{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T16:43:45Z","timestamp":1784133825840,"version":"3.55.0"},"reference-count":50,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2017,9,4]],"date-time":"2017-09-04T00:00:00Z","timestamp":1504483200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>The prevalence of intestinal parasites and vector-borne agents of dogs and cats in the Pine Ridge Reservation, South Dakota were determined. Fecal samples (84 dogs, 9 cats) were examined by centrifugal floatation and by immunofluorescence assay (FA) for Giardia and Cryptosporidium. PCR was performed on Giardia [beta-giardin (bg), triose phosphate isomerase (tpi), glutamate dehydrogenase genes (gdh)] and Cryptosporidium [heat shock protein-70 gene (hsp)] FA positive samples. Cat sera (n = 32) were tested for antibodies against Bartonella spp., Toxoplasma gondii, and FIV, and antigens of FeLV and Dirofilaria immitis. Dog sera (n = 82) were tested for antibodies against T. gondii, Borrelia burgdorferi, Ehrlichia canis, and Anaplasma phagocytophilum and D. immitis antigen. Blood samples (92 dogs, 39 cats) were assessed by PCR for amplification of DNA of Bartonella spp., Ehrlichia spp., Anaplasma spp., haemoplasmas, and Babesia spp. (dogs only). The most significant results were Giardia spp. (32% by FA), Taenia spp. (17.8%) and Cryptosporidium spp. (7.1%). The Giardia isolates typed as the dog-specific assemblages C or D and four Cryptosporidium isolates typed as C. canis. Antibodies against T. gondii were detected in 15% of the dogs. Antibodies against Bartonella spp. and against T. gondii were detected in 37.5% and 6% of the cats respectively. FeLV antigen was detected in 10% of the cats.<\/jats:p>","DOI":"10.3390\/vetsci4030043","type":"journal-article","created":{"date-parts":[[2017,9,4]],"date-time":"2017-09-04T11:11:52Z","timestamp":1504523512000},"page":"43","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Prevalence of Selected Zoonotic and Vector-Borne Agents in Dogs and Cats on the Pine Ridge Reservation"],"prefix":"10.3390","volume":"4","author":[{"given":"A.","family":"Scorza","sequence":"first","affiliation":[{"name":"Center for Companion Animal Studies, Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Lappin","sequence":"additional","affiliation":[{"name":"Center for Companion Animal Studies, Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2017,9,4]]},"reference":[{"key":"ref_1","unstructured":"Glasmeier, A.K. (2006). Distressed Regions Section. An Atlas of Poverty in America: One Nation, Pulling Apart, 1960\u20132003, Routledge Taylor & Francis Group."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1016\/j.vetpar.2009.07.044","article-title":"Prevalence of intestinal parasites in pet dogs in the United States","volume":"166","author":"Little","year":"2009","journal-title":"Vet. Parasitol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"52","DOI":"10.5326\/0390052","article-title":"Prevalence of enteric pathogens in dogs of north-central Colorado","volume":"39","author":"Hackett","year":"2003","journal-title":"J. Am. Anim. Hosp. Assoc."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1016\/j.pt.2010.01.004","article-title":"Minimal zoonotic risk of cryptosporidiosis from pet dogs and cats","volume":"26","author":"Griffiths","year":"2010","journal-title":"Trends Parasitol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1053\/j.tcam.2010.07.007","article-title":"Update on the diagnosis and management of Cryptosporidium spp. infections in dogs and cats","volume":"25","author":"Scorza","year":"2010","journal-title":"Top. Companion Anim. Med."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1111\/j.1939-1676.2007.tb02974.x","article-title":"Genotypic analysis of Giardia duodenalis in domestic cats","volume":"21","author":"Vasilopulos","year":"2007","journal-title":"J. Vet. Intern. Med."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.exppara.2009.01.003","article-title":"Cryptosporidiosis and giardiasis in dogs and cats: Veterinary and public health importance","volume":"124","author":"Bowman","year":"2010","journal-title":"Exp. Parasitol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"179","DOI":"10.5326\/JAAHA-MS-5598","article-title":"Dirofilaria immitis infection in dogs from underserved, Native American reservations in the United States","volume":"47","author":"Theis","year":"2011","journal-title":"J. Am. Anim. Hosp. Assoc."},{"key":"ref_9","unstructured":"(2017, August 25). Climate Porcupine, South Dakota. Available online: http:\/\/www.usclimatedata.com\/climate\/porcupine\/south-dakota\/united-states\/ussd0274."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/S0304-4017(00)00235-1","article-title":"Prevalence and genotypic characterization of Giardia in dairy calves from Western Australia and Western Canada","volume":"90","author":"Olson","year":"2000","journal-title":"Vet. Parasitol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/S1084-8592(99)80050-2","article-title":"Fast and reliable extraction of protozoan parasite dna from fecal specimens","volume":"4","author":"Moura","year":"1999","journal-title":"Mol. Diagn."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1016\/S0020-7519(00)00164-8","article-title":"Molecular and Phylogenetic characterization of Cryptosporidium from birds","volume":"31","author":"Morgan","year":"2001","journal-title":"Int. J. Parasitol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/j.meegid.2004.02.001","article-title":"Discrimination of all genotypes of Giardia duodenalis at the glutamate dehydrogenase locus using PCR-RFLP","volume":"4","author":"Read","year":"2004","journal-title":"Infect. Genet. Evol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1645\/GE-293R","article-title":"Genotyping of Giardia duodenalis from humans and dogs from Mexico using a beta-giardin nested polymerase chain reaction assay","volume":"91","author":"Lalle","year":"2005","journal-title":"J. Parasitol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1444","DOI":"10.3201\/eid0911.030084","article-title":"Triosephosphate isomerase gene characterization and potential zoonotic transmission of Giardia duodenalis","volume":"9","author":"Sulaiman","year":"2003","journal-title":"Emerg. Infect. Dis."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/j.vetpar.2009.11.003","article-title":"From mouse to moose: Multilocus genotyping of Giardia isolates from various animal species","volume":"168","author":"Lebbad","year":"2010","journal-title":"Vet. Parasitol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1586","DOI":"10.2460\/ajvr.1989.50.09.1586","article-title":"Enzyme-linked immunosorbent assay for the detection of circulating antigens of Toxoplasma gondii in the serum of cats","volume":"50","author":"Lappin","year":"1989","journal-title":"Am. J. Vet. Res."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.jfms.2008.06.005","article-title":"Prevalence of Bartonella species antibodies and Bartonella species DNA in the blood of cats with and without fever","volume":"11","author":"Lappin","year":"2009","journal-title":"J. Feline Med. Surg."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1717","DOI":"10.1128\/JCM.38.5.1717-1722.2000","article-title":"Rapid identification and differentiation of Bartonella spp. using a single-step PCR assay","volume":"38","author":"Jensen","year":"2000","journal-title":"J. Clin. Microbiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"604","DOI":"10.2460\/ajvr.2001.62.604","article-title":"Use of a polymerase chain reaction assay to detect and differentiate two strains of Haemobartonella felis in naturally infected cats","volume":"62","author":"Jensen","year":"2001","journal-title":"Am. J. Vet. Res."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/j.vetmic.2004.06.013","article-title":"Pathogen carriage by the cat flea C. felis (Buoch\u00e9) in the United Kingdom","volume":"102","author":"Shaw","year":"2004","journal-title":"Vet. Microbiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"893","DOI":"10.2460\/javma.2004.225.893","article-title":"Molecular and serological evidence of Anaplasma phagocytophilum infection of cats in North America","volume":"225","author":"Lappin","year":"2004","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"4172","DOI":"10.1128\/JCM.41.9.4172-4177.2003","article-title":"Development and Evaluation of a Seminested PCR for Detection and Differentiation of Babesia gibsoni (Asian Genotype) and B. canis DNA in Canine Blood Samples","volume":"41","author":"Birkenheuer","year":"2003","journal-title":"J. Clin. Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1016\/j.vetpar.2012.04.032","article-title":"Comparisons of mammalian Giardia duodenalis assemblages based on the \u03b2-giardin, glutamate dehydrogenase and triose phosphate isomerase genes","volume":"189","author":"Scorza","year":"2012","journal-title":"Vet. Parasitol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1007\/s00436-013-3666-y","article-title":"Characterization of Giardia lamblia genotypes in dogs from Tucson, Arizona using SSU-rRNA and \u03b2-giardin sequences","volume":"113","author":"Johansen","year":"2014","journal-title":"Parasitol. Res."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"959","DOI":"10.1111\/j.1939-1676.2007.tb03049.x","article-title":"Comparison of direct immunofluorescence, immunoassays, and fecal flotation for detection of Cryptosporidium spp. and Giardia spp. in naturally exposed cats in 4 Northern California animal shelters","volume":"21","author":"Mekaru","year":"2007","journal-title":"J. Vet. Int. Med."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1128\/CMR.00033-10","article-title":"Zoonotic potential and molecular epidemiology of Giardia species and giardiasis","volume":"24","author":"Feng","year":"2011","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1089\/vbz.2011.0751","article-title":"Genotyping Giardia duodenalis isolates from dogs: Lessons from a multilocus sequence typing study","volume":"12","author":"Beck","year":"2012","journal-title":"Vector Borne Zoonotic Dis."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1186\/1756-3305-7-412","article-title":"Low risk for transmission of zoonotic Giardia duodenalis from dogs to humans in rural Cambodia","volume":"7","author":"Inpankaew","year":"2014","journal-title":"Parasit. Vectors"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1963","DOI":"10.1007\/s00436-015-4385-3","article-title":"Copromicroscopic and molecular investigations on intestinal parasites in kenneled dogs","volume":"114","author":"Simonato","year":"2015","journal-title":"Parasitol. Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1186\/s13071-014-0615-2","article-title":"Thompson, R.C. The epidemiology of infections with Giardia species and genotypes in well cared for dogs and cats in Germany","volume":"8","author":"Pallant","year":"2015","journal-title":"Parasit. Vectors"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.vetpar.2010.11.029","article-title":"Genotypic characterization of Giardia from domestic dogs in the USA","volume":"177","author":"Covacin","year":"2011","journal-title":"Vet. Parasitol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.vetpar.2004.09.008","article-title":"The zoonotic significance and molecular epidemiology of Giardia and giardiasis","volume":"126","author":"Thompson","year":"2004","journal-title":"Vet. Parasitol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1016\/j.vetpar.2011.08.019","article-title":"Prevalence of Giardia and Cryptosporidium species in dog park attending dogs compared to non-dog park attending dogs in one region of Colorado","volume":"184","author":"Wang","year":"2012","journal-title":"Vet. Parasitol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1239","DOI":"10.1016\/j.ijpara.2008.03.006","article-title":"Molecular characterization of species and genotypes of Cryptosporidium and Giardia and assessment of zoonotic transmission","volume":"38","author":"Xiao","year":"2008","journal-title":"Int. J. Parasitol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1009","DOI":"10.1016\/j.cvsm.2009.08.001","article-title":"Toxoplasmosis","volume":"39","author":"Dubey","year":"2009","journal-title":"Vet. Clin. North Am. Small Anim. Pract."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"750","DOI":"10.1645\/GE-1496R.1","article-title":"Toxoplasma gondii and Neospora caninum antibodies in dogs from Grenada, West Indies","volume":"94","author":"Dubey","year":"2008","journal-title":"J. Parasitol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"e76","DOI":"10.1111\/j.1863-2378.2010.01332.x","article-title":"Toxoplasma gondii and Trypanosoma cruzi antibodies in dogs from Virginia","volume":"57","author":"Rosypal","year":"2010","journal-title":"Zoonoses Public Health"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1016\/j.parint.2016.05.001","article-title":"Risk factors of Toxoplasma gondii infection in hunting, pet and watchdogs from southern Spain and northern Africa","volume":"65","author":"Dubey","year":"2016","journal-title":"Parasitol. Int."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1627","DOI":"10.1007\/s00436-015-4900-6","article-title":"Toxoplasmosis in the Caribbean islands: Literature review, seroprevalence in pregnant women in ten countries, isolation of viable Toxoplasma gondii from dogs from St. Kitts, West Indies with report of new T. gondii genetic types","volume":"115","author":"Dubey","year":"2016","journal-title":"Parasitol. Res."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/S0304-4017(97)00048-4","article-title":"Mechanical transmission of Toxoplasma gondii oocysts by dogs","volume":"73","author":"Lindsay","year":"1997","journal-title":"Vet. Parasitol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1016\/S1201-9712(03)90112-3","article-title":"Dogs as possible mechanical carriers of Toxoplasma, and their fur as a source of infection of young children","volume":"7","author":"Frenkel","year":"2003","journal-title":"Int. J. Infect. Dis."},{"key":"ref_43","first-page":"421","article-title":"Enteric parasites of Indians and Anglo-Americans, chiefly on the Winnebago and Omaha reservations in Nebraska","volume":"53","author":"Becker","year":"1968","journal-title":"Nebr. State Med. J."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1003","DOI":"10.1542\/peds.65.5.1003","article-title":"Pulmonary echinococcosis: A pediatric disease of the Southwestern United States","volume":"65","author":"Katz","year":"1980","journal-title":"Pediatrics"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Hotez, P.J. (2008). Neglected Infections of Poverty in the United States of America. PLoS Negl. Trop. Dis., 2.","DOI":"10.1371\/journal.pntd.0000256"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.actatropica.2012.02.066","article-title":"Challenges for diagnosis and control of cystic hydatid disease","volume":"123","author":"Barnesa","year":"2012","journal-title":"Acta Trop."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1145","DOI":"10.1093\/infdis\/172.4.1145","article-title":"Prevalence of Bartonella henselae antibodies in pet cats throughout regions of North America","volume":"172","author":"Jameson","year":"1995","journal-title":"J. Infect. Dis."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"2445","DOI":"10.1128\/jcm.33.9.2445-2450.1995","article-title":"Bartonella henselae prevalence in domestic cats in California: Risk factors and association between bacteremia and antibody titers","volume":"33","author":"Chomel","year":"1995","journal-title":"J. Clin. Microbiol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1016\/j.vetpar.2008.10.093","article-title":"Prevalence and geographic distribution of Dirofilaria immitis, Borrelia burgdorferi, Erlichia canis, and Ana plasma phagocytophilum in dogs in the United States: Results of a national clinic-based serologic survey","volume":"160","author":"Bowman","year":"2009","journal-title":"Vet. Parasitol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/j.jfms.2003.11.005","article-title":"Prevalence of infectious diseases in feral cats in Northern Florida","volume":"6","author":"Luria","year":"2004","journal-title":"J. Feline Med. Surg."}],"container-title":["Veterinary Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-7381\/4\/3\/43\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T18:44:03Z","timestamp":1760208243000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-7381\/4\/3\/43"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,9,4]]},"references-count":50,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2017,9]]}},"alternative-id":["vetsci4030043"],"URL":"https:\/\/doi.org\/10.3390\/vetsci4030043","relation":{},"ISSN":["2306-7381"],"issn-type":[{"value":"2306-7381","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,9,4]]}}}