{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T06:18:54Z","timestamp":1775197134301,"version":"3.50.1"},"reference-count":49,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2022,2,26]],"date-time":"2022-02-26T00:00:00Z","timestamp":1645833600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Agronomy"],"abstract":"<jats:p>The overapplication of manure on agricultural soils leads to nitrogen and phosphorus discharge into the aquatic environment, resulting in serious eutrophication problems and decreased water quality. Piggery wastewater (PWW) can be treated by microalgae to recycle nutrients, but the toxic levels of ammonia and organic matter hinder their growth. Fresh water is usually used to dilute PWW, but it is a scarce resource. The implementation of a pretreatment step before microalgae-based treatment could make PWW suitable for microalgae growth. Electrocoagulation, ammonia stripping, photo-Fenton, and constructed wetlands were evaluated as pretreatment methods to reduce ammonia, chemical oxygen demand (COD), color, and total suspended solids. Moreover, the pretreated PWWs were tested to grow the microalga Tetradesmus obliquus. Photo-Fenton showed the best results among the other pretreatments, achieving removal efficiencies above 90%, except for ammonia. This resulted in T. obliquus being capable of growing on undiluted PWW, even at higher ammonia levels, achieving similar biomass productivity to synthetic medium (66.4 \u00b1 17.8 mg\u00b7L\u22121\u00b7day\u22121 and 60.1 \u00b1 10.4 mg\u00b7L\u22121\u00b7day\u22121, respectively) almost doubling with pH control (116.5 mg\u00b7L\u22121\u00b7day\u22121). Thus, this pretreatment seems to be the most promising one to incorporate into microalgae-based treatment systems and must be further explored.<\/jats:p>","DOI":"10.3390\/agronomy12030580","type":"journal-article","created":{"date-parts":[[2022,2,27]],"date-time":"2022-02-27T20:46:17Z","timestamp":1645994777000},"page":"580","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Exploring Different Pretreatment Methodologies for Allowing Microalgae Growth in Undiluted Piggery Wastewater"],"prefix":"10.3390","volume":"12","author":[{"given":"Alice","family":"Ferreira","sequence":"first","affiliation":[{"name":"LNEG, National Laboratory of Energy and Geology I.P., Bioenergy Unit, Estrada do Pa\u00e7o do Lumiar 22, 1649-038 Lisbon, Portugal"}]},{"given":"Daniel","family":"Figueiredo","sequence":"additional","affiliation":[{"name":"GreenCoLab\u2014Associa\u00e7\u00e3o Oceano Verde, University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal"}]},{"given":"Rodrigo","family":"Cardeiras","sequence":"additional","affiliation":[{"name":"LNEG, National Laboratory of Energy and Geology I.P., Bioenergy Unit, Estrada do Pa\u00e7o do Lumiar 22, 1649-038 Lisbon, Portugal"}]},{"given":"Rui","family":"Nabais","sequence":"additional","affiliation":[{"name":"LNEG, National Laboratory of Energy and Geology I.P., Bioenergy Unit, Estrada do Pa\u00e7o do Lumiar 22, 1649-038 Lisbon, Portugal"}]},{"given":"Francisca","family":"Ferreira","sequence":"additional","affiliation":[{"name":"LNEG, National Laboratory of Energy and Geology I.P., Bioenergy Unit, Estrada do Pa\u00e7o do Lumiar 22, 1649-038 Lisbon, Portugal"}]},{"given":"Belina","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"LNEG, National Laboratory of Energy and Geology I.P., Bioenergy Unit, Estrada do Pa\u00e7o do Lumiar 22, 1649-038 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8864-2611","authenticated-orcid":false,"given":"Cl\u00e1udia M. d. S.","family":"Cordovil","sequence":"additional","affiliation":[{"name":"Forest Research Centre, School of Agriculture, University of Lisbon, Tapada da Ajuda, 1349-017 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8434-0365","authenticated-orcid":false,"given":"F. Gabriel","family":"Aci\u00e9n","sequence":"additional","affiliation":[{"name":"Chemical Engineering Department, University of Almeria, Ctra. Sacramento, s\/n, 04120 Almer\u00eda, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0196-6360","authenticated-orcid":false,"given":"Luisa","family":"Gouveia","sequence":"additional","affiliation":[{"name":"LNEG, National Laboratory of Energy and Geology I.P., Bioenergy Unit, Estrada do Pa\u00e7o do Lumiar 22, 1649-038 Lisbon, Portugal"},{"name":"GreenCoLab\u2014Associa\u00e7\u00e3o Oceano Verde, University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,2,26]]},"reference":[{"key":"ref_1","unstructured":"(2020, September 02). Eurostat Number of Pigs 2009\u20132019. 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