{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T22:59:02Z","timestamp":1767826742220,"version":"3.49.0"},"reference-count":32,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2017,12,1]],"date-time":"2017-12-01T00:00:00Z","timestamp":1512086400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>Animal leptospirosis, exempt in rodents, manifests as peculiar biology where the animal can function, simultaneously or not, as a susceptible host or reservoir. In the first case, clinical symptoms are likely. In the second case, infection is subclinical and manifestations are mild or absent. Mild clinical symptoms encompass reproductive failure in production animals for host-adapted Leptospira sp. serovars. This work presents a study on Leptospira sp. infection in a mixed-species (bovine and swine) farm with documented reproductive disorders in the cattle unit. A long calving interval (above 450 days) was the hallmark observed in cows. Some cows (2\/26 tested) presented a high titre of antibodies against Leptospira sp. serogroup Sejroe, but the overall within-herd prevalence was low (11.5% and 7.7% for cut-off titres of 1:30 and 1:100, respectively). The in-herd prevalence of leptospirosis in the sow unit (determined for 113\/140 animals) was high when using a lowered cut-off threshold (32.7% vs. 1.8% for cut-off titre of 1:30 and 1:100, respectively). In this unit, the most prevalent serogroup was Autumnalis. The final diagnostic confirmation of Leptospira sp. maintenance within the farm was obtained through detection by PCR of Leptospira sp. DNA in an aborted swine litter. Despite the fact that a common causative infective agent was diagnosed in both species, the direct link between the two animal units was not found. Factors such as drinking from the same water source and the use of manure prepared with the swine slurry might raise suspicion of a possible cross-contamination between the two units. In conclusion, this work suggests that leptospirosis be included in the differential diagnosis of reproductive disorders and spontaneous abortions in production animals and provides data that justify the use of a lowered threshold cut-off for herd diagnosis.<\/jats:p>","DOI":"10.3390\/vetsci4040064","type":"journal-article","created":{"date-parts":[[2017,12,1]],"date-time":"2017-12-01T12:30:16Z","timestamp":1512131416000},"page":"64","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Reproductive Disorders and Leptospirosis: A Case Study in a Mixed-Species Farm (Cattle and Swine)"],"prefix":"10.3390","volume":"4","author":[{"given":"Marcella","family":"Mori","sequence":"first","affiliation":[{"name":"Bacterial Zoonoses of Livestock, Operational Directorate Bacterial Diseases, Veterinary and Agrochemical Research Centre, CODA-CERVA, 1180 Brussels, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ra\u00efssa","family":"Bakinahe","sequence":"additional","affiliation":[{"name":"Bacterial Zoonoses of Livestock, Operational Directorate Bacterial Diseases, Veterinary and Agrochemical Research Centre, CODA-CERVA, 1180 Brussels, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philippe","family":"Vannoorenberghe","sequence":"additional","affiliation":[{"name":"Bacterial Zoonoses of Livestock, Operational Directorate Bacterial Diseases, Veterinary and Agrochemical Research Centre, CODA-CERVA, 1180 Brussels, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jo","family":"Maris","sequence":"additional","affiliation":[{"name":"Dierengezondheidszorg Vlaanderen, DGZ, 8820 Torhout, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ellen","family":"De Jong","sequence":"additional","affiliation":[{"name":"Dierengezondheidszorg Vlaanderen, DGZ, 8820 Torhout, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maryl\u00e8ne","family":"Tignon","sequence":"additional","affiliation":[{"name":"Virology, Operational Directorate Enzotic and (re)emerging diseases, Veterinary and Agrochemical Research Centre, CODA-CERVA, 1180 Brussels, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martine","family":"Marin","sequence":"additional","affiliation":[{"name":"Bacterial Zoonoses of Livestock, Operational Directorate Bacterial Diseases, Veterinary and Agrochemical Research Centre, CODA-CERVA, 1180 Brussels, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Damien","family":"Desqueper","sequence":"additional","affiliation":[{"name":"Bacterial Zoonoses of Livestock, Operational Directorate Bacterial Diseases, Veterinary and Agrochemical Research Centre, CODA-CERVA, 1180 Brussels, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Fretin","sequence":"additional","affiliation":[{"name":"Bacterial Zoonoses of Livestock, Operational Directorate Bacterial Diseases, Veterinary and Agrochemical Research Centre, CODA-CERVA, 1180 Brussels, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isabelle","family":"Behaeghel","sequence":"additional","affiliation":[{"name":"Epidemiology, Operational Directorate Interaction and surveillance, Veterinary and Agrochemical Research Centre, CODA-CERVA, 1180 Brussels, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2017,12,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1016\/j.mimet.2012.06.005","article-title":"Comparison of conventional PCR, quantitative PCR, bacteriological culture and the Warthin Starry technique to detect Leptospira spp. in kidney and liver samples from naturally infected sheep from Brazil","volume":"90","author":"Fornazari","year":"2012","journal-title":"J. Microbiol. Methods"},{"key":"ref_2","first-page":"132","article-title":"Establishment of Valid Laboratory Case Definition for Human Leptospirosis","volume":"3","author":"Goris","year":"2012","journal-title":"J. Bacteriol. Parasitol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.vetmic.2013.01.032","article-title":"Asymptomatic and chronic carriage of Leptospira interrogans serovar Pomona in California sea lions (Zalophus californianus)","volume":"164","author":"Prager","year":"2013","journal-title":"Vet. Microbiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1007\/s11259-013-9582-x","article-title":"Urinary PCR as an increasingly useful tool for an accurate diagnosis of leptospirosis in livestock","volume":"38","author":"Hamond","year":"2014","journal-title":"Vet. Res. Commun."},{"key":"ref_5","unstructured":"OIE (2014). Leptospirosis. OIE Terrestrial Manual 2014, OIE. Chapter 2.1.9."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"792","DOI":"10.1354\/vp.08-VP-0265-N-REV","article-title":"Review paper: Host-pathogen interactions in the kidney during chronic leptospirosis","volume":"46","author":"Monahan","year":"2009","journal-title":"Vet. Pathol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"756","DOI":"10.4269\/ajtmh.13-0416","article-title":"Distribution of Leptospira serogroups in cattle herds and dogs in France","volume":"91","author":"Ayral","year":"2014","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/j.diagmicrobio.2009.03.014","article-title":"Detection of pathogenic Leptospira spp. through TaqMan polymerase chain reaction targeting the LipL32 gene","volume":"64","author":"Stoddard","year":"2009","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.jviromet.2005.07.004","article-title":"A broadly reactive one-step real-time RT-PCR assay for rapid and sensitive detection of hepatitis E virus","volume":"131","author":"Jothikumar","year":"2006","journal-title":"J. Virol. Methods"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1016\/j.rvsc.2007.10.003","article-title":"Simultaneous detection and genotyping of porcine reproductive and respiratory syndrome virus (PRRSV) by real-time RT-PCR and amplicon melting curve analysis using SYBR Green","volume":"85","author":"Riera","year":"2008","journal-title":"Res. Vet. Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1136\/vr.139.5.110","article-title":"Reduced conception rates in dairy cattle associated with serological evidence of Leptospira interrogans serovar hardjo infection","volume":"139","author":"Dhaliwal","year":"1996","journal-title":"Vet. Rec."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1021","DOI":"10.1016\/j.theriogenology.2005.08.028","article-title":"Influence of leptospirosis on reproductive performance of sows in Brazil","volume":"66","author":"Ramos","year":"2006","journal-title":"Theriogenology"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1136\/vr.138.12.272","article-title":"Reproductive performance of dairy herds infected with Leptospira interrogans serovar hardjo relative to the year of diagnosis","volume":"138","author":"Dhaliwal","year":"1996","journal-title":"Vet. Rec."},{"key":"ref_14","first-page":"99","article-title":"Animal leptospirosis","volume":"387","author":"Ellis","year":"2015","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1136\/vr.117.5.101","article-title":"Bovine leptospirosis: Some clinical features of serovar hardjo infection","volume":"117","author":"Ellis","year":"1985","journal-title":"Vet. Rec."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1136\/vr.110.8.178","article-title":"Bovine leptospirosis: Serological findings in aborting cows","volume":"110","author":"Ellis","year":"1982","journal-title":"Vet. Rec."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1111\/j.1751-0813.1989.tb09719.x","article-title":"Leptospira interrogans serovar hardjo is not a major cause of bovine abortion in Victoria","volume":"66","author":"Chappel","year":"1989","journal-title":"Aust. Vet. J."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/j.vetmic.2009.03.012","article-title":"Leptospira and leptospirosis","volume":"140","author":"Adler","year":"2010","journal-title":"Vet. Microbiol."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Rocha, B.R., Narduche, L., Oliveira, C.S., Martins, G., and Lilenbaum1, W. (2017). Molecular demonstration of intermittent shedding of Leptospira in cattle and sheep and its implications on control. Cienc. Rural, 47.","DOI":"10.1590\/0103-8478cr20170088"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1239","DOI":"10.4315\/0362-028X.JFP-17-016","article-title":"Antibodies to Various Zoonotic Pathogens Detected in Feral Swine (Sus scrofa) at Abattoirs in Texas, USA","volume":"80","author":"Pedersen","year":"2017","journal-title":"J. Food Prot."},{"key":"ref_21","first-page":"129","article-title":"Serological surveillance of Leptospirosis in Italy: Two-year national data (2010\u20132011)","volume":"52","author":"Tagliabue","year":"2016","journal-title":"Vet. Ital."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1054","DOI":"10.3855\/jidc.5507","article-title":"Characterization of Leptospira interrogans serovar Pomona isolated from swine in Brazil","volume":"9","author":"Miraglia","year":"2015","journal-title":"J. Infect. Dev. Ctries."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1007\/s11250-016-1201-8","article-title":"A systematic review of leptospirosis on dogs, pigs, and horses in Latin America","volume":"49","author":"Pinto","year":"2017","journal-title":"Trop. Anim. Health Prod."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1007\/s11250-013-0508-y","article-title":"Leptospirosis in pigs, dogs, rodents, humans, and water in an area of the Colombian tropics","volume":"46","author":"Calderon","year":"2014","journal-title":"Trop. Anim. Health Prod."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Lee, H.S., Khong, N.V., Xuan, H.N., Nghia, V.B., Nguyen-Viet, H., and Grace, D. (2017). Sero-prevalence of specific Leptospira serovars in fattening pigs from 5 provinces in Vietnam. BMC Vet. Res., 13.","DOI":"10.1186\/s12917-017-1044-1"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"699","DOI":"10.20506\/rst.17.3.1128","article-title":"A review of leptospirosis in farm animals in Portugal","volume":"17","author":"Rocha","year":"1998","journal-title":"Rev. Sci. Tech."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"913","DOI":"10.20506\/rst.35.3.2579","article-title":"Leptospirosis in domestic animals in France: Serological results from 1988 to 2007","volume":"35","year":"2016","journal-title":"Rev. Sci. Tech."},{"key":"ref_28","first-page":"133","article-title":"The occurrence of antibodies to various Leptospira serotypes in swine in The Netherlands in the period 1975\u20131980","volume":"108","author":"Bercovich","year":"1983","journal-title":"Tijdschr. Diergeneeskd."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1086\/597036","article-title":"Resurgence of field fever in a temperate country: An epidemic of leptospirosis among seasonal strawberry harvesters in Germany in 2007","volume":"48","author":"Desai","year":"2009","journal-title":"Clin. Infect. Dis."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1016\/j.trstmh.2010.07.002","article-title":"Climate change, flooding, urbanisation and leptospirosis: Fuelling the fire?","volume":"104","author":"Lau","year":"2010","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Ledien, J., Sorn, S., Hem, S., Huy, R., Buchy, P., Tarantola, A., and Cappelle, J. (2017). Assessing the performance of remotely-sensed flooding indicators and their potential contribution to early warning for leptospirosis in Cambodia. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0181044"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1158","DOI":"10.15585\/mmwr.mm6642a9","article-title":"Notes from the Field: Postflooding Leptospirosis\u2014Louisiana, 2016","volume":"66","author":"Frawley","year":"2017","journal-title":"MMWR Morb. Mortal Wkly. Rep."}],"container-title":["Veterinary Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-7381\/4\/4\/64\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T18:52:06Z","timestamp":1760208726000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-7381\/4\/4\/64"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,12,1]]},"references-count":32,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2017,12]]}},"alternative-id":["vetsci4040064"],"URL":"https:\/\/doi.org\/10.3390\/vetsci4040064","relation":{},"ISSN":["2306-7381"],"issn-type":[{"value":"2306-7381","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,12,1]]}}}