{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:13:38Z","timestamp":1792790018979,"version":"4.5.0"},"reference-count":53,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2022,6,16]],"date-time":"2022-06-16T00:00:00Z","timestamp":1655337600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"USAMVB Timisoara","award":["6PFE"],"award-info":[{"award-number":["6PFE"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>Staphylococcal infection treatment in dogs is frequently associated with adverse side effects, high costs, prolonged treatment, and resistant strain selection. Staphylococcus aureus is the most frequently isolated staphylococci in cases of canine superficial pyoderma. The number of Staphylococcus strains to exhibit primary resistance to various drugs in vitro is increasing. Propolis has a diverse chemical composition and well-known therapeutic properties against bacterial infections. The current investigation evaluated in vitro the antimicrobial activity of the commercial allopathic antimicrobials, Romanian propolis ethanolic extracts, against clinical Staphylococcus aureus strains isolated from superficial dermatitis clinical samples in dogs and two reference strains: Staphylococcus aureus ATCC 25923 and Staphylococcus aureus ATCC 43300, as the MSSA and MRSA positive controls, respectively, in western Romania. We used the microdilution broth technique to evaluate the susceptibility profile of the bacteria. The minimum inhibitory concentration (MIC) of the Romanian propolis ethanolic extract ranged from 6 to 10 \u03bcg\/mL for all isolates, determined by the broth microdilution method. The MICs of ethanolic Romanian propolis extracts had a pronounced antibacterial activity. These results indicate that propolis can potentially be used and recommended for in vivo experiments as a promising therapeutic agent against Staphylococcus aureus infections in superficial dermatitis of dogs.<\/jats:p>","DOI":"10.3390\/vetsci9060299","type":"journal-article","created":{"date-parts":[[2022,6,19]],"date-time":"2022-06-19T22:54:52Z","timestamp":1655679292000},"page":"299","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Antibacterial Activity of Romanian Propolis against Staphylococcus aureus Isolated from Dogs with Superficial Pyoderma: In Vitro Test"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5859-5057","authenticated-orcid":false,"given":"J\u00e1nos","family":"D\u00e9gi","sequence":"first","affiliation":[{"name":"Faculty of Veterinary Medicine, Banat\u2019s University of Agricultural Sciences and Veterinary Medicine Timi\u0219oara, Calea Aradului 119, 300645 Timi\u0219oara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7118-6488","authenticated-orcid":false,"given":"Viorel","family":"Herman","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Banat\u2019s University of Agricultural Sciences and Veterinary Medicine Timi\u0219oara, Calea Aradului 119, 300645 Timi\u0219oara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7314-4641","authenticated-orcid":false,"given":"Violeta","family":"Igna","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Banat\u2019s University of Agricultural Sciences and Veterinary Medicine Timi\u0219oara, Calea Aradului 119, 300645 Timi\u0219oara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana Maria","family":"D\u00e9gi","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Banat\u2019s University of Agricultural Sciences and Veterinary Medicine Timi\u0219oara, Calea Aradului 119, 300645 Timi\u0219oara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anca","family":"Hulea","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Banat\u2019s University of Agricultural Sciences and Veterinary Medicine Timi\u0219oara, Calea Aradului 119, 300645 Timi\u0219oara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2907-4233","authenticated-orcid":false,"given":"Florin","family":"Muselin","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Banat\u2019s University of Agricultural Sciences and Veterinary Medicine Timi\u0219oara, Calea Aradului 119, 300645 Timi\u0219oara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5420-1516","authenticated-orcid":false,"given":"Romeo Teodor","family":"Cristina","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Banat\u2019s University of Agricultural Sciences and Veterinary Medicine Timi\u0219oara, Calea Aradului 119, 300645 Timi\u0219oara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Dezmirean, D.S., Pa\u015fca, C., Moise, A.R., and Bobi\u015f, O. (2021). Plant Sources Responsible for the Chemical Composition and Main Bioactive Properties of Poplar-Type Propolis. Plants, 10.","DOI":"10.3390\/plants10010022"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Zulhendri, F., Chandrasekaran, K., Kowacz, M., Ravalia, M., Kripal, K., Fearnley, J., and Perera, C.O. (2021). Antiviral, Antibacterial, Antifungal, and Antiparasitic Properties of Propolis: A Review. Foods, 10.","DOI":"10.3390\/foods10061360"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Przyby\u0142ek, I., and Karpi\u0144ski, T.M. (2019). Antibacterial Properties of Propolis. Molecules, 24.","DOI":"10.3390\/molecules24112047"},{"key":"ref_4","first-page":"423","article-title":"Flavonoid analysis and antimicrobial activity of commercially available propolis products","volume":"55","author":"Kosalec","year":"2005","journal-title":"Acta Pharm"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"376","DOI":"10.17221\/103\/2017-CJFS","article-title":"Components responsible for antimicrobial activity of propolis from continental and Mediterranean regions in Croatian","volume":"35","author":"Tlak","year":"2017","journal-title":"Czech J. Food Sci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/j.micres.2018.05.003","article-title":"Brazilian red propolis: Chemical composition and antibacterial activity determined using bioguided fractionation","volume":"214","author":"Rufatto","year":"2018","journal-title":"Microbiol. Res."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Palma, E., Tilocca, B., and Roncada, P. (2020). Antimicrobial Resistance in Veterinary Medicine: An Overview. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21061914"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"J\u00e1nos, D., Viorel, H., Ionica, I., Corina, P., Tiana, F., and Roxana, D. (2021). Carriage of Multidrug Resistance Staphylococci in Shelter Dogs in Timisoara, Romania. Antibiotics, 10.","DOI":"10.3390\/antibiotics10070801"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1136\/vr.101426","article-title":"Frequency of isolation and antibiotic resistance of stapylococcal flora from external otitis of dogs","volume":"173","author":"Imre","year":"2013","journal-title":"Vet. Rec."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Lindsay, J.A. (2019). Staphylococci: Evolving Genomes. Microbiol. Spectr., 7.","DOI":"10.1128\/microbiolspec.GPP3-0071-2019"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"304-e69","DOI":"10.1111\/vde.12444","article-title":"Recommendations for approaches to meticillin-resistant staphylococcal infections of small animals: Diagnosis, therapeutic considerations and preventative measures. Clinical Consensus Guidelines of the World Association for Veterinary Dermatology","volume":"28","author":"Morris","year":"2017","journal-title":"Vet. Dermatol."},{"key":"ref_12","first-page":"5","article-title":"Main laboratory methods used for the isolation and identification of Staphylococcus spp.","volume":"50","author":"Moraes","year":"2021","journal-title":"Rev. Colomb. Cienc. Qu\u00edm. Farm."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"376","DOI":"10.3389\/fvets.2020.00376","article-title":"Molecular Detection and Characterization of the mecA and nuc Genes from Staphylococcus Species (S. aureus, S. pseudintermedius, and S. schleiferi) Isolated from Dogs Suffering Superficial Pyoderma and Their Antimicrobial Resistance Profiles","volume":"7","author":"Carvajal","year":"2020","journal-title":"Front. Vet. Sci."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Al-Ani, I., Zimmermann, S., Reichling, J., and Wink, M. (2018). Antimicrobial Activities of European Propolis Collected from Various Geographic Origins Alone and in Combination with Antibiotics. Medicines, 5.","DOI":"10.3390\/medicines5010002"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1002\/pca.2544","article-title":"Ultrahigh-performance liquid chromatography and mass spectrometry (UHPLC-LTQ\/Orbitrap\/MS\/MS) study of phenolic profile of Serbian poplar type propolis","volume":"26","year":"2015","journal-title":"Phytochem. Anal."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/00218839.2016.1222661","article-title":"Standard methods for Apis mellifera propolis research","volume":"58","author":"Bankova","year":"2019","journal-title":"J. Apic. Res."},{"key":"ref_17","unstructured":"(2022, April 26). Biosafety Manual of the University Veterinary Clinics of Timisoara. Available online: https:\/\/www.usab-tm.ro\/utilizatori\/medicinaveterinara\/file\/Manual%20Biosecuritate%20CVU%202019.pdf."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"9623","DOI":"10.3390\/molecules18089623","article-title":"Susceptibility of Staphylococcus aureus Clinical Isolates to Propolis Extract Alone or in Combination with Antimicrobial Drugs","volume":"18","author":"Wojtyczka","year":"2013","journal-title":"Molecules"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"4211","DOI":"10.1128\/JCM.40.11.4211-4217.2002","article-title":"Optimal DNA isolation method for detection of bacteria in clinical specimens by broad-range PCR","volume":"40","author":"Jalava","year":"2002","journal-title":"J. Clin. Microbiol."},{"key":"ref_20","unstructured":"(2012). Clinical and Laboratory Standards Institute Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically\u2014Approved Standards\u2014Ninth Edition (Standard No. M07-A9)."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"19610","DOI":"10.3390\/molecules191219610","article-title":"Recent advances in the chemical composition of propolis","volume":"19","author":"Huang","year":"2014","journal-title":"Molecules"},{"key":"ref_22","unstructured":"(2013). Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals\u2014Approved Standard\u20144th ed (Standard No. CLSI M31-A4\/VET01-A4)."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"e00203-19","DOI":"10.1128\/JCM.00203-19","article-title":"Understanding and Addressing CLSI Breakpoint Revisions: A Primer for Clinical Laboratories","volume":"57","author":"Humphries","year":"2019","journal-title":"J. Clin. Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"4665","DOI":"10.1128\/AAC.48.12.4665-4672.2004","article-title":"Impact of high-inoculum Staphylococcus aureus on the activities of nafcillin, vancomycin, linezolid, and daptomycin, alone and in combination with gentamicin, in an in vitro pharmacodynamic model","volume":"48","author":"LaPlante","year":"2004","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1038\/nprot.2007.521","article-title":"Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances","volume":"3","author":"Wiegand","year":"2008","journal-title":"Nat. Protoc."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Baquer, F., Sawan, A.A., Auzou, M., Grillon, A., Jaulhac, B., Join-Lambert, O., and Boyer, P.H. (2021). Broth Microdilution and Gradient Diffusion Strips vs. Reference Agar Dilution Method: First Evaluation for Clostridiales Species Antimicrobial Susceptibility Testing. Antibiotics, 10.","DOI":"10.3390\/antibiotics10080975"},{"key":"ref_27","unstructured":"Mihai, C.M. (2011). Evaluation of Propolis Quality from Transilvania with Regards to Standardization. [Ph.D. Thesis, University of Agronomic Sciences and Veterinary Medicine]."},{"key":"ref_28","first-page":"56","article-title":"Flavonoid contents of propolis from the West Side of Romania and correlation with the antioxidant activity","volume":"53","author":"Coneac","year":"2008","journal-title":"Chem. Bull. Politeh. Univ. Timi\u015foara"},{"key":"ref_29","first-page":"159392","article-title":"Important Developments in Romanian Propolis Research","volume":"2013","author":"Dezmirean","year":"2013","journal-title":"Evid. Based Complement. Alternat. Med."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Bouchelaghem, S., Das, S., Naorem, R.S., Czuni, L., Papp, G., and Kocsis, M. (2022). Evaluation of Total Phenolic and Flavonoid Contents, Antibacterial and Antibiofilm Activities of Hungarian Propolis Ethanolic Extract against Staphylococcus aureus. Molecules, 27.","DOI":"10.3390\/molecules27020574"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1093\/ecam\/nem177","article-title":"Seasonal variation, chemical composition and antioxidant activity of Brazilian propolis samples","volume":"7","author":"Teixeira","year":"2010","journal-title":"Evid. Based Comp. Alter. Med."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1256","DOI":"10.1002\/ptr.2480","article-title":"Comparative study of the antibacterial activity of propolis from different geographical and climatic zones","volume":"22","author":"Seidel","year":"2008","journal-title":"Phytother. Res."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1016\/j.anaerobe.2007.02.001","article-title":"In vitro antimicrobial activity of propolis samples from different geographical origins against certain oral pathogens","volume":"13","author":"Koru","year":"2007","journal-title":"Anaerobe"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1021\/jm0004190","article-title":"Flavonolignan and flavone inhibitors of a Staphylococcus aureus multidrug resistance pump: Structure-activity relationships","volume":"44","author":"Guz","year":"2001","journal-title":"J. Med. Chem."},{"key":"ref_35","first-page":"13","article-title":"Evaluation of propolis for antibacterial activity in vitro","volume":"28","author":"Herman","year":"2018","journal-title":"Rev. Rom. Med. Vet."},{"key":"ref_36","first-page":"180","article-title":"Some correlations between environmental parameters and the foraging behaviour of honeybees (Apismellifera) on oilseed rape (Brassica napusoleifera)","volume":"LXII","author":"Simiz","year":"2019","journal-title":"Sci. Pap. Ser. D Anim. Sci."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1111\/jam.13400","article-title":"Artepillin C and phenolic compounds responsible for antimicrobial and antioxidant activity of green propolis and Baccharis dracunculifolia DC","volume":"122","author":"Veiga","year":"2017","journal-title":"J. Appl. Microbiol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.foodchem.2019.02.078","article-title":"Development of propolis nanoemulsion with antioxidant and antimicrobial activity for use as a potential natural preservative","volume":"287","author":"Seibert","year":"2019","journal-title":"Food Chem."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"887","DOI":"10.1007\/s00216-009-3232-8","article-title":"Phenolic characterization of Northeast Portuguese propolis: Usual and unusual compounds","volume":"396","author":"Estevinho","year":"2010","journal-title":"Anal. Bioanal Chem."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1002\/pca.2412","article-title":"Phenolic profiling of Portuguese propolis by LC-MS spectrometry: Uncommon propolis rich in flavonoid glycosides","volume":"24","author":"Vale","year":"2013","journal-title":"Phytochem. Anal."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"3079","DOI":"10.1016\/j.sjbs.2020.09.016","article-title":"Propolis as a novel antibacterial agent","volume":"27","author":"Almuhayawi","year":"2020","journal-title":"Saudi J. Biol. Sci."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Silva-Carvalho, R., Baltazar, F., and Almeida-Aguiar, C. (2015). Propolis: A Complex Natural Product with a Plethora of Biological Activities That Can Be Explored for Drug Development. Evid. Based Comp. Alter. Med. eCAM, 206439.","DOI":"10.1155\/2015\/206439"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1016\/j.jep.2010.10.032","article-title":"Propolis: Is there a potential for the development of new drugs?","volume":"133","author":"Sforcin","year":"2011","journal-title":"J. Ethnopharmacol."},{"key":"ref_44","first-page":"97","article-title":"Aktywno\u2019s\u2019c antybiotyczna propolisu krajowego i europejskiego. The antibiotic activity of native and european propolis","volume":"2","author":"Kedzia","year":"2013","journal-title":"Postepy. Fitoter."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"C1188","DOI":"10.1111\/1750-3841.12881","article-title":"International regulations of propolis quality: Required assays do not necessarily reflect their polyphenolicrelated in vitro activities","volume":"80","author":"Bridi","year":"2015","journal-title":"J. Food Sci."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1259","DOI":"10.1590\/S1517-83822011000400002","article-title":"Evaluation of the in vitro antimicrobial activity of an ethanol extract of Brazilian classified propolis on strains of Staphylococcus aureus","volume":"42","author":"Pamplona","year":"2011","journal-title":"Braz. J. Microbiol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/00218839.2020.1739410","article-title":"The Coloss Beebok: Global standards in honey bee research","volume":"59","author":"Norman","year":"2020","journal-title":"J. Apic. Res."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"6484","DOI":"10.1021\/jf201280z","article-title":"Cuban and Brazilian red propolis: Botanical origin and comparative analysis by high-performance liquid chromatography-photodiode array detection\/electrospray ionization tandem mass spectrometry","volume":"59","author":"Piccinelli","year":"2011","journal-title":"J. Agric. Food Chem."},{"key":"ref_49","first-page":"267878","article-title":"Comparative chemistry of propolis from eight brazilian localities","volume":"2013","author":"Righi","year":"2013","journal-title":"Evid. Based Comp. Alter. Med. eCAM"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Galeotti, F., Maccari, F., Fachini, A., and Volpi, N. (2018). Chemical Composition and Antioxidant Activity of Propolis Prepared in Different Forms and in Different Solvents Useful for Finished Products. Foods, 7.","DOI":"10.3390\/foods7030041"},{"key":"ref_51","first-page":"308249","article-title":"Propolis: A Wonder Bees Product and Its Pharmacological Potentials","volume":"2013","author":"Wagh","year":"2013","journal-title":"Adv. Pharmacol. Sci."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1695","DOI":"10.1016\/j.sjbs.2018.08.013","article-title":"Composition and functional properties of propolis (bee glue): A review","volume":"26","author":"Anjum","year":"2019","journal-title":"Saudi J. Biol. Sci."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"725","DOI":"10.12691\/jfnr-7-10-6","article-title":"A Great Concern Regarding the Authenticity Identification and Quality Control of Chinese Propolis and Brazilian Green Propolis","volume":"7","author":"Sun","year":"2019","journal-title":"J. Food Nutr. Res."}],"container-title":["Veterinary Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-7381\/9\/6\/299\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T23:32:42Z","timestamp":1760139162000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-7381\/9\/6\/299"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6,16]]},"references-count":53,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2022,6]]}},"alternative-id":["vetsci9060299"],"URL":"https:\/\/doi.org\/10.3390\/vetsci9060299","relation":{},"ISSN":["2306-7381"],"issn-type":[{"value":"2306-7381","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,6,16]]}}}