{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T12:44:40Z","timestamp":1763729080407,"version":"build-2065373602"},"reference-count":47,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2025,9,9]],"date-time":"2025-09-09T00:00:00Z","timestamp":1757376000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Union\u2019s Horizon 2020 research and innovation project TRUE","award":["727973","101000622","(MICIU\/AEI\/10.13039\/501100011033)","101135512","10.54499\/UI\/BD\/151389\/2021","UIDB\/50016\/2020"],"award-info":[{"award-number":["727973","101000622","(MICIU\/AEI\/10.13039\/501100011033)","101135512","10.54499\/UI\/BD\/151389\/2021","UIDB\/50016\/2020"]}]},{"name":"European Project Realising Dynamic Value Chains for Underutilised Crops (RADIANT)","award":["727973","101000622","(MICIU\/AEI\/10.13039\/501100011033)","101135512","10.54499\/UI\/BD\/151389\/2021","UIDB\/50016\/2020"],"award-info":[{"award-number":["727973","101000622","(MICIU\/AEI\/10.13039\/501100011033)","101135512","10.54499\/UI\/BD\/151389\/2021","UIDB\/50016\/2020"]}]},{"name":"the Spanish project CPP2022-009742","award":["727973","101000622","(MICIU\/AEI\/10.13039\/501100011033)","101135512","10.54499\/UI\/BD\/151389\/2021","UIDB\/50016\/2020"],"award-info":[{"award-number":["727973","101000622","(MICIU\/AEI\/10.13039\/501100011033)","101135512","10.54499\/UI\/BD\/151389\/2021","UIDB\/50016\/2020"]}]},{"name":"the project LegumES","award":["727973","101000622","(MICIU\/AEI\/10.13039\/501100011033)","101135512","10.54499\/UI\/BD\/151389\/2021","UIDB\/50016\/2020"],"award-info":[{"award-number":["727973","101000622","(MICIU\/AEI\/10.13039\/501100011033)","101135512","10.54499\/UI\/BD\/151389\/2021","UIDB\/50016\/2020"]}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia (FCT) for the Ph.D. scholarship","doi-asserted-by":"publisher","award":["727973","101000622","(MICIU\/AEI\/10.13039\/501100011033)","101135512","10.54499\/UI\/BD\/151389\/2021","UIDB\/50016\/2020"],"award-info":[{"award-number":["727973","101000622","(MICIU\/AEI\/10.13039\/501100011033)","101135512","10.54499\/UI\/BD\/151389\/2021","UIDB\/50016\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Centre for Biotechnology and Fine Chemistry (CBQF)","award":["727973","101000622","(MICIU\/AEI\/10.13039\/501100011033)","101135512","10.54499\/UI\/BD\/151389\/2021","UIDB\/50016\/2020"],"award-info":[{"award-number":["727973","101000622","(MICIU\/AEI\/10.13039\/501100011033)","101135512","10.54499\/UI\/BD\/151389\/2021","UIDB\/50016\/2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Agriculture"],"abstract":"<jats:p>Legumes contribute to sustainable agriculture by reducing fertilizer use, enhancing nitrogen fixation, and with high species diversity (~20,000 species). Spain is a leading EU producer, yielding up to 30,000 tons of different legume varieties annually. The Mediterranean climate, particularly in regions like Andalusia, is under increasing pressure from climate change, with extreme temperature variations and drought becoming more frequent. While these changes may jeopardize crop yields, limited information is available on their effects on the nutritional profile of legumes. From 2017 to 2019, six faba bean (Vicia faba) varieties were monitored in two climatically distinct areas of Andalusia to assess the impact of temperature (T) and rainfall (R) on key nutrients and bioactive compounds, including protein, minerals (K, Ca, Mg, Zn, P, Fe, Mn, B), total polyphenol content (TPC), tannins (TA), and saponins (S). Spearman correlations showed that higher T negatively impacted TPC (r = \u22120.40) and Mg (r = \u22120.33), while positively influencing Zn (r = 0.27) and Ca (r = 0.22). Rainfall increased TPC and Mg but reduced TA, Zn, and Ca. Canonical correspondence analysis (CCA) and PERMANOVA (p &lt; 0.001) confirmed T, R, and yield as significant factors. These insights support breeding strategies for climate-adapted, nutrient-rich faba beans and the development of more resilient food systems.<\/jats:p>","DOI":"10.3390\/agriculture15181909","type":"journal-article","created":{"date-parts":[[2025,9,9]],"date-time":"2025-09-09T08:40:15Z","timestamp":1757407215000},"page":"1909","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Establishing the Effects of Climate and Soil on the Nutritional Composition of an Array of Faba Bean Varieties Grown in Two Different Zones of Andalusia, Spain"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9155-3062","authenticated-orcid":false,"given":"Jazm\u00edn","family":"Osorio","sequence":"first","affiliation":[{"name":"CBQF\u2014Centro de Biotecnologia e Qu\u00edmica Fina-Laborat\u00f3rio Associado, Escola Superior de Biotecnologia, Universidade Cat\u00f3lica Portuguesa, Rua de Diogo Botelho 1327, 4169-005 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4232-8250","authenticated-orcid":false,"given":"Gianuario","family":"Fortunato","sequence":"additional","affiliation":[{"name":"CBQF\u2014Centro de Biotecnologia e Qu\u00edmica Fina-Laborat\u00f3rio Associado, Escola Superior de Biotecnologia, Universidade Cat\u00f3lica Portuguesa, Rua de Diogo Botelho 1327, 4169-005 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6234-6312","authenticated-orcid":false,"given":"Eleonora","family":"Barilli","sequence":"additional","affiliation":[{"name":"Institute for Sustainable Agriculture, CSIC, 14004 C\u00f3rdoba, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9644-8616","authenticated-orcid":false,"given":"Diego","family":"Rubiales","sequence":"additional","affiliation":[{"name":"Institute for Sustainable Agriculture, CSIC, 14004 C\u00f3rdoba, Spain"}]},{"given":"Elisabete","family":"Pinto","sequence":"additional","affiliation":[{"name":"CBQF\u2014Centro de Biotecnologia e Qu\u00edmica Fina-Laborat\u00f3rio Associado, Escola Superior de Biotecnologia, Universidade Cat\u00f3lica Portuguesa, Rua de Diogo Botelho 1327, 4169-005 Porto, Portugal"},{"name":"EPIUnit\u2014Instituto de Sa\u00fade P\u00fablica, Universidade do Porto, 4050-091 Porto, Portugal"}]},{"given":"Marta W.","family":"Vasconcelos","sequence":"additional","affiliation":[{"name":"CBQF\u2014Centro de Biotecnologia e Qu\u00edmica Fina-Laborat\u00f3rio Associado, Escola Superior de Biotecnologia, Universidade Cat\u00f3lica Portuguesa, Rua de Diogo Botelho 1327, 4169-005 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2025,9,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1111\/nbu.12613","article-title":"Impact of a daily legume-based meal on dietary and nutritional intake in a group of omnivorous adults","volume":"48","author":"Ferreira","year":"2023","journal-title":"Nutr. 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Crop Physiology Case Histories for Major Crops, Academic Press.","key":"ref_4","DOI":"10.1016\/B978-0-12-819194-1.00015-3"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"100303","DOI":"10.1016\/j.fochx.2022.100303","article-title":"Nutritional quality of protein flours of fava bean (Vicia faba L.) and in vitro digestibility and bioaccesibility","volume":"14","year":"2022","journal-title":"Food Chem. X"},{"doi-asserted-by":"crossref","unstructured":"Mitchell, D.C., Marinangeli, C.P.F., Pigat, S., Bompola, F., Campbell, J., Pan, Y., Curran, J.M., Cai, D.J., Jaconis, S.Y., and Rumney, J. (2021). Pulse Intake Improves Nutrient Density among US Adult Consumers. Nutrients, 13.","key":"ref_6","DOI":"10.3390\/nu13082668"},{"unstructured":"FAOSTAT (2025, January 29). FAOSTAT Database: Crops and Livestock Products. Available online: https:\/\/www.fao.org\/faostat\/en\/#data\/QCL.","key":"ref_7"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"782574","DOI":"10.3389\/fpls.2021.782574","article-title":"Legume Breeding for the Agroecological Transition of Global Agri-Food Systems: A European Perspective","volume":"12","author":"Rubiales","year":"2021","journal-title":"Front. Plant Sci."},{"unstructured":"Kezeya, B., Muel, F., and Mergenthaler, M. (2025, March 27). The Market of Grain Legumes in Spain: Results of the EU-Project LegValue. Available online: https:\/\/www.legumehub.eu\/is_article\/the-market-of-grain-legumes-in-spain\/.","key":"ref_9"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"e12848","DOI":"10.7717\/peerj.12848","article-title":"Soil health and nutrient density: Preliminary comparison of regenerative and conventional farming","volume":"10","author":"Montgomery","year":"2022","journal-title":"PeerJ"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/bs.agron.2020.06.004","article-title":"Factors influencing elemental micronutrient supply from pasture systems for grazing ruminants","volume":"164","author":"Kao","year":"2020","journal-title":"Adv. Agron."},{"doi-asserted-by":"crossref","unstructured":"Dancausa Mill\u00e1n, M.G., and Mill\u00e1n V\u00e1zquez de la Torre, M.G. (2022). Quality Food Products as a Tourist Attraction in the Province of C\u00f3rdoba (Spain). Int. J. Environ. Res. Public Health, 19.","key":"ref_12","DOI":"10.3390\/ijerph191912754"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"544","DOI":"10.3389\/fpls.2020.00544","article-title":"Root Growth Adaptation to Climate Change in Crops","volume":"11","author":"Boter","year":"2020","journal-title":"Front. Plant Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"034007","DOI":"10.1088\/1748-9326\/3\/3\/034007","article-title":"Why are agricultural impacts of climate change so uncertain? The importance of temperature relative to precipitation","volume":"3","author":"Lobell","year":"2008","journal-title":"Environ. Res. Lett."},{"doi-asserted-by":"crossref","unstructured":"Barilli, E., Luna, P., Flores, F., and Rubiales, D. (2025). Agronomic Performance of Faba Bean in Mediterranean Environments. Agronomy, 15.","key":"ref_15","DOI":"10.3390\/agronomy15020412"},{"unstructured":"(2025, August 20). Saskatchewan Pulse Growers: Faba Beans Seeding. Available online: https:\/\/saskpulse.com\/growing-pulses\/faba-beans\/faba-beans-seeding\/.","key":"ref_16"},{"unstructured":"Ministerio de Agricultura, Pesca y Alimentaci\u00f3n (2025, July 08). Oficina Espa\u00f1ola de Variedades Vegetales, Cat\u00e1logos Nacionales y Comunitarios. Available online: https:\/\/servicio.mapa.gob.es\/germenwai\/BusRegVar.aspx?id=es.","key":"ref_17"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal Biochem."},{"key":"ref_19","first-page":"255","article-title":"Total phenolics and flavonoids in Bulgarian fruits and vegetables","volume":"40","author":"Marinova","year":"2005","journal-title":"J. Univ. Chem. Technol. Metall."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"949","DOI":"10.1007\/s10722-007-9302-x","article-title":"Contents of total protein, L-canavanine and condensed tannins of the one-flowered vetch (Vicia articulata Hornem.) collection of the Bank of Plant Germplasm of Cuenca (Spain)","volume":"55","year":"2008","journal-title":"Genet. Resour. Crop. Evol."},{"unstructured":"(2025, April 01). R Core Team: R: A Language and Environment for Statistical Computing. Available online: https:\/\/www.R-project.org\/.","key":"ref_21"},{"unstructured":"Wei, T., and Simko, V. (2025, April 23). R Package \u201cCorrplot\u201d: Visualization of a Correlation Matrix, Available online: https:\/\/github.com\/taiyun\/corrplot.","key":"ref_22"},{"doi-asserted-by":"crossref","unstructured":"Wickham, H. (Data Analysis in ggplot2. Use R!, 2016). Data Analysis in ggplot2. Use R!, Version 3.5.2.","key":"ref_23","DOI":"10.1007\/978-3-319-24277-4"},{"unstructured":"Oksanen, J., Simpson, G.L., Blanchet, F.G., Kindt, R., Legendre, P., Minchin, P.R., and O\u2019Hara, R.B. (2025, April 23). Vegan: Community Ecology Package. R Package, Available online: https:\/\/cran.rproject.org\/package=vegan.","key":"ref_24"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1033092","DOI":"10.3389\/fpls.2022.1033092","article-title":"Functions and strategies for enhancing zinc availability in plants for sustainable agriculture","volume":"13","author":"Usman","year":"2022","journal-title":"Front. Plant Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1186\/s40066-018-0168-2","article-title":"Yield of faba bean (Vicia faba L.) as affected by lime, mineral P, farmyard manure, compost and rhizobium in acid soil of Lay Gayint District, northwestern highlands of Ethiopia","volume":"7","author":"Fekadu","year":"2018","journal-title":"Agric. Food Secur."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1007\/s10681-018-2154-y","article-title":"Phenotyping of faba beans (Vicia faba L.) under cold and heat stresses using chlorophyll fluorescence","volume":"214","author":"Zhou","year":"2018","journal-title":"Euphytica"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"e08176","DOI":"10.1016\/j.heliyon.2021.e08176","article-title":"Modelling the impacts of climate change on faba bean (Vicia faba L.) production in Welmera area, central Ethiopia","volume":"7","author":"Bogale","year":"2021","journal-title":"Heliyon"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"100240","DOI":"10.1016\/j.fhj.2025.100240","article-title":"Understanding the health impacts of the climate crisis","volume":"12","author":"Maslin","year":"2025","journal-title":"Future Healthc. J."},{"unstructured":"Cedar Lake Ventures, Inc. (2025, April 23). Weather Spark. Available online: https:\/\/weatherspark.com\/h\/m\/35285\/2025\/4\/Historical-Weather-in-April-2025-in-C%C3%B3rdoba-Spain#Figures-Temperature.","key":"ref_30"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1141","DOI":"10.3390\/antiox12061141","article-title":"Antioxidant Activity, Metabolism, and Bioavailability of Polyphenols in the Diet of Animals","volume":"12","author":"Rastija","year":"2023","journal-title":"Antioxidants"},{"doi-asserted-by":"crossref","unstructured":"Laddomada, B., Blanco, A., Mita, G., D\u2019Amico, L., Singh, R.P., Ammar, K., Crossa, J., and Guzm\u00e1n, C. (2021). Drought and Heat Stress Impacts on Phenolic Acids Accumulation in Durum Wheat Cultivars. Foods, 10.","key":"ref_32","DOI":"10.3390\/foods10092142"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"e11193","DOI":"10.7717\/peerj.11193","article-title":"Effect of extreme temperature changes on phenolic, flavonoid contents and antioxidant activity of tomato seedlings (Solanum lycopersicum L.)","volume":"9","author":"Alhaithloul","year":"2021","journal-title":"PeerJ"},{"doi-asserted-by":"crossref","unstructured":"Bian, Z., Wang, Y., Zhang, X., Li, T., Grundy, S., Yang, Q., and Cheng, R. (2020). A Review of Environment Effects on Nitrate Accumulation in Leafy Vegetables Grown in Controlled Environments. Foods, 9.","key":"ref_34","DOI":"10.3390\/foods9060732"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"e14620","DOI":"10.1016\/j.heliyon.2023.e14620","article-title":"Impact of soybean-associated plant growth-promoting bacteria on plant growth modulation under alkaline soil conditions","volume":"9","author":"Roriz","year":"2023","journal-title":"Heliyon"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"930340","DOI":"10.3389\/fpls.2022.930340","article-title":"Overview of biofertilizers in crop production and stress management for sustainable agriculture","volume":"13","author":"Chaudhary","year":"2022","journal-title":"Front. Plant Sci."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.plaphy.2014.10.007","article-title":"Cultivar variability of iron uptake mechanisms in rice (Oryza sativa L.)","volume":"85","author":"Pereira","year":"2014","journal-title":"Plant Physiol. Biochem."},{"doi-asserted-by":"crossref","unstructured":"Montejano-Ram\u00edrez, V., and Valencia-Cantero, E. (2023). Cross-Talk between Iron Deficiency Response and Defense Establishment in Plants. Int. J. Mol. Sci., 24.","key":"ref_38","DOI":"10.3390\/ijms24076236"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1223","DOI":"10.1105\/tpc.001388","article-title":"IRT1, an Arabidopsis Transporter Essential for Iron Uptake from the Soil and for Plant Growth","volume":"14","author":"Vert","year":"2002","journal-title":"Plant Cell."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"01658","DOI":"10.3389\/fpls.2017.01658","article-title":"Food Legumes and Rising Temperatures: Effects, Adaptive Functional Mechanisms Specific to Reproductive Growth Stage and Strategies to Improve Heat Tolerance","volume":"8","author":"Sita","year":"2017","journal-title":"Front. Plant Sci."},{"doi-asserted-by":"crossref","unstructured":"Heckathorn, S.A., Giri, A., Mishra, S., and Bista, D. (2013). Heat Stress and Roots. Climate Change and Plant Abiotic Stress Tolerance, Wiley.","key":"ref_41","DOI":"10.1002\/9783527675265.ch05"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"114478","DOI":"10.1016\/j.geoderma.2020.114478","article-title":"Effects of crack morphology on soil carbon flux dynamics in a dryland vertisol","volume":"375","author":"DeCarlo","year":"2020","journal-title":"Geoderma"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"59","DOI":"10.31545\/intagr\/156632","article-title":"Long-term contrasting tillage in Cambisol: Effect on water-stable aggregates, macropore network and soil chemical properties","volume":"37","author":"Kochiieru","year":"2023","journal-title":"Int. Agrophys."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"133586","DOI":"10.1016\/j.chemosphere.2022.133586","article-title":"Biochar considerably increases the easily available water and nutrient content in low-organic soils amended with compost and manure","volume":"293","author":"Seyedsadr","year":"2022","journal-title":"Chemosphere"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"131003","DOI":"10.1016\/j.foodchem.2021.131003","article-title":"Metabolomics profile responses to changing environments in a common bean (Phaseolus vulgaris L.) germplasm collection","volume":"370","author":"Mecha","year":"2022","journal-title":"Food Chem."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"100716","DOI":"10.1016\/j.xplc.2023.100716","article-title":"Challenges facing sustainable protein production: Opportunities for cereals","volume":"4","author":"Safdar","year":"2023","journal-title":"Plant Commun."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"989","DOI":"10.1038\/s41477-018-0309-4","article-title":"Developing naturally stress-resistant crops for a sustainable agriculture","volume":"4","author":"Zhang","year":"2018","journal-title":"Nat. Plants"}],"container-title":["Agriculture"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2077-0472\/15\/18\/1909\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:42:27Z","timestamp":1760035347000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2077-0472\/15\/18\/1909"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9,9]]},"references-count":47,"journal-issue":{"issue":"18","published-online":{"date-parts":[[2025,9]]}},"alternative-id":["agriculture15181909"],"URL":"https:\/\/doi.org\/10.3390\/agriculture15181909","relation":{},"ISSN":["2077-0472"],"issn-type":[{"type":"electronic","value":"2077-0472"}],"subject":[],"published":{"date-parts":[[2025,9,9]]}}}