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The low efficiency of in vitro-produced embryos to derive embryonic stem cells has made achieving this goal a challenge. The fallopian tube plays an important role in the development of embryos. Extracellular vesicles (EVs) secreted by oviductal epithelial cells play an important role in the epigenetic regulation of embryo development. We used artificially isolated oviductal epithelial cells and EVs. In this study, oviductal epithelial cell (OEC) EVs were isolated and characterized through transmission electron microscopy, nanoparticles tracking analysis, western blotting and proteomics. We found that embryo development and blastocyst formation rate was significantly increased (14.3% \u00b1 0.6% vs. 6.0% \u00b1 0.6%) after OEC EVs treatment. According to our data, the inner cell mass (ICM)\/trophectoderm (TE) ratio of the embryonic cell number increased significantly after OEC EVs treatment (43.7% \u00b1 2.3% vs. 28.4% \u00b1 2.1%). Meanwhile, the attachment ability of embryos treated with OEV EVs was significantly improved (43.5% \u00b1 2.1% vs. 29.2% \u00b1 2.5%, respectively). Using quantitative polymerase chain reaction (qPCR), we found that the expression of reprogramming genes (POU5F1, SOX2, NANOG, KLF4 and c-Myc) and implantation-related genes (VIM, KRT8, TEAD4 and CDX2) significantly increased in OEC EV-treated embryos. We report that OEC EV treatment can improve the development and implantation abilities of embryos.<\/jats:p>","DOI":"10.3390\/vetsci9110609","type":"journal-article","created":{"date-parts":[[2022,11,4]],"date-time":"2022-11-04T04:00:51Z","timestamp":1667534451000},"page":"609","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Oviduct Epithelial Cell-Derived Extracellular Vesicles Improve Porcine Trophoblast Outgrowth"],"prefix":"10.3390","volume":"9","author":[{"given":"Xun","family":"Fang","sequence":"first","affiliation":[{"name":"Laboratory of Theriogenology, College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9901-5875","authenticated-orcid":false,"given":"Bereket Molla","family":"Tanga","sequence":"additional","affiliation":[{"name":"Laboratory of Theriogenology, College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seonggyu","family":"Bang","sequence":"additional","affiliation":[{"name":"Laboratory of Theriogenology, College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chaerim","family":"Seo","sequence":"additional","affiliation":[{"name":"Laboratory of Theriogenology, College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heyyoung","family":"Kim","sequence":"additional","affiliation":[{"name":"School of Biological Sciences and Technology, College of Natural Sciences, Chonnam National University, Gwangju 34134, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7633-730X","authenticated-orcid":false,"given":"Islam M.","family":"Saadeldin","sequence":"additional","affiliation":[{"name":"Laboratory of Theriogenology, College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Korea"},{"name":"Research Institute of Veterinary Medicine, Chungnam National University, Daejeon 34134, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1265-3821","authenticated-orcid":false,"given":"Sanghoon","family":"Lee","sequence":"additional","affiliation":[{"name":"Laboratory of Theriogenology, College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8431-0457","authenticated-orcid":false,"given":"Jongki","family":"Cho","sequence":"additional","affiliation":[{"name":"Laboratory of Theriogenology, College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1016\/j.theriogenology.2016.04.030","article-title":"Derivation of porcine pluripotent stem cells for biomedical research","volume":"86","author":"Shiue","year":"2016","journal-title":"Theriogenology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1146\/annurev-animal-021815-111202","article-title":"Pluripotent Stem Cells from Domesticated Mammals","volume":"4","author":"Ezashi","year":"2016","journal-title":"Annu. 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