{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,17]],"date-time":"2025-11-17T15:11:11Z","timestamp":1763392271339,"version":"3.45.0"},"reference-count":34,"publisher":null,"issue":"7","license":[{"start":{"date-parts":[[2022,7,16]],"date-time":"2022-07-16T00:00:00Z","timestamp":1657929600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Texas Tech University Health Sciences Center, El Paso Texas"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>Gastrointestinal (GI) dysmotility in diabetics exhibits fecal incontinence or constipation which affects patients\u2019 quality of life. In this study, we aimed to understand the pattern of GI transit in type 1 diabetic (T1D) mice and whether inhibiting endocannabinoid degradation would exhibit therapeutic effect. Whole gut-transit time and fecal-pellet output were measured at 16 week post-diabetes. T1D mice treated with fatty acid amide hydrolase (FAAH) inhibitor URB597 showed reduced fecal output as well as improved gut transit time. Cannabinoid 1 receptor antagonist, AM251 blocked the effects of URB597, which may demonstrate that FAAH inhibitor is a potential remedial strategy for GI dysmotility.<\/jats:p>","DOI":"10.3390\/vetsci9070364","type":"journal-article","created":{"date-parts":[[2022,7,17]],"date-time":"2022-07-17T23:53:22Z","timestamp":1658102002000},"page":"364","source":"Crossref","is-referenced-by-count":0,"title":["Inhibiting Fatty Acid Amide Hydrolase Ameliorates Enteropathy in Diabetic Mice: A Cannabinoid 1 Receptor Mediated Mechanism"],"prefix":"10.3390","volume":"9","author":[{"given":"Vikram","family":"Thakur","sequence":"first","affiliation":[{"name":"Center of Emphasis in Diabetes and Metabolism, Department of Molecular and Translational Medicine, Texas Tech University Health Sciences Center El Paso, El Paso, TX 79905, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad","family":"Bashashati","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology, Department of Internal Medicine, Texas Tech University Health Sciences Center El Paso, El Paso, TX 79905, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Josue","family":"Enriquez","sequence":"additional","affiliation":[{"name":"Graduate School of Biomedical Sciences, Texas Tech University Health Sciences Center El Paso, El Paso, TX 79905, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4870-718X","authenticated-orcid":false,"given":"Munmun","family":"Chattopadhyay","sequence":"additional","affiliation":[{"name":"Center of Emphasis in Diabetes and Metabolism, Department of Molecular and Translational Medicine, Texas Tech University Health Sciences Center El Paso, El Paso, TX 79905, USA"},{"name":"Graduate School of Biomedical Sciences, Texas Tech University Health Sciences Center El Paso, El Paso, TX 79905, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":null,"published-online":{"date-parts":[[2022,7,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1007\/s13300-016-0182-y","article-title":"A Practical Approach to Gastrointestinal Complications of Diabetes","volume":"7","author":"Maisey","year":"2016","journal-title":"Diabetes Ther."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1989","DOI":"10.1001\/archinte.161.16.1989","article-title":"Prevalence of gastrointestinal symptoms associated with diabetes mellitus: A population-based survey of 15,000 adults","volume":"161","author":"Bytzer","year":"2001","journal-title":"Arch. Intern. Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"51","DOI":"10.4239\/wjd.v4.i3.51","article-title":"Gastrointestinal complications of diabetes mellitus","volume":"4","author":"Krishnan","year":"2013","journal-title":"World J. Diabetes"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1016\/j.bpg.2009.02.012","article-title":"Intestinal and anorectal motility and functional disorders","volume":"23","author":"Keller","year":"2009","journal-title":"Best Pract. Res. Clin. Gastroenterol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"15028","DOI":"10.1038\/mtm.2015.28","article-title":"Hepatic insulin gene therapy prevents diabetic enteropathy in STZ-treated CD-1 mice","volume":"2","author":"You","year":"2015","journal-title":"Mol. Ther.-Methods Clin. Dev."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1007\/s11892-006-0073-0","article-title":"An update on autonomic neuropathy affecting the gastrointestinal tract","volume":"6","author":"Phillips","year":"2006","journal-title":"Curr. Diabetes Rep."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1136\/gutjnl-2015-309151","article-title":"The role of mast cells in functional GI disorders","volume":"65","author":"Wouters","year":"2016","journal-title":"Gut"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1045","DOI":"10.1111\/j.1365-2982.2006.00836.x","article-title":"Role of motility in chronic diarrhoea","volume":"18","author":"Spiller","year":"2006","journal-title":"Neurogastroenterol. Motil."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1116","DOI":"10.1111\/bph.13226","article-title":"Role of the endocannabinoid system in diabetes and diabetic complications","volume":"173","author":"Gruden","year":"2016","journal-title":"Br. J. Pharm."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1007\/BF00400569","article-title":"Hyperglycaemia slows gastric emptying in type 1 (insulin-dependent) diabetes mellitus","volume":"33","author":"Fraser","year":"1990","journal-title":"Diabetologia"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1038\/345346a0","article-title":"Nitric oxide as an inhibitory non-adrenergic non-cholinergic neurotransmitter","volume":"345","author":"Bult","year":"1990","journal-title":"Nature"},{"key":"ref_12","first-page":"538","article-title":"Experimental diabetic autonomic neuropathy characterization in streptozotocin-diabetic Sprague-Dawley rats","volume":"49","author":"Schmidt","year":"1983","journal-title":"Lab. Investig."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1597","DOI":"10.1111\/j.1540-8159.1992.tb02939.x","article-title":"Evidence of impaired afferent vagal function in patients with diabetes gastroparesis","volume":"15","author":"Tougas","year":"1992","journal-title":"Pacing Clin. Electrophysiol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1089\/can.2016.0001","article-title":"Endocannabinoids in the Gut","volume":"1","author":"DiPatrizio","year":"2016","journal-title":"Cannabis Cannabinoid Res."},{"key":"ref_15","first-page":"81","article-title":"Cannabinoids and gastrointestinal motility: Animal and human studies","volume":"12","author":"Aviello","year":"2008","journal-title":"Eur. Rev. Med. Pharmacol. Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1140","DOI":"10.1136\/gut.2008.148791","article-title":"Emerging role of cannabinoids in gastrointestinal and liver diseases: Basic and clinical aspects","volume":"57","author":"Izzo","year":"2008","journal-title":"Gut"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1054\/plef.2001.0341","article-title":"Cannabinoid receptors and their ligands","volume":"66","author":"Pertwee","year":"2002","journal-title":"Prostaglandins Leukot. Essent. Fat. Acids"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1159\/000339072","article-title":"The gastrointestinal pharmacology of cannabinoids: Focus on motility","volume":"90","author":"Abalo","year":"2012","journal-title":"Pharmacology"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1335","DOI":"10.1038\/sj.bjp.0705783","article-title":"Involvement of cannabinoid receptors in gut motility and visceral perception","volume":"141","author":"Hornby","year":"2004","journal-title":"Br. J. Pharm."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1382","DOI":"10.2174\/092986710790980023","article-title":"CB(1) cannabinoid receptors and their associated proteins","volume":"17","author":"Howlett","year":"2010","journal-title":"Curr. Med. Chem."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1038\/sj.bjp.0704293","article-title":"Cannabinoid CB1-receptor mediated regulation of gastrointestinal motility in mice in a model of intestinal inflammation","volume":"134","author":"Izzo","year":"2001","journal-title":"Br. J. Pharm."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1136\/gut.48.6.859","article-title":"Cannabinoids and the gastrointestinal tract","volume":"48","author":"Pertwee","year":"2001","journal-title":"Gut"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1111\/nmo.12329","article-title":"Toward modulation of the endocannabinoid system for treatment of gastrointestinal disease: FAAHster but not \u201chigher\u201d","volume":"26","author":"Nasser","year":"2014","journal-title":"Neurogastroenterol. Motil."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"787-e223","DOI":"10.1111\/j.1365-2982.2010.01478.x","article-title":"Differential effects of CB(1) neutral antagonists and inverse agonists on gastrointestinal motility in mice","volume":"22","author":"Storr","year":"2010","journal-title":"Neurogastroenterol. Motil."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"879","DOI":"10.1093\/brain\/awp014","article-title":"Neuroprotective effect of herpes simplex virus-mediated gene transfer of erythropoietin in hyperglycemic dorsal root ganglion neurons","volume":"132","author":"Chattopadhyay","year":"2008","journal-title":"Brain"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"3099","DOI":"10.1111\/bph.13114","article-title":"Inhibiting endocannabinoid biosynthesis: A novel approach to the treatment of constipation","volume":"172","author":"Bashashati","year":"2015","journal-title":"Br. J. Pharm."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1556","DOI":"10.1111\/j.1476-5381.2011.01644.x","article-title":"Inhibiting fatty acid amide hydrolase normalizes endotoxin-induced enhanced gastrointestinal motility in mice","volume":"165","author":"Bashashati","year":"2012","journal-title":"Br. J. Pharm."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2269","DOI":"10.1210\/jc.2017-02811","article-title":"Gastric Emptying Impacts the Timing of Meal Glucose Peak in Subjects With Uncomplicated Type 1 Diabetes","volume":"103","author":"Lupoli","year":"2018","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2248","DOI":"10.1210\/jc.2015-1055","article-title":"Gastric Emptying Is More Rapid in Adolescents With Type 1 Diabetes and Impacts on Postprandial Glycemia","volume":"100","author":"Perano","year":"2015","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"371","DOI":"10.2337\/diacare.24.2.371","article-title":"Relationships of upper gastrointestinal motor and sensory function with glycemic control","volume":"24","author":"Rayner","year":"2001","journal-title":"Diabetes Care"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"620","DOI":"10.1016\/j.disamonth.2005.11.002","article-title":"Gastrointestinal complications of diabetes mellitus","volume":"51","author":"Ebert","year":"2005","journal-title":"Dis. Mon."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2106","DOI":"10.1023\/A:1018830820537","article-title":"Nitric oxide synthase (NOS) expression in the myenteric plexus of streptozotocin-diabetic rats","volume":"42","author":"Wrzos","year":"1997","journal-title":"Dig. Dis. Sci."},{"key":"ref_33","first-page":"89","article-title":"Diabetic autonomic neuropathy of the gastrointestinal tract","volume":"15","author":"Kuznik","year":"2020","journal-title":"Prz. Gastroenterol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"925","DOI":"10.1007\/s00109-008-0359-6","article-title":"Targeting endocannabinoid degradation protects against experimental colitis in mice: Involvement of CB1 and CB2 receptors","volume":"86","author":"Storr","year":"2008","journal-title":"J. Mol. 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