{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T05:21:29Z","timestamp":1776835289216,"version":"3.51.2"},"reference-count":64,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2025,3,30]],"date-time":"2025-03-30T00:00:00Z","timestamp":1743292800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"FCT -Foundation for Science and Technology","doi-asserted-by":"publisher","award":["UIDB\/05183"],"award-info":[{"award-number":["UIDB\/05183"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Agronomy"],"abstract":"<jats:p>High temperatures significantly impact grapevine growth and development and lead to severe losses in grape quality and production. To minimize the impact of those environmental constraints, the application of biostimulants (BSts) has emerged as one of the most interesting strategies. BSts application derived from cactus species has been described as a successful approach to enhance tolerance to biotic and abiotic stresses. In this study, an aqueous extract prepared from the cladodes of Opuntia ficus-indica was applied through foliar spraying to grapevine plants (Vitis vinifera L.) \u2019Aragonez\u2019 already under heat stress. The effect of the extract application on protecting grapevine plants against heat stress was assessed in an experiment running during 15 days after extract application by determining several physiological parameters and detecting the changes in the whole proteome profile by comparing non-treated and extract-treated samples. Results show that physiological parameters directly related to photosynthesis showed a positive effect of the extract in mitigating heat stress in grapevines. Proteomic analysis indicated that the extract significantly upregulated proteins associated with photosynthesis and stress responses. This study provides new insights about the effect of O. ficus-indica extract in grapevines, offering a valuable strategy for future applications under field conditions.<\/jats:p>","DOI":"10.3390\/agronomy15040869","type":"journal-article","created":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T04:21:01Z","timestamp":1743481261000},"page":"869","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Exploring Opuntia ficus-indica as a Strategy to Mitigate High Temperatures Effects in Vineyards: Insights into Physiological and Proteomic Responses"],"prefix":"10.3390","volume":"15","author":[{"given":"L\u00e9nia","family":"Rodrigues","sequence":"first","affiliation":[{"name":"MED\u2014Mediterranean Institute for Agriculture, Environment and Development & CHANGE\u2014Global Change and Sustainability Institute, Institute for Advanced Studies and Research, Universidade de \u00c9vora, P\u00f3lo da Mitra, Ap. 94, 7006-554 \u00c9vora, Portugal"}]},{"given":"In\u00eas","family":"Santana","sequence":"additional","affiliation":[{"name":"Escola de Ci\u00eancias e Tecnologia, Universidade de \u00c9vora, Col\u00e9gio Lu\u00eds Ant\u00f3nio Verney, Rua Rom\u00e3o Ramalho, 59, 7000-671 \u00c9vora, Portugal"}]},{"given":"Renato","family":"Coelho","sequence":"additional","affiliation":[{"name":"MED\u2014Mediterranean Institute for Agriculture, Environment and Development & CHANGE\u2014Global Change and Sustainability Institute, Departamento de Biologia, Escola de Ci\u00eancias e Tecnologia, Universidade de \u00c9vora, P\u00f3lo da Mitra, Ap. 94, 7006-554 \u00c9vora, Portugal"}]},{"given":"Gabriela","family":"Murta","sequence":"additional","affiliation":[{"name":"MED\u2014Mediterranean Institute for Agriculture, Environment and Development & CHANGE\u2014Global Change and Sustainability Institute, Institute for Advanced Studies and Research, Universidade de \u00c9vora, P\u00f3lo da Mitra, Ap. 94, 7006-554 \u00c9vora, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3688-607X","authenticated-orcid":false,"given":"H\u00e9lia","family":"Cardoso","sequence":"additional","affiliation":[{"name":"MED\u2014Mediterranean Institute for Agriculture, Environment and Development & CHANGE\u2014Global Change and Sustainability Institute, Departamento de Biologia, Escola de Ci\u00eancias e Tecnologia, Universidade de \u00c9vora, P\u00f3lo da Mitra, Ap. 94, 7006-554 \u00c9vora, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5347-1338","authenticated-orcid":false,"given":"Catarina","family":"Campos","sequence":"additional","affiliation":[{"name":"MED\u2014Mediterranean Institute for Agriculture, Environment and Development & CHANGE\u2014Global Change and Sustainability Institute, Institute for Advanced Studies and Research, Universidade de \u00c9vora, P\u00f3lo da Mitra, Ap. 94, 7006-554 \u00c9vora, Portugal"}]},{"given":"Jo\u00e3o Mota","family":"Barroso","sequence":"additional","affiliation":[{"name":"MED\u2014Mediterranean Institute for Agriculture, Environment and Development & CHANGE\u2014Global Change and Sustainability Institute, Departamento de Fitotecnia, Escola de Ci\u00eancias e Tecnologia, Universidade de \u00c9vora, P\u00f3lo da Mitra, Ap. 94, 7006-554 \u00c9vora, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5267-3723","authenticated-orcid":false,"given":"Ana Elisa","family":"Rato","sequence":"additional","affiliation":[{"name":"MED\u2014Mediterranean Institute for Agriculture, Environment and Development & CHANGE\u2014Global Change and Sustainability Institute, Departamento de Fitotecnia, Escola de Ci\u00eancias e Tecnologia, Universidade de \u00c9vora, P\u00f3lo da Mitra, Ap. 94, 7006-554 \u00c9vora, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2025,3,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Santos, R.B., and Figueiredo, A. (2023). Biotic and Abiotic Stress Management in Grapevine: Recent Advances and Major Breakthroughs. Agronomy, 13.","DOI":"10.3390\/agronomy13061584"},{"key":"ref_2","first-page":"301","article-title":"Impacts of Drought and\/or Heat Stress on Physiological, Developmental, Growth, and Yield Processes of Crop Plants","volume":"1","author":"Prasad","year":"2008","journal-title":"Resp. Crops Limit. Water Underst. Model. Water Stress Eff. Plant Growth Process."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"565","DOI":"10.3923\/jbs.2010.565.572","article-title":"Impact of Heat Stress on Germination and Growth in Higher Plants: Physiological, Biochemical and Molecular Repercussions and Mechanisms of Defence","volume":"10","author":"Essemine","year":"2010","journal-title":"J. Biol. Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2063","DOI":"10.1016\/j.jplph.2011.06.009","article-title":"Photosynthesis Is Improved by Exogenous Calcium in Heat-Stressed Tobacco Plants","volume":"168","author":"Tan","year":"2011","journal-title":"J. Plant Physiol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"3043","DOI":"10.1021\/jf405772f","article-title":"Characterization of Grape (Vitis vinifera L.) Berry Sunburn Symptoms by Reflectance","volume":"62","author":"Rustioni","year":"2014","journal-title":"J. Agric. Food Chem."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Bhupenchandra, I., Chongtham, S.K., Devi, E.L., Ramesh, R., Choudhary, A.K., Salam, M.D., Sahoo, M.R., Bhutia, T.L., Devi, S.H., and Thounaojam, A.S. (2022). Role of Biostimulants in Mitigating the Effects of Climate Change on Crop Performance. Front. Plant Sci., 13.","DOI":"10.3389\/fpls.2022.967665"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"261","DOI":"10.2135\/cropsci2006.03.0171","article-title":"Effects of a Biostimulant on the Heat Tolerance Associated with Photosynthetic Capacity, Membrane Thermostability, and Polyphenol Production of Perennial Ryegrass","volume":"47","author":"Kauffman","year":"2007","journal-title":"Crop Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.scienta.2015.09.021","article-title":"Plant Biostimulants: Definition, Concept, Main Categories and Regulation","volume":"196","year":"2015","journal-title":"Sci. Hortic."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Castiglione, A.M., Mannino, G., Contartese, V., Bertea, C.M., and Ertani, A. (2021). Microbial Biostimulants as Response to Modern Agriculture Needs: Composition, Role and Application of These Innovative Products. Plants, 10.","DOI":"10.3390\/plants10081533"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Hasanuzzaman, M., Parvin, K., Bardhan, K., Nahar, K., Anee, T.I., Masud, A.A.C., and Fotopoulos, V. (2021). Biostimulants for the Regulation of Reactive Oxygen Species Metabolism in Plants under Abiotic Stress. Cells, 10.","DOI":"10.3390\/cells10102537"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1007\/s42976-022-00299-6","article-title":"Biostimulants and Their Role in Improving Plant Growth under Drought and Salinity","volume":"51","author":"Rakkammal","year":"2023","journal-title":"Cereal Res. Commun."},{"key":"ref_12","unstructured":"Khurana, A., and Kumar, V. (2022). State of Biofertilizers and Organic Fertilizers in India, Centre for Science and Environment."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Van Oosten, M.J., Pepe, O., De Pascale, S., Silletti, S., and Maggio, A. (2017). The Role of Biostimulants and Bioeffectors as Alleviators of Abiotic Stress in Crop Plants. Chem. Biol. Technol. Agric., 4.","DOI":"10.1186\/s40538-017-0089-5"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Monteiro, E., Gon\u00e7alves, B., Cortez, I., and Castro, I. (2022). The Role of Biostimulants as Alleviators of Biotic and Abiotic Stresses in Grapevine: A Review. Plants, 11.","DOI":"10.3390\/plants11030396"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Shukla, P.S., Mantin, E.G., Adil, M., Bajpai, S., Critchley, A.T., and Prithiviraj, B. (2019). Ascophyllum nodosum-Based Biostimulants: Sustainable Applications in Agriculture for the Stimulation of Plant Growth, Stress Tolerance, and Disease Management. Front. Plant Sci., 10.","DOI":"10.3389\/fpls.2019.00655"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Cataldo, E., Fucile, M., and Mattii, G.B. (2022). Biostimulants in Viticulture: A Sustainable Approach against Biotic and Abiotic Stresses. Plants, 11.","DOI":"10.3390\/plants11020162"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Irani, H., ValizadehKaji, B., and Naeini, M.R. (2021). Biostimulant-Induced Drought Tolerance in Grapevine Is Associated with Physiological and Biochemical Changes. Chem. Biol. Technol. Agric., 8.","DOI":"10.1186\/s40538-020-00200-9"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Olavarrieta, C.E., Sampedro, M.C., Vallejo, A., \u0160tefelov\u00e1, N., Barrio, R.J., and De Diego, N. (2022). Biostimulants as an Alternative to Improve the Wine Quality from Vitis vinifera (Cv. Tempranillo) in La Rioja. Plants, 11.","DOI":"10.3390\/plants11121594"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Samuels, L.J., Setati, M.E., and Blancquaert, E.H. (2022). Towards a Better Understanding of the Potential Benefits of Seaweed Based Biostimulants in Vitis vinifera L. Cultivars. Plants, 11.","DOI":"10.3390\/plants11030348"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1016\/j.foodres.2013.11.004","article-title":"Oak Extract Application to Grapevines as a Plant Biostimulant to Increase Wine Polyphenols","volume":"55","author":"Pardo","year":"2014","journal-title":"Food Res. Int."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Zhang, Q., and White, J.F. (2021). Bioprospecting Desert Plants for Endophytic and Biostimulant Microbes: A Strategy for Enhancing Agricultural Production in a Hotter, Drier Future. Biology, 10.","DOI":"10.3390\/biology10100961"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"136756","DOI":"10.1016\/j.foodchem.2023.136756","article-title":"Composition of Discarded Sicilian Fruits of Opuntia ficus indica L.: Phenolic Content, Mineral Profile and Antioxidant Activity in Peel, Seeds and Whole Fruit","volume":"428","author":"Bellumori","year":"2023","journal-title":"Food Chem."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1016\/j.procbio.2023.07.016","article-title":"Effect of Enzymatic Treatments and Microfiltration on the Physicochemical Properties and Antioxidant Activities of Two Tunisian Prickly Pear Juices","volume":"132","author":"Kallel","year":"2023","journal-title":"Process Biochem."},{"key":"ref_24","unstructured":"Murta, G., Rato, A.E., Martins, M.d.R., and Coelho, R. (2023, January 11\u201312). Application of Opuntia ficus indica Extracts on Grapevine to Mitigate the Effects of Climate Change. Proceedings of the VIII PhD Students Meeting in Environment and Agriculture, Universidade de \u00c9vora, Evora, Portugal."},{"key":"ref_25","unstructured":"Pedroso, V., Martins, S., Brites, J., and Lopes, C. (1960). Efeito Do Porta-Enxerto No Vigor, Rendimento e Qualidade Do Mosto Da Casta Touriga Nacional Na Regi\u00e3o Do D\u00e3o. Contributions to Probability and Statistics: Essays in Honor of Harold Hotelling, de Vitivinicultura do Alentejo, ATEVA\/CVRA."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/0003-2697(84)90782-6","article-title":"A Method for the Quantitative Recovery of Protein in Dilute Solution in the Presence of Detergents and Lipids","volume":"138","author":"Wessel","year":"1984","journal-title":"Anal. Biochem."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Walker, J.M. (1988). The Bradford Method for Protein Quantitation. New Protein Techniques, Humana Press.","DOI":"10.1385\/0896031268"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4","volume":"227","author":"Laemmli","year":"1970","journal-title":"Nature"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1093\/biomet\/52.3-4.591","article-title":"An Analysis of Variance Test for Normality (Complete Samples)","volume":"52","author":"Shapiro","year":"1965","journal-title":"Biometrika"},{"key":"ref_30","unstructured":"Levene, H. (1960). Robust Tests for Equality of Variances. Contrib. Probab. Stat., 278\u2013292."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1111\/nph.15899","article-title":"How Do Stomata Respond to Water Status?","volume":"224","author":"Buckley","year":"2019","journal-title":"New Phytol."},{"key":"ref_32","first-page":"2314","article-title":"Diurnal Variation in Grapevine Water Stress as a Function of Changing Soil Water Status and Meteorological Conditions","volume":"8","year":"2017","journal-title":"SAJEV S. Afr. J. Enol. Vitic."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1007\/s40626-016-0074-6","article-title":"Adaptive Strategies of Two Mediterranean Grapevines Varieties (Aragonez syn. Tempranillo and Trincadeira) Face Drought: Physiological and Structural Responses","volume":"28","author":"Vaz","year":"2016","journal-title":"Theor. Exp. Plant Physiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1115","DOI":"10.1590\/S1415-43662011001100002","article-title":"Potencial de \u00e1gua no solo e na folha da videira \u201cSugraone\u201d sob d\u00e9ficit h\u00eddrico","volume":"15","author":"Marinho","year":"2011","journal-title":"Rev. Bras. Eng. Agr\u00edcola Ambient."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1111\/j.1755-0238.2011.00125.x","article-title":"Grapevine Canopy Response to a High-Temperature Event during Deficit Irrigation","volume":"17","author":"Edwards","year":"2011","journal-title":"Aust. J. Grape Wine Res."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Melo, G.B., da Silva, A.G., da Costa, A.C., Alves da Silva, A., Rosa, M., Bessa, L.A., Rodrigues, C.R., Castoldi, G., and Vitorino, L.C. (2024). Foliar Application of Biostimulant Mitigates Water Stress Effects on Soybean. Agronomy, 14.","DOI":"10.3390\/agronomy14030414"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Venios, X., Korkas, E., Nisiotou, A., and Banilas, G. (2020). Grapevine Responses to Heat Stress and Global Warming. Plants, 9.","DOI":"10.3390\/plants9121754"},{"key":"ref_38","unstructured":"Dubeux, J. (2011). Use of Cactus for Livestock Feeding, Universidad Federal Rural de Pernambuco (UFRPE)."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Abbas, E.Y., Ezzat, M.I., El Hefnawy, H.M., and Abdel-Sattar, E. (2022). An Overview and Update on the Chemical Composition and Potential Health Benefits of Opuntia ficus-indica (L.) Miller. J. Food Biochem., 46.","DOI":"10.1111\/jfbc.14310"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1002\/mnfr.200400071","article-title":"Cactus Stems (Opuntia spp.): A Review on Their Chemistry, Technology, and Uses","volume":"49","author":"Stintzing","year":"2005","journal-title":"Mol. Nutr. Food Res."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1016\/j.ijbiomac.2016.07.042","article-title":"Extraction and Characterization of Three Polysaccharides Extracted from Opuntia ficus indica Cladodes","volume":"92","author":"Bayar","year":"2016","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1007\/s11099-005-0173-0","article-title":"Effect of Water Deficit on Photosynthetic and Other Physiological Responses in Grapevine (Vitis vinifera L. Cv. Riesling) Plants","volume":"44","author":"Bertamini","year":"2006","journal-title":"Photosynthetica"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1007\/s11738-007-0073-y","article-title":"Drought Response of Two Bedding Plants","volume":"29","author":"Kutnik","year":"2007","journal-title":"Acta Physiol. Plant."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1023\/A:1022390917656","article-title":"Influence of Brassinosteroids on Antioxidant Enzymes Activity in Tomato Under Different Temperatures","volume":"45","author":"Mazorra","year":"2002","journal-title":"Biol. Plant."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1080\/14620316.2015.1123405","article-title":"Sana Physiological Responses of Two Grapevine (Vitis vinifera L.) Cultivars to CycocelTM Treatment during Drought","volume":"91","author":"Abdi","year":"2016","journal-title":"J. Hortic. Sci. Biotechnol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.plaphy.2018.11.037","article-title":"Alleviation of Drought Stress in Grapevine by Foliar-Applied Strigolactones","volume":"135","author":"Min","year":"2019","journal-title":"Plant Physiol. Biochem."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Fahim, S., Ghanbari, A., Naji, A.M., Shokohian, A.A., Maleki Lajayer, H., Gohari, G., and Hano, C. (2022). Multivariate Discrimination of Some Grapevine Cultivars under Drought Stress in Iran. Horticulturae, 8.","DOI":"10.3390\/horticulturae8100871"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1515\/fhort-2017-0021","article-title":"Morphological and Physiological Responses of Grapevine (Vitis vinifera L.) to Drought Stress and Dust Pollution","volume":"29","author":"Karami","year":"2017","journal-title":"Folia Hortic."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"113383","DOI":"10.1016\/j.scienta.2024.113383","article-title":"Investigation of Some Physiological and Chemical Changes in Shoots and Leaves Caused by UV-C Radiation as an Abiotic Stress Source in Grapevine Cuttings","volume":"336","author":"Tahmaz","year":"2024","journal-title":"Sci. Hortic."},{"key":"ref_50","first-page":"601","article-title":"The Physiological Response of Three Iranian Grape Cultivars to Progressive Drought Stress","volume":"13","author":"Ghaderi","year":"2011","journal-title":"J. Agr. Sci. Tech"},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Meggio, F., Trevisan, S., Manoli, A., Ruperti, B., and Quaggiotti, S. (2020). Systematic Investigation of the Effects of a Novel Protein Hydrolysate on the Growth, Physiological Parameters, Fruit Development and Yield of Grapevine (Vitis vinifera L., Cv Sauvignon Blanc) under Water Stress Conditions. Agronomy, 10.","DOI":"10.3390\/agronomy10111785"},{"key":"ref_52","first-page":"20","article-title":"Changes in leaf and shoot water statutes of grapevines in response to contrasting water availability and glycine betaine pulverization","volume":"1","author":"Jalil","year":"2017","journal-title":"Int. J. Agric. Environ. Food Sci."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Wu, J., Zhong, H., Ma, Y., Bai, S., Yadav, V., Zhang, C., Zhang, F., Shi, W., Abudureheman, R., and Wang, X. (2024). Effects of Different Biostimulants on Growth and Development of Grapevine Seedlings under High-Temperature Stress. Horticulturae, 10.","DOI":"10.3390\/horticulturae10030269"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1080\/14620316.2016.1211493","article-title":"Photosynthetic and Physiological Responses to High Temperature in Grapevine (Vitis vinifera L.) Leaves during the Seedling Stage","volume":"92","author":"Xiao","year":"2017","journal-title":"J. Hortic. Sci. Biotechnol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"109413","DOI":"10.1016\/j.scienta.2020.109413","article-title":"Protein Hydrolysates Modulate Leaf Proteome and Metabolome in Water-Stressed Grapevines","volume":"270","author":"Bavaresco","year":"2020","journal-title":"Sci. Hortic."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"153240","DOI":"10.1016\/j.jplph.2020.153240","article-title":"Rubisco Carboxylase\/Oxygenase: From the Enzyme to the Globe: A Gas Exchange Perspective","volume":"252","year":"2020","journal-title":"J. Plant Physiol."},{"key":"ref_57","unstructured":"Hatch, M.D., and Boardman, N.K. (1987). 3\u2014Rubisco: Structure, Mechanisms, and Prospects for Improvement. Photosynthesis, Academic Press."},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Liu, G.-T., Ma, L., Duan, W., Wang, B.-C., Li, J.-H., Xu, H.-G., Yan, X.-Q., Yan, B.-F., Li, S.-H., and Wang, L.-J. (2014). Differential Proteomic Analysis of Grapevine Leaves by iTRAQ Reveals Responses to Heat Stress and Subsequent Recovery. BMC Plant Biol., 14.","DOI":"10.1186\/1471-2229-14-110"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1016\/j.jplph.2016.11.016","article-title":"Changes in the Proteome of Grapevine Leaves (Vitis vinifera L.) during Long-Term Drought Stress","volume":"211","author":"Weidner","year":"2017","journal-title":"J. Plant Physiol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1007\/s10725-020-00586-4","article-title":"Physiological and Proteomic Responses to Drought Stress in Leaves of Two Wild Grapevines (Vitis sylvestris): A Comparative Study","volume":"91","author":"Azri","year":"2020","journal-title":"Plant Growth Regul."},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Ilangumaran, G., Subramanian, S., and Smith, D.L. (2022). Soybean Leaf Proteomic Profile Influenced by Rhizobacteria Under Optimal and Salt Stress Conditions. Front. Plant Sci., 13.","DOI":"10.3389\/fpls.2022.809906"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1007\/BF02922990","article-title":"\u03b1- and \u03b2-Amylases in Seed Germination","volume":"19","author":"Goswami","year":"1977","journal-title":"Biol. Plant."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1146\/annurev.pp.42.060191.003051","article-title":"The Roles of Heat Shock Proteins in Plants","volume":"42","author":"Vierling","year":"1991","journal-title":"Annu. Rev. Plant. Physiol. Plant. Mol. Biol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"104337","DOI":"10.1016\/j.jprot.2021.104337","article-title":"Fulvic Acid Enhances Drought Resistance in Tea Plants by Regulating the Starch and Sucrose Metabolism and Certain Secondary Metabolism","volume":"247","author":"Qiu","year":"2021","journal-title":"J. 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