{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T17:18:28Z","timestamp":1782407908756,"version":"3.54.5"},"reference-count":41,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2020,9,13]],"date-time":"2020-09-13T00:00:00Z","timestamp":1599955200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"regional association for animal identification and health (Wallonie\/ARSIA)","award":["1"],"award-info":[{"award-number":["1"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>The aim of this study was to identify the pathogens potentially involved in parietal fibrinous peritonitis (PFP). PFP is a complication of laparotomy in cattle, characterized by an accumulation of exudate inside a fibrinous capsule. We have studied 72 cases of PFP in Belgian blue cows, confirmed by a standard diagnostic protocol. Blood was collected to evaluate the presence of antibodies for Mycoplasma bovis(M. bovis), Coxiella burnetii(C. burnetii) and Bovine Herpesvirus 4(BoHV4) by enzyme-linked immunosorbent assays. Peritoneal exudate was obtained from the PFP cavity to perform bacteriological culture, and to identify the DNA of M. bovis, C. burnetii and BoHV4 using real time polymerase chain reaction (qPCR). Bacteriological culture was positive in most peritoneal samples (59\/72); Trueperella pyogenes (T. pyogenes) (51\/72) and Escherichia coli (E. coli) (20\/72) were the most frequently identified. For BoHV4, the majority of cows showed positive serology and qPCR (56\/72 and 49\/72, respectively). Contrariwise, M. bovis (17\/72 and 6\/72, respectively) and C. burnetii (15\/72 and 6\/72, respectively) were less frequently detected (p &lt; 0.0001). Our study proves that PFP can no longer be qualified as a sterile inflammation. Moreover, we herein describe the first identification of BoHV4 and C. burnetii in cows affected by PFP.<\/jats:p>","DOI":"10.3390\/vetsci7030134","type":"journal-article","created":{"date-parts":[[2020,9,13]],"date-time":"2020-09-13T21:11:32Z","timestamp":1600031492000},"page":"134","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Infectious Agents Identified by Real-Time PCR, Serology and Bacteriology in Blood and Peritoneal Exudate Samples of Cows Affected by Parietal Fibrinous Peritonitis after Caesarean Section"],"prefix":"10.3390","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3311-8219","authenticated-orcid":false,"given":"Salem","family":"Djebala","sequence":"first","affiliation":[{"name":"Clinical Department of Ruminants, University of Li\u00e8ge, Quartier Vall\u00e9e 2, Avenue de Cureghem 7A-7D, 4000 Li\u00e8ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julien","family":"Evrard","sequence":"additional","affiliation":[{"name":"Gestion et Pr\u00e9vention de Sant\u00e9, Regional Association of Health and Animal Identification, All\u00e9e des Artisans 2, 5590 Ciney, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabien","family":"Gregoire","sequence":"additional","affiliation":[{"name":"Gestion et Pr\u00e9vention de Sant\u00e9, Regional Association of Health and Animal Identification, All\u00e9e des Artisans 2, 5590 Ciney, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5161-7364","authenticated-orcid":false,"given":"Damien","family":"Thiry","sequence":"additional","affiliation":[{"name":"Bacteriology, Department of Infectious and Parasitic Diseases, University of Li\u00e8ge, Quartier Vall\u00e9e 2, Avenue Cureghem 6, B-4000 Li\u00e8ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2227-4913","authenticated-orcid":false,"given":"Calixte","family":"Bayrou","sequence":"additional","affiliation":[{"name":"Clinical Department of Ruminants, University of Li\u00e8ge, Quartier Vall\u00e9e 2, Avenue de Cureghem 7A-7D, 4000 Li\u00e8ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4438-8759","authenticated-orcid":false,"given":"Nassim","family":"Moula","sequence":"additional","affiliation":[{"name":"Department of Animal Production, University of Liege, Quartier Vall\u00e9e 2, Avenue de Cureghem 6, 4000 Li\u00e8ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arnaud","family":"Sartelet","sequence":"additional","affiliation":[{"name":"Clinical Department of Ruminants, University of Li\u00e8ge, Quartier Vall\u00e9e 2, Avenue de Cureghem 7A-7D, 4000 Li\u00e8ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Bossaert","sequence":"additional","affiliation":[{"name":"Clinical Department of Ruminants, University of Li\u00e8ge, Quartier Vall\u00e9e 2, Avenue de Cureghem 7A-7D, 4000 Li\u00e8ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,9,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1136\/vr.142.13.338","article-title":"Ultrasonographic findings in three cows with peritonitis in the left flank region","volume":"142","author":"Braun","year":"1998","journal-title":"Vet. 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