{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T02:39:56Z","timestamp":1760236796276,"version":"build-2065373602"},"reference-count":34,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2021,12,24]],"date-time":"2021-12-24T00:00:00Z","timestamp":1640304000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>An adult female osprey (Pandion haliaetus) was found weak and unable to fly in Auburn, Alabama in August 2019. The bird was captured and submitted to the Southeastern Raptor Center of the Auburn University College of Veterinary Medicine for evaluation. On presentation, the bird was thin with a body condition score of approximately 1.5 out of 5. The bird died during the examination and was submitted for necropsy. At the necropsy, there was a severe loss of muscle mass over the body, and the keel was prominent. The liver and spleen were moderately enlarged with pale tan to red foci randomly scattered throughout the parenchyma. A histopathologic observation revealed multifocal to coalescing areas of necrosis and hemorrhage with intralesional protozoans in the liver, spleen, lungs, kidney, sciatic nerve, esophagus, cerebrum, heart, and proventriculus. Immunohistochemistry using anti-Toxoplasma gondii-specific antibodies showed a strong positive labeling of the parasite. Semi-nested PCR, specific for the B1 gene of T. gondii, successfully identified T. gondii. This is the first confirmed case of T. gondii infection in an osprey.<\/jats:p>","DOI":"10.3390\/vetsci9010005","type":"journal-article","created":{"date-parts":[[2021,12,27]],"date-time":"2021-12-27T01:03:37Z","timestamp":1640567017000},"page":"5","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Disseminated Toxoplasma gondii Infection in an Adult Osprey (Pandion haliaetus)"],"prefix":"10.3390","volume":"9","author":[{"given":"Xiaobo","family":"Wang","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, Yangzhou University; Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou University, Yangzhou 225012, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6136-1991","authenticated-orcid":false,"given":"Charles T.","family":"Talbot","sequence":"additional","affiliation":[{"name":"Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, AL 36849, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6242-5077","authenticated-orcid":false,"given":"Ji-Hang","family":"Yin","sequence":"additional","affiliation":[{"name":"Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, AL 36849, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anwar A.","family":"Kalalah","sequence":"additional","affiliation":[{"name":"Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, AL 36849, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7874-8340","authenticated-orcid":false,"given":"Chengming","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, AL 36849, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph C.","family":"Newton","sequence":"additional","affiliation":[{"name":"Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, AL 36849, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,12,24]]},"reference":[{"key":"ref_1","unstructured":"Dubey, J.P., and Odening, K. (2001). Toxoplasmosis and Related Infections, Iowa State University Press."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"937","DOI":"10.1645\/GE-2501.1","article-title":"Serological and parasitological prevalence of Toxoplasma gondii in wild birds from Colorado","volume":"96","author":"Dubey","year":"2010","journal-title":"J. Parasitol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"e00049","DOI":"10.1016\/j.fawpar.2019.e00049","article-title":"Environmental transmission of Toxoplasma gondii: Oocysts in water, soil and food","volume":"15","author":"Shapiro","year":"2019","journal-title":"Food Waterborne Parasitol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1217","DOI":"10.1016\/S0020-7519(00)00124-7","article-title":"Toxoplasma gondii: From animals to humans","volume":"30","author":"Tenter","year":"2000","journal-title":"Int. J. Parasitol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1146\/annurev-micro-091014-104100","article-title":"Lytic Cycle of Toxoplasma gondii: 15 Years Later","volume":"69","author":"Blader","year":"2015","journal-title":"Annu. Rev. Microbiol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1965","DOI":"10.1016\/S0140-6736(04)16412-X","article-title":"Toxoplasmosis","volume":"363","author":"Montoya","year":"2004","journal-title":"Lancet"},{"key":"ref_7","first-page":"389","article-title":"Toxoplasma gondii Infection in Immunocompromised Patients: A Systematic Review and Meta-Analysis","volume":"8","author":"Wang","year":"2017","journal-title":"Front. Microbiol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1007\/s40588-019-0114-5","article-title":"Toxoplasma: Immunity and Pathogenesis","volume":"6","author":"Khan","year":"2019","journal-title":"Curr. Clin. Microbiol. Rep."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.pt.2016.10.007","article-title":"Lifelong Persistence of Toxoplasma Cysts: A Questionable Dogma?","volume":"33","author":"Rougier","year":"2017","journal-title":"Trends Parasitol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"4121","DOI":"10.1128\/JCM.38.11.4121-4125.2000","article-title":"Real-time PCR for quantitative detection of Toxoplasma gondii","volume":"38","author":"Lin","year":"2000","journal-title":"J. Clin. Microbiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/S0020-7519(99)00170-8","article-title":"Identification of a 200- to 300-fold repetitive 529 bp DNA fragment in Toxoplasma gondii, and its use for diagnostic and quantitative PCR","volume":"30","author":"Homan","year":"2000","journal-title":"Int. J. Parasitol."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Wright, D.K., and Manos, M.M. (1990). Sample Preparation from Paraffin-Embedded Tissues, Academic.","DOI":"10.1016\/B978-0-12-372180-8.50023-8"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"870","DOI":"10.2307\/3283724","article-title":"Prevalence of encysted Toxoplasma gondii in raptors from Alabama","volume":"79","author":"Lindsay","year":"1993","journal-title":"J. Parasitol."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Bierregaard, R. (2020). Osprey (Pandion haliaetus) Birds of the World. Cornell Laboratory of Ornithology, Ithaca.","DOI":"10.2173\/bow.osprey.02"},{"key":"ref_15","unstructured":"Bierregaard, R. (2021, August 12). The Cornell Lab of Ornithology. Birds of the World. Available online: https:\/\/birdsoftheworld.org\/bow\/species\/osprey\/cur\/introduction."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"907","DOI":"10.1645\/GE-270R","article-title":"Fatal toxoplasmosis in a bald eagle (Haliaeetus leucocephalus)","volume":"90","author":"Szabo","year":"2004","journal-title":"J. Parasitol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"653","DOI":"10.7589\/2015-10-269","article-title":"Antibody Prevalence and Isolation of Viable Toxoplasma gondii from Raptors in the Southeastern USA","volume":"52","author":"Love","year":"2016","journal-title":"J. Wildl. Dis."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"738","DOI":"10.2307\/1592169","article-title":"Severe hepatitis resulting from toxoplasmosis in a barred owl (Strix varia) from Qu\u00e9bec, Canada","volume":"41","author":"Mikaelian","year":"1997","journal-title":"Avian Dis."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Cabez\u00f3n, O., Garc\u00eda-Bocanegra, I., Molina-L\u00f3pez, R., Marco, I., Blanco, J.M., H\u00f6fle, U., Margalida, A., Bach-Raich, E., Darwich, L., and Echeverr\u00eda, I. (2011). Seropositivity and risk factors associated with Toxoplasma gondii infection in wild birds from Spain. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0029549"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"322","DOI":"10.2307\/3283107","article-title":"Toxoplasma gondii infections in red-tailed hawks inoculated orally with tissue cysts","volume":"77","author":"Lindsay","year":"1991","journal-title":"J. Parasitol."},{"key":"ref_21","first-page":"98","article-title":"Induced Toxoplasmosis in Owls","volume":"23","author":"Dubey","year":"1992","journal-title":"J. Zoo Wildl. Med."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1016\/S0168-6445(03)00071-8","article-title":"How to detect Toxoplasma gondii oocysts in environmental samples?","volume":"27","year":"2003","journal-title":"FEMS Microbiol. Rev."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"862","DOI":"10.2307\/3284606","article-title":"Toxoplasma gondii oocyst survival under defined temperatures","volume":"84","author":"Dubey","year":"1998","journal-title":"J. Parasitol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1019","DOI":"10.1645\/GE-1919.1","article-title":"Long-term survival of Toxoplasma gondii sporulated oocysts in seawater","volume":"95","author":"Lindsay","year":"2009","journal-title":"J. Parasitol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1645\/GE-1342.1","article-title":"Risk factors for Toxoplasma gondii infection in wild rodents from central coastal California and a review of T. gondii prevalence in rodents","volume":"94","author":"Dabritz","year":"2008","journal-title":"J. Parasitol."},{"key":"ref_26","unstructured":"Miller, M. (2018). Protozoan Parasites of Marine Mammals, CRC Press."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"826","DOI":"10.1111\/zph.12630","article-title":"Toxoplasma gondii in edible fishes captured in the Mediterranean basin","volume":"66","author":"Marino","year":"2019","journal-title":"Zoonoses Public Health"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1016\/j.vetpar.2010.01.002","article-title":"Uptake and transmission of Toxoplasma gondii oocysts by migratory, filter-feeding fish","volume":"169","author":"Massie","year":"2010","journal-title":"Vet. Parasitol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1087","DOI":"10.1016\/S0020-7519(03)00181-4","article-title":"Molecular and bioassay-based detection of Toxoplasma gondii oocyst uptake by mussels (Mytilus galloprovincialis)","volume":"33","author":"Arkush","year":"2003","journal-title":"Int J. Parasitol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1054","DOI":"10.1645\/GE-296R","article-title":"Survival of Toxoplasma gondii oocysts in Eastern oysters (Crassostrea virginica)","volume":"90","author":"Lindsay","year":"2004","journal-title":"J. Parasitol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"4527","DOI":"10.1111\/1462-2920.12927","article-title":"Concentration and retention of Toxoplasma gondii oocysts by marine snails demonstrate a novel mechanism for transmission of terrestrial zoonotic pathogens in coastal ecosystems","volume":"17","author":"Krusor","year":"2015","journal-title":"Environ. Microbiol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1703","DOI":"10.1017\/S0031182016001359","article-title":"Concentration and retention of Toxoplasma gondii surrogates from seawater by red abalone (Haliotis rufescens)","volume":"143","author":"Schott","year":"2016","journal-title":"Parasitology"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1496","DOI":"10.1645\/GE-3503RN.1","article-title":"Toxoplasma gondii does not persist in goldfish (Carassius auratus)","volume":"91","author":"Omata","year":"2005","journal-title":"J. Parasitol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1111\/jfd.12393","article-title":"The zebrafish, Danio rerio, as a model for Toxoplasma gondii: An initial description of infection in fish","volume":"38","author":"Sanders","year":"2015","journal-title":"J. Fish. 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