{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T12:59:46Z","timestamp":1791291586267,"version":"4.3.0"},"reference-count":29,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2020,8,3]],"date-time":"2020-08-03T00:00:00Z","timestamp":1596412800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>Neoplastic diseases are rarely described in wild carnivores; only a few reports have been published on this topic. Here, we describe the histological and immunohistochemical features of a haemangioma in the spleen of a grey wolf (Canis lupus lupus) and we compare the results with the dog (Canis lupus familiaris). Additionally, we list the different publications found in the literature with neoplastic lesions in wolves. Our results show similar immunohistochemical features to dogs, in which neoplastic cells express Vimentin, von Willebrand factor, alpha smooth muscle actin antibody, vascular endothelial growth factor C and low vascular endothelial growth factor receptor 3. Toluidine blue special stain shows moderated increased numbers of mast cells infiltrating the tumor, a feature observed in benign vascular tumors in domestic dogs, but not in the malignant counterparts. To our knowledge, this is the first article describing the gross, histological and immunohistochemical features of a splenic haemangioma in a wolf.<\/jats:p>","DOI":"10.3390\/vetsci7030102","type":"journal-article","created":{"date-parts":[[2020,8,3]],"date-time":"2020-08-03T09:02:48Z","timestamp":1596445368000},"page":"102","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Morphological and Immunohistochemical Description of a Splenic Haemangioma in a Captive European Wolf (Canis lupus lupus) and a Review of the Current Literature"],"prefix":"10.3390","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2574-0780","authenticated-orcid":false,"given":"Josep Maria Monn\u00e9","family":"Rodr\u00edguez","sequence":"first","affiliation":[{"name":"Institute of Veterinary Pathology, Vetsuisse Faculty, University of Zurich, Winterthurerstrasse 268, CH-8057 Z\u00fcrich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8515-4452","authenticated-orcid":false,"given":"Federico","family":"Morandi","sequence":"additional","affiliation":[{"name":"National Park \u201cMonti Sibillini\u201d P.zza del Forno, 1 62039 Visso, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paolo","family":"Cavicchio","sequence":"additional","affiliation":[{"name":"Pistoia Zoological Garden, Via Pieve a Celle Nuova, 160\/A, 51100 Pistoia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1254-5772","authenticated-orcid":false,"given":"Alessandro","family":"Poli","sequence":"additional","affiliation":[{"name":"Department of Veterinary Science, University of Pisa, 56124 Viale delle Piagge, 2 56124 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ranieri","family":"Verin","sequence":"additional","affiliation":[{"name":"Department of Comparative Biomedicine and Food Science\u2014University of Padova AGRIPOLIS\u2014Viale dell\u2019 Universit\u00e0 16, 35020 Legnaro, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,8,3]]},"reference":[{"key":"ref_1","unstructured":"Maxie, M.G. (2015). Cardiovascular system. Jubb, Kennedy, and Palmer\u2019s Pathology of Domestic Animals, Elsevier. [6th ed.]."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1638\/1042-7260(2000)031[0002:SONRIC]2.0.CO;2","article-title":"Survey of necropsy results in captive red wolves (Canis rufus), 1992\u20131996","volume":"31","author":"Acton","year":"2000","journal-title":"J. Zoo Wildl. Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1016\/j.jcpa.2008.10.006","article-title":"An immunohistochemical analysis of canine haemangioma and haemangiosarcoma","volume":"140","author":"Sabattini","year":"2009","journal-title":"J. Comp. Pathol."},{"key":"ref_4","unstructured":"Meuten, D.J. (2020). Mesenchymal tumors of the skin and soft tissues. Tumors in Domestic Animals, Wiley Blackwell. [5th ed.]."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1177\/030098588802500401","article-title":"Factor VIII-related antigen in canine endothelial neoplasms: An immunohistochemical study","volume":"25","author":"Suter","year":"1988","journal-title":"Vet. Pathol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1472","DOI":"10.3923\/javaa.2010.1472.1475","article-title":"Case report of malignant mammary neoplasia in mexican gray wolf (Canis lupus baileyi)","volume":"9","year":"2010","journal-title":"J. Anim. Vet. Adv."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1136\/vr.161.12.429","article-title":"Subcutaneous leiomyosarcoma in a captive European wolf (Canis lupus)","volume":"161","author":"Bock","year":"2007","journal-title":"Vet. Rec."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"538","DOI":"10.1638\/04-019.1","article-title":"Tp53 expressing squamous cell carcinoma of the tonsil in a captive polar wolf (Canis lupus arctos)","volume":"36","author":"Teifke","year":"2005","journal-title":"J. Zoo Wildl. Med."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"165","DOI":"10.7589\/0090-3558-14.2.165","article-title":"Oral papillomatosis in coyotes (Canis latrans) and wolves (Canis lupus) of Alberta","volume":"14","author":"Samuel","year":"1978","journal-title":"J. Wildl. Dis."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1638\/2014-0198.1","article-title":"A survey of diseases in captive red wolves (Canis Rufus), 1997\u20132012","volume":"47","author":"Seeley","year":"2016","journal-title":"J. Zoo Wildl. Med."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"588","DOI":"10.1292\/jvms.16-0490","article-title":"Pulmonary neuroendocrine tumor in a female wolf (Canis lupus lupus)","volume":"79","author":"Shiraki","year":"2017","journal-title":"J. Vet. Med. Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"177","DOI":"10.7589\/2015-04-102","article-title":"Novel papillomaviral sequence detected within epidermal plaques in a wolf (Canis lupus)","volume":"52","author":"Rothenburger","year":"2016","journal-title":"J. Wildl. Dis."},{"key":"ref_13","first-page":"56","article-title":"Canine hemangiosarcoma: Retrospective analysis of 104 cases","volume":"186","author":"Brown","year":"1985","journal-title":"J. Am. Vet. Med Assoc."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1016\/S0195-5616(03)00002-0","article-title":"Hemangiosarcoma in dogs and cats: Vet","volume":"33","author":"Smith","year":"2003","journal-title":"Clin. Nord Am: Small Anim. Pract."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1111\/j.1748-5827.1976.tb06937.x","article-title":"Malignant hemangioendothelioma (angiosarcoma) in the dog","volume":"17","author":"Pearson","year":"1976","journal-title":"J. Small Anim. Pract."},{"key":"ref_16","unstructured":"Daleck, C.R., De Nardi, A.B., and Rodaski, S. (2008). Hemangiosarcoma. Oncologia em C\u00e3es e Gatos, Marca."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1016\/0021-9975(78)90012-9","article-title":"Hemangiosarcoma in dogs","volume":"88","author":"Oksanen","year":"1978","journal-title":"J. Comp. Pathol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1111\/j.1939-1676.1991.tb00943.x","article-title":"Efficacy and toxicity of VAC chemotherapy (vincristine, doxorubicin, and cyclophosphamide) in dogs with hemangiosarcoma","volume":"5","author":"Hammer","year":"1991","journal-title":"J. Vet. Inter. Med."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1111\/j.1939-1676.2000.tb02262.x","article-title":"Treatment of canine hemangiosarcoma: 2000 and beyond","volume":"14","author":"Clifford","year":"2000","journal-title":"J. Vet. Inter. Med."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1111\/j.1939-1676.2000.tb02247.x","article-title":"Canine hemangiosracoma treated with standard chemotherapy and minocycline","volume":"14","author":"Sorenmo","year":"2000","journal-title":"J. Vet. Intern. Med."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1016\/S0021-9975(05)80013-1","article-title":"Immunohistochemical detection of CD31 antigen in normal and neoplastic canine endothelial cells","volume":"112","author":"Ferrer","year":"1995","journal-title":"J. Comp. Pathol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1232","DOI":"10.1111\/vco.12259","article-title":"Use of routine histopathology and factor VIII-related antigen\/von Willebrand factor immunohistochemistry to differentiate primary hemangiosarcoma of bone from telangectasic osteosarcoma in 54 dogs","volume":"15","author":"Giuffrida","year":"2017","journal-title":"Vet Comp. Oncol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1158\/0008-5472.CAN-06-3567","article-title":"Vascular endothelial growth factor receptor 3 (VEGFR-3) is involved in tumor angiogenesis and growth","volume":"67","author":"Laakkonen","year":"2007","journal-title":"Cancer Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.jcpa.2018.10.181","article-title":"Canine cutaneous haemangiosarcoma: Biomarkers and survival","volume":"166","author":"Sehaber","year":"2019","journal-title":"J. Comp. Pathol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1016\/j.ccr.2008.04.022","article-title":"VEGFR-3 expression is restricted to blood and lymphatic vessels in solid tumors","volume":"13","author":"Petrova","year":"2008","journal-title":"Cancer Cell"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1002\/vms3.66","article-title":"Pilot assessment of vascular endothelial growth factor receptors and trafficking pathway in recurrent and metastatic canine subcutaneous mast cell tumours","volume":"3","year":"2017","journal-title":"Vet. Med. Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"978","DOI":"10.1038\/modpathol.3880178","article-title":"Splenic angiosarcoma: A clinicopathologic and immunophenotypic study of 28 cases","volume":"13","author":"Neuhauser","year":"2000","journal-title":"Modern Pathol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1111\/j.1939-1676.2006.tb02912.x","article-title":"Vascular endothelial growth factor expression in canine intracranial meningiomas and association with patient survival","volume":"20","author":"Platt","year":"2006","journal-title":"J. Vet. Inter. Med."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"893","DOI":"10.1083\/jcb.153.5.893","article-title":"Membrane-type 1 matrix metalloproteinase cleaves CD44 and promotes cell migration","volume":"153","author":"Kajita","year":"2001","journal-title":"J. Cell Biol."}],"container-title":["Veterinary Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-7381\/7\/3\/102\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T09:53:59Z","timestamp":1760176439000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-7381\/7\/3\/102"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,8,3]]},"references-count":29,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2020,9]]}},"alternative-id":["vetsci7030102"],"URL":"https:\/\/doi.org\/10.3390\/vetsci7030102","relation":{},"ISSN":["2306-7381"],"issn-type":[{"value":"2306-7381","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,8,3]]}}}