{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T23:20:44Z","timestamp":1780096844807,"version":"3.54.0"},"reference-count":40,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2018,10,16]],"date-time":"2018-10-16T00:00:00Z","timestamp":1539648000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>The anthelminthic efficacy of the crude extracts of Hypoestes forskaolii (Vahl) R.Br (Acanthaceae) against gastrointestinal nematodes (GIN) in sheep was investigated using the in vitro egg hatch inhibition assay. Faecal samples were collected from sheep with naturally occurring infection of GIN (Trichostrongylus spp., Chabertia ovina, Cooperia spp., Haemonchus contortus and Teladorsagia spp.). Crude leaf extracts of H. forskaolii was obtained using increasing polarity solvents: n-hexane, chloroform, chloroform:methanol 9:1, methanol. Thiabendazole (0.2 \u00b5g\/mL and 0.5 \u00b5g\/mL) was used as a positive control and untreated GIN eggs in deionised water served as the negative control. All the extracts exhibited a weak ovicidal activity against GIN (less than 50% of egg hatch). Noteworthy, the n-hexane extract showed a percentage of inhibition of egg hatching greater than other extracts inhibiting the 30.8% at the concentration of 1 mg\/mL showing a dose-dependent effect on nematode eggs hatching. Further studies are needed to investigate the effects of extracts used and to evaluate the ovicidal effects of other extracts of H. forskaolii.<\/jats:p>","DOI":"10.3390\/vetsci5040089","type":"journal-article","created":{"date-parts":[[2018,10,16]],"date-time":"2018-10-16T02:52:53Z","timestamp":1539658373000},"page":"89","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["In Vitro Anthelminthic Efficacy of Hypoestes forskaolii (Vahl) R.Br (Acanthaceae) Extracts on Gastrointestinal Nematodes of Sheep"],"prefix":"10.3390","volume":"5","author":[{"given":"Massimiliano","family":"D\u2019Ambola","sequence":"first","affiliation":[{"name":"Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, 80137 Naples, Italy"},{"name":"Department of Pharmacy, University of Salerno, 84084 Fisciano, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Bosco","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, 80137 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Ariano","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, 80137 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Rinaldi","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, 80137 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2201-7201","authenticated-orcid":false,"given":"Ammar","family":"Bader","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy, Umm Al-Qura University, 21955 Makkah, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandra","family":"Amadesi","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, 80137 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe","family":"Cringoli","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, 80137 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorella","family":"Severino","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, 80137 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2018,10,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"884","DOI":"10.1016\/j.tvjl.2013.03.025","article-title":"Co-infection by Escherichia coli O157 and gastrointestinal strongyles in sheep","volume":"197","author":"Dipineto","year":"2013","journal-title":"Vet. J."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"325","DOI":"10.4081\/gh.2015.355","article-title":"Haemonchus contortus: Spatial risk distribution for infection in sheep in Europe","volume":"9","author":"Rinaldi","year":"2015","journal-title":"Geospat. Health"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"257","DOI":"10.4081\/gh.2015.347","article-title":"Mapping and modelling helminth infections in ruminants in Europe: Experience from GLOWORM","volume":"9","author":"Rinaldi","year":"2015","journal-title":"Geospat. Health"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1016\/j.pt.2014.04.009","article-title":"Chasing helminths and their economic impact on farmed ruminants","volume":"30","author":"Charlier","year":"2014","journal-title":"Trends Parasitol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"2566","DOI":"10.1007\/s11356-014-3497-0","article-title":"Toxicity of anthelmintic drugs (fenbendazole and flubendazole) to aquatic organisms","volume":"22","author":"Wagil","year":"2015","journal-title":"Environ. Sci. Pollut. Res. Int."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"279","DOI":"10.4314\/ajtcam.v7i4.56143","article-title":"Anthelminthic efficacy of aqueous extract of Acanthus montanus leaf against strongylid nematodes of small ruminants","volume":"7","author":"Adamu","year":"2010","journal-title":"Afr. J. Tradit. Complement. Altern. Med."},{"key":"ref_7","unstructured":"Githiori, J.B. (2004). Evaluation of Anthelmintic Properties of Ethnoveterinary Plant Preparations Used as Livestock Dewormers by Pastoralists and Small holder Farmers in Kenya. [Ph.D. Thesis, Swedish University of Agricultural Sciences]."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"428","DOI":"10.1016\/j.jep.2006.10.003","article-title":"In vitro and in vivo anthelmintic activity of crude extracts of Coriandrum sativum against Haemonchus contortus","volume":"110","author":"Eguale","year":"2007","journal-title":"J. Ethnopharmacol."},{"key":"ref_9","unstructured":"Harborne, A.J. (1998). Phytochemical Methods a Guide to Modern Techniques of Plant Analysis, Springer."},{"key":"ref_10","first-page":"129","article-title":"Toxicity of Crepis vesicaria L. in ruminants: Preliminary results","volume":"32","author":"Russo","year":"2009","journal-title":"J. Vet. Pharmacol. Ther."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/S0921-4488(00)00163-2","article-title":"Anthelmintic activity of medicinal plants with particular reference to their use in animals in the Indo\u2013Pakistan subcontinent","volume":"38","author":"Akhtar","year":"2000","journal-title":"Small Rumin. Res."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1023\/A:1013310809275","article-title":"Towards the scientific validation of traditional medicinal plants","volume":"34","author":"Taylor","year":"2001","journal-title":"J. Plant. Growth Regul."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/j.vetpar.2008.07.021","article-title":"Anthelmintic efficacy and dose determination of Albizia anthelmintica against gastrointestinal nematodes in naturally infected Ugandan sheep","volume":"157","author":"Arble","year":"2008","journal-title":"Vet. Parasitol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1023\/A:1005884429253","article-title":"Prospects for plant anthelmintics in tropical veterinary medicine","volume":"21","author":"Hammond","year":"1997","journal-title":"Vet. Res. Commun."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1007\/s11418-011-0589-8","article-title":"In vitro cytotoxic screening of selected Saudi medicinal plants","volume":"66","author":"Almehdar","year":"2012","journal-title":"J. Nat. Med."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1947","DOI":"10.1021\/np0497402","article-title":"Furanolabdane diterpenes from Hypoestes purpurea","volume":"67","author":"Shen","year":"2004","journal-title":"J. Nat. Prod."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"14349","DOI":"10.3390\/molecules171214349","article-title":"Assessment of the in vitro antiprotozoal and cytotoxic potential of 20 selected medicinal plants from the island of Soqotra","volume":"17","author":"Mothana","year":"2012","journal-title":"Molecules"},{"key":"ref_18","first-page":"550","article-title":"Plants of the mountains of Sarat and Hejaz","volume":"2","author":"Ghushash","year":"2006","journal-title":"Sarawat Press"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/S0254-6299(16)31685-4","article-title":"Taxonomic studies in the Acanthaceae; The genus Hypoestes in southern Africa","volume":"51","author":"Balkwill","year":"1985","journal-title":"S. Afr. J. Bot."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1016\/j.jep.2005.06.022","article-title":"Anthelmintic activity of Calotropis procera (Ait.) Ait. F. flowers in sheep","volume":"102","author":"Iqbal","year":"2005","journal-title":"J. Ethnopharmacol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1186\/1472-6882-13-66","article-title":"Extracts of Euphorbia hirta Linn. (Euphorbiaceae) and Rauvolfia vomitoria Afzel (Apocynaceae) demonstrate activities against Onchocerca volvulus microfilariae in vitro","volume":"13","author":"Attah","year":"2013","journal-title":"MC Complement. Altern. Med."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1080\/13880200802315618","article-title":"In vitro anthelmintic effects of medicinal plants used in Czech Republic","volume":"46","author":"Urban","year":"2008","journal-title":"Pharm. Biol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.vetpar.2014.02.006","article-title":"The maintenance of anthelmintic efficacy in sheep in a Mediterranean climate","volume":"203","author":"Rinaldi","year":"2014","journal-title":"Vet. Parasitol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/j.vetpar.2005.11.019","article-title":"The detection of anthelmintic resistance in nematodes of veterinary importance","volume":"136","author":"Coles","year":"2006","journal-title":"Vet. Parasitol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1016\/j.vetpar.2014.12.029","article-title":"A comparison of the FECPAK and Mini-FLOTAC faecal egg counting techniques","volume":"207","author":"Godber","year":"2015","journal-title":"Vet. Parasitol."},{"key":"ref_26","first-page":"305","article-title":"Prevalence of GIT helminths in cattle at the vicinities of tehsil Jatoi, Punjab, Pakistan","volume":"25","author":"Raza","year":"2013","journal-title":"Sci. Int. Lahore"},{"key":"ref_27","first-page":"1","article-title":"Morphological identification of parasitic nematode infective larvae of small ruminants and cattle: A practical lab guide","volume":"80","author":"Mayhew","year":"2013","journal-title":"Onderstepoort J. Vet. Res."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/S0304-4017(03)00007-4","article-title":"Efficacy of eprinomectin pour-on against gastrointestinal nematode infections in sheep","volume":"112","author":"Cringoli","year":"2003","journal-title":"Vet. Parasitol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/0304-4017(92)90141-U","article-title":"World Association for the Advancement of Veterinary Parasitology (WAAVP) methods for the detection of anthelmintic resistance in nematodes of veterinary importance","volume":"44","author":"Coles","year":"1992","journal-title":"Vet. Parasitol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"825","DOI":"10.1007\/s00436-009-1466-1","article-title":"Standardization of the egg hatch test for the detection of benzimidazole resistance in parasitic nematodes","volume":"105","author":"Coles","year":"2009","journal-title":"Parasitol. Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.vetpar.2011.05.033","article-title":"Challenges of nematode control in ruminants: Focus on Latin America","volume":"180","author":"Molento","year":"2011","journal-title":"Vet. Parasitol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.smallrumres.2011.10.012","article-title":"Managing anthelmintic resistance in goats\u2014Can we learn lessons from sheep?","volume":"103","author":"Jackson","year":"2012","journal-title":"Small Rumin. Res."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1590\/S1516-05722009000300016","article-title":"Plant efficacy in small ruminant gastrointestinal nematode control: A review of published studies","volume":"11","author":"Nery","year":"2009","journal-title":"Rev. Bras. Pl. Med."},{"key":"ref_34","first-page":"466","article-title":"A review on anthelmintic plants","volume":"7","author":"Mali","year":"2008","journal-title":"Nat. Prod. Rad."},{"key":"ref_35","first-page":"79","article-title":"Ovicidal and larvicidal properties of Adhatoda vasica (L.) extracts against gastrointestinal nematodes of sheep in vitro","volume":"28","author":"Phulan","year":"2008","journal-title":"Pak. Vet. J."},{"key":"ref_36","first-page":"1","article-title":"A review of traditional uses, phytochemicals and bioactivities of the Genus","volume":"15","author":"Al","year":"2018","journal-title":"Hypoestes. Afr. J. Tradit. Complement. Altern. Med."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.phytol.2014.06.020","article-title":"Hypoestenonols A and B, new fusicoccane diterpenes from Hypoestes forskalei","volume":"10","author":"Mothana","year":"2014","journal-title":"Phytochem. Lett."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.vetpar.2003.07.021","article-title":"Ovicidal and larvicidal activity in vitro of Spigelia anthelmia Linn. extracts on Haemonchus contortus","volume":"117","author":"Assis","year":"2003","journal-title":"Vet. Parasitol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1016\/j.vetpar.2006.03.007","article-title":"Ovicidal and larvicidal activity of Melia azedarach extracts on Haemonchus contortus","volume":"140","author":"Maciel","year":"2006","journal-title":"Vet. Parasitol."},{"key":"ref_40","first-page":"57","article-title":"Anthelmintic activity of plants: A review","volume":"8","author":"Rajeswari","year":"2014","journal-title":"Res. J. Phys."}],"container-title":["Veterinary Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-7381\/5\/4\/89\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T15:25:50Z","timestamp":1760196350000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-7381\/5\/4\/89"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,10,16]]},"references-count":40,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2018,12]]}},"alternative-id":["vetsci5040089"],"URL":"https:\/\/doi.org\/10.3390\/vetsci5040089","relation":{},"ISSN":["2306-7381"],"issn-type":[{"value":"2306-7381","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,10,16]]}}}