{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T20:41:30Z","timestamp":1780778490474,"version":"3.54.1"},"reference-count":24,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2020,5,1]],"date-time":"2020-05-01T00:00:00Z","timestamp":1588291200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>The Japanese black cattle breed (Wagyu) has an improved metabolism, which allows them to have a higher marbling score when compared with other cattle breeds. However, this may affect other aspects of the animal\u2019s physiology, including hormone secretion and their reproductive success, such as their response to synchronization protocols and embryo production. Therefore, the objectives of this study were to test a superovulation protocol (SOV) developed with low doses of FSH and to evaluate the outcome and economic viability of embryo production using the SOV and in vitro fertilization (IVF) approaches in the Wagyu cattle breed. For that, ten Wagyu cows were submitted to five SOVs over a period of 15 months using a standard protocol: CIDR + 3 mg estradiol benzoate (D0), 35 mg FSH (Folltropin\u00ae) a.m. and p.m. (D4), 35 mg Folltropin\u00ae a.m. and 20 mg p.m. (D5), 20 mg Folltropin\u00ae a.m. and 10 mg p.m. (D6), 10 mg Folltropin\u00ae and 0.5 mg cloprostenol, both a.m. and p.m., + CIDR removal (D7), 0.05 mg GnRH + insemination 12 and 24 h after (D8) and embryo collection + 0.5 mg of cloprostenol (D16). Thirty days after each SOV, a follicular aspiration was conducted to produce IVF embryos without any pre-synchronization using standard semen in the same group of animals. The average number of embryos produced was 7.63 \u00b1 5.61 (SOV) and 4.52 \u00b1 2.44 (IVF) (p = 0.303). There was no significant correlation between the number of embryos produced by the different techniques (SOV and IVF), indicating that cows that respond well to SOV did not respond well to IVF and vice versa (r = 0.379, p = 0.529). The total cost of each embryo produced by SOV was R$215.00 and R$410.00 for IVF. Therefore, cows that produce less than five embryos by SOV are not economically viable due their lack of response to FSH, and the use of IVF in those animals may be more effective.<\/jats:p>","DOI":"10.3390\/vetsci7020058","type":"journal-article","created":{"date-parts":[[2020,5,4]],"date-time":"2020-05-04T03:29:39Z","timestamp":1588562979000},"page":"58","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["The Outcome and Economic Viability of Embryo Production Using IVF and SOV Techniques in the Wagyu Breed of Cattle"],"prefix":"10.3390","volume":"7","author":[{"given":"Fernanda","family":"Facioli","sequence":"first","affiliation":[{"name":"Faculdade de Agronomia e Medicina Veterin\u00e1ria, Curso de Medicina Veterin\u00e1ria, Universidade de Passo Fundo, Passo Fundo, RS 99052-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fl\u00e1via","family":"De Marchi","sequence":"additional","affiliation":[{"name":"Faculdade de Agronomia e Medicina Veterin\u00e1ria, Curso de Medicina Veterin\u00e1ria, Universidade de Passo Fundo, Passo Fundo, RS 99052-900, Brazil"},{"name":"Programa de Mestrado em BioExperimenta\u00e7\u00e3o, Universidade de Passo Fundo, Passo Fundo, RS 99052-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana","family":"Marques","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Produ\u00e7\u00e3o e Sanidade Animal, Instituto Federal Catarinense, Conc\u00f3rdia, SC 89703-720, Brazil"},{"name":"Laborat\u00f3rio de Sanidade Animal, Embrapa Su\u00ednos e Aves, Conc\u00f3rdia, SC 89715-899, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulo","family":"Michelon","sequence":"additional","affiliation":[{"name":"M\u00e9dico Veterin\u00e1rio Aut\u00f4nomo, Passo Fundo, RS 99052-321, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eraldo","family":"Zanella","sequence":"additional","affiliation":[{"name":"Faculdade de Agronomia e Medicina Veterin\u00e1ria, Curso de Medicina Veterin\u00e1ria, Universidade de Passo Fundo, Passo Fundo, RS 99052-900, Brazil"},{"name":"Programa de Mestrado em BioExperimenta\u00e7\u00e3o, Universidade de Passo Fundo, Passo Fundo, RS 99052-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyle","family":"Caires","sequence":"additional","affiliation":[{"name":"Department of Human Nutrition, Food and Animal Sciences, University of Hawaii, Manoa, HI 96822, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jerry","family":"Reeves","sequence":"additional","affiliation":[{"name":"Bar-R Cattle Company, Pullman, WA 99163, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1449-6708","authenticated-orcid":false,"given":"Ricardo","family":"Zanella","sequence":"additional","affiliation":[{"name":"Faculdade de Agronomia e Medicina Veterin\u00e1ria, Curso de Medicina Veterin\u00e1ria, Universidade de Passo Fundo, Passo Fundo, RS 99052-900, Brazil"},{"name":"Programa de Mestrado em BioExperimenta\u00e7\u00e3o, Universidade de Passo Fundo, Passo Fundo, RS 99052-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,5,1]]},"reference":[{"key":"ref_1","unstructured":"AWA (2019, July 10). WHAT is Wagyu? American Wagyu Association\u2014AWA. Available online: https:\/\/wagyu.org\/breed-info\/what-is-wagyu."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"933","DOI":"10.5713\/ajas.18.0333","article-title":"The Japanese Wagyu beef industry: Current situation and future prospects\u2014A review","volume":"31","author":"Gotoh","year":"2018","journal-title":"Asian Australas. J. Anim. Sci."},{"key":"ref_3","first-page":"532","article-title":"Wagyu\u2014Japanese black beef cattle","volume":"65","author":"Siemieniuch","year":"2009","journal-title":"Med. Weter"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/jbg.12073","article-title":"Marbled Japanese Black cattle","volume":"131","author":"Hirooka","year":"2014","journal-title":"J. Anim. Breed. Genet."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"709","DOI":"10.5851\/kosfa.2016.36.6.709","article-title":"Characteristics and Health Benefit of Highly Marbled Wagyu and Hanwoo Beef","volume":"36","author":"Gotoh","year":"2016","journal-title":"Korean J. Food Sci. Anim. Resour."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1016\/j.meatsci.2009.01.026","article-title":"Differences in muscle and fat accretion in Japanese Black and European cattle","volume":"82","author":"Gotoh","year":"2009","journal-title":"Meat Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2938","DOI":"10.2527\/jas.2012-5892","article-title":"Enhancement of adipogenesis and fibrogenesis in skeletal muscle of Wagyu compared with Angus cattle","volume":"91","author":"Duarte","year":"2013","journal-title":"J. Anim. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1015","DOI":"10.2527\/jas.2014-7923","article-title":"Enhanced mitogenesis in stromal vascular cells derived from subcutaneous adipose tissue of Wagyu compared with those of Angus cattle","volume":"93","author":"Wei","year":"2015","journal-title":"J. Anim. Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1017\/S0007114509991516","article-title":"Hamburger high in total, saturated and trans-fatty acids decreases HDL cholesterol and LDL particle diameter, and increases plasma TAG in mildly hypercholesterolaemic men","volume":"103","author":"Adams","year":"2010","journal-title":"Br. J. Nutr."},{"key":"ref_10","first-page":"650","article-title":"Health implications of beef intramuscular fat consumption","volume":"36","author":"Troy","year":"2016","journal-title":"Korean J. Food Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1188","DOI":"10.3945\/jn.110.136085","article-title":"Consumption of high-oleic acid ground beef increases HDL cholesterol concentration but both high- and low-oleic acid ground beef decrease HDL particle diameter in normocholesterolemic men","volume":"141","author":"Gilmore","year":"2011","journal-title":"J. Nutr."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1790","DOI":"10.1161\/CIRCULATIONAHA.114.011687","article-title":"Dietary fat supply to failing hearts determineds dynamic lipid signaling for nuclear receptor activation and oxidation of stored triglyceride","volume":"130","author":"Lahey","year":"2014","journal-title":"Circulation"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1111\/jbg.12066","article-title":"Estimation of inbreeding and effective population size of full-blood wagyu cattle registered with the American Wagyu Cattle Association","volume":"131","author":"Scraggs","year":"2013","journal-title":"J. Anim. Breed. Genet."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Sugimoto, M., Sasaki, S., Watanabe, T., Nishimura, S., Ideta, A., Yamazaki, M., Matsuda, K., Yuzaki, M., Sakimura, K., and Aoyagi, Y. (2010). Ionotropic glutamate receptor AMPA 1 is associated with ovulation rate. PLoS ONE, 5.","DOI":"10.1371\/journal.pone.0013817"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1292\/jvms.18-0537","article-title":"Effects of Genetic Background on Responses to Superovulation in Japanese Black Cattle","volume":"81","author":"Hirayama","year":"2019","journal-title":"J. Vet. Med. Sci."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Zanella, R., Lago, L.V., Silva, A.N., Pertille, F., Silva, N.C., Panetto, J.C.C., Zanella, G.C., Facioli, F.L., and Silva, M.V.G.B. (2018). Genetic Characterization of Indubrasil Cattle Breed Population. Vet. Sci., 5.","DOI":"10.3390\/vetsci5040098"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.theriogenology.2013.09.020","article-title":"Historical perspectives and recent research on superovulation in cattle","volume":"81","author":"Mapletoft","year":"2014","journal-title":"Theriogenology"},{"key":"ref_18","first-page":"374","article-title":"Sexed Semen: Artificial Insemination and Embryo Transfer","volume":"31","author":"Baruselli","year":"2007","journal-title":"Rev. Bras. Reprod. Anim."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"2021","DOI":"10.2527\/2005.8392021x","article-title":"Genetic and economic evaluation of Japanese Black (Wagyu) cattle breeding schemes","volume":"83","author":"Kahi","year":"2005","journal-title":"J. Anim. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1111\/rda.12674","article-title":"Successful Vitrification of In Vivo Embryos Collected from Superovulated Japanese Black Cattle (Wagyu)","volume":"51","author":"An","year":"2016","journal-title":"Reprod. Domest. Anim."},{"key":"ref_21","unstructured":"(2019, November 10). MUB Beef Perform. Available online: https:\/\/www.mubbrasil.com.br\/produtos-3\/mub-beef\/mub-beef-perform."},{"key":"ref_22","unstructured":"Wright, J.M. (2010). Manual of the International Embryo Transfer Society, International Embryo Transfer Society. [4th ed.]."},{"key":"ref_23","first-page":"383","article-title":"In Vitro and In Vivo embryo production in cattle superstimulated with FSH for 7 days","volume":"12","author":"Mapletoft","year":"2015","journal-title":"Anim. Reprod."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1071\/RDv27n1Ab143","article-title":"Japanese Black (Wagyu) cattle multiple-ovulation embryo transfer: Follicle-stimulating hormone source in a case study in south China","volume":"27","author":"Du","year":"2015","journal-title":"Reprod. Fertil. Dev."}],"container-title":["Veterinary Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-7381\/7\/2\/58\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T14:09:47Z","timestamp":1760364587000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-7381\/7\/2\/58"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,5,1]]},"references-count":24,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2020,6]]}},"alternative-id":["vetsci7020058"],"URL":"https:\/\/doi.org\/10.3390\/vetsci7020058","relation":{},"ISSN":["2306-7381"],"issn-type":[{"value":"2306-7381","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,5,1]]}}}