{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T19:41:47Z","timestamp":1773171707657,"version":"3.50.1"},"reference-count":75,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2020,2,29]],"date-time":"2020-02-29T00:00:00Z","timestamp":1582934400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"FEDER funds through the Operational Competitiveness Program (COMPETE)","award":["POCI-01-0247-FEDER-017616"],"award-info":[{"award-number":["POCI-01-0247-FEDER-017616"]}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia\/MCTES","award":["UID\/QUI\/50006\/2019"],"award-info":[{"award-number":["UID\/QUI\/50006\/2019"]}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia\/MCTES","award":["PD\/BDE\/114427\/2016"],"award-info":[{"award-number":["PD\/BDE\/114427\/2016"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Zinc is an essential element, a cofactor of many enzymes, and performs catalytic, structural and regulatory functions. Once in the gastrointestinal tract, zinc can interact with food constituents. Phytic acid, the major phosphorus storage in plants, limits zinc availability from animal feeds due to the formation of insoluble complexes with phytates. This study tested the effect of supplemental zinc source (zinc sulfate and a chelate zinc proteinate) and the addition of exogenous enzymes from a solid-state fermentation product of Aspergillus niger to a high phytate diet. The study was designed according to three Latin Squares 4 \u00d7 4 with a 2 \u00d7 2 factorial arrangement of treatments, with four periods, four diets, and 12 young adult Beagles. Periods lasted 5 weeks each. Diets were supplemented with 75 mg\/kg of zinc sulfate (IZ) or zinc proteinate (OZ), and without or with 200 mg\/kg of exogenous enzymes (IZ+, OZ+). Results showed that zinc proteinate increased the bioavailability of phosphorus, yet the zinc biomarkers remained unaffected by the zinc source, with the exception of lymphocyte subsets that benefit from zinc proteinate. The use of exogenous enzymes did not affect zinc availability nor nutrient and energy digestibility.<\/jats:p>","DOI":"10.3390\/ani10030400","type":"journal-article","created":{"date-parts":[[2020,3,2]],"date-time":"2020-03-02T07:35:30Z","timestamp":1583134530000},"page":"400","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Effect of Zinc Source and Exogenous Enzymes Supplementation on Zinc Status in Dogs Fed High Phytate Diets"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0179-3044","authenticated-orcid":false,"given":"Ana Margarida","family":"Pereira","sequence":"first","affiliation":[{"name":"LAQV, REQUIMTE, Instituto de Ci\u00eancias Biom\u00e9dicas de Abel Salazar, Universidade do Porto, R. Jorge Viterbo Ferreira n\u00b0 228, 4050-313 Porto, Portugal"}]},{"given":"Margarida","family":"Guedes","sequence":"additional","affiliation":[{"name":"LAQV, REQUIMTE, Instituto de Ci\u00eancias Biom\u00e9dicas de Abel Salazar, Universidade do Porto, R. Jorge Viterbo Ferreira n\u00b0 228, 4050-313 Porto, Portugal"}]},{"given":"Elisabete","family":"Matos","sequence":"additional","affiliation":[{"name":"SORGAL, Sociedade de \u00d3leos e Ra\u00e7\u00f5es S.A., Estrada Nacional 109 Lugar da Pardala, 3880-728 S. Jo\u00e3o Ovar, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8021-4783","authenticated-orcid":false,"given":"Edgar","family":"Pinto","sequence":"additional","affiliation":[{"name":"LAQV, REQUIMTE, Departamento de Ci\u00eancias Qu\u00edmicas, Faculdade de Farm\u00e1cia, Universidade do Porto, R. Jorge Viterbo Ferreira n\u00b0 228, 4050-313 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1297-3198","authenticated-orcid":false,"given":"Agostinho A.","family":"Almeida","sequence":"additional","affiliation":[{"name":"LAQV, REQUIMTE, Departamento de Ci\u00eancias Qu\u00edmicas, Faculdade de Farm\u00e1cia, Universidade do Porto, R. Jorge Viterbo Ferreira n\u00b0 228, 4050-313 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2938-0214","authenticated-orcid":false,"given":"Marcela A.","family":"Segundo","sequence":"additional","affiliation":[{"name":"LAQV, REQUIMTE, Departamento de Ci\u00eancias Qu\u00edmicas, Faculdade de Farm\u00e1cia, Universidade do Porto, R. Jorge Viterbo Ferreira n\u00b0 228, 4050-313 Porto, Portugal"}]},{"given":"Alexandra","family":"Correia","sequence":"additional","affiliation":[{"name":"i3S\/IBMC\u2014Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade\/Instituto de Biologia Molecular e Celular, Universidade do Porto, R. Alfredo Allen, 4200-135 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7007-306X","authenticated-orcid":false,"given":"Manuel","family":"Vilanova","sequence":"additional","affiliation":[{"name":"i3S\/IBMC\u2014Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade\/Instituto de Biologia Molecular e Celular, Universidade do Porto, R. Alfredo Allen, 4200-135 Porto, Portugal"},{"name":"Instituto de Ci\u00eancias Biom\u00e9dicas de Abel Salazar, Universidade do Porto, R. Jorge Viterbo Ferreira n\u00b0 228, 4050-313 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6237-417X","authenticated-orcid":false,"given":"Ant\u00f3nio J. M.","family":"Fonseca","sequence":"additional","affiliation":[{"name":"LAQV, REQUIMTE, Instituto de Ci\u00eancias Biom\u00e9dicas de Abel Salazar, Universidade do Porto, R. Jorge Viterbo Ferreira n\u00b0 228, 4050-313 Porto, Portugal"}]},{"given":"Ana Rita J.","family":"Cabrita","sequence":"additional","affiliation":[{"name":"LAQV, REQUIMTE, Instituto de Ci\u00eancias Biom\u00e9dicas de Abel Salazar, Universidade do Porto, R. Jorge Viterbo Ferreira n\u00b0 228, 4050-313 Porto, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2020,2,29]]},"reference":[{"key":"ref_1","unstructured":"NRC (2006). Nutrient Requirements of Dogs and Cats, National Academies Press."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.applanim.2016.04.012","article-title":"Comparison of serum trace elements and antioxidant levels in terrier dogs with or without behavior problems","volume":"180","author":"Soltanian","year":"2016","journal-title":"Appl. Anim. Behav. 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