{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T02:00:42Z","timestamp":1773194442976,"version":"3.50.1"},"reference-count":46,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2023,4,17]],"date-time":"2023-04-17T00:00:00Z","timestamp":1681689600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Portuguese Foundation for Science and Technology (FCT)","award":["UIDB\/CVT\/00772\/2020"],"award-info":[{"award-number":["UIDB\/CVT\/00772\/2020"]}]},{"name":"Portuguese Foundation for Science and Technology (FCT)","award":["LA\/P\/0059\/2020"],"award-info":[{"award-number":["LA\/P\/0059\/2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Agriculture"],"abstract":"<jats:p>Microbial water quality is a major concern in the world, since the ingestion of water contaminated with microorganisms poses risks to human and animal health. The aim of this study was to evaluate the microbiological quality of drinking water on dairy cattle farms. The study was carried out to determine the occurrence of coliforms and enterococcus species in drinking water samples obtained from selected dairy cattle farms located in four different areas of Portugal (north, central, south and islands). A questionnaire was used to collect relevant information regarding farmer demographics, characteristics of the cattle farms, as well as number of animals per herd, number of adult cows, production type, water source, use of disinfectants and pasture area. In this study, a convenience sample of 32 dairy cattle farms was subjected to screening for water quality. The numbers of animals in the study were 8086 cattle, with a mean herd size of 253, of which 51.7% were cows. Coliform and enterococcus bacterial species were identified by performing the membrane filtration method. The occurrence of poor water quality was recorded in 19 (59.4%) cattle farms. Isolated bacteria included Escherichia coli (20.0%), Enterococcus faecalis (25.0%), total coliforms (65.6%) and fecal coliforms (43.8%). Farms that did not routinely perform disinfection had the worst water quality (70.8%) when compared with farms that performed disinfection (25.0%) (p = 0.022). This research revealed the importance of screening the quality of drinking water on cattle farms, which could contribute to improved animal, human and environmental health in a One Heath approach.<\/jats:p>","DOI":"10.3390\/agriculture13040885","type":"journal-article","created":{"date-parts":[[2023,4,18]],"date-time":"2023-04-18T02:30:03Z","timestamp":1681785003000},"page":"885","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Occurrence of Coliforms and Enterococcus Species in Drinking Water Samples Obtained from Selected Dairy Cattle Farms in Portugal"],"prefix":"10.3390","volume":"13","author":[{"given":"Ana Sofia","family":"Soares","sequence":"first","affiliation":[{"name":"CECAV\u2013Animal and Veterinary Research Centre, University of Tr\u00e1s-os-Montes e Alto Douro (UTAD), 5000-801 Vila Real, Portugal"},{"name":"Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), 5000-801 Vila Real, Portugal"}]},{"given":"Carla","family":"Miranda","sequence":"additional","affiliation":[{"name":"Department of Sciences, University Institute of Health Sciences (IUCS), CESPU, CRL, 4585-116 Gandra, Portugal"},{"name":"Toxicology Research Unit (TOXRUN), University Institute of Health Sciences (IUCS), CESPU, CRL, 4585-116 Gandra, Portugal"},{"name":"Associated Laboratory for Green Chemistry (LAQVREQUIMTE), University NOVA of Lisbon, 2829-516 Caparica, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7196-4179","authenticated-orcid":false,"given":"Ana Claudia","family":"Coelho","sequence":"additional","affiliation":[{"name":"CECAV\u2013Animal and Veterinary Research Centre, University of Tr\u00e1s-os-Montes e Alto Douro (UTAD), 5000-801 Vila Real, Portugal"},{"name":"Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), 5000-801 Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8208-6204","authenticated-orcid":false,"given":"Henrique","family":"Trindade","sequence":"additional","affiliation":[{"name":"Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), UTAD, 5000-801 Vila Real, Portugal"},{"name":"Inov4Agro\u2014Institute for Innovation, Capacity Building and Sustainability of Agri-food Production, 5000-801 Vila Real, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"931","DOI":"10.2134\/jeq2018.07.0277","article-title":"Microbial Water Quality: Monitoring and Modeling","volume":"47","author":"Pachepsky","year":"2018","journal-title":"J. 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