{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T12:29:31Z","timestamp":1790166571336,"version":"4.1.0"},"reference-count":16,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2015,4,10]],"date-time":"2015-04-10T00:00:00Z","timestamp":1428624000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007249","name":"University of Minnesota","doi-asserted-by":"publisher","award":["CVM Internal"],"award-info":[{"award-number":["CVM Internal"]}],"id":[{"id":"10.13039\/100007249","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>The characteristics of canine IL-17-producing cells are incompletely understood. Expression of mRNA encoding orthologs of IL-17 and the IL-17 receptor has been documented in tissues from dogs with arthritis, inflammatory bowel disease, and lymphoma; however, no associations have been found between IL-17 gene expression and disease phenotype in these conditions. Robust assessment of the role of IL-17-producing cells in dogs will require measuring the frequency of these cells in health and disease in balance with other lymphocyte subsets. The aim of this study was to confirm that the T-cell IL-17 response in dogs is evolutionarily conserved. Canine peripheral blood mononuclear cells were stimulated with Concanavalin A with or without polarizing cytokines. We used a canine specific IL-17 ELISA and flow cytometry to identify IL-17-producing T cells. Accumulation of intracellular IL-17 was observed in stimulated CD4 and CD8 T cells.  The addition of pro-inflammatory cytokines appeared to enhance polarization of canine CD4 T cells to the Th17 phenotype. Conversely, the addition of IL-2 in the presence of TGF-\u03b2 resulted in expansion of Treg cells. We conclude that canine IL-17-producing cells behave similarly to those from humans and mice when stimulated with mitogens and polarized with pro-inflammatory or immune regulatory cytokines.<\/jats:p>","DOI":"10.3390\/vetsci2020043","type":"journal-article","created":{"date-parts":[[2015,4,10]],"date-time":"2015-04-10T17:06:18Z","timestamp":1428685578000},"page":"43-51","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Stimulation with Concanavalin-A Induces IL-17 Production by Canine Peripheral T Cells"],"prefix":"10.3390","volume":"2","author":[{"given":"Michelle","family":"Ritt","sequence":"first","affiliation":[{"name":"Department of Veterinary Clinical Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul, MN 55108, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beth","family":"Lindborg","sequence":"additional","affiliation":[{"name":"Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, St. Paul, MN 55108, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy","family":"O'Brien","sequence":"additional","affiliation":[{"name":"Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, St. Paul, MN 55108, USA"},{"name":"Stem Cell Institute, University of Minnesota, Minneapolis, MN 55455, USA"},{"name":"Masonic Cancer Center, University of Minnesota, Minneapolis, MN 55455, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph","family":"Bisignano","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul, MN 55108, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6398-7648","authenticated-orcid":false,"given":"Jaime","family":"Modiano","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul, MN 55108, USA"},{"name":"Stem Cell Institute, University of Minnesota, Minneapolis, MN 55455, USA"},{"name":"Masonic Cancer Center, University of Minnesota, Minneapolis, MN 55455, USA"},{"name":"Center for Immunology, University of Minnesota, Minneapolis, MN 55455, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2015,4,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1093\/intimm\/dxp021","article-title":"Th17 cells: From precursors to players in inflammation and infection","volume":"21","author":"Awasthi","year":"2009","journal-title":"Int. Immunol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1016\/j.immuni.2011.03.005","article-title":"Autocrine transforming growth factor-beta1 promotes in vivo th17 cell differentiation","volume":"34","author":"Gutcher","year":"2011","journal-title":"Immunity"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Bedoya, S.K., Lam, B., Lau, K., and Larkin, J. (2013). Th17 cells in immunity and autoimmunity. Clin. Dev. Immunol.","DOI":"10.1155\/2013\/986789"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.vetimm.2012.01.013","article-title":"Gene expression of selected signature cytokines of T cell subsets in duodenal tissues of dogs with and without inflammatory bowel disease","volume":"146","author":"Schmitz","year":"2012","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Liang, Y., Pan, H.F., and Ye, D.Q. (2014). Tc17 cells in immunity and systemic autoimmunity. Int. Rev. Immunol.","DOI":"10.3109\/08830185.2014.954698"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"e1004396","DOI":"10.1371\/journal.ppat.1004396","article-title":"Mhc class II restricted innate-like double negative T cells contribute to optimal primary and secondary immunity to leishmania major","volume":"10","author":"Mou","year":"2014","journal-title":"PLoS Pathog."},{"key":"ref_7","first-page":"153","article-title":"Intestinal cytokine mrna expression in canine inflammatory bowel disease: A meta-analysis with critical appraisal","volume":"59","author":"Jergens","year":"2009","journal-title":"Comp. Med."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/j.vetimm.2013.07.006","article-title":"Expression of CD4+ T cell cytokine genes in the colorectal mucosa of inflammatory colorectal polyps in miniature dachshunds","volume":"155","author":"Ohta","year":"2013","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1177\/0300985812465325","article-title":"Molecular profiling reveals prognostically significant subtypes of canine lymphoma","volume":"50","author":"Frantz","year":"2013","journal-title":"Vet. Pathol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1046\/j.1365-2249.2000.01328.x","article-title":"Antigen-induced IL-17 response in the peripheral blood mononuclear cells (PBMC) of healthy controls","volume":"122","author":"Lenarczyk","year":"2000","journal-title":"Clin. Exp. Immunol."},{"key":"ref_11","first-page":"32","article-title":"Cluster formation of canine dendritic cells and lymphocytes is calcium dependent and not inhibited by cyclosporine","volume":"18","author":"Aprile","year":"1990","journal-title":"Exp. Hematol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1146\/annurev.immunol.021908.132710","article-title":"IL-17 and Th17 cells","volume":"27","author":"Korn","year":"2009","journal-title":"Annu. Rev. Immunol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2654","DOI":"10.1002\/eji.200838541","article-title":"Th memory for interleukin-17 expression is stable in vivo","volume":"38","author":"Lexberg","year":"2008","journal-title":"Eur. J. Immunol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0165-2427(92)90030-T","article-title":"Immunophysiological studies of interleukin-2 and canine lymphocytes","volume":"33","author":"Helfand","year":"1992","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"494","DOI":"10.1111\/j.1939-165X.2010.00252.x","article-title":"Exclusion of cytoplasmic fragments in flow cytometric analysis of lymph node samples from dogs with lymphoma using membrane-permeable violet laser-excitable DNA-binding fluorescent dye (dyecycle violet)","volume":"39","author":"Ito","year":"2010","journal-title":"Vet. Clin. Pathol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"6535","DOI":"10.4238\/2013.December.11.5","article-title":"Application and effects of mouse foxp3 antibody and fixation\/permeabilization buffer on the detection of CD4+ regulatory T cells in various mammal species","volume":"12","author":"Sun","year":"2013","journal-title":"Genet. Mol. Res."}],"container-title":["Veterinary Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-7381\/2\/2\/43\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T20:44:32Z","timestamp":1760215472000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-7381\/2\/2\/43"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,4,10]]},"references-count":16,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2015,6]]}},"alternative-id":["vetsci2020043"],"URL":"https:\/\/doi.org\/10.3390\/vetsci2020043","relation":{},"ISSN":["2306-7381"],"issn-type":[{"value":"2306-7381","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,4,10]]}}}