{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T20:26:51Z","timestamp":1777580811708,"version":"3.51.4"},"reference-count":45,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2022,4,21]],"date-time":"2022-04-21T00:00:00Z","timestamp":1650499200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["PTDC\/AGR-PRO\/2183\/2014"],"award-info":[{"award-number":["PTDC\/AGR-PRO\/2183\/2014"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["SFRH\/BD\/131766\/2017"],"award-info":[{"award-number":["SFRH\/BD\/131766\/2017"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["UIDB\/50017\/2020+UIDP\/50017\/2020"],"award-info":[{"award-number":["UIDB\/50017\/2020+UIDP\/50017\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["UIDB\/04129\/2020 and UIDP\/04129\/2020"],"award-info":[{"award-number":["UIDB\/04129\/2020 and UIDP\/04129\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"name":"COMPETE program","award":["PO-CI-01-0145-FEDER-016788"],"award-info":[{"award-number":["PO-CI-01-0145-FEDER-016788"]}]},{"name":"Programa Operacional Regional de Lisboa-POR Lisboa","award":["LISBOA-01-0145-FEDER-016788"],"award-info":[{"award-number":["LISBOA-01-0145-FEDER-016788"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Agronomy"],"abstract":"<jats:p>Grapevine trunk diseases constitute one of the major problems for viticulture worldwide, with Botryosphaeria dieback considered as one of the most important of these diseases. In this work, we aimed to (i) evaluate the effect of the combination of two products, Esquive\u00ae (a Trichoderma-based product) and LC2017 (a low-copper-based product), in the control of Lasiodiplodia theobromae, by evaluating the internal lesion length caused by inoculation of this pathogen on greenhouse kept grapevines of cvs Cabernet Sauvignon and Touriga Nacional and, (ii) investigating their elicitor effect on plant defense responses, through the analysis of the expression of a set of genes. The pathogen was always re-isolated from the infected tissues and was able to cause wood discoloration. Touriga Nacional exhibited longer lesions than Cabernet Sauvignon, and the application of both products did not appear to reduce lesion length when compared to LC2017 applied alone. The elicitor effect of LC2017 on plant defense was confirmed by gene expression analysis, and no significant differences were found between plants treated with LC2017 and with both products. Moreover, a specific response related to the cultivar was verified, but this apparently unique interaction between product, cultivar and pathogen remains to be further investigated.<\/jats:p>","DOI":"10.3390\/agronomy12050996","type":"journal-article","created":{"date-parts":[[2022,4,22]],"date-time":"2022-04-22T05:07:22Z","timestamp":1650604042000},"page":"996","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Effect of the Combined Treatments with LC2017 and Trichoderma\u00a0atroviride Strain I-1237 on Disease Development and Defense Responses in Vines Infected by Lasiodiplodia theobromae"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6344-7916","authenticated-orcid":false,"given":"Pedro","family":"Reis","sequence":"first","affiliation":[{"name":"LEAF\u2014Linking Landscape, Environment, Agriculture and Food-Research Center, Associated Laboratory TERRA, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4005-0494","authenticated-orcid":false,"given":"Vincenzo","family":"Mondello","sequence":"additional","affiliation":[{"name":"Unit\u00e9 R\u00e9sistance Induite et Bioprotection des Plantes, URCA\u2014Universit\u00e9 de Reims Champagne-Ardenne, EA 4707 USC INRAE 1488, SFR Condorcet FR CNRS 3417, 51687 Reims, France"}]},{"given":"In\u00eas","family":"Diniz","sequence":"additional","affiliation":[{"name":"LEAF\u2014Linking Landscape, Environment, Agriculture and Food-Research Center, Associated Laboratory TERRA, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017 Lisbon, Portugal"},{"name":"CIFC\u2014Centro de Investiga\u00e7\u00e3o das Ferrugens do Cafeeiro, Instituto Superior de Agronomia, Universidade de Lisboa, 2784-505 Oeiras, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0117-2958","authenticated-orcid":false,"given":"Artur","family":"Alves","sequence":"additional","affiliation":[{"name":"CESAM\u2014Centre for Environmental and Marine Studies, Department of Biology, University of Aveiro, 3810-193 Aveiro, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8952-537X","authenticated-orcid":false,"given":"Cec\u00edlia","family":"Rego","sequence":"additional","affiliation":[{"name":"LEAF\u2014Linking Landscape, Environment, Agriculture and Food-Research Center, Associated Laboratory TERRA, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017 Lisbon, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2918-8812","authenticated-orcid":false,"given":"Florence","family":"Fontaine","sequence":"additional","affiliation":[{"name":"Unit\u00e9 R\u00e9sistance Induite et Bioprotection des Plantes, URCA\u2014Universit\u00e9 de Reims Champagne-Ardenne, EA 4707 USC INRAE 1488, SFR Condorcet FR CNRS 3417, 51687 Reims, France"}]}],"member":"1968","published-online":{"date-parts":[[2022,4,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1111\/j.1365-3059.2012.02674.x","article-title":"Grapevine trunk diseases: Complex and still poorly understood","volume":"62","author":"Bertsch","year":"2013","journal-title":"Plant Pathol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1094\/PDIS-04-17-0512-FE","article-title":"Managing grapevine trunk diseases with respect to etiology and epidemiology: Current strategies and future prospects","volume":"102","author":"Gramaje","year":"2018","journal-title":"Plant Dis."},{"key":"ref_3","unstructured":"De la Fuente, M., Fontaine, F., Gramaje, D., Armengol, J., Smart, R., Nagy, Z.A., Rego, C., and Corio-Costet, M. 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