{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T13:18:44Z","timestamp":1771334324999,"version":"3.50.1"},"reference-count":65,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2022,5,19]],"date-time":"2022-05-19T00:00:00Z","timestamp":1652918400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Saudi Ministry of Environment, Water, and Agriculture by General Administration of Animal Productions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>A lipopolysaccharide (LPS) is a large molecule and an outer membrane glycolipid found in Gram-negative bacteria, including Escherichia coli (E. coli). These molecules (LPS) target acute inflammatory responses and significant physiological changes. Importantly, E. coli is considered one of the most important bacterial causes of avian colibacillosis that affect domestic turkey industry. However, little information is available about the potential influence of LPS on the biochemical parameters and histopathological changes in turkey poults. Therefore, this study aimed to evaluate the influence of bacterial lipopolysaccharide (LPS) molecules on serum biomarkers and histopathological changes in turkey poults. The birds were randomly divided into five groups, as follows: group I did not receive any inoculation; group II was inoculated with sterile saline; and groups III, IV, and V were inoculated intraperitoneally with LPS at 0.01, 0.1, and 1 mg\/kg of body weight (BW), respectively. The biochemical parameters and the histopathology of different organs were examined in all birds one day post-inoculation. Our results revealed hypolipidemia, hypoglycemia, a significant decrease in uric acid, and a significant increase in serum activities of aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), and creatine kinase (CK), as well as cardiac troponin T concentrations in treated groups. Moreover, there was a significant increase in \u03b11-, \u03b2-, and \u03b3-globulin concentrations and a decrease in albumin and \u03b12-globulin concentrations in group V. However, a significant increase in \u03b12- and \u03b3-globulin levels and a decrease in albumin levels were detected in groups III and IV. In addition, significant decreases in the albumin\/globulin ratio were recorded in all LPS-treated groups. Hepatocellular and cardiac muscle necrosis, slight renal changes, and massive pulmonary inflammatory reactions were recorded. This study provides valuable information about serum biomarkers, protein fractions, and histopathological changes in turkey poults treated with LPS for further investigations of pathophysiological mechanisms in avian medicine along with biomedical research.<\/jats:p>","DOI":"10.3390\/vetsci9050240","type":"journal-article","created":{"date-parts":[[2022,5,20]],"date-time":"2022-05-20T00:18:11Z","timestamp":1653005891000},"page":"240","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["The Effects of Bacterial Lipopolysaccharide (LPS) on Turkey Poults: Assessment of Biochemical Parameters and Histopathological Changes"],"prefix":"10.3390","volume":"9","author":[{"given":"Mohamed F.","family":"Abou Elazab","sequence":"first","affiliation":[{"name":"Clinical Pathology Department, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafr El-Sheikh 33516, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nasr E.","family":"Nasr","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Clinical Biochemistry, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafr El-Sheikh 33516, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamed S.","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Pathology Department, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafr El-Sheikh 33516, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barakat M.","family":"Alrashdi","sequence":"additional","affiliation":[{"name":"Biology Department, College of Science, Jouf University, Sakaka 72388, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3560-8166","authenticated-orcid":false,"given":"Naief","family":"Dahran","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Faculty of Medicine, University of Jeddah, Jeddah 21959, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3536-9276","authenticated-orcid":false,"given":"Mohamed A.","family":"Alblihed","sequence":"additional","affiliation":[{"name":"Department of Microbiology, College of Medicine, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4484-3678","authenticated-orcid":false,"given":"Ehab Kotb","family":"Elmahallawy","sequence":"additional","affiliation":[{"name":"Department of Zoonoses, Faculty of Veterinary Medicine, Sohag University, Sohag 82524, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,5,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"fnv118","DOI":"10.1093\/femsle\/fnv118","article-title":"Avian colibacillosis: Still many black holes","volume":"362","author":"Guabiraba","year":"2015","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_2","unstructured":"Hafez, H. (2005, January 22\u201326). Current knowledge and prospective risk analysis related to ongoing turkey diseases. 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