{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T01:20:07Z","timestamp":1789435207721,"version":"build-2803163510"},"reference-count":35,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2022,8,31]],"date-time":"2022-08-31T00:00:00Z","timestamp":1661904000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Academician Innovation Platform Project of Hainan Province","award":["YSPTZX202013"],"award-info":[{"award-number":["YSPTZX202013"]}]},{"name":"Academician Innovation Platform Project of Hainan Province","award":["MOF, MOAR-CARS38"],"award-info":[{"award-number":["MOF, MOAR-CARS38"]}]},{"name":"Academician Innovation Platform Project of Hainan Province","award":["ZY2022HN09"],"award-info":[{"award-number":["ZY2022HN09"]}]},{"name":"Hainan Innovation Center for Academician of Jin Ningyi","award":["YSPTZX202013"],"award-info":[{"award-number":["YSPTZX202013"]}]},{"name":"Hainan Innovation Center for Academician of Jin Ningyi","award":["MOF, MOAR-CARS38"],"award-info":[{"award-number":["MOF, MOAR-CARS38"]}]},{"name":"Hainan Innovation Center for Academician of Jin Ningyi","award":["ZY2022HN09"],"award-info":[{"award-number":["ZY2022HN09"]}]},{"name":"Hainan Innovation Center for Academician of Zhang Yong","award":["YSPTZX202013"],"award-info":[{"award-number":["YSPTZX202013"]}]},{"name":"Hainan Innovation Center for Academician of Zhang Yong","award":["MOF, MOAR-CARS38"],"award-info":[{"award-number":["MOF, MOAR-CARS38"]}]},{"name":"Hainan Innovation Center for Academician of Zhang Yong","award":["ZY2022HN09"],"award-info":[{"award-number":["ZY2022HN09"]}]},{"name":"National Modern Agricultural Industrial Technology System Project","award":["YSPTZX202013"],"award-info":[{"award-number":["YSPTZX202013"]}]},{"name":"National Modern Agricultural Industrial Technology System Project","award":["MOF, MOAR-CARS38"],"award-info":[{"award-number":["MOF, MOAR-CARS38"]}]},{"name":"National Modern Agricultural Industrial Technology System Project","award":["ZY2022HN09"],"award-info":[{"award-number":["ZY2022HN09"]}]},{"name":"Special Funding Projects for Local Science and Technology Development Guided by the Central Committee","award":["YSPTZX202013"],"award-info":[{"award-number":["YSPTZX202013"]}]},{"name":"Special Funding Projects for Local Science and Technology Development Guided by the Central Committee","award":["MOF, MOAR-CARS38"],"award-info":[{"award-number":["MOF, MOAR-CARS38"]}]},{"name":"Special Funding Projects for Local Science and Technology Development Guided by the Central Committee","award":["ZY2022HN09"],"award-info":[{"award-number":["ZY2022HN09"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>K. pneumoniae is an opportunistic pathogen that leads to widespread infection in humans and animals, seriously threatening human health and animal husbandry development. In our research, we investigated the biological characteristics of the isolate by using a 16S rRNA gene sequencing, biochemical assay, and drug sensitivity test. Moreover, the pathogenicity study, including the bacteria load determination, the histopathology examination, and the RNA sequencing was carried out to explore whether the isolate could cause lung injury in mice through intraperitoneal injection. The results indicated that the isolate was identified as K. pneumoniae and named as KPHN001. The drug susceptibility test showed that KPHN001 was only sensitive to polymyxin B and furazolidone, and was resistant to other 28 antibiotics. In the bacteria load determination, the highest bacterial load of the organs was found in the spleen, and abundant bacterial colonization was also found in the lung. The histopathology showed the mainly acute inflammations in the lung were due to congestion, edema, and exudation. RNA-seq analysis revealed that the differentially expressed genes (DEGs) of inflammatory cytokines and chemokines were expressed massively in mice. In the present research, the biological characteristics and pathogenesis of clinically isolated K. pneumoniae were systematically studied, revealing the pathogenic mechanism of K. pneumoniae to animals, and providing a theoretical basis for the following prevention, control, and diagnosis research.<\/jats:p>","DOI":"10.3390\/vetsci9090471","type":"journal-article","created":{"date-parts":[[2022,9,1]],"date-time":"2022-09-01T03:55:38Z","timestamp":1662004538000},"page":"471","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Microbiological Characteristics and Pathogenesis of Klebsiella pneumoniae Isolated from Hainan Black Goat"],"prefix":"10.3390","volume":"9","author":[{"given":"Meirong","family":"He","sequence":"first","affiliation":[{"name":"Hainan Key Laboratory of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Animal Genetic Engineering Key Lab of Haikou, College of Animal Science and Technology, Hainan University, Haikou 570228, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haoyang","family":"Li","sequence":"additional","affiliation":[{"name":"Hainan Key Laboratory of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Animal Genetic Engineering Key Lab of Haikou, College of Animal Science and Technology, Hainan University, Haikou 570228, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenxing","family":"Zhang","sequence":"additional","affiliation":[{"name":"Hainan Key Laboratory of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Animal Genetic Engineering Key Lab of Haikou, College of Animal Science and Technology, Hainan University, Haikou 570228, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junming","family":"Jiang","sequence":"additional","affiliation":[{"name":"Hainan Key Laboratory of Tropical Animal Reproduction & Breeding and Epidemic 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