{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T12:14:19Z","timestamp":1785413659262,"version":"3.56.0"},"reference-count":33,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T00:00:00Z","timestamp":1778198400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Portuguese Foundation for Science and Technology","award":["UID\/04650\/2025"],"award-info":[{"award-number":["UID\/04650\/2025"]}]},{"award":["UID\/04650\/2025"],"award-info":[{"award-number":["UID\/04650\/2025"]}],"id":[{"id":"https:\/\/ror.org\/00snfqn58","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vision"],"abstract":"<jats:p>Background: Low-level red-light therapy (RLRT) has emerged as a potential therapy in myopia management. However, its effects on retinal structure and function following repeated exposure remain incompletely understood. Purpose: To evaluate the short-term effects of RLRT on central retinal function in myopes and non-myopes using retinal electrophysiology and structural parameters. Methods: Thirty-six subjects underwent RLRT exposures. Retinal function was assessed using pattern electroretinography (PERG) and photopic full-field electroretinography (Photopic 3.0 ERG). Structural measurements, including axial length (AL), obtained with an optical biometer and choroidal thickness (CT), were measured using MOPTIM 3000. Outcomes were compared between baseline and post-RLRT conditions (single 3 min and single 1 min exposure), across refractive groups, and correlated with AL and CT. This study was conducted at the University of Minho in accordance with the Declaration of Helsinki. Results: In myopic subjects, PERG N35\u2013P50 amplitude significantly increased after 3 min of RLRT (baseline:0.88 \u00b1 0.21 \u00b5V; post: 1.40 \u00b1 0.25 \u00b5V; p = 0.013), whereas no significant changes were observed in non-myopes (baseline: 1.32 \u00b1 0.19 \u00b5V; post: 1.39 \u00b1 0.21 \u00b5V; p = 0.47). Latencies remained stable across all groups (p &gt; 0.05). No significant correlations were found between PERG and AL (\u03c1 = \u22120.18, p = 0.44) or CT (\u03c1 = 0.12, p = 0.52). Photopic 3.0 ERG showed an increase in b-wave amplitude after 3 min of RLRT in myopes (\u0394 = +2.42 \u00b5V, p = 0.06), but not in non-myopes. In myopes, AL was negatively correlated with post-therapy latency (\u03c1 = \u22120.624, p = 0.060), while CT showed strong correlations with photopic a-wave responses after 1 min RLRT (CT vs. latency: \u03c1 = \u22120.873, p = 0.010; CT vs. amplitude: \u03c1 = 0.821, p = 0.034). Conclusions: Short-term exposure to RLRT, particularly at 3 min, enhances PERG and Photopic ERG responses in myopic subjects, suggesting transient improvements in retinal function with differential effects between refractive groups.<\/jats:p>","DOI":"10.3390\/vision10020026","type":"journal-article","created":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T19:35:43Z","timestamp":1778528143000},"page":"26","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Short-Term Effects of Low-Level Red-Light Therapy on Central Retinal Function: A Combined Pattern ERG and Photopic ERG Study"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5730-8722","authenticated-orcid":false,"given":"Muhammad","family":"Qasim","sequence":"first","affiliation":[{"name":"Physics Center for Universities of Minho and Porto (CF-UM-UP), School of Sciences, Gualtar Campus, University of Minho, 4710-057 Braga, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3921-6357","authenticated-orcid":false,"given":"Paulo","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Physics Center for Universities of Minho and Porto (CF-UM-UP), School of Sciences, Gualtar Campus, University of Minho, 4710-057 Braga, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4369-3332","authenticated-orcid":false,"given":"Jorge","family":"Jorge","sequence":"additional","affiliation":[{"name":"Physics Center for Universities of Minho and Porto (CF-UM-UP), School of Sciences, Gualtar Campus, University of Minho, 4710-057 Braga, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,5,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s10633-012-9353-y","article-title":"ISCEV standard for clinical pattern electroretinography (PERG): 2012 update","volume":"126","author":"Bach","year":"2013","journal-title":"Doc. Ophthalmol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s10633-014-9473-7","article-title":"ISCEV Standard for full-field clinical electroretinography (2015 update)","volume":"130","author":"McCulloch","year":"2015","journal-title":"Doc. Ophthalmol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1007\/s10633-024-09970-1","article-title":"ISCEV standard for clinical pattern electroretinography (2024 update)","volume":"148","author":"Thompson","year":"2024","journal-title":"Doc. Ophthalmol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1007\/s10633-018-9645-y","article-title":"ISCEV extended protocol for the photopic On-Off ERG","volume":"136","author":"Sustar","year":"2018","journal-title":"Doc. Ophthalmol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/j.ajo.2024.10.011","article-title":"Axial Shortening Effects of Repeated Low-level Red-light Therapy in Children with High Myopia: A Multicenter Randomized Controlled Trial","volume":"270","author":"Liu","year":"2025","journal-title":"Am. J. Ophthalmol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1016\/j.ophtha.2021.11.023","article-title":"Effect of Repeated Low-Level Red-Light Therapy for Myopia Control in Children: A Multicenter Randomized Controlled Trial","volume":"129","author":"Jiang","year":"2022","journal-title":"Ophthalmology"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1186\/s12967-025-07514-y","article-title":"The effectiveness of red-light therapy on myopia control depends on its direct effect: A mediation analysis","volume":"24","author":"Chen","year":"2025","journal-title":"J. Transl. Med."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1097\/ICL.0000000000001020","article-title":"Repeated Low-Level Red Light Therapy for the Control of Myopia in Children: A Meta-Analysis of Randomized Controlled Trials","volume":"49","author":"Wang","year":"2023","journal-title":"Eye Contact Lens"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1097\/IAE.0000000000003980","article-title":"LIGHTSITE III: 13-Month Efficacy and Safety Evaluation of Multiwavelength Photobiomodulation in Nonexudative (Dry) Age-Related Macular Degeneration Using the Lumithera Valeda Light Delivery System","volume":"44","author":"Boyer","year":"2024","journal-title":"Retina"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"953","DOI":"10.1007\/s40123-022-00640-6","article-title":"LIGHTSITE II Randomized Multicenter Trial: Evaluation of Multiwavelength Photobiomodulation in Non-exudative Age-Related Macular Degeneration","volume":"12","author":"Burton","year":"2023","journal-title":"Ophthalmol. Ther."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"206","DOI":"10.11648\/j.ajbls.20200806.13","article-title":"Evaluation of the Function of Ganglion Cells in Myopic Patients Using Pattern Electroretinogram (PERG)","volume":"8","author":"Nasser","year":"2020","journal-title":"Am. J. Biomed. Life Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/S0002-9394(14)74550-7","article-title":"The pattern electroretinogram in diabetes","volume":"109","author":"Prager","year":"1990","journal-title":"Am. J. Ophthalmol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1016\/S1350-9462(00)00030-6","article-title":"Pattern electroretinography (PERG) and an integrated approach to visual pathway diagnosis","volume":"20","author":"Holder","year":"2001","journal-title":"Prog. Retin. Eye Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"386","DOI":"10.1097\/OPX.0b013e318177ebf3","article-title":"Update on the pattern electroretinogram in glaucoma","volume":"85","author":"Bach","year":"2008","journal-title":"Optom. Vis. Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"5636","DOI":"10.1167\/iovs.08-1946","article-title":"Photopic negative response of focal electoretinograms in glaucomatous eyes","volume":"49","author":"Machida","year":"2008","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2406","DOI":"10.1038\/s41433-021-01659-y","article-title":"The electroretinogram in the genomics era: Outer retinal disorders","volume":"35","author":"Cornish","year":"2021","journal-title":"Eye"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1007\/s10633-021-09857-5","article-title":"Electroretinogram responses in myopia: A review","volume":"145","author":"Gupta","year":"2022","journal-title":"Doc. Ophthalmol."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Tokoro, T. (1998). Electrophysiological Changes in Photopic Function in Myopia. Myopia Updates, Springer.","DOI":"10.1007\/978-4-431-66959-3"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Zahra, S., Murphy, M.J., Crewther, S.G., and Riddell, N. (2023). Flash Electroretinography as a Measure of Retinal Function in Myopia and Hyperopia: A Systematic Review. Vision, 7.","DOI":"10.3390\/vision7010015"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1016\/j.exer.2015.05.008","article-title":"Electrophysiological assessment of retinal ganglion cell function","volume":"141","author":"Porciatti","year":"2015","journal-title":"Exp. Eye Res."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"2392","DOI":"10.1038\/s41433-024-03154-6","article-title":"Electrodiagnostic tests of the visual pathway and applications in neuro-ophthalmology","volume":"38","author":"Calcagni","year":"2024","journal-title":"Eye"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1097\/01.APO.0000578944.25956.8b","article-title":"Myopia: Anatomic Changes and Consequences for Its Etiology","volume":"8","author":"Jonas","year":"2019","journal-title":"Asia Pac. J. Ophthalmol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1739","DOI":"10.1016\/S0140-6736(12)60272-4","article-title":"Myopia","volume":"379","author":"Morgan","year":"2012","journal-title":"Lancet"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1097\/ICL.0b013e3181b343d9","article-title":"Pattern electroretinographic recordings in eyes with myopia","volume":"35","author":"Oner","year":"2009","journal-title":"Eye Contact Lens"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1016\/j.preteyeres.2009.12.002","article-title":"The multifunctional choroid","volume":"29","author":"Nickla","year":"2010","journal-title":"Prog. Retin. Eye Res."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1363","DOI":"10.7150\/ijms.85746","article-title":"Repeated Low-Level Red-Light Therapy for Con-trolling Onset and Progression of Myopia-a Review","volume":"20","author":"Zhu","year":"2023","journal-title":"Int. J. Med. Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"337","DOI":"10.3934\/biophy.2017.3.337","article-title":"Mechanisms and applications of the anti-inflammatory effects of photobiomodulation","volume":"4","author":"Hamblin","year":"2017","journal-title":"AIMS Biophys."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1007\/s40123-023-00875-x","article-title":"The Effects of Repeated Low-Level Red-Light Therapy on the Structure and Vasculature of the Choroid and Retina in Children with Premyopia","volume":"13","author":"Liu","year":"2024","journal-title":"Ophthalmol. Ther."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.ajo.2013.08.010","article-title":"Epidemiology and disease burden of pathologic myopia and myopic choroidal neovascularization: An evidence-based systematic review","volume":"157","author":"Wong","year":"2014","journal-title":"Am. J. Ophthalmol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1007\/s10633-022-09872-0","article-title":"ISCEV Standard for full-field clinical electroretinography (2022 update)","volume":"144","author":"Robson","year":"2022","journal-title":"Doc. Ophthalmol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1122","DOI":"10.1002\/jbio.201670113","article-title":"Photobiomodulation or low-level laser therapy","volume":"9","author":"Hamblin","year":"2016","journal-title":"J. Biophotonics"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Lin, L.-Y., Hsu, C.-H., Su, H., Sun, J., Wu, J., Jeng, W., Tsai, C., Yuliani, F.S., and Lin, S. (2026). Duration-Dependent Efficacy and Clinical Safety of Repeated Low-Level Red-Light Therapy for Paediatric Myopia: A Systematic Review and Meta-Analysis. Clin. Exp. Ophthalmol., Epub ahead of printing.","DOI":"10.1111\/ceo.70105"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1001\/jamaophthalmol.2025.0835","article-title":"Cone Density Changes After Repeated Low-Level Red Light Treatment in Children with Myopia","volume":"143","author":"Liao","year":"2025","journal-title":"JAMA Ophthalmol."}],"container-title":["Vision"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2411-5150\/10\/2\/26\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T09:33:38Z","timestamp":1778837618000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2411-5150\/10\/2\/26"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,8]]},"references-count":33,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2026,6]]}},"alternative-id":["vision10020026"],"URL":"https:\/\/doi.org\/10.3390\/vision10020026","relation":{},"ISSN":["2411-5150"],"issn-type":[{"value":"2411-5150","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,8]]}}}