{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T05:08:07Z","timestamp":1789189687692,"version":"build-2803163510"},"reference-count":41,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T00:00:00Z","timestamp":1788998400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100009967","name":"Xinjiang Production and Construction Corps","doi-asserted-by":"crossref","award":["2024DA007"],"award-info":[{"award-number":["2024DA007"]}],"id":[{"id":"10.13039\/501100009967","id-type":"DOI","asserted-by":"crossref"}]},{"award":["2024DA007"],"award-info":[{"award-number":["2024DA007"]}],"id":[{"id":"https:\/\/ror.org\/03hcmxw73","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Shihezi University Technology Transfer and Promotion Program","award":["CGZH202309"],"award-info":[{"award-number":["CGZH202309"]}]},{"award":["CGZH202309"],"award-info":[{"award-number":["CGZH202309"]}],"id":[{"id":"https:\/\/ror.org\/04x0kvm78","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vet. Sci.","Veterinary Sciences"],"abstract":"<jats:p>The emergence of antimicrobial resistance in extraintestinal pathogenic Escherichia coli (ExPEC) necessitates alternative therapies for swine. Two lytic phages, vB_EcoM_S37 and vB_EcoP_S37A, were isolated from swine wastewater and characterized for morphology, stability, host range, antibacterial efficacy, and whole-genome features. Optimal multiplicities of infection were 0.01 and 0.1, respectively. Both phages remained stable at pH 4.0\u201311.0 and 40\u201360 \u00b0C. vB_EcoM_S37 lysed 43.55% (27\/62) of tested E. coli strains, with a 5-min latent period and 1173 PFU\/cell burst size, and suppressed bacterial growth for 12 h in vitro. vB_EcoP_S37A lysed 6.45% (4\/62) of strains, exhibited a 10-min latent period and 210 PFU\/cell burst size, and maintained inhibition for 8 h. Genomic analysis revealed that both phages have linear dsDNA genomes devoid of virulence or antibiotic resistance genes. vB_EcoM_S37 (173,927 bp, 37.42% GC) belongs to the genus Mosigvirus (family Straboviridae), whereas vB_EcoP_S37A (38,725 bp, 48.64% GC) represents a novel species within the genus Teseptimavirus (family Autotranscriptaviridae). These findings support both phages as stable, safe, and promising candidates for non-antibiotic biocontrol of ExPEC infections in livestock.<\/jats:p>","DOI":"10.3390\/vetsci13090943","type":"journal-article","created":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T15:54:19Z","timestamp":1789055659000},"page":"943","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Identification and Genomic Characterization of a Novel Teseptimavirus Species (vB_EcoP_S37A) and a Mosigvirus Phage (vB_EcoM_S37) Infecting Swine Extraintestinal Pathogenic Escherichia coli"],"prefix":"10.3390","volume":"13","author":[{"given":"Lu","family":"Ran","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology, Shihezi University, Shihezi 832003, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shenghui","family":"Wan","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Shihezi University, Shihezi 832003, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kexun","family":"Lian","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Shihezi University, Shihezi 832003, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Liang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Shihezi University, Shihezi 832003, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei","family":"Zheng","sequence":"additional","affiliation":[{"name":"Xinjiang Tecon Animal Husbandry Technology Co., Ltd., Changji 831399, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2649-4144","authenticated-orcid":false,"given":"Yonggang","family":"Qu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Shihezi University, Shihezi 832003, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"ame-0014-2020","DOI":"10.1128\/microbiolspec.AME-0014-2020","article-title":"Distinguishing Pathovars from Nonpathovars: Escherichia coli","volume":"8","author":"Riley","year":"2020","journal-title":"Microbiol. 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