{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,19]],"date-time":"2026-04-19T06:08:47Z","timestamp":1776578927940,"version":"3.51.2"},"reference-count":95,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2022,1,8]],"date-time":"2022-01-08T00:00:00Z","timestamp":1641600000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Agronomy"],"abstract":"<jats:p>Drought is a major threat to coffee, compromising the quality and quantity of its production. We have analyzed the core proteome of 18 Coffea canephora cv. Conilon Clone 153 and C. arabica cv. Icatu plants and assessed their responses to moderate (MWD) and severe (SWD) water deficits. Label-free quantitative shotgun proteomics identified 3000 proteins in both genotypes, but less than 0.8% contributed to ca. 20% of proteome biomass. Proteomic changes were dependent on the severity of drought, being stronger under SWD and with an enrolment of different proteins, functions, and pathways than under MWD. The two genotypes displayed stress-responsive proteins under SWD, but only C. arabica showed a higher abundance of proteins involved in antioxidant detoxification activities. Overall, the impact of MWD was minor in the two genotypes, contrary to previous studies. In contrast, an extensive proteomic response was found under SWD, with C. arabica having a greater potential for acclimation\/resilience than C. canephora. This is likely supported by a wider antioxidative response and an ability to repair photosynthetic structures, being crucial to develop new elite genotypes that assure coffee supply under water scarcity levels.<\/jats:p>","DOI":"10.3390\/agronomy12010148","type":"journal-article","created":{"date-parts":[[2022,1,9]],"date-time":"2022-01-09T20:29:26Z","timestamp":1641760166000},"page":"148","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Next-Generation Proteomics Reveals a Greater Antioxidative Response to Drought in Coffea arabica Than in Coffea canephora"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9788-4831","authenticated-orcid":false,"given":"Isabel","family":"Marques","sequence":"first","affiliation":[{"name":"Plant Stress & Biodiversity Lab, Centro de Estudos Florestais (CEF), Instituto Superior Agronomia (ISA), Universidade de Lisboa (ULisboa), Tapada da Ajuda, 1349-017 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5578-3370","authenticated-orcid":false,"given":"Duarte","family":"Gouveia","sequence":"additional","affiliation":[{"name":"D\u00e9partement M\u00e9dicaments et Technologies pour la Sant\u00e9 (DMTS), Universit\u00e9 Paris-Saclay, CEA, INRAE, SPI, F-F-30200 Bagnols-sur-C\u00e8ze, France"}]},{"given":"Jean-Charles","family":"Gaillard","sequence":"additional","affiliation":[{"name":"D\u00e9partement M\u00e9dicaments et Technologies pour la Sant\u00e9 (DMTS), Universit\u00e9 Paris-Saclay, CEA, INRAE, SPI, F-F-30200 Bagnols-sur-C\u00e8ze, France"}]},{"given":"S\u00f3nia","family":"Martins","sequence":"additional","affiliation":[{"name":"Unidade de Geobioci\u00eancias, Geoengenharias e Geotecnologias (GeoBioTec), Faculdade de Ci\u00eancias e Tecnologia (FCT), Universidade NOVA de Lisboa (UNL), Monte de Caparica, 2829-516 Caparica, Portugal"},{"name":"Departamento de Engenharia Qu\u00edmica, Instituto Superior de Engenharia de Lisboa, Instituto Polit\u00e9cnico de Lisboa, R. Conselheiro Em\u00eddio Navarro 1, 1959-007 Lisboa, Portugal"}]},{"given":"Magda C.","family":"Semedo","sequence":"additional","affiliation":[{"name":"Unidade de Geobioci\u00eancias, Geoengenharias e Geotecnologias (GeoBioTec), Faculdade de Ci\u00eancias e Tecnologia (FCT), Universidade NOVA de Lisboa (UNL), Monte de Caparica, 2829-516 Caparica, Portugal"},{"name":"Departamento de Engenharia Qu\u00edmica, Instituto Superior de Engenharia de Lisboa, Instituto Polit\u00e9cnico de Lisboa, R. Conselheiro Em\u00eddio Navarro 1, 1959-007 Lisboa, Portugal"}]},{"given":"Fernando C.","family":"Lidon","sequence":"additional","affiliation":[{"name":"Unidade de Geobioci\u00eancias, Geoengenharias e Geotecnologias (GeoBioTec), Faculdade de Ci\u00eancias e Tecnologia (FCT), Universidade NOVA de Lisboa (UNL), Monte de Caparica, 2829-516 Caparica, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9637-8475","authenticated-orcid":false,"given":"F\u00e1bio M.","family":"DaMatta","sequence":"additional","affiliation":[{"name":"Departamento de Biologia Vegetal, Universidade Federal Vi\u00e7osa, Vi\u00e7osa 36570-900, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6071-6460","authenticated-orcid":false,"given":"Ana I.","family":"Ribeiro-Barros","sequence":"additional","affiliation":[{"name":"Plant Stress & Biodiversity Lab, Centro de Estudos Florestais (CEF), Instituto Superior Agronomia (ISA), Universidade de Lisboa (ULisboa), Tapada da Ajuda, 1349-017 Lisboa, Portugal"},{"name":"Unidade de Geobioci\u00eancias, Geoengenharias e Geotecnologias (GeoBioTec), Faculdade de Ci\u00eancias e Tecnologia (FCT), Universidade NOVA de Lisboa (UNL), Monte de Caparica, 2829-516 Caparica, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1589-445X","authenticated-orcid":false,"given":"Jean","family":"Armengaud","sequence":"additional","affiliation":[{"name":"D\u00e9partement M\u00e9dicaments et Technologies pour la Sant\u00e9 (DMTS), Universit\u00e9 Paris-Saclay, CEA, INRAE, SPI, F-F-30200 Bagnols-sur-C\u00e8ze, France"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7639-7214","authenticated-orcid":false,"given":"Jos\u00e9 C.","family":"Ramalho","sequence":"additional","affiliation":[{"name":"Plant Stress & Biodiversity Lab, Centro de Estudos Florestais (CEF), Instituto Superior Agronomia (ISA), Universidade de Lisboa (ULisboa), Tapada da Ajuda, 1349-017 Lisboa, Portugal"},{"name":"Unidade de Geobioci\u00eancias, Geoengenharias e Geotecnologias (GeoBioTec), Faculdade de Ci\u00eancias e Tecnologia (FCT), Universidade NOVA de Lisboa (UNL), Monte de Caparica, 2829-516 Caparica, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,1,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/B978-0-12-387689-8.00002-3","article-title":"Drought Tolerance. Roles of Organic Osmolytes, Growth Regulators, and Mineral Nutrients","volume":"111","author":"Ashraf","year":"2011","journal-title":"Adv. Agron."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1007\/s00344-013-9325-9","article-title":"Regulation in plant stress tolerance by a potential plant growth regulator, 5-aminolevulinic acid","volume":"32","author":"Akram","year":"2013","journal-title":"J. Plant Growth Regul."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1111\/1365-2745.12064","article-title":"Subordinate plant species enhance community resistance against drought in semi-natural grasslands","volume":"101","author":"Mariotte","year":"2013","journal-title":"J. Ecol."},{"key":"ref_4","unstructured":"Edenhofer, O., Pichs-Madruga, R., Sokona, Y., Farahani, E., Kadner, S., Seyboth, K., Adler, A., Baum, I., Brunner, S., and Eickemeier, P. (2014). Intergovernmental Panel on Climate Change. Climate Change 2014: Mitigation of Climate Change. Contribution of Working Group III to the Fifth Assessment Report of the Intergovernmental Panel on Climate Change, Cambridge University Press."},{"key":"ref_5","unstructured":"Masson-Delmotte, V., Zhai, P., P\u00f6rtner, H.O., Roberts, D., Skea, J., Shukla, P.R., Pirani, A., Moufouma-Okia, W., P\u00e9an, C., and Pidcock, R. (2018). Summary for Policymakers, World Meteorological Organization."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/S0098-8472(01)00130-7","article-title":"Photochemical responses and oxidative stress in two clones of Coffea canephora under water deficit conditions. Environ","volume":"47","author":"Lima","year":"2002","journal-title":"Exp. Bot."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1093\/aob\/mcn125","article-title":"Photosynthesis under drought and salt stress: Regulation mechanisms from whole plant to cell","volume":"103","author":"Chaves","year":"2009","journal-title":"Ann. Bot."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1051\/agro:2008021","article-title":"Plant drought stress: Effects, mechanisms and management","volume":"29","author":"Farooq","year":"2009","journal-title":"Agron. Sustain. Dev."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1007\/s40626-014-0001-7","article-title":"Cold impact and acclimation response of Coffea spp. plants","volume":"26","author":"Ramalho","year":"2014","journal-title":"Theor. Exp. Plant Physiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"4361","DOI":"10.1093\/jxb\/erq239","article-title":"The impact of drought on leaf physiology of Quercus suber L. trees: Comparison of an extreme drought event with chronic rainfall reduction","volume":"61","author":"Grant","year":"2010","journal-title":"J. Exp. Bot."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1023\/A:1024331414564","article-title":"Photosynthetic activity and cellular integrity of the Andean legume Pachyrhizus ahipa (Wedd.) Parodi under heat and water stress","volume":"40","author":"Matos","year":"2002","journal-title":"Photosynthetica"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1071\/FP02076","article-title":"Understanding plant responses to drought - from genes to the whole plant","volume":"30","author":"Chaves","year":"2003","journal-title":"Funct. Plant Biol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1715","DOI":"10.1093\/jxb\/erq438","article-title":"Water deficits uncouple growth from photosynthesis, increase C content, and modify the relationships between C and growth in sink organs","volume":"62","author":"Muller","year":"2011","journal-title":"J. Exp. Bot."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1147","DOI":"10.3389\/fpls.2017.01147","article-title":"Crop production under drought and heat stress: Plant responses and management options","volume":"8","author":"Fahad","year":"2017","journal-title":"Front. Plant Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"909","DOI":"10.1016\/j.plaphy.2010.08.016","article-title":"Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants","volume":"48","author":"Gill","year":"2010","journal-title":"Plant Physiol. Biochem."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1254","DOI":"10.1007\/s11356-015-5361-2","article-title":"Role of xylo-oligosaccharides in protection against salinity-induced adversities in Chinese cabbage","volume":"23","author":"Chen","year":"2016","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Hussain, S., Khan, F., Cao, W., Wu, L., and Geng, M. (2016). Seed priming alters the production and detoxification of reactive oxygen intermediates in rice seedlings grown under sub-optimal temperature and nutrient supply. Front. Plant Sci., 7.","DOI":"10.3389\/fpls.2016.00439"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1093\/pcp\/pcg053","article-title":"Cyclic electron flow within PSII protects PSII from its photoinhibition in thylakoid membranes from spinach chloroplasts","volume":"44","author":"Miyake","year":"2003","journal-title":"Plant Cell Physiol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1049","DOI":"10.3389\/fpls.2020.01049","article-title":"Resilient and sensitive key points of the photosynthetic machinery of Coffea spp. to the single and superimposed exposure to severe drought and heat stresses","volume":"11","author":"Dubberstein","year":"2020","journal-title":"Front. Plant Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"708","DOI":"10.1093\/treephys\/tpaa158","article-title":"Intrinsic non-stomatal resilience to drought of the photosynthetic apparatus in Coffea spp. is strengthened by elevated air [CO2]","volume":"41","author":"Semedo","year":"2021","journal-title":"Tree Physiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"2365","DOI":"10.1093\/jxb\/erh269","article-title":"Mechanisms underlying plant resilience to water deficits: Prospects for water-saving agriculture","volume":"55","author":"Chaves","year":"2004","journal-title":"J. Exp. Bot."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Hasan, M.M.U., Ma, F., Prodhan, Z.H., Li, F., Shen, H., Chen, Y., and Wang, X. (2018). Molecular and physio-biochemical characterization of cotton species for assessing drought stress tolerance. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19092636"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2996","DOI":"10.1111\/1462-2920.14975","article-title":"Quick microbial molecular phenotyping by differential shotgun proteomics","volume":"22","author":"Gouveia","year":"2020","journal-title":"Environ. Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"44","DOI":"10.3389\/fpls.2011.00044","article-title":"Quantitative proteomic analysis of wheat cultivars with differing drought stress tolerance","volume":"2","author":"Ford","year":"2011","journal-title":"Front. Plant Sci."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Hamzelou, S., Pascovici, D., Kamath, K.S., Amirkhani, A., McKay, M., Mirzaei, M., Atwell, B.J., and Haynes, P.A. (2020). Proteomic responses to drought vary widely among eight diverse genotypes of rice (Oryza sativa). Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21010363"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1021","DOI":"10.1007\/s10722-012-9898-3","article-title":"An assessment of the genetic integrity of ex situ germplasm collections of three endangered species of Coffea from Madagascar: Implications for the management of field germplasm collections","volume":"60","author":"Krishnan","year":"2013","journal-title":"Genet. Resour. Crop Evol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1590\/S1677-04202006000100006","article-title":"Impacts of drought and temperature stress on coffee physiology and production: A review","volume":"18","author":"DaMatta","year":"2006","journal-title":"Braz. J. Plant Physiol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"5264","DOI":"10.1021\/acs.jafc.7b04537","article-title":"Physiological and agronomic performance of the coffee crop in the context of climate change and global warming: A review","volume":"66","author":"DaMatta","year":"2018","journal-title":"J. Agric. Food Chem."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Marques, I., Fernandes, I., Paulo, O.S., Lidon, F.C., DaMatta, F.M., Ramalho, J.C., and Ribeiro-Barros, A.I. (2021). A transcriptomic approach to understanding the combined impacts of supra-optimal temperatures and CO2 revealed different responses in the polyploid Coffea arabica and its diploid progenitor C. canephora. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22063125"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/S0168-9452(02)00342-4","article-title":"Drought tolerance of two field-grown clones of Coffea canephora","volume":"164","author":"DaMatta","year":"2003","journal-title":"Plant Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1639","DOI":"10.1016\/j.jplph.2006.12.004","article-title":"Morphological and physiological responses of two coffee progenies to soil water availability","volume":"164","author":"Dias","year":"2007","journal-title":"J. Plant Physiol."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"De Oliveira Santos, M., Coelho, L.S., Carvalho, G.R., Botelho, C.E., Torres, L.F., Vilela, D.J.M., Andrade, A.C., and Silva, V.A. (2021). Photochemical efficiency correlated with candidate gene expression promote coffee drought tolerance. Sci. Rep., 11.","DOI":"10.1038\/s41598-021-86689-y"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Rodrigues, A.M., Jorge, T., Osorio, S., Pott, D.M., Lidon, F.C., DaMatta, F.M., Marques, I., Ribeiro-Barros, A.I., Ramalho, J.C., and Ant\u00f3nio, C. (2021). Primary metabolite profile changes in Coffea spp. promoted by single and combined exposure to drought and elevated [CO2]. Metabolites, 11.","DOI":"10.3390\/metabo11070427"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/j.jplph.2013.07.007","article-title":"Phospholipids profile in chloroplasts of Coffea spp. genotypes differing in cold acclimation ability","volume":"171","author":"Pais","year":"2014","journal-title":"J. Plant Physiol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"103856","DOI":"10.1016\/j.envexpbot.2019.103856","article-title":"Lipid profile adjustments may contribute to warming acclimation and to heat impact mitigation by elevated [CO2] in Coffea spp.","volume":"167","author":"Pais","year":"2019","journal-title":"Environ. Exp. Bot."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1016\/j.envexpbot.2011.06.001","article-title":"Characterization of the main lipid components of chloroplast membranes and cold induced changes in Coffea spp.","volume":"74","author":"Partelli","year":"2011","journal-title":"Environ. Exp. Bot."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1007\/s10584-018-2346-4","article-title":"Why could the coffee crop endure climate change and global warming to a greater extent than previously estimated?","volume":"152","author":"DaMatta","year":"2019","journal-title":"Clim. Chang."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"947","DOI":"10.3389\/fpls.2016.00947","article-title":"Protective response mechanisms to heat stress in interaction with high [CO2] conditions in Coffea spp.","volume":"7","author":"Martins","year":"2016","journal-title":"Front. Plant Sci."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1093\/treephys\/10.2.127","article-title":"Effects of water deficit on flower opening in coffee (Coffea arabica L.)","volume":"10","author":"Crisosto","year":"1992","journal-title":"Tree Physiol."},{"key":"ref_40","first-page":"65","article-title":"Definition and outline for the phenological phases of arabic coffee under Brazilian tropical conditions","volume":"60","year":"2001","journal-title":"Bragantia"},{"key":"ref_41","unstructured":"CONAB (2021, November 10). S\u00e9rie Hist\u00f3rica das Safras, Available online: https:\/\/portaldeinformacoes.conab.gov.br\/index.php\/safras\/cafe-serie-historic."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Semedo, J.N., Rodrigues, W.P., Dubberstein, D., Martins, M.Q., Martins, L.D., Pais, I.P., Rodrigues, A.P., Leit\u00e3o, A.E., Partelli, F.L., and Campostrini, E. (2018). Coffee responses to drought, warming and high [CO2] in a context of future climate change scenarios. Climate Change Management, Springer.","DOI":"10.1007\/978-3-319-72874-2_26"},{"key":"ref_43","unstructured":"Ferr\u00e3o, R.G., Fonseca, A.F.A., Bragan\u00e7a, S.M., Ferr\u00e3o, M.A.G., and Muner, L.H. (2007). Cultivares de caf\u00e9 Conilon, Caf\u00e9 Conilon, Chapter 7."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Fernandes, I., Marques, I., Paulo, O.S., Batista, D., Partelli, F.L., Lidon, F.C., DaMatta, F.M., Ramalho, J.C., and Ribeiro-Barros, A.I. (2021). Understanding the Impact of Drought in Coffea Genotypes: Transcriptomic Analysis Supports a Common High Resilience to Moderate Water Deficit but a Genotype Dependent Sensitivity to Severe Water Deficit. Agronomy, 11.","DOI":"10.3390\/agronomy11112255"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Ramalho, J.C., Rodrigues, A.P., Semedo, J.N., Pais, I.P., Martins, L.D., Sim\u00f5es-Costa, M.C., Leit\u00e3o, A.E., Fortunato, A.S., Batista-Santos, P., and Palos, I.M. (2013). Sustained photosynthetic performance of Coffea spp. under long-term enhanced [CO2]. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0082712"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Ramalho, J.C., Rodrigues, A.P., Lidon, F.C., Marques, L.M.C., Leit\u00e3o, A.E., Fortunato, A.S., Pais, I.P., Silva, M.J., Scotti-Campos, P., and Lopes, A. (2018). Stress cross-response of the antioxidative system promoted by superimposed drought and cold conditions in Coffea spp.. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0198694"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"3622","DOI":"10.1002\/jssc.201500415","article-title":"Efficient extraction of proteins from recalcitrant plant tissue for subsequent analysis by two-dimensional gel electrophoresis","volume":"38","author":"Parkhey","year":"2015","journal-title":"J. Sep. Sci."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal. Biochem."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1007\/978-1-4939-1261-2_16","article-title":"Taking the shortcut for high-throughput shotgun proteomic analysis of bacteria","volume":"1197","author":"Hartmann","year":"2014","journal-title":"Methods Mol. Biol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1555","DOI":"10.1080\/17435390.2016.1244299","article-title":"RNA-binding proteins are a major target of silica nanoparticles in cell extracts","volume":"10","author":"Klein","year":"2016","journal-title":"Nanotoxicology"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1181","DOI":"10.1126\/science.1255274","article-title":"The coffee genome provides insight into the convergent evolution of caffeine biosynthesis","volume":"345","author":"Denoeud","year":"2014","journal-title":"Science"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1038\/ismej.2011.86","article-title":"Proteomic insights into the lifestyle of an environmentally relevant marine bacterium","volume":"6","author":"Fernandez","year":"2012","journal-title":"ISME J."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Hulsen, T., de Vlieg, J., and Alkema, W. (2008). BioVenn\u2014A web application for the comparison and visualization of biological lists using area-proportional Venn diagrams. BMC Genom., 9.","DOI":"10.1186\/1471-2164-9-488"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1186\/s13059-019-1758-4","article-title":"Cytoscape Automation: Empowering workflow-based network analysis","volume":"20","author":"Otasek","year":"2019","journal-title":"Genome Biol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1016\/j.jprot.2014.01.007","article-title":"Non-model organisms, a species endangered by proteogenomics","volume":"105","author":"Armengaud","year":"2014","journal-title":"J. Proteom."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Cao, Y., Luo, Q., Tian, Y., and Meng, F. (2017). Physiological and proteomic analyses of the drought stress response in Amygdalus mira (Koehne) Y\u00fc et Lu roots. BMC Plant Biol., 17.","DOI":"10.1186\/s12870-017-1000-z"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1007\/s10725-020-00643-y","article-title":"Comparative proteomic analysis of drought and high temperature response in roots of two potato cultivars","volume":"92","author":"Gietler","year":"2020","journal-title":"Plant Growth Regul."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1186\/1477-5956-9-7","article-title":"In-depth analysis of the chicken egg white proteome using an LTQ Orbitrap Velos","volume":"9","author":"Mann","year":"2011","journal-title":"Proteome Sci."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1002\/mas.21365","article-title":"A decade of plant proteomics and mass spectrometry: Translation of technical advancements to food security and safety issues","volume":"32","author":"Agrawal","year":"2013","journal-title":"Mass Spectrom. Rev."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"1273","DOI":"10.1101\/gr.213694.116","article-title":"The developmental proteome of Drosophila melanogaster","volume":"27","author":"Bluhm","year":"2017","journal-title":"Genome Res."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1038\/s41598-020-61216-7","article-title":"A single polyploidization event at the origin of the tetraploid genome of Coffea arabica is responsible for the extremely low genetic variation in wild and cultivated germplasm","volume":"10","author":"Scalabrin","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1111\/gcb.13088","article-title":"Long-term elevated air [CO2] strengthens photosynthetic functioning and mitigates the impact of supra-optimal temperatures in tropical Coffea arabica and C. canephora species","volume":"22","author":"Rodrigues","year":"2016","journal-title":"Glob. Chang. Biol."},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Marques, I., Fernandes, I., David, P.H.C., Paulo, O.S., Goulao, L.F., Fortunato, A.S., Lidon, F.C., DaMatta, F.M., Ramalho, J.C., and Ribeiro-Barros, A.I. (2020). Transcriptomic leaf profiling reveals differential responses of the two most traded coffee species to elevated [CO2]. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21239211"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"4021","DOI":"10.1038\/s41467-019-12002-1","article-title":"Auxin-sensitive Aux\/IAA proteins mediate drought tolerance in Arabidopsis by regulating glucosinolate levels","volume":"10","author":"Salehin","year":"2019","journal-title":"Nat. Commun."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1104\/pp.126.1.342","article-title":"Phytochrome-mediated photoperiod perception, shoot growth, glutamine, calcium, and protein phosphorylation influence the activity of the poplar bark storage protein gene promoter (bspA)","volume":"126","author":"Zhu","year":"2001","journal-title":"Plant Physiol."},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Pettengill, E.A., Pettengill, J.B., and Coleman, G.D. (2013). Elucidating the evolutionary history and expression patterns of nucleoside phosphorylase paralogs (vegetative storage proteins) in Populus and the plant kingdom. BMC Plant Biol., 13.","DOI":"10.1186\/1471-2229-13-118"},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Skorupa, M., Go\u0142\u0229biewski, M., Kurnik, K., Niedojad\u0142o, J., K\u0229sy, J., Klamkowski, K., W\u00f3jcik, K., Treder, W., Tretyn, A., and Tyburski, J. (2019). Salt stress vs. salt shock\u2014The case of sugar beet and its halophytic ancestor. BMC Plant Biol., 19.","DOI":"10.1186\/s12870-019-1661-x"},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"Sultana, N., Islam, S., Juhasz, A., Yang, R., She, M., Alhabbar, Z., Zhang, J., and Ma, W. (2020). Transcriptomic study for identification of major nitrogen stress responsive genes in australian bread wheat cultivars. Front. Genet., 11.","DOI":"10.3389\/fgene.2020.583785"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1071\/FP15057","article-title":"Heterogeneity of photosynthesis within leaves is associated with alteration of leaf structural features and leaf N content per leaf area in rice","volume":"42","author":"Xiong","year":"2015","journal-title":"Funct. Plant Biol."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1016\/j.pbi.2009.04.015","article-title":"Root uptake regulation: A central process for NPS homeostasis in plants","volume":"12","author":"Gojon","year":"2009","journal-title":"Curr. Opin. Plant Biol."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1111\/nph.13892","article-title":"Alleviation of proton toxicity by nitrate uptake specifically depends on nitrate transporter 1.1 in Arabidopsis","volume":"211","author":"Fang","year":"2016","journal-title":"New Phytol."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"552","DOI":"10.5423\/PPJ.OA.06.2016.0141","article-title":"Overexpression of a pathogenesis-related protein 10 enhances biotic and abiotic stress tolerance in rice","volume":"32","author":"Wu","year":"2016","journal-title":"Plant Pathol. J."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"4089","DOI":"10.1016\/j.ygeno.2020.07.004","article-title":"Pathogenesis related protein-1 (PR-1) genes in tomato (Solanum lycopersicum L.): Bioinformatics analyses and expression profiles in response to drought stress","volume":"112","author":"Akbudak","year":"2020","journal-title":"Genomics"},{"key":"ref_74","doi-asserted-by":"crossref","unstructured":"Wang, J., Mao, X., Wang, R., Li, A., Zhao, G., Zhao, J., and Jing, R. (2019). Identification of wheat stress-responding genes and TaPR-1-1 function by screening a cDNA yeast library prepared following abiotic stress. Sci. Rep., 9.","DOI":"10.1038\/s41598-018-37859-y"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1080\/17429145.2015.1068386","article-title":"Physiological and biochemical traits of drought tolerance in Argania spinosa","volume":"10","author":"Chakhchar","year":"2015","journal-title":"J. Plant Interact."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1016\/S0022-2836(02)00115-8","article-title":"Folding in vitro of light-harvesting chlorophyll a\/b protein is coupled with pigment binding","volume":"318","author":"Horn","year":"2002","journal-title":"J. Mol. Biol."},{"key":"ref_77","doi-asserted-by":"crossref","unstructured":"Jia, Y., Wong, D.C.J., Sweetman, C., Bruning, J.B., and Ford, C.M. (2015). New insights into the evolutionary history of plant sorbitol dehydrogenase. BMC Plant Biol., 15.","DOI":"10.1186\/s12870-015-0478-5"},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"1214","DOI":"10.1126\/science.7112124","article-title":"Living with water stress: Evolution of osmolyte systems","volume":"217","author":"Yancey","year":"1982","journal-title":"Science"},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"889","DOI":"10.1093\/jxb\/eru446","article-title":"Polyols in grape berry: Transport and metabolic adjustments as a physiological strategy for water-deficit stress tolerance in grapevine","volume":"66","author":"Conde","year":"2015","journal-title":"J. Exp. Bot."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"1581","DOI":"10.1093\/jxb\/ern053","article-title":"Rubisco, Rubisco activase, and global climate change","volume":"59","author":"Sage","year":"2008","journal-title":"J. Exp. Bot."},{"key":"ref_81","doi-asserted-by":"crossref","unstructured":"Perdomo, J.A., Cap\u00f3-Bau\u00e7\u00e0, S., Carmo-Silva, E., and Galm\u00e9s, J. (2017). Rubisco and rubisco activase play an important role in the biochemical limitations of photosynthesis in rice, wheat, and maize under high temperature and water deficit. Front. Plant Sci., 8.","DOI":"10.3389\/fpls.2017.00490"},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1093\/pcp\/pcp014","article-title":"Constitutive expression of a trypsin protease inhibitor confers multiple stress tolerance in transgenic tobacco","volume":"50","author":"Srinivasan","year":"2009","journal-title":"Plant Cell Physiol."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"21435","DOI":"10.1038\/srep21435","article-title":"Different pollinator assemblages ensure reproductive success of Cleisostoma linearilobatum (Orchidaceae) in fragmented holy hill forest and traditional tea garden","volume":"6","author":"Zhou","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"490","DOI":"10.1016\/j.tplants.2004.08.009","article-title":"Reactive oxygen gene network of plants","volume":"9","author":"Mittler","year":"2004","journal-title":"Trends Plant Sci."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/S0168-9452(98)00073-9","article-title":"Nitrogen dependent changes in antioxidant system and in fatty acid composition of chloroplast membranes from Coffea arabica L. plants submitted to high irradiance","volume":"135","author":"Ramalho","year":"1998","journal-title":"Plant Sci."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1016\/j.jplph.2009.10.013","article-title":"Biochemical and molecular characterization of the antioxidative system of Coffea sp. under cold conditions in genotypes with contrasting tolerance","volume":"167","author":"Fortunato","year":"2010","journal-title":"J. Plant Physiol."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1016\/S1369-5266(02)00255-8","article-title":"Enhancement of tolerance of abiotic stress by metabolic engineering of betaines and other compatible solutes","volume":"5","author":"Chen","year":"2002","journal-title":"Curr. Opin. Plant Biol."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"476","DOI":"10.17221\/3469-PSE","article-title":"Ion accumulation in different organs of green bean genotypes grown under salt stress","volume":"52","author":"Yasar","year":"2006","journal-title":"Plant Soil Environ."},{"key":"ref_89","doi-asserted-by":"crossref","unstructured":"Wang, H., Zhang, M., Guo, R., Shi, D., Liu, B., Lin, X., and Yang, C. (2012). Effects of salt stress on ion balance and nitrogen metabolism of old and young leaves in rice (Oryza sativa L.). BMC Plant Biol., 12.","DOI":"10.1186\/1471-2229-12-194"},{"key":"ref_90","first-page":"353","article-title":"The low energy signaling network","volume":"5","author":"Adamo","year":"2014","journal-title":"Front. Plant Sci."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"2035","DOI":"10.1016\/S1875-2780(09)60087-0","article-title":"Constructing SSH library of cotton under drought stress and analysis of drought associated genes","volume":"36","author":"Wang","year":"2010","journal-title":"Acta Agron. Sin."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1111\/j.1438-8677.2012.00710.x","article-title":"Emerging concept for the role of photorespiration as an important part of abiotic stress response","volume":"15","author":"Voss","year":"2013","journal-title":"Plant Biol."},{"key":"ref_93","doi-asserted-by":"crossref","unstructured":"Lou, L., Li, X., Chen, J., Li, Y., Tang, Y., and Lv, J. (2018). Photosynthetic and ascorbate-glutathione metabolism in the flag leaves as compared to spikes under drought stress of winter wheat (Triticum aestivum L.). PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0194625"},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"156","DOI":"10.4161\/psb.3.3.5536","article-title":"Drought stress and reactive oxygen species: Production, scavenging and signaling","volume":"3","year":"2008","journal-title":"Plant Signal. Behav."},{"key":"ref_95","doi-asserted-by":"crossref","unstructured":"Ahmad, N., Malagoli, M., Wirtz, M., and Hell, R. (2016). Drought stress in maize causes differential acclimation responses of glutathione and sulfur metabolism in leaves and roots. BMC Plant Biol., 16.","DOI":"10.1186\/s12870-016-0940-z"}],"container-title":["Agronomy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4395\/12\/1\/148\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T14:01:43Z","timestamp":1760364103000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4395\/12\/1\/148"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,1,8]]},"references-count":95,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2022,1]]}},"alternative-id":["agronomy12010148"],"URL":"https:\/\/doi.org\/10.3390\/agronomy12010148","relation":{},"ISSN":["2073-4395"],"issn-type":[{"value":"2073-4395","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,1,8]]}}}