{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T02:31:55Z","timestamp":1776393115880,"version":"3.51.2"},"reference-count":48,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2022,5,20]],"date-time":"2022-05-20T00:00:00Z","timestamp":1653004800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"CERNAS Research Centre (Polytechnic Institute of Viseu, Portugal)","award":["PROJ\/IPV\/ID&I\/021"],"award-info":[{"award-number":["PROJ\/IPV\/ID&I\/021"]}]},{"name":"CERNAS Research Centre (Polytechnic Institute of Viseu, Portugal)","award":["UIDB\/00681\/2020"],"award-info":[{"award-number":["UIDB\/00681\/2020"]}]},{"name":"CERNAS Research Centre (Polytechnic Institute of Viseu, Portugal)","award":["UIDB\/05507\/2020"],"award-info":[{"award-number":["UIDB\/05507\/2020"]}]},{"name":"CERNAS Research Centre (Polytechnic Institute of Viseu, Portugal)","award":["UIDB\/00742\/2020"],"award-info":[{"award-number":["UIDB\/00742\/2020"]}]},{"name":"FCT\u2014Foundation for Science and Technology (Portugal)","award":["PROJ\/IPV\/ID&I\/021"],"award-info":[{"award-number":["PROJ\/IPV\/ID&I\/021"]}]},{"name":"FCT\u2014Foundation for Science and Technology (Portugal)","award":["UIDB\/00681\/2020"],"award-info":[{"award-number":["UIDB\/00681\/2020"]}]},{"name":"FCT\u2014Foundation for Science and Technology (Portugal)","award":["UIDB\/05507\/2020"],"award-info":[{"award-number":["UIDB\/05507\/2020"]}]},{"name":"FCT\u2014Foundation for Science and Technology (Portugal)","award":["UIDB\/00742\/2020"],"award-info":[{"award-number":["UIDB\/00742\/2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Agronomy"],"abstract":"<jats:p>Agri-food waste has proved to be a valuable bioresource that can be used to obtain a variety of valuable materials, ingredients and chemicals. The optimum conditions for extracting bioactive compounds from sweet cherry seeds (SCS) with different solvents and temperatures were tested in this work. The choice criteria were based on the most efficient extracting capacity while looking for cleaner techniques with lower health or environmental impacts. Some extracting solvents (methanol, ethanol and water) were tested in different combinations and temperatures. The obtained extracts were evaluated for total phenolic compounds and some families of phenolics as well, using spectrophotometric methods. The results obtained showed that the highest extraction of total phenolic compounds was at 70 \u00b0C with 60:40 ratio water:ethanol (2.65 mg GAE\/g), while maximum flavonoids were obtained at 80 \u00b0C and 50% ethanolic aqueous solution (7.26 mg QE\/g). The highest value for ortho-diphenols was 21.47 mg GAE\/g for 50 \u00b0C and water:ethanol 50:50 solution. The highest proanthocyanidins and flavonols were obtained for 50:50 solution at 70 \u00b0C (6.43 mg CE\/g and 3.88 mg QE\/g, respectively), while the same solution at 80 \u00b0C allowed obtaining maximum phenolic acids (1.68 mg CAE\/g). The extraction of anthocyanins was found to vary significantly with concentration and temperature, being highest in the range 35\u201340 \u00b0C, when using an 80:20 water:ethanol solution. Hierarchical clustering showed three clusters, while factor analysis resulted in two factors and four groups of samples. In conclusion, it was found that extracts obtained from sweet cherry seeds have relevant bioactive compounds with applications in the food, pharmaceutical or cosmetic industries.<\/jats:p>","DOI":"10.3390\/agronomy12051227","type":"journal-article","created":{"date-parts":[[2022,5,20]],"date-time":"2022-05-20T04:27:22Z","timestamp":1653020842000},"page":"1227","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Extraction of Phenolic Compounds from Cherry Seeds: A Preliminary Study"],"prefix":"10.3390","volume":"12","author":[{"given":"Yuliya","family":"Dulyanska","sequence":"first","affiliation":[{"name":"CERNAS-IPV Research Centre and ESAV, Polytechnic Institute of Viseu, 3504-510 Viseu, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6502-7202","authenticated-orcid":false,"given":"Lu\u00edsa Paula","family":"Cruz-Lopes","sequence":"additional","affiliation":[{"name":"CERNAS-IPV Research Centre and ESTGV, Polytechnic Institute of Viseu, 3504-510 Viseu, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6660-3128","authenticated-orcid":false,"given":"Bruno","family":"Esteves","sequence":"additional","affiliation":[{"name":"CERNAS-IPV Research Centre and ESTGV, Polytechnic Institute of Viseu, 3504-510 Viseu, Portugal"}]},{"given":"Jos\u00e9 Vicente","family":"Ferreira","sequence":"additional","affiliation":[{"name":"CERNAS-IPV Research Centre and ESTGV, Polytechnic Institute of Viseu, 3504-510 Viseu, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4308-1563","authenticated-orcid":false,"given":"Idalina","family":"Domingos","sequence":"additional","affiliation":[{"name":"CERNAS-IPV Research Centre and ESTGV, Polytechnic Institute of Viseu, 3504-510 Viseu, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0597-7892","authenticated-orcid":false,"given":"Maria Jo\u00e3o","family":"Lima","sequence":"additional","affiliation":[{"name":"CERNAS-IPV Research Centre and ESAV, Polytechnic Institute of Viseu, 3504-510 Viseu, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2023-4475","authenticated-orcid":false,"given":"Paula M. R.","family":"Correia","sequence":"additional","affiliation":[{"name":"CERNAS-IPV Research Centre and ESAV, Polytechnic Institute of Viseu, 3504-510 Viseu, Portugal"}]},{"given":"Manuela","family":"Ferreira","sequence":"additional","affiliation":[{"name":"UICISA: E Research Centre and ESSV, Polytechnic Institute of Viseu, 3504-510 Viseu, Portugal"}]},{"given":"Anabela","family":"Fragata","sequence":"additional","affiliation":[{"name":"CI&DEI Research Centre and ESTGV, Polytechnic Institute of Viseu, 3504-510 Viseu, Portugal"}]},{"given":"Maria Jo\u00e3o","family":"Barroca","sequence":"additional","affiliation":[{"name":"Molecular Physical-Chemistry R&D Unit, Department of Chemistry, University of Coimbra, 3004-535 Coimbra, Portugal"},{"name":"Agrarian School, Polytechnic Institute of Coimbra, 3040-360 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5258-7980","authenticated-orcid":false,"given":"Aida","family":"Moreira da Silva","sequence":"additional","affiliation":[{"name":"Molecular Physical-Chemistry R&D Unit, Department of Chemistry, University of Coimbra, 3004-535 Coimbra, Portugal"},{"name":"Agrarian School, Polytechnic Institute of Coimbra, 3040-360 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0595-6805","authenticated-orcid":false,"given":"Raquel P. F.","family":"Guin\u00e9","sequence":"additional","affiliation":[{"name":"CERNAS-IPV Research Centre and ESAV, Polytechnic Institute of Viseu, 3504-510 Viseu, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2022,5,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1005","DOI":"10.21273\/HORTSCI13960-19","article-title":"Quality and Nutritional Compounds of Prunus cerasus L. Var. Austera Fruit Grown in Central Italy","volume":"54","author":"Proietti","year":"2019","journal-title":"HortScience"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1627","DOI":"10.1002\/jctb.5532","article-title":"Bioactive Compounds of Sweet and Sour Cherry Stems Obtained by Subcritical Water Extraction","volume":"93","author":"Clavijo","year":"2018","journal-title":"J. Chem. Technol. 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