{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:13:38Z","timestamp":1792790018884,"version":"4.5.0"},"reference-count":44,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T00:00:00Z","timestamp":1789516800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Natural Science Foundation of Sichuan Province","award":["2024NSFSC1279"],"award-info":[{"award-number":["2024NSFSC1279"]}]},{"name":"Natural Science Foundation of Sichuan Province","award":["2026NSFSC0168"],"award-info":[{"award-number":["2026NSFSC0168"]}]},{"name":"Sichuan Veterinary Medicine and Drug Innovation Group of the China Agricultural Research System","award":["SCCXTD-2026-18"],"award-info":[{"award-number":["SCCXTD-2026-18"]}],"id":[{"id":"https:\/\/ror.org\/03p5eky76","id-type":"ROR","asserted-by":"crossref"}]},{"award":["SCCXTD-2026-18"],"award-info":[{"award-number":["SCCXTD-2026-18"]}],"id":[{"id":"https:\/\/ror.org\/03p5eky76","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Fengdu County Technological Innovation and Application Development Project","award":["FDkJCX202501"],"award-info":[{"award-number":["FDkJCX202501"]}]},{"name":"Dual-Support Plan for Discipline Construction of Sichuan Agricultural University","award":["2024ZYTS012"],"award-info":[{"award-number":["2024ZYTS012"]}],"id":[{"id":"https:\/\/ror.org\/0388c3403","id-type":"ROR","asserted-by":"crossref"}]},{"award":["2024ZYTS012"],"award-info":[{"award-number":["2024ZYTS012"]}],"id":[{"id":"https:\/\/ror.org\/0388c3403","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vet. Sci.","Veterinary Sciences"],"abstract":"<jats:p>Pseudorabies virus (PRV), a member of the Alphaherpesvirinae subfamily, poses a significant economic threat to the global swine industry and has raised growing public health concerns due to its cross-species transmission potential. Given emerging variants and the fact that current vaccines are restricted to pigs, novel antiviral agents are urgently needed. The present study assessed the anti-PRV activity of isoliquiritigenin, as well as its modulatory role in innate immune pathways, using cell-based assays and a PRV-infected mouse model. The results showed that isoliquiritigenin exhibited a half-maximal inhibitory concentration (IC50) of 35.47 \u03bcM and a selective index of 3.34 in PK-15 cells, with efficacy sustained at high infectious dose. Mechanistically, it counteracted PRV-induced suppression of type I interferon responses, correlating with core genes in the cGAS\/STING pathway and enhancing IRF3 phosphorylation, while also activating the JAK\/STAT axis and increasing STAT1 phosphorylation. In vivo, treatment with isoliquiritigenin decreased mortality by 40 percentage points (from 70% to 30%), substantially suppressed viral burden in various tissues, and concomitantly activated type I interferon signaling. Collectively, these findings suggest that isoliquiritigenin potentiates host innate immunity, potentially through the cGAS\/STING and JAK\/STAT cascades, offering a novel flavonoid-based lead for anti-herpesvirus drug development.<\/jats:p>","DOI":"10.3390\/vetsci13090974","type":"journal-article","created":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T16:07:29Z","timestamp":1789574849000},"page":"974","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Isoliquiritigenin Elicits Potent Antiviral Activity Against Pseudorabies Virus Through Modulating the cGAS\/STING and JAK\/STAT Signaling Cascades"],"prefix":"10.3390","volume":"13","author":[{"given":"Hongqiao","family":"Hu","sequence":"first","affiliation":[{"name":"Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"given":"Ying","family":"Yang","sequence":"additional","affiliation":[{"name":"Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"data-curation","vocabulary":"credit"}]},{"given":"Jinbin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"}]},{"given":"Yican","family":"Wang","sequence":"additional","affiliation":[{"name":"Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"}]},{"given":"Xun","family":"Zhou","sequence":"additional","affiliation":[{"name":"Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"formal-analysis","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2772-264X","authenticated-orcid":false,"given":"Lixia","family":"Li","sequence":"additional","affiliation":[{"name":"Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"supervision","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3116-1478","authenticated-orcid":false,"given":"Yuanfeng","family":"Zou","sequence":"additional","affiliation":[{"name":"Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"validation","vocabulary":"credit"}]},{"given":"Mingyue","family":"Li","sequence":"additional","affiliation":[{"name":"Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"given":"Shujun","family":"Dai","sequence":"additional","affiliation":[{"name":"Ningxia Guiliu Benniu AB Agri Biotechnology Co., Ltd., Yinchuan 750000, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"}]},{"given":"Yifei","family":"Lang","sequence":"additional","affiliation":[{"name":"Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4102-0836","authenticated-orcid":false,"given":"Xu","family":"Song","sequence":"additional","affiliation":[{"name":"Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e01934-19","DOI":"10.1128\/JVI.01934-19","article-title":"Pseudorabies virus infection accelerates degradation of the kinesin-3 motor KIF1A","volume":"94","author":"Huang","year":"2020","journal-title":"J. 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