{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T09:40:52Z","timestamp":1785577252056,"version":"3.56.0"},"reference-count":35,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2020,4,30]],"date-time":"2020-04-30T00:00:00Z","timestamp":1588204800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>Several studies in human and equine medicine have produced controversial results regarding the role of dimethylsulfoxide (DMSO) as a therapeutic agent. This study aimed to evaluate the effect of joint lavage with different DMSO concentrations on biomarkers of synovial fluid inflammation and cartilage degradation in joints with lipopolysaccharide (LPS)-induced synovitis. Twenty-six tibiotarsal joints of 13 horses were randomly distributed into four groups (lactated Ringer\u2019s solution; 5% DMSO in lactated Ringer\u2019s; 10% DMSO in lactated Ringer\u2019s; and sham). All animals were evaluated for the presence of lameness, and synovial fluid analyses were performed at 0 h, 1 h, 8 h, 24 h, and 48 h (T0, T1, T8, T24, and T48, respectively). The white blood cell counts (WBC), total protein (TP), urea, prostaglandin E2 (PGE2), interleukin (IL)-1\u03b2, IL-6, IL-10, tumor necrosis factor-\u03b1 (TNF-\u03b1), hyaluronic acid (HA), and chondroitin sulfate (CS) concentrations were measured. The WBC counts and PGE2, IL-1\u03b2, IL-6, and TP concentrations increased in all groups at T8 compared to baseline values (p &lt; 0.05). At T48, only the 5% DMSO and 10% DMSO groups showed a significant decrease in WBC counts (p &lt; 0.05). Furthermore, the 10% DMSO group had lower concentrations of PGE2 and IL-1\u03b2 at T48 than at T8 (p &lt; 0.05) and presented lower IL-6 levels than the5% DMSO and lactated Ringer\u2019s groups at T24. All groups showed an increase in CS concentration after LPS-induced synovitis. Joint lavage with 10% DMSO in lactated Ringer\u2019s has anti-inflammatory but not chondroprotective effects.<\/jats:p>","DOI":"10.3390\/vetsci7020057","type":"journal-article","created":{"date-parts":[[2020,5,4]],"date-time":"2020-05-04T03:29:39Z","timestamp":1588562979000},"page":"57","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Effects of Joint Lavage with Dimethylsulfoxide on LPS-Induced Synovitis in Horses\u2014Clinical and Laboratorial Aspects"],"prefix":"10.3390","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1938-4521","authenticated-orcid":false,"given":"Eric","family":"Sotelo","sequence":"first","affiliation":[{"name":"Departamento de Cl\u00ednica M\u00e9dica, Faculdade de Medicina Veterin\u00e1ria e Zootecnia, Universidade de S\u00e3o Paulo, S\u00e3o Paulo 05508-270, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cynthia","family":"Vendruscolo","sequence":"additional","affiliation":[{"name":"Departamento de Cl\u00ednica M\u00e9dica, Faculdade de Medicina Veterin\u00e1ria e Zootecnia, Universidade de S\u00e3o Paulo, S\u00e3o Paulo 05508-270, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joice","family":"F\u00fclber","sequence":"additional","affiliation":[{"name":"Departamento de Cl\u00ednica M\u00e9dica, Faculdade de Medicina Veterin\u00e1ria e Zootecnia, Universidade de S\u00e3o Paulo, S\u00e3o Paulo 05508-270, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0808-6887","authenticated-orcid":false,"given":"Sarah","family":"Seidel","sequence":"additional","affiliation":[{"name":"Departamento de Cl\u00ednica M\u00e9dica, Faculdade de Medicina Veterin\u00e1ria e Zootecnia, Universidade de S\u00e3o Paulo, S\u00e3o Paulo 05508-270, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando","family":"Jaramillo","sequence":"additional","affiliation":[{"name":"Departamento de Cl\u00ednica M\u00e9dica, Faculdade de Medicina Veterin\u00e1ria e Zootecnia, Universidade de S\u00e3o Paulo, S\u00e3o Paulo 05508-270, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernanda","family":"Agreste","sequence":"additional","affiliation":[{"name":"Departamento de Cl\u00ednica M\u00e9dica, Faculdade de Medicina Veterin\u00e1ria e Zootecnia, Universidade de S\u00e3o Paulo, S\u00e3o Paulo 05508-270, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu\u00eds","family":"Silva","sequence":"additional","affiliation":[{"name":"Departamento de Cirurgia, Faculdade de Medicina Veterin\u00e1ria e Zootecnia, Universidade de S\u00e3o Paulo, S\u00e3o Paulo 05508-270, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9730-0840","authenticated-orcid":false,"given":"Raquel","family":"Baccarin","sequence":"additional","affiliation":[{"name":"Departamento de Cl\u00ednica M\u00e9dica, Faculdade de Medicina Veterin\u00e1ria e Zootecnia, Universidade de S\u00e3o Paulo, S\u00e3o Paulo 05508-270, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,4,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1111\/j.1749-6632.1967.tb34865.x","article-title":"Radioprotective and cryoprotective properties of dimethyl sulfoxide in cellular systems","volume":"141","year":"1967","journal-title":"Ann. N.Y. Acad. Sci."},{"key":"ref_2","first-page":"61","article-title":"Dimethyl sulfoxide (DMSO): A review","volume":"76","author":"Brayton","year":"1986","journal-title":"Cornell Vet."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"506","DOI":"10.1111\/j.1749-6632.1967.tb34919.x","article-title":"Clinical experiences with the topical application of dmso in orthopedic diseases: Evaluation of 4180 cases","volume":"141","author":"John","year":"1967","journal-title":"Ann. N.Y. Acad. Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1035","DOI":"10.1016\/S0006-2952(03)00002-9","article-title":"Multidisciplinary utilization of dimethyl sulfoxide: Pharmacological, cellular, and molecular aspects","volume":"65","author":"Santos","year":"2003","journal-title":"Biochem. Pharmacol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s10753-006-9012-0","article-title":"Effects of resveratrol in inflammatory arthritis","volume":"30","author":"Elmali","year":"2006","journal-title":"Inflammation"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"159","DOI":"10.15210\/sah.v3i2.6585","article-title":"Toxicidade e efici\u00eancia do dimetilsulf\u00f3xido (dmso) no congelamento de c\u00e9lulas madin-darby bovine kidney (mdbk)","volume":"3","author":"Picoli","year":"2015","journal-title":"Sci. Anim. Health"},{"key":"ref_7","first-page":"187","article-title":"Evaluation of dmso transport in human articular cartilage: Vehicle solutions and effects on cell function","volume":"36","author":"Kay","year":"2015","journal-title":"Cryo Lett."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Elisia, I., Nakamura, H., Lam, V., Hofs, E., Cederberg, R., Cait, J., Hughes, M.R., Lee, L., Jia, W., and Adomat, H.H. (2016). DMSO represses inflammatory cytokine production from human blood cells and reduces autoimmune arthritis. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0152538"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1302\/2046-3758.111.2000132","article-title":"The horse as a model of naturally occurring osteoarthritis","volume":"1","author":"McIlwraith","year":"2013","journal-title":"Bone Jt. Res."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.mrfmmm.2010.06.008","article-title":"Influence of DMSO on Carbon K ultrasoft X-rays induced chromosome aberrations in V79 Chinese hamster cells","volume":"691","author":"Natarajan","year":"2010","journal-title":"Mutat. Res. Fundam. Mol. Mech. Mutagenes."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1642","DOI":"10.1172\/JCI109626","article-title":"Generation of Hydroxyl Radical by Enzymes, Chemicals, and Human Phagocytes In Vitro","volume":"64","author":"Repine","year":"2008","journal-title":"J. Clin. Investig."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1016\/j.phrs.2008.04.004","article-title":"New insights of dimethyl sulphoxide effects (DMSO) on experimental in vivo models of nociception and inflammation","volume":"57","author":"Colucci","year":"2008","journal-title":"Pharmacol Res."},{"key":"ref_13","first-page":"1180","article-title":"Evaluation of the effects of intra-articular injection of dimethylsulfoxide on normal equine articular tissues","volume":"50","author":"Welch","year":"1989","journal-title":"Am. J. Vet. Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"934","DOI":"10.2460\/ajvr.1991.52.06.934","article-title":"Effects of intra-articular administration of dimethylsulfoxide on chemically induced synovitis in immature horses","volume":"52","author":"Welch","year":"1991","journal-title":"Am. J. Vet. Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"333","DOI":"10.2460\/ajvr.1991.52.02.333","article-title":"Evaluation of use of dimethyl sulfoxide for intra-articular lavage in clinically normal horses","volume":"52","author":"Adair","year":"1991","journal-title":"Am. J. Vet. Res."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1149","DOI":"10.2460\/ajvr.1998.59.09.1149","article-title":"Anti-inflammatory effects of topically applied dimethyl sulfoxide gel on endotoxin-induced synovitis in horses","volume":"59","author":"Smith","year":"1998","journal-title":"Am. J. Vet. Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1111\/j.1751-0813.1991.tb03112.x","article-title":"Anti-inflammatory drugs inhibit degradation of equine synovial fluid induced by free radicals","volume":"68","author":"Auer","year":"1991","journal-title":"Aust. Vet. J."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1053\/jvet.2000.5607","article-title":"In vitro evaluation of the effect of dimethyl sulfoxide on equine articular cartilage matrix metabolism","volume":"29","author":"Smith","year":"2000","journal-title":"Vet. Surg."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"54","DOI":"10.2460\/ajvr.2001.62.54","article-title":"Effects of anti-inflammatory drugs on lipopolysaccharide-challenged and -unchallenged equine synovial explants","volume":"62","author":"Moses","year":"2001","journal-title":"Am. J. Vet. Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"545","DOI":"10.2746\/095777307X248641","article-title":"Evidence for dimethyl sulphoxide (DMSO) use in horses. Part 1: DMSO as a topical and intra-articular anti-inflammatory agent","volume":"19","author":"Schleining","year":"2007","journal-title":"Equine Vet. Educ."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"598","DOI":"10.2746\/095777307X255869","article-title":"Evidence for dimethyl sulphoxide (DMSO) use in horses. Part 2: DMSO as a parenteral anti-inflammatory agent and as a pharmacological carrier","volume":"19","author":"Schleining","year":"2007","journal-title":"Equine Vet. Educ."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Silva, M.M., Hagen, S.C.F., Vendruscolo, C.D.P., Baccarin, R.Y.A., Spagnolo, J.D., Yamada, A.L.M., Stievani, F., and Correia da Silva, L.C.L. (2019). The correlation between score-based protocol for equine joint assessment and subsequent arthroscopic intervention outcomes. Braz. J. Vet. Res. Anim. Sci.","DOI":"10.11606\/issn.1678-4456.bjvras.2019.158072"},{"key":"ref_23","first-page":"50","article-title":"Relevance of synovial fluid chondroitin sulphate as a biomarker to monitor polo pony joints","volume":"78","author":"Baccarin","year":"2014","journal-title":"Can. J. Vet. Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1111\/j.2042-3306.2011.00539.x","article-title":"Synovial fluid chondroitin sulphate indicates abnormal joint metabolism in asymptomatic osteochondritic horses","volume":"44","author":"Machado","year":"2012","journal-title":"Equine Vet. J."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"492","DOI":"10.1111\/j.2042-3306.1994.tb04056.x","article-title":"Experimentally-induced synovitis as a model for acute synovitis in the horse","volume":"26","author":"Palmer","year":"1994","journal-title":"Equine Vet. J."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Neuenschwander, H.M., Moreira, J.J., Vendruscolo, C.P., F\u00fclber, J., Seidel, S.R.T., Michelacci, Y.M., and Baccarin, R.Y.A. (2019). Hyaluronic acid has chondroprotective and joint-preserving effects on LPS-induced synovitis in horses. J. Vet. Sci.","DOI":"10.4142\/jvs.2019.20.e67"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/j.ejpb.2018.05.001","article-title":"Sustained intra-articular release of celecoxib in an equine repeated LPS synovitis model","volume":"128","author":"Cokelaere","year":"2018","journal-title":"Eur. J. Pharm. Biopharm."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"652","DOI":"10.1111\/j.2042-3306.2012.00571.x","article-title":"Comparison of an inertial sensor system of lameness quantification with subjective lameness evaluation","volume":"44","author":"Mccracken","year":"2012","journal-title":"Equine Vet. J."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/S0195-5616(02)00083-9","article-title":"Laboratory evaluation and interpretation of synovial fluid","volume":"33","author":"MacWilliams","year":"2003","journal-title":"Vet. Clin. North Am. Small Anim.Pract."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"379","DOI":"10.2460\/ajvr.1993.54.03.379","article-title":"Effects of intra-articularly administered endotoxin on clinical signs of disease and synovial fluid tumor necrosis factor, interleukin 6, and prostaglandin E2 values in horses","volume":"54","author":"Hawkins","year":"1993","journal-title":"Am. J. Vet. Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"866","DOI":"10.2460\/ajvr.1996.57.06.866","article-title":"Effects of pretreatment with ketoprofen and phenylbutazone on experimentally induced synovitis in horses","volume":"57","author":"Owens","year":"1996","journal-title":"Am. J. Vet. Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"6195","DOI":"10.4049\/jimmunol.174.10.6195","article-title":"Mechanisms of Dimethyl Sulfoxide Augmentation of IL-1\u03b2 Production","volume":"174","author":"Xing","year":"2005","journal-title":"J. Immunol."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"De Abreu Costa, L., Ottoni, M.H.F., Dos Santos, M.G., Meireles, A.B., De Almeida, V.G., De F\u00e1tima Pereira, W., Alves de Avelar-Freitas, B., and Eustaquio Alvim Brito-Melo, G. (2017). Dimethyl sulfoxide (DMSO) decreases cell proliferation and TNF-\u03b1, IFN-, and IL-2 cytokines production in cultures of peripheral blood lymphocytes. Molecules, 22.","DOI":"10.3390\/molecules22111789"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12917-016-0873-7","article-title":"Pro-inflammatory cytokines and structural biomarkers are effective to categorize osteoarthritis phenotype and progression in Standardbred racehorses over five years of racing career","volume":"12","author":"Bertuglia","year":"2016","journal-title":"BMC Vet. Res."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Cohen, S.B.A., Katsikis, P.D., Chu, C.Q., Thomssen, H., Webb, L.M.C., Maini, R.N., Londei, M., and Feldmann, M. (1995). High level of interleukin-10 production by the activated t cell population within the rheumatoid synovial membrane. Arthritis Rheum.","DOI":"10.1002\/art.1780380710"}],"container-title":["Veterinary Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-7381\/7\/2\/57\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T13:25:02Z","timestamp":1760361902000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-7381\/7\/2\/57"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,4,30]]},"references-count":35,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2020,6]]}},"alternative-id":["vetsci7020057"],"URL":"https:\/\/doi.org\/10.3390\/vetsci7020057","relation":{},"ISSN":["2306-7381"],"issn-type":[{"value":"2306-7381","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,4,30]]}}}