{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T03:04:36Z","timestamp":1760238276908,"version":"build-2065373602"},"reference-count":53,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2020,7,29]],"date-time":"2020-07-29T00:00:00Z","timestamp":1595980800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Objective: This work aimed to determine the carriage rate of ESBL-producing Escherichia coli as well as their genetic characteristics in camels from the Canary Islands, Spain. Methods: Fecal samples were recovered from 58 healthy camels from Gran Canaria (n = 32) and Fuerteventura Islands (n = 26) during July 2019. They were seeded on MacConkey (MC) agar no supplemented and supplemented (MC + CTX) with cefotaxime (2 \u00b5g\/mL). Antimicrobial susceptibility was determined by disk diffusion test (CLSI, 2018). The presence of blaCTX-M, blaSHV, blaTEM,blaCMY-2 and blaOXA-1\/48 genes was tested by PCR\/sequencing. Furthermore, the mcr-1 (colistin resistance), tetA\/tetB (tetracycline resistance), int1 (integrase of class 1 integrons) and stx1,2 genes were analyzed. Phylogenetic groups and sequence types were determined by specific-PCR\/sequencing for selected isolates. Results: E. coli was obtained from all the 58 camels in MC media (100%) and in five of them in MC + CTX media (8.6%). Furthermore, 63.8% of E. coli isolates recovered from MC agar were susceptible to all the antibiotics tested. The five E. coli isolates recovered from MC + CTX media were characterized and two of them were ESBL-producers (3.4%). Both ESBL-producer isolates carried the blaCTX-M-15 gene and belonged to the lineages ST3018 (phylogroup A) and ST69 (phylogroup B1). The 3 ESBL-negative isolates recovered from MC-CTX plates were ascribed to phylogroup-B1. Conclusions: Camels can be a source of ESBL-producer bacteria, containing the widespread blaCTX-M-15 gene associated with the lineages ST3018 and ST69.<\/jats:p>","DOI":"10.3390\/ani10081295","type":"journal-article","created":{"date-parts":[[2020,7,30]],"date-time":"2020-07-30T03:36:38Z","timestamp":1596080198000},"page":"1295","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Escherichia coli Producing Extended-Spectrum \u03b2-lactamases (ESBL) from Domestic Camels in the Canary Islands: A One Health Approach"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1762-412X","authenticated-orcid":false,"given":"Isabel","family":"Carvalho","sequence":"first","affiliation":[{"name":"Microbiology and Antibiotic Resistance Team (MicroART), Department of Veterinary Sciences, University of Tr\u00e1s-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal"},{"name":"Department of Genetics and Biotechnology, UTAD, 5000-801 Vila Real, Portugal"},{"name":"Functional Genomics and Proteomics Unit, UTAD, 5000-801 Vila Real, Portugal"},{"name":"Laboratory Associated for Green Chemistry (LAQV-REQUIMTE), New University of Lisbon, 2829-516 Monte da Caparica, Portugal"},{"name":"Area Biochemistry and Molecular Biology, University of La Rioja, 26006 Logro\u00f1o, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2387-1426","authenticated-orcid":false,"given":"Mar\u00eda Teresa","family":"Tejedor-Junco","sequence":"additional","affiliation":[{"name":"Research Institute of Biomedical and Health Sciences, University of Las Palmas de Gran Canaria, 35001 Canary Islands, Spain"},{"name":"Department of Clinical Sciences, University of Las Palmas de Gran Canaria, 35001 Canary Islands, Spain"}]},{"given":"Margarita","family":"Gonz\u00e1lez-Mart\u00edn","sequence":"additional","affiliation":[{"name":"Research Institute of Biomedical and Health Sciences, University of Las Palmas de Gran Canaria, 35001 Canary Islands, Spain"},{"name":"Department of Clinical Sciences, University of Las Palmas de Gran Canaria, 35001 Canary Islands, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7812-2065","authenticated-orcid":false,"given":"Juan Alberto","family":"Corbera","sequence":"additional","affiliation":[{"name":"Research Institute of Biomedical and Health Sciences, University of Las Palmas de Gran Canaria, 35001 Canary Islands, Spain"},{"name":"Department of Animal Pathology, Veterinary School, University of Las Palmas de Gran Canaria, 35001 Canary Islands, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9406-8433","authenticated-orcid":false,"given":"Vanessa","family":"Silva","sequence":"additional","affiliation":[{"name":"Microbiology and Antibiotic Resistance Team (MicroART), Department of Veterinary Sciences, University of Tr\u00e1s-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal"},{"name":"Department of Genetics and Biotechnology, UTAD, 5000-801 Vila Real, Portugal"},{"name":"Functional Genomics and Proteomics Unit, UTAD, 5000-801 Vila Real, Portugal"},{"name":"Laboratory Associated for Green Chemistry (LAQV-REQUIMTE), New University of Lisbon, 2829-516 Monte da Caparica, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6365-0735","authenticated-orcid":false,"given":"Gilberto","family":"Igrejas","sequence":"additional","affiliation":[{"name":"Department of Genetics and Biotechnology, UTAD, 5000-801 Vila Real, Portugal"},{"name":"Functional Genomics and Proteomics Unit, UTAD, 5000-801 Vila Real, Portugal"},{"name":"Laboratory Associated for Green Chemistry (LAQV-REQUIMTE), New University of Lisbon, 2829-516 Monte da Caparica, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3709-1690","authenticated-orcid":false,"given":"Carmen","family":"Torres","sequence":"additional","affiliation":[{"name":"Area Biochemistry and Molecular Biology, University of La Rioja, 26006 Logro\u00f1o, Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0925-689X","authenticated-orcid":false,"given":"Patr\u00edcia","family":"Poeta","sequence":"additional","affiliation":[{"name":"Microbiology and Antibiotic Resistance Team (MicroART), Department of Veterinary Sciences, University of Tr\u00e1s-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal"},{"name":"Laboratory Associated for Green Chemistry (LAQV-REQUIMTE), New University of Lisbon, 2829-516 Monte da Caparica, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2020,7,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/j.anaerobe.2014.09.009","article-title":"Azorean wild rabbits as reservoirs of antimicrobial resistant Escherichia coli","volume":"30","author":"Marinho","year":"2014","journal-title":"Anaerobe"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1089\/mdr.2008.0795","article-title":"Mechanisms of antibiotic resistance in Escherichia 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