{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T01:39:07Z","timestamp":1776735547568,"version":"3.51.2"},"reference-count":55,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2021,1,24]],"date-time":"2021-01-24T00:00:00Z","timestamp":1611446400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["PTDC\/AGR-TEC\/3555\/2012"],"award-info":[{"award-number":["PTDC\/AGR-TEC\/3555\/2012"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["SFRH\/BD\/89287\/2012"],"award-info":[{"award-number":["SFRH\/BD\/89287\/2012"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"name":"GREEN-IT - Bioresources for sustainability","award":["R&D Unit, UIDB\/04551\/2020"],"award-info":[{"award-number":["R&D Unit, UIDB\/04551\/2020"]}]},{"name":"PORTUGAL 2020","award":["LISBOA-01-0145-FEDER-402-022125"],"award-info":[{"award-number":["LISBOA-01-0145-FEDER-402-022125"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Agronomy"],"abstract":"<jats:p>The common bean (Phaseolus vulgaris L.) represents a sustainable and affordable source of protein, namely, to populations with vegetarian dietary habits. Despite the national germplasm genetic diversity, little is known about the Portuguese accessions\u2019 nutritional and protein quality, leading to their underuse in breeding programs. To fill this gap, a representative collection (106 accessions) was cropped under two contrasting environments (traditional versus heat stress) and evaluated in terms of nutritional quality by near-infrared spectroscopy. Protein quality was assessed, under the stressful environment, considering the individual amino acid contents and the activity of trypsin inhibitors through mass spectrometry (LC-MS\/MS) and spectrophotometry, respectively. On top of strong genotypic control, the nutritional composition (protein, fat, fiber, moisture and ash) was also highly influenced by the environment and by genotype \u00d7 environment interaction, with a clear nutritional quality ranking change for the accessions in heat stress conditions. Classified into three clusters, the accessions from the cluster with the highest individual amino acid and protein contents also showed higher trypsin inhibitor activity (TIA). Since different levels of TIA had no translation into contrasting protein digestibility, breeders focusing on common beans\u2019 protein quality improvement, especially under challenging warming climate conditions, may take advantage of this group of accessions.<\/jats:p>","DOI":"10.3390\/agronomy11020221","type":"journal-article","created":{"date-parts":[[2021,1,25]],"date-time":"2021-01-25T02:07:00Z","timestamp":1611540420000},"page":"221","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Disclosing the Nutritional Quality Diversity of Portuguese Common Beans\u2014The Missing Link for Their Effective Use in Protein Quality Breeding Programs"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2340-6244","authenticated-orcid":false,"given":"Elsa","family":"Mecha","sequence":"first","affiliation":[{"name":"ITQB NOVA, Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa, Av. da Rep\u00fablica, 2780-157 Oeiras, Portugal"}]},{"given":"Sofia","family":"Natalello","sequence":"additional","affiliation":[{"name":"ITQB NOVA, Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa, Av. da Rep\u00fablica, 2780-157 Oeiras, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5941-8749","authenticated-orcid":false,"given":"Bruna","family":"Carbas","sequence":"additional","affiliation":[{"name":"INIAV, Instituto Nacional de Investiga\u00e7\u00e3o Agr\u00e1ria e Veterin\u00e1ria, Av. da Rep\u00fablica, 2784-505 Oeiras, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3047-0586","authenticated-orcid":false,"given":"Andreia Bento","family":"da Silva","sequence":"additional","affiliation":[{"name":"ITQB NOVA, Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa, Av. da Rep\u00fablica, 2780-157 Oeiras, Portugal"},{"name":"FCT NOVA, Faculdade de Ci\u00eancias e Tecnologia da Universidade Nova de Lisboa, 2829-516 Caparica, Portugal"},{"name":"Faculdade de Farm\u00e1cia, Universidade de Lisboa, Av. das For\u00e7as Armadas, 1649-019 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6483-0455","authenticated-orcid":false,"given":"Susana T.","family":"Leit\u00e3o","sequence":"additional","affiliation":[{"name":"ITQB NOVA, Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa, Av. da Rep\u00fablica, 2780-157 Oeiras, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1949-172X","authenticated-orcid":false,"given":"Carla","family":"Brites","sequence":"additional","affiliation":[{"name":"INIAV, Instituto Nacional de Investiga\u00e7\u00e3o Agr\u00e1ria e Veterin\u00e1ria, Av. da Rep\u00fablica, 2784-505 Oeiras, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1995-095X","authenticated-orcid":false,"given":"Maria Manuela","family":"Veloso","sequence":"additional","affiliation":[{"name":"INIAV, Instituto Nacional de Investiga\u00e7\u00e3o Agr\u00e1ria e Veterin\u00e1ria, Av. da Rep\u00fablica, 2784-505 Oeiras, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9644-8616","authenticated-orcid":false,"given":"Diego","family":"Rubiales","sequence":"additional","affiliation":[{"name":"IAS, Institute for Sustainable Agriculture, CSIC, Avda Men\u00e9ndez Pidal s\/n, 14004 C\u00f3rdoba, Spain"}]},{"given":"Judite","family":"Costa","sequence":"additional","affiliation":[{"name":"Faculdade de Farm\u00e1cia, Universidade de Lisboa, Av. das For\u00e7as Armadas, 1649-019 Lisboa, Portugal"}]},{"given":"Maria de F\u00e1tima","family":"Cabral","sequence":"additional","affiliation":[{"name":"Faculdade de Farm\u00e1cia, Universidade de Lisboa, Av. das For\u00e7as Armadas, 1649-019 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1561-6858","authenticated-orcid":false,"given":"Maria E.","family":"Figueira","sequence":"additional","affiliation":[{"name":"Faculdade de Farm\u00e1cia, Universidade de Lisboa, Av. das For\u00e7as Armadas, 1649-019 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8469-7508","authenticated-orcid":false,"given":"Maria C.","family":"Vaz Patto","sequence":"additional","affiliation":[{"name":"ITQB NOVA, Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa, Av. da Rep\u00fablica, 2780-157 Oeiras, Portugal"}]},{"given":"Maria R.","family":"Bronze","sequence":"additional","affiliation":[{"name":"ITQB NOVA, Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa, Av. da Rep\u00fablica, 2780-157 Oeiras, Portugal"},{"name":"Faculdade de Farm\u00e1cia, Universidade de Lisboa, Av. das For\u00e7as Armadas, 1649-019 Lisboa, Portugal"},{"name":"iBET, Instituto de Biologia Experimental e Tecnol\u00f3gica, Av. da Rep\u00fablica, Apartado 12, 2781-901 Oeiras, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2021,1,24]]},"reference":[{"key":"ref_1","unstructured":"UNICEF, WHO, and World Bank Group (2020, September 29). 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