{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T18:15:41Z","timestamp":1788372941630,"version":"build-2803163510"},"reference-count":25,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2022,7,26]],"date-time":"2022-07-26T00:00:00Z","timestamp":1658793600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>Erysipelothrix rhusiopathiae causes swine erysipelas (SE). Sporadic SE outbreaks in Japan are mostly caused by the E. rhusiopathiae serovar 1a variant featured by methionine (M) and isoleucine (I) at amino acid positions 203 and 257 of the surface protective antigen (Spa) A protein (M203\/I257 SpaA-type). To determine if current vaccines are effective against infection with this variant in pigs, one representative inactivated vaccine, SER-ME (containing E. rhusiopathiae serovar 2a), was evaluated. All vaccinated pigs survived without any apparent clinical signs after lethal challenge with the Fujisawa reference strain or the variant. This indicates that the SER-ME vaccine effectively protects pigs against the infection of E. rhusiopathiae M203\/I257 SpaA-type variant. Current vaccines in Japan, including SER-ME, suggest that outbreaks in Japan are unlikely caused by vaccine failure.<\/jats:p>","DOI":"10.3390\/vetsci9080382","type":"journal-article","created":{"date-parts":[[2022,7,29]],"date-time":"2022-07-29T01:41:16Z","timestamp":1659058876000},"page":"382","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["The Swine Erysipelas Vaccine SER-ME Effectively Protects Pigs against Challenge with the Erysipelothrix rhusiopathiae M203\/I257 SpaA-Type Variant"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8457-169X","authenticated-orcid":false,"given":"Misako","family":"Morimoto","sequence":"first","affiliation":[{"name":"Nippon Institute for Biological Science, 9-2221-1 Shin-machi, Ome 198-0024, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atsushi","family":"Kato","sequence":"additional","affiliation":[{"name":"Nippon Institute for Biological Science, 9-2221-1 Shin-machi, Ome 198-0024, Tokyo, Japan"},{"name":"Biomedical Science Association, 2-20-8-3F Kamiosaki, Shinagawa-ku, Tokyo 141-0021, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kotoe","family":"Nogami","sequence":"additional","affiliation":[{"name":"Nippon Institute for Biological Science, 9-2221-1 Shin-machi, Ome 198-0024, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuta","family":"Akaike","sequence":"additional","affiliation":[{"name":"Nippon Institute for Biological Science, 9-2221-1 Shin-machi, Ome 198-0024, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takaaki","family":"Furusawa","sequence":"additional","affiliation":[{"name":"Nippon Institute for Biological Science, 9-2221-1 Shin-machi, Ome 198-0024, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroe","family":"Kojima","sequence":"additional","affiliation":[{"name":"Nippon Institute for Biological Science, 9-2221-1 Shin-machi, Ome 198-0024, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chihiro","family":"Sasakawa","sequence":"additional","affiliation":[{"name":"Nippon Institute for Biological Science, 9-2221-1 Shin-machi, Ome 198-0024, Tokyo, Japan"},{"name":"Medical Mycology Research Center, Chiba University, 1-8-1 Inohana, Chiba 260-8673, Chiba, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Zimmerman, J.J., Karriker, A.L., Ramirez, A., Schwartz, J.K., Stevenson, W.G., and Zhang, J. (2019). Erysipelas. Diseases of Swine, Wiley-Blackwell.","DOI":"10.1002\/9781119350927"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1991","DOI":"10.1128\/CVI.00213-10","article-title":"Immunization with Truncated Recombinant Protein SpaC of Erysipelothrix rhusiopathiae Strain 715 Serovar 18 Confers Protective Immunity against Challenge with Various Serovars","volume":"17","author":"To","year":"2010","journal-title":"Clin. Vaccine Immunol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"174","DOI":"10.3389\/fvets.2020.00174","article-title":"Erysipelothrix Spp.: Past, Present, and Future Directions in Vaccine Research","volume":"7","author":"Opriessnig","year":"2020","journal-title":"Front. Vet. Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"4376","DOI":"10.1128\/IAI.67.9.4376-4382.1999","article-title":"Truncated Surface Protective Antigen (SpaA) of Erysipelothrix rhusiopathiae Serotype 1a Elicits Protection against Challenge with Serotypes 1a and 2b in Pigs","volume":"67","author":"Imada","year":"1999","journal-title":"Infect. Immun."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"8079","DOI":"10.1016\/j.vaccine.2018.10.071","article-title":"Evaluation of the Protective Efficacy of Four Newly Identified Surface Proteins of Erysipelothrix rhusiopathiae","volume":"36","author":"Zhu","year":"2018","journal-title":"Vaccine"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"206","DOI":"10.4014\/jmb.1407.07058","article-title":"Role of Surface Protective Antigen A in the Pathogenesis of Erysipelothrix rhusiopathiae Strain C43065","volume":"25","author":"Borrathybay","year":"2015","journal-title":"J. Microbiol. Biotechnol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1111\/j.1365-2958.1997.mmi494.x","article-title":"Contribution of Novel Choline-Binding Proteins to Adherence, Colonization and Immunogenicity of Streptococcus Pneumoniae","volume":"25","author":"Rosenow","year":"1997","journal-title":"Mol. Microbiol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1006\/mpat.1998.0216","article-title":"Properties of Repeat Domain Found in a Novel Protective Antigen, SpaA, of Erysipelothrix rhusiopathiae","volume":"25","author":"Makino","year":"1998","journal-title":"Microb. Pathog."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1016\/j.micpath.2017.10.020","article-title":"Characterization of Roles of SpaA in Erysipelothrix rhusiopathiae Adhesion to Porcine Endothelial Cells","volume":"113","author":"Zhu","year":"2017","journal-title":"Microb. Pathog."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1128\/CVI.00099-07","article-title":"Genetic and Antigenic Diversity of the Surface Protective Antigen Proteins of Erysipelothrix rhusiopathiae","volume":"14","author":"To","year":"2007","journal-title":"Clin. Vaccine Immunol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2490","DOI":"10.1016\/j.vaccine.2010.01.041","article-title":"Erysipelothrix rhusiopathiae: Association of Spa-Type with Serotype and Role in Protective Immunity","volume":"28","author":"Ingebritson","year":"2010","journal-title":"Vaccine"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1318","DOI":"10.1292\/jvms.17-0255","article-title":"Multiplex PCR Assay for the Simultaneous Detection and Differentiation of Clonal Lineages of Erysipelothrix rhusiopathiae Serovar 1a Strains Currently Circulating in Japan","volume":"79","author":"Shiraiwa","year":"2017","journal-title":"J. Vet. Med. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"949","DOI":"10.1292\/jvms.11-0533","article-title":"Characterization of Erysipelothrix rhusiopathiae Strains Isolated from Recent Swine Erysipelas Outbreaks in Japan","volume":"74","author":"To","year":"2012","journal-title":"J. Vet. Med. Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1292\/jvms.16-0164","article-title":"Pathogenic Characterization of Erysipelothrix rhusiopathiae Met-203 Type SpaA Strains from Chronic and Subacute Swine Erysipelas in Japan","volume":"79","author":"Uchiyama","year":"2017","journal-title":"J. Vet. Med. Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1007\/s00284-020-02254-2","article-title":"Serovars and SpaA Types of Erysipelothrix rhusiopathiae Isolated from Pigs in Japan from 2012 to 2019","volume":"78","author":"Morimoto","year":"2021","journal-title":"Curr. Microbiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"109458","DOI":"10.1016\/j.vetmic.2022.109458","article-title":"Comparative Study of the Phenotype and Virulence of Recent Serovar 1a, 1b, and 2a Isolates of Erysipelothrix rhusiopathiae in Japan","volume":"270","author":"Morimoto","year":"2022","journal-title":"Vet. Microbiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.biologicals.2013.12.002","article-title":"Prevalence of Met-203 Type SpaA Variant in Erysipelothrix rhusiopathiae Isolates and the Efficacy of Swine Erysipelas Vaccines in Japan","volume":"42","author":"Uchiyama","year":"2014","journal-title":"Biologicals"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2959","DOI":"10.1128\/JB.01500-10","article-title":"The Genome of Erysipelothrix rhusiopathiae, the Causative Agent of Swine Erysipelas, Reveals New Insights into the Evolution of Firmicutes and the Organism\u2019s Intracellular Adaptations","volume":"193","author":"Ogawa","year":"2011","journal-title":"J. Bacteriol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.mimet.2014.09.003","article-title":"Development of an Appropriate PCR System for the Reclassification of Streptococcus Suis","volume":"107","author":"Ishida","year":"2014","journal-title":"J. Microbiol. Methods"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1177\/104063870802000313","article-title":"Differentiation of Erysipelothrix rhusiopathiae Strains by Nucleotide Sequence Analysis of a Hypervariable Region in the SpaA Gene: Discrimination of a Live Vaccine Strain from Field Isolates","volume":"20","author":"Nagai","year":"2008","journal-title":"J. Vet. Diagnostic Investig."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"119","DOI":"10.2754\/avb201382020119","article-title":"Protection of Immunized Mice and Swine to Challenge Exposure with Erysipelothrix rhusiopathiae Strains Obtained from Recent Swine Erysipelas Outbreaks in Japan","volume":"82","author":"To","year":"2013","journal-title":"Acta Vet. Brno"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1292\/jvms1939.41.593","article-title":"Response of Growth Agglutinating Antibody and Protection of Pigs Inoculated with Swine Erysipelas Live Vaccine","volume":"41","author":"Sawada","year":"1979","journal-title":"Nihon Juigaku Zasshi"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1016\/j.vetmic.2009.08.012","article-title":"Erysipelothrix rhusiopathiae","volume":"140","author":"Wang","year":"2010","journal-title":"Vet. Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"536","DOI":"10.1128\/jcm.25.3.536-539.1987","article-title":"Serotype, Antimicrobial Susceptibility, and Pathogenicity of Erysipelothrix rhusiopathiae Isolates from Tonsils of Apparently Healthy Slaughter Pigs","volume":"25","author":"Takahashi","year":"1987","journal-title":"J. Clin. Microbiol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1111\/1348-0421.12736","article-title":"Wild Boars: A Potential Source of Erysipelothrix rhusiopathiae Infection in Japan","volume":"63","author":"Shimoji","year":"2019","journal-title":"Microbiol. Immunol."}],"container-title":["Veterinary Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-7381\/9\/8\/382\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T23:56:43Z","timestamp":1760140603000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-7381\/9\/8\/382"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7,26]]},"references-count":25,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2022,8]]}},"alternative-id":["vetsci9080382"],"URL":"https:\/\/doi.org\/10.3390\/vetsci9080382","relation":{},"ISSN":["2306-7381"],"issn-type":[{"value":"2306-7381","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,7,26]]}}}