{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T23:01:26Z","timestamp":1784674886242,"version":"3.55.0"},"reference-count":48,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2022,7,27]],"date-time":"2022-07-27T00:00:00Z","timestamp":1658880000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>There is a shortage of studies reporting the efficacy of antimicrobial treatment of dogs with atopic dermatitis (AD) and skin infections (SIs). The aim of this study was to evaluate the change in the severity of skin lesions and pruritus, and the overall efficacy of antimicrobial treatment, in dogs with AD and bacterial overgrowth\/infection and\/or Malassezia dermatitis. A total of 20 dogs with AD and SIs were prospectively enrolled (group A) and they were examined before and after the administration of systemic antimicrobials that resulted in the resolution of SIs. In addition, 19 dogs fulfilling the same inclusion criteria and treated with systemic, with or without topical antimicrobials, were included retrospectively (group B). Since there were no major differences between the groups, their results were combined. The severity of skin lesions decreased significantly, by 30% based on Canine Atopic Dermatitis Extent and Severity Index-4 (CADESI-4), by 28.1% based on the erythema domain of CADESI-4 and based on owner\u2019s global assessment of the severity of skin lesions. Pruritus decreased significantly, by 34.7% based on the Pruritus Visual Analogue Scale (PVAS). The efficacy of antimicrobial treatment was assessed as good to excellent by the investigator and the owner in 55% and 60% of the dogs, respectively. Despite the significant improvement, there was high variability in the response to treatment among dogs. Further studies are needed to find factors that determine the response to antimicrobial treatment in dogs with AD and SIs.<\/jats:p>","DOI":"10.3390\/vetsci9080385","type":"journal-article","created":{"date-parts":[[2022,7,29]],"date-time":"2022-07-29T01:41:16Z","timestamp":1659058876000},"page":"385","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Efficacy of Antimicrobial Treatment in Dogs with Atopic Dermatitis: An Observational Study"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8600-8385","authenticated-orcid":false,"given":"Evi I.","family":"Sofou","sequence":"first","affiliation":[{"name":"Clinic of Medicine, Faculty of Veterinary Science, University of Thessaly, Trikalon Str. 224, GR-43132 Karditsa, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Svetlina","family":"Aleksandrova","sequence":"additional","affiliation":[{"name":"Clinic of Medicine, Faculty of Veterinary Science, University of Thessaly, Trikalon Str. 224, GR-43132 Karditsa, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisa","family":"Badulescu","sequence":"additional","affiliation":[{"name":"Clinic of Medicine, Faculty of Veterinary Science, University of Thessaly, Trikalon Str. 224, GR-43132 Karditsa, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manolis","family":"Chatzis","sequence":"additional","affiliation":[{"name":"Clinic of Medicine, Faculty of Veterinary Science, University of Thessaly, Trikalon Str. 224, GR-43132 Karditsa, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8308-3371","authenticated-orcid":false,"given":"Manolis","family":"Saridomichelakis","sequence":"additional","affiliation":[{"name":"Clinic of Medicine, Faculty of Veterinary Science, University of Thessaly, Trikalon Str. 224, GR-43132 Karditsa, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/j.vetimm.2006.08.013","article-title":"Revised nomenclature for veterinary allergy","volume":"114","author":"Halliwell","year":"2006","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"84-e25","DOI":"10.1111\/vde.12197","article-title":"Review: Pathogenesis of canine atopic dermatitis: Skin barrier and host-micro-organism interaction","volume":"26","author":"Santoro","year":"2015","journal-title":"Vet. Dermatol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"95-e26","DOI":"10.1111\/vde.12198","article-title":"Review: Role of genetics and the environment in the pathogenesis of canine atopic dermatitis","volume":"26","author":"Bizikova","year":"2015","journal-title":"Vet. Dermatol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"115-e130","DOI":"10.1111\/vde.12198","article-title":"Review: The role of antibodies, autoantigens and food allergens in canine atopic dermatitis","volume":"26","author":"Bizikova","year":"2015","journal-title":"Vet. Dermatol."},{"key":"ref_5","first-page":"124-e132","article-title":"Review: Lymphocytes, cytokines, chemokines and the T-helper 1-T-helper 2 balance in canine atopic dermatitis","volume":"26","author":"Bizikova","year":"2015","journal-title":"Vet. Dermatol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1291","DOI":"10.2460\/javma.254.11.1291","article-title":"Update on pathogenesis, diagnosis, and treatment of atopic dermatitis in dogs","volume":"254","author":"Nuttall","year":"2019","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"547-e151","DOI":"10.1111\/vde.12965","article-title":"Advances in our understanding of canine atopic dermatitis","volume":"32","author":"Marsella","year":"2021","journal-title":"Vet. Dermatol."},{"key":"ref_8","first-page":"60-e15","article-title":"The cutaneous ecosystem: The roles of the skin microbiome in health and its association with inflammatory skin conditions in humans and animals","volume":"28","year":"2017","journal-title":"Vet. Dermatol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1111\/cea.13820","article-title":"Exogenous factors in the pathogenesis of atopic dermatitis: Irritants and cutaneous infections","volume":"51","author":"Yue","year":"2021","journal-title":"Clin. Exp. Allergy"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"fiv139","DOI":"10.1093\/femsec\/fiv139","article-title":"What is living on your dog\u2019s skin? Characterization of the canine cutaneous mycobiota and fungal dysbiosis in canine allergic dermatitis","volume":"91","author":"Diesel","year":"2015","journal-title":"FEMS Microbiol. Ecol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1182","DOI":"10.1016\/j.jid.2016.01.023","article-title":"Longitudinal evaluation of the skin microbiome and association with microenvironment and treatment in canine atopic dermatitis","volume":"136","author":"Bradley","year":"2016","journal-title":"J. Investig. Dermatol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"332-e382","DOI":"10.1111\/vde.12366","article-title":"The skin microbiome in allergen-induced canine atopic dermatitis","volume":"27","author":"Pierezan","year":"2016","journal-title":"Vet. Dermatol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1016\/j.vetmic.2018.12.022","article-title":"The bacterial and fungal microbiome of the skin of healthy dogs and dogs with atopic dermatitis and the impact of topical antimicrobial therapy, an exploratory study","volume":"229","author":"Chermprapai","year":"2019","journal-title":"Vet. Microbiol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"446-e118","DOI":"10.1111\/vde.12894","article-title":"Serum canine thymus and activation-regulated chemokine (TARC\/CCL17) concentrations correlate with disease severity and therapeutic responses in dogs with atopic dermatitis","volume":"31","author":"Asahina","year":"2020","journal-title":"Vet. Dermatol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"756","DOI":"10.1093\/mmy\/myz118","article-title":"Malassezia species dysbiosis in natural and allergen-induced atopic dermatitis in dogs","volume":"58","author":"Olivry","year":"2020","journal-title":"Med. Mycol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/S0165-2427(01)00345-2","article-title":"The ACVD task force on canine atopic dermatitis (XII): The relationship of cutaneous infections to the pathogenesis and clinical course of canine atopic dermatitis","volume":"81","author":"DeBoer","year":"2001","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Older, C.E., Rodrigues Hoffmann, A., Hoover, K., and Banovic, F. (2020). Characterization of cutaneous bacterial microbiota from superficial pyoderma forms in atopic dogs. Pathogens, 9.","DOI":"10.3390\/pathogens9080638"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1016\/S0165-2427(01)00347-6","article-title":"The ACVD task force on canine atopic dermatitis (XIX): General principles of therapy","volume":"81","author":"Olivry","year":"2001","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1046\/j.0959-4493.2001.00261.x","article-title":"Serum antibodies to Malassezia yeasts in canine atopic dermatitis","volume":"12","author":"Nuttall","year":"2001","journal-title":"Vet. Dermatol."},{"key":"ref_20","unstructured":"Noli, C., Foster, A., and Rosenkrantz, W. (2014). Guidelines for symptomatic medical treatment of canine atopic dermatitis. Veterinary Allergy, Wiley Blackwell. [1st ed.]."},{"key":"ref_21","first-page":"153","article-title":"Evaluation of weekly bathing in allergic dogs with methicillin-resistant Staphylococcal colonization","volume":"64","author":"Kawarai","year":"2016","journal-title":"Jpn. J. Vet. Res."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"569-e138","DOI":"10.1111\/vde.12466","article-title":"A double-blinded, randomized, controlled, crossover evaluation of a zinc methionine supplement as an adjunctive treatment for canine atopic dermatitis","volume":"28","author":"McFadden","year":"2017","journal-title":"Vet. Dermatol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"228-e259","DOI":"10.1111\/vde.12948","article-title":"Preliminary evaluation of two bathing methods for the management of Malassezia overgrowth in dogs with atopic dermatitis","volume":"32","author":"Esumi","year":"2021","journal-title":"Vet. Dermatol."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Olivry, T., Bensignor, E., Favrot, C., Griffin, C.E., Hill, P.B., Mueller, R.S., Plant, J.D., Williams, H.C., and for the International Committee of Allergic Diseases of Animals (ICADA) (2018). Development of a core outcome set for therapeutic clinical trials enrolling dogs with atopic dermatitis (COSCAD\u201918). BMC Vet. Res., 14.","DOI":"10.1186\/s12917-018-1569-y"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1111\/j.1365-3164.2009.00758.x","article-title":"A prospective study on the clinical features of chronic canine atopic dermatitis and its diagnosis","volume":"21","author":"Favrot","year":"2010","journal-title":"Vet. Dermatol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1136\/vr.101069","article-title":"Suggested guidelines for using systemic antimicrobials in bacterial skin infections (1): Diagnosis based on clinical presentation, cytology and culture","volume":"172","author":"Beco","year":"2013","journal-title":"Vet. Rec."},{"key":"ref_27","first-page":"27-e24","article-title":"Biology, diagnosis and treatment of Malassezia dermatitis in dogs and cats. Clinical consensus guidelines of the World Association for Veterinary Dermatology","volume":"31","author":"Bond","year":"2020","journal-title":"Vet. Dermatol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"305-e71","DOI":"10.1111\/vde.13076","article-title":"Repeatability and reproducibility of microscopic examination of adhesive tape strip cytology slides for the quantification of Malassezia spp. in canine skin","volume":"33","author":"Tapes","year":"2022","journal-title":"Vet. Dermatol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1111\/vde.12016","article-title":"Evidence-based guidelines for anti-allergic drug withdrawal times before allergen-specific intradermal and IgE serological tests in dogs","volume":"24","author":"Olivry","year":"2013","journal-title":"Vet. Dermatol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1111\/j.1365-3164.2011.00969.x","article-title":"Serum pharmacokinetics of clindamycin hydrochloride in normal dogs when administered at two dosage regimens","volume":"22","author":"Saridomichelakis","year":"2011","journal-title":"Vet. Dermatol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1136\/vr.101070","article-title":"Suggested guidelines for using systemic antimicrobials in bacterial skin infections: Part 2-antimicrobial choice, treatment regimens and compliance","volume":"172","author":"Beco","year":"2013","journal-title":"Vet. Rec."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"163-e143","DOI":"10.1111\/vde.12118","article-title":"Guidelines for the diagnosis and antimicrobial therapy of canine superficial bacterial folliculitis (Antimicrobial Guidelines Working Group of the International Society for Companion Animal Infectious Diseases)","volume":"25","author":"Hillier","year":"2014","journal-title":"Vet. Dermatol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"77-e25","DOI":"10.1111\/vde.12107","article-title":"Validation of the Canine Atopic Dermatitis Extent and Severity Index (CADESI)-4, a simplified severity scale for assessing skin lesions of atopic dermatitis in dogs","volume":"25","author":"Olivry","year":"2014","journal-title":"Vet. Dermatol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1111\/vde.12709","article-title":"Could we use the erythema grading of the CADESI4 as a simple instrument for future short-duration clinical trials of dogs with atopic dermatitis?","volume":"30","author":"Olivry","year":"2019","journal-title":"Vet. Dermatol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"207-e243","DOI":"10.1111\/vde.12824","article-title":"Development and validation of a graphic 2D investigator\u2019s global assessment instrument for grading the overall severity of atopic dermatitis in dogs","volume":"31","author":"High","year":"2020","journal-title":"Vet. Dermatol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1111\/j.1365-3164.2007.00616.x","article-title":"Development of an owner-assesed scale to measure the severity of pruritus in dogs","volume":"18","author":"Hill","year":"2007","journal-title":"Vet. Dermatol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1111\/j.1365-3164.2008.00728.x","article-title":"Further validation of a pruritus severity scale for use in dogs","volume":"20","author":"Harvey","year":"2009","journal-title":"Vet. Dermatol."},{"key":"ref_38","first-page":"116-e120","article-title":"Serum IgE and IgG responses to dietary antigens in dogs with and without cutaneous adverse food reactions","volume":"31","author":"Mougeot","year":"2020","journal-title":"Vet. Dermatol."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Hensel, P., Santoro, D., Favrot, C., Hill, P., and Griffin, C. (2015). Canine atopic dermatitis: Detailed guidelines for diagnosis and allergen identification. BMC Vet. Res., 11.","DOI":"10.1186\/s12917-015-0515-5"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1111\/j.1365-3164.2009.00776.x","article-title":"New diagnostic criteria for canine atopic dermatitis","volume":"21","author":"Olivry","year":"2010","journal-title":"Vet. Dermatol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1111\/j.1365-3164.2010.00925.x","article-title":"Breed-associated phenotypes in canine atopic dermatitis","volume":"22","author":"Wilhem","year":"2011","journal-title":"Vet. Dermatol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"681-e182","DOI":"10.1111\/vde.12943","article-title":"Onset and duration of action of lokivetmab in a canine model of IL-31 induced pruritus","volume":"32","author":"Fleck","year":"2021","journal-title":"Vet. Dermatol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1007\/s13555-019-0299-9","article-title":"Itraconazole in the treatment of nonfungal cutaneous diseases: A review","volume":"9","author":"Tsai","year":"2019","journal-title":"Dermatol. Ther."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1007","DOI":"10.1080\/08820139.2020.1797778","article-title":"A review of immunomodulatory effects of fluoroquinolones","volume":"50","author":"Assar","year":"2021","journal-title":"Immunol. Investig."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1097\/DER.0000000000000589","article-title":"Staphylococcus aureus in atopic dermatitis: Past, present, and future","volume":"31","author":"Hwang","year":"2020","journal-title":"Dermatitis"},{"key":"ref_46","first-page":"111-e118","article-title":"Antibody levels to Malassezia pachydermatis and Staphylococcus pseudintermedius in atopic dogs and their relationship with lesion scores","volume":"31","author":"Khantavee","year":"2019","journal-title":"Vet. Dermatol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"304-e369","DOI":"10.1111\/vde.12444","article-title":"Recommendations for approaches to meticillin-resistant staphylococcal infections of small animals: Diagnosis, therapeutic considerations and preventative measures: Clinical Consensus Guidelines of the World Association for Veterinary Dermatology","volume":"28","author":"Morris","year":"2017","journal-title":"Vet. Dermatol."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Sousa, C.A., Chatfield, J., File, T.M., Koch, S.N., Leu, D.B., Loeffler, A., Loft, K.E., Souza, C., and Weese, J.S. (2022). Hostage to history-questioning the duration of systemic antimicrobial therapy for the treatment of canine superficial bacterial folliculitis. J. Am. Vet. Med. Assoc., 1\u20134.","DOI":"10.2460\/javma.22.03.0113"}],"container-title":["Veterinary Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-7381\/9\/8\/385\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T23:57:09Z","timestamp":1760140629000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-7381\/9\/8\/385"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7,27]]},"references-count":48,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2022,8]]}},"alternative-id":["vetsci9080385"],"URL":"https:\/\/doi.org\/10.3390\/vetsci9080385","relation":{},"ISSN":["2306-7381"],"issn-type":[{"value":"2306-7381","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,7,27]]}}}