{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T19:27:07Z","timestamp":1785785227038,"version":"3.56.0"},"reference-count":66,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2022,1,22]],"date-time":"2022-01-22T00:00:00Z","timestamp":1642809600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>In Algeria, data on the epidemiology of coxiellosis in cattle are still lacking. In this study, bulk tank milk (BTM) samples from 200 randomly selected dairy cattle herds from Setif province of Algeria were analyzed by an indirect enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR). Results highlighted that 37% (95% CI: 30.31\u201343.69%) and 9% (95% CI: 5.03\u201312.96%) of BTM samples contained Coxiella burnetii antibodies and DNA, respectively. Based on Cohen\u2019s kappa coefficient, a very low agreement between the ELISA and PCR results was found (k = 0.0849) (95% CI: 0.00\u20130.189). For a second experiment, 186 whole blood samples of cows from farms with reproduction disorders were analyzed by molecular tools to detect C. burnetii. This study revealed an overall prevalence of 6.98% (95% CI: 3.32\u201310.65%). All positive samples determined by conventional PCR were analyzed by real-time quantitative PCR (qPCR). Eleven samples with cycle threshold (Ct) values lower than 35 were selected for genotyping by the multispacer sequence typing (MST) method. The MST12 genotype in BTM samples, the MST32 genotype and a new MST genotype (partial profile) in whole blood samples were identified. Obtained results have allowed us to better understand the epidemiology of bovine coxiellosis in the region of Setif.<\/jats:p>","DOI":"10.3390\/vetsci9020040","type":"journal-article","created":{"date-parts":[[2022,1,23]],"date-time":"2022-01-23T20:36:27Z","timestamp":1642970187000},"page":"40","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Serological, Molecular Prevalence and Genotyping of Coxiella burnetii in Dairy Cattle Herds in Northeastern Algeria"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1257-1076","authenticated-orcid":false,"given":"Salah Eddine","family":"Menadi","sequence":"first","affiliation":[{"name":"Research Laboratory Management of Local Animal Resources, High National Veterinary School \u201cRABIE BOUCHAMA\u201d, Street Issad Abbes, Oued Smar, Algiers 16270, Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3907-0463","authenticated-orcid":false,"given":"Valentina","family":"Chisu","sequence":"additional","affiliation":[{"name":"Zoonotic Pathology and OIE Reference Laboratory for Echinococcosis, National Reference Center for Echinococcosis (CeNRE), Istituto Zooprofilattico Sperimentale della Sardegna, 07100 Sassari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9699-3745","authenticated-orcid":false,"given":"Cinzia","family":"Santucciu","sequence":"additional","affiliation":[{"name":"Zoonotic Pathology and OIE Reference Laboratory for Echinococcosis, National Reference Center for Echinococcosis (CeNRE), Istituto Zooprofilattico Sperimentale della Sardegna, 07100 Sassari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4795-8323","authenticated-orcid":false,"given":"Marco","family":"Di Domenico","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale dell\u2019Abruzzo e del Molise G. Caporale, 64100 Teramo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2335-3154","authenticated-orcid":false,"given":"Valentina","family":"Curini","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale dell\u2019Abruzzo e del Molise G. Caporale, 64100 Teramo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanna","family":"Masala","sequence":"additional","affiliation":[{"name":"Zoonotic Pathology and OIE Reference Laboratory for Echinococcosis, National Reference Center for Echinococcosis (CeNRE), Istituto Zooprofilattico Sperimentale della Sardegna, 07100 Sassari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,1,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1128\/CMR.00045-16","article-title":"From Q fever to Coxiella burnetii infection: A paradigm change","volume":"30","author":"Eldin","year":"2017","journal-title":"Clin. Microbiol. 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