","journal-title":"Chin. J. Anim. Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1080\/00071660802613286","article-title":"Comparative pharmacokinetics of florfenicol in the chicken, pigeon and quail","volume":"50","author":"Ismail","year":"2009","journal-title":"Br. Poult. Sci."},{"key":"ref_17","unstructured":"Commission of Chinese Veterinary Pharmacopoeia (2020). Veterinary Drug Quality Standards, 2020 Commission of Chinese Veterinary Pharmacopoeia."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Chen, H., Wu, X., Cui, S., Li, Y., Mu, Y., Gao, J., Liu, H., and Liu, J. (2024). Residue Elimination Patterns and Determination of the Withdrawal Times of Seven Antibiotics in Eggs of Taihang Chickens. Animals, 14.","DOI":"10.3390\/ani14243701"},{"key":"#cr-split#-ref_19.1","unstructured":"European Commission (2021). Commission Implementing Regulation"},{"key":"#cr-split#-ref_19.2","unstructured":"(EU) 2021\/808 of 22 March 2021 on the performance of analytical methods for residues of pharmacologically active substances used in food-producing animals and on the interpretation of results as well as on the methods to be used for sampling and repealing Decisions 2002\/657\/EC and 98\/179\/EC. Off. J. Eur. Union L, 180, 84-109. Available online: https:\/\/eur-lex.europa.eu\/legal-content\/EN\/TXT\/?uri=CELEX:32021R0808."},{"key":"ref_20","unstructured":"(2017). Conformity Assessment-Guidance on Validation and Verification of Chemical Analytical Methods (Standard No. GB\/T 27417-2017)."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"6397","DOI":"10.1021\/jf000145p","article-title":"Residues of veterinary drugs in eggs and their distribution between yolk and white","volume":"48","author":"Kan","year":"2000","journal-title":"J. Agric. Food Chem."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1111\/j.1365-2885.2011.01283.x","article-title":"Study of enrofloxacin and flumequine residues depletion in eggs of laying hens after oral administration","volume":"35","author":"Cornejo","year":"2012","journal-title":"J. Vet. Pharmacol. Ther."},{"key":"ref_23","first-page":"82","article-title":"Research progress on the elimination of commonly used antibiotics in chickens and their residue patterns in eggs","volume":"42","author":"Chen","year":"2020","journal-title":"China Poult."},{"key":"ref_24","first-page":"356","article-title":"Residue depletion and metabolism of enrofloxacin in eggs from laying hens after pre-laying administration","volume":"61","author":"Zhang","year":"2025","journal-title":"Chin. J. Anim. Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"101341","DOI":"10.1016\/j.psj.2021.101341","article-title":"Transfer of enrofloxacin, ciprofloxacin, and lincomycin into eggshells and residue depletion in egg components after multiple oral administration to laying hens","volume":"100","author":"Gajda","year":"2021","journal-title":"Poult. Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"101536","DOI":"10.1016\/j.psj.2021.101536","article-title":"The influence of the site of drug administration on florfenicol pharmacokinetics in turkeys","volume":"101","author":"Bello","year":"2022","journal-title":"Poult. Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1080\/23144599.2020.1765720","article-title":"Pharmacokinetics and tissue residue of enrofloxacin in healthy, Eimeria-infected broiler chickens and those pre-treated with amprolium and toltrazuril","volume":"8","author":"Atef","year":"2020","journal-title":"Int. J. Vet. Sci. Med."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2495","DOI":"10.1021\/acs.jafc.1c07480","article-title":"Risk to public health regarding doxycycline residues in chicken claws","volume":"70","author":"Gajda","year":"2022","journal-title":"J. Agric. Food Chem."},{"key":"ref_29","unstructured":"Ou, Y.H. (2020). Residue Depletion of Doxycycline Hydrochloride Soluble Powder and Tilmicosin Soluble Powder in Eggs. [Master\u2019s Thesis, Huazhong Agricultural University]."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1116","DOI":"10.1080\/19440049.2015.1041429","article-title":"Doxycycline depletion and residues in eggs after oral administration to laying hens","volume":"32","author":"Gajda","year":"2015","journal-title":"Food Addit. Contam. Part A"},{"key":"ref_31","first-page":"5624","article-title":"Metabolism of antibiotics in eggs and chicken body tissue","volume":"13","author":"Mao","year":"2022","journal-title":"J. Food Saf. Qual."},{"key":"ref_32","first-page":"16","article-title":"Effects of lighting regime on age at first egg and production performance of laying hens","volume":"23","author":"Yang","year":"2004","journal-title":"J. Tradit. Chin. Vet. Med."},{"key":"ref_33","first-page":"2915","article-title":"Effects of different feeding methods on production performance of black-feathered quail for egg production","volume":"50","author":"Zhao","year":"2011","journal-title":"Hubei Agric. Sci."}],"container-title":["Veterinary Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-7381\/13\/9\/917\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T04:24:26Z","timestamp":1788927866000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-7381\/13\/9\/917"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,7]]},"references-count":34,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["vetsci13090917"],"URL":"https:\/\/doi.org\/10.3390\/vetsci13090917","relation":{},"ISSN":["2306-7381"],"issn-type":[{"value":"2306-7381","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,7]]}}}