{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T16:44:46Z","timestamp":1775580286727,"version":"3.50.1"},"reference-count":37,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2022,5,17]],"date-time":"2022-05-17T00:00:00Z","timestamp":1652745600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Brazilian Federal Agency for Support and Evaluation of Graduate Education","award":["Exterior-88881.170665\/2018-01"],"award-info":[{"award-number":["Exterior-88881.170665\/2018-01"]}]},{"name":"Brazilian Federal Agency for Support and Evaluation of Graduate Education","award":["APQ-02926-16"],"award-info":[{"award-number":["APQ-02926-16"]}]},{"name":"National Council for Scientific and Technological Development (CNPq)","award":["Exterior-88881.170665\/2018-01"],"award-info":[{"award-number":["Exterior-88881.170665\/2018-01"]}]},{"name":"National Council for Scientific and Technological Development (CNPq)","award":["APQ-02926-16"],"award-info":[{"award-number":["APQ-02926-16"]}]},{"name":"Funda\u00e7\u00e3o de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG)","award":["Exterior-88881.170665\/2018-01"],"award-info":[{"award-number":["Exterior-88881.170665\/2018-01"]}]},{"name":"Funda\u00e7\u00e3o de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG)","award":["APQ-02926-16"],"award-info":[{"award-number":["APQ-02926-16"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Agronomy"],"abstract":"<jats:p>Ectomycorrhizae are classified as biotechnology to increase the sustainability of planted forests, and fieldwork is needed to confirm its effectiveness. The growth of rooted cuttings of Eucalyptus clones GG100 and GG680, which had been previously inoculated in the nursery with ectomycorrhizal fungi, was evaluated after planting them in the field. The ectomycorrhizal fungi (EMF) inoculated were: Pisolithus microcarpus, Hysterangium gardneri, or Scleroderma areolatum. Uninoculated rooted cuttings were used as controls. The inoculated treatments and the uninoculated controls (low P control) were grown in a nursery with reduced phosphate fertilization. Additionally, uninoculated controls were grown on a substrate with complete phosphate nursery fertilization (high P control). After two months, the plant height of clone GG100 inoculated with P. microcarpus was 16% taller and of clone GG680 13% higher than the low P control treatment. At the same time, the collar diameter of the plants inoculated with H. gardneri and P. microcarpus was the same as in the high P control. At 12 months, the growth of the inoculated and low P control plants was the same as in the high P control. For ectomycorrhizal colonization, after six months, the mean percentage of colonized root tips was highest in plants inoculated with S. areolatum, followed by those inoculated with P. microcarpus and of the low P control. After one year, ECM colonization was equal in all treatments and 4.3 times greater than it was at 6 months. Inoculation with ECM fungi in the nursery boosts early plant growth after transfer to the field, although the effect depends on the specific ectomycorrhizal fungus and the clone. Further ectomycorrhizal colonization of Eucalyptus occurs naturally and increases with tree development in the field.<\/jats:p>","DOI":"10.3390\/agronomy12051204","type":"journal-article","created":{"date-parts":[[2022,5,17]],"date-time":"2022-05-17T08:34:29Z","timestamp":1652776469000},"page":"1204","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Eucalyptus Field Growth and Colonization of Clones Pre-Inoculated with Ectomycorrhizal Fungi"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1663-9514","authenticated-orcid":false,"given":"Lidiomar Soares","family":"da Costa","sequence":"first","affiliation":[{"name":"Institute of Agricultural Sciences, Federal University of Uberl\u00e2ndia, Monte Carmelo 38500-000, Minas Gerais, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9252-3065","authenticated-orcid":false,"given":"Paulo Henrique","family":"Grazziotti","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Plant Production, Forest Engineering Department, Faculty of Agricultural Sciences, Federal University of the Jequitinhonha and Mucuri Valleys, Campus JK, Diamantina 39100-000, Minas Gerais, Brazil"}]},{"given":"Arley Jos\u00e9","family":"Fonseca","sequence":"additional","affiliation":[{"name":"Department of Agriculture, Federal University of Lavras, Lavras 37200-900, Minas Gerais, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6549-1854","authenticated-orcid":false,"given":"D\u00e9bora C\u00edntia","family":"dos Santos Avelar","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Plant Production, Department of Agronomy, Faculty of Agricultural Sciences, Federal University of the Jequitinhonha and Mucuri Valleys, Campus JK, Diamantina 39100-000, Minas Gerais, Brazil"}]},{"given":"M\u00e1rcio Jos\u00e9","family":"Rossi","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology and Parasitology, Federal University of Santa Catarina, Florian\u00f3polis 88040-900, Santa Catarina, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2088-9965","authenticated-orcid":false,"given":"Enilson","family":"de Barros Silva","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Plant Production, Department of Agronomy, Faculty of Agricultural Sciences, Federal University of the Jequitinhonha and Mucuri Valleys, Campus JK, Diamantina 39100-000, Minas Gerais, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9807-9834","authenticated-orcid":false,"given":"Eliane Cristine Soares","family":"da Costa","sequence":"additional","affiliation":[{"name":"Faculty of Agricultural and Veterinary Sciences, S\u00e3o Paulo State University, Jaboticabal 14883-125, S\u00e3o Paulo, Brazil"}]},{"given":"Danielle Cristina Fonseca Santos","family":"Grazziotti","sequence":"additional","affiliation":[{"name":"Instituto de Desenvolvimento do Norte e Nordeste de Minas Gerais, Diamantina 39100-000, Minas Gerais, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1822-5473","authenticated-orcid":false,"given":"Carla","family":"Ragonezi","sequence":"additional","affiliation":[{"name":"ISOPlexis Center, Campus da Penteada, University of Madeira, 9020-105 Funchal, Portugal"},{"name":"Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Tr\u00e1s-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,5,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1080\/10549811.2015.1102072","article-title":"Effect of shade on biomass accumulation and partitioning for Eucalyptus camaldulensis sprouts","volume":"35","author":"Righi","year":"2016","journal-title":"J. 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