{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T11:40:16Z","timestamp":1790509216298,"version":"4.1.0"},"reference-count":36,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2021,8,24]],"date-time":"2021-08-24T00:00:00Z","timestamp":1629763200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100018648","name":"Phileo by Lesaffre","doi-asserted-by":"publisher","award":["00002363"],"award-info":[{"award-number":["00002363"]}],"id":[{"id":"10.13039\/501100018648","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Institut national de recherche pour l\u2019agriculture, l\u2019alimentation et l\u2019environnement","award":["00002363"],"award-info":[{"award-number":["00002363"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>Enteric infectious diseases are not all well controlled, which leads to animal suffering and sometimes death in the most severe cases, in addition to economic losses for farmers. Typical symptoms of enteric infections include watery diarrhea, stomach cramps or pain, dehydration, nausea, vomiting, fever and weight loss. Evaluation of new control methods against enteric infections requires the use of many animals. We aimed to develop a new method for an initial in vivo screen of promising compounds against neonatal diseases such as cryptosporidiosis while limiting experimental animal use. We therefore adapted an in vivo method of multiple consecutive but independent intestinal loops to newborn lambs delivered by cesarean section, in which endotoxin responsiveness is retained. This new method allowed for the screening of natural yeast fractions for their ability to stimulate immune responses and to limit early Cryptosporidium parvum development. This model may also be used to investigate host\u2013pathogen interactions and immune responses in a neonatal controlled environment.<\/jats:p>","DOI":"10.3390\/vetsci8090170","type":"journal-article","created":{"date-parts":[[2021,8,24]],"date-time":"2021-08-24T22:09:39Z","timestamp":1629842979000},"page":"170","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Establishment of a Newborn Lamb Gut-Loop Model to Evaluate New Methods of Enteric Disease Control and Reduce Experimental Animal Use"],"prefix":"10.3390","volume":"8","author":[{"given":"Ambre","family":"Baillou","sequence":"first","affiliation":[{"name":"UMR1282 Infectiologie et Sant\u00e9 Publique, INRAE Centre Val de Loire, Universit\u00e9 Fran\u00e7ois Rabelais de Tours, 37380 Nouzilly, France"},{"name":"Phileo by Lesaffre, 137 rue Gabriel P\u00e9ri, 59700 Marcq-en-Bar\u0153ul, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalie","family":"Kasal-Hoc","sequence":"additional","affiliation":[{"name":"UE1277 Plateforme d\u2019Infectiologie Exp\u00e9rimentale (PFIE), INRAE Centre Val de Loire, 37380 Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5912-4652","authenticated-orcid":false,"given":"C\u00e9line","family":"Barc","sequence":"additional","affiliation":[{"name":"UE1277 Plateforme d\u2019Infectiologie Exp\u00e9rimentale (PFIE), INRAE Centre Val de Loire, 37380 Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0908-9833","authenticated-orcid":false,"given":"Juliette","family":"Cogni\u00e9","sequence":"additional","affiliation":[{"name":"UMR85 Physiologie de la reproduction et des Comportements, INRAE Centre Val de Loire, CNRS, IFCE, Universit\u00e9 Fran\u00e7ois Rabelais de Tours, 37380 Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Pinard","sequence":"additional","affiliation":[{"name":"UE1277 Plateforme d\u2019Infectiologie Exp\u00e9rimentale (PFIE), INRAE Centre Val de Loire, 37380 Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00e9r\u00e9my","family":"Pezant","sequence":"additional","affiliation":[{"name":"UE1277 Plateforme d\u2019Infectiologie Exp\u00e9rimentale (PFIE), INRAE Centre Val de Loire, 37380 Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie","family":"Schulthess","sequence":"additional","affiliation":[{"name":"Phileo by Lesaffre, 137 rue Gabriel P\u00e9ri, 59700 Marcq-en-Bar\u0153ul, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pauline","family":"Peltier-Pain","sequence":"additional","affiliation":[{"name":"Phileo by Lesaffre, 137 rue Gabriel P\u00e9ri, 59700 Marcq-en-Bar\u0153ul, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7865-4587","authenticated-orcid":false,"given":"Sonia","family":"Lacroix-Lamand\u00e9","sequence":"additional","affiliation":[{"name":"UMR1282 Infectiologie et Sant\u00e9 Publique, INRAE Centre Val de Loire, Universit\u00e9 Fran\u00e7ois Rabelais de Tours, 37380 Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabrice","family":"Laurent","sequence":"additional","affiliation":[{"name":"UMR1282 Infectiologie et Sant\u00e9 Publique, INRAE Centre Val de Loire, Universit\u00e9 Fran\u00e7ois Rabelais de Tours, 37380 Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,8,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1016\/j.coph.2009.08.004","article-title":"Novel trends in high-throughput screening","volume":"9","author":"Mayr","year":"2009","journal-title":"Curr. 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