{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T04:25:45Z","timestamp":1771302345338,"version":"3.50.1"},"reference-count":45,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2020,9,14]],"date-time":"2020-09-14T00:00:00Z","timestamp":1600041600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["POCI-01-0145-FEDER-029724"],"award-info":[{"award-number":["POCI-01-0145-FEDER-029724"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Breast cancer is a health problem that affects individual life quality and the family system. It is the most frequent type of cancer in women, but men are also affected. As an integrative approach, comparative oncology offers an opportunity to learn more about natural cancers in different species. Methods based on Raman spectroscopy have shown significant potential in the study of the human breast through the fingerprinting of biological tissue, which provides valuable information that can be used to identify, characterize and discriminate structures in breast tissue, in both healthy and carcinogenic environments. One of the most important applications of Raman spectroscopy in medical diagnosis is the characterization of microcalcifications, which are highly important diagnostic indicators of breast tissue diseases. Raman spectroscopy has been used to analyze the chemical composition of microcalcifications. These occur in benign and malignant lesions in the human breast, and Raman helps to discriminate microcalcifications as type I and type II according to their composition. This paper demonstrates the recent progress in understanding how this vibrational technique can discriminate through the fingerprint regions of lesions in unstained histology sections from canine mammary glands.<\/jats:p>","DOI":"10.3390\/ani10091652","type":"journal-article","created":{"date-parts":[[2020,9,14]],"date-time":"2020-09-14T20:51:12Z","timestamp":1600116672000},"page":"1652","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Discrimination of Benign and Malignant Lesions in Canine Mammary Tissue Samples Using Raman Spectroscopy: A Pilot Study"],"prefix":"10.3390","volume":"10","author":[{"given":"Diana","family":"Dantas","sequence":"first","affiliation":[{"name":"IFIMUP, Department of Physics and Astronomy, Faculty of Sciences of University of Porto, Rua do Campo Alegre 687, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1828-4633","authenticated-orcid":false,"given":"Liliana","family":"Soares","sequence":"additional","affiliation":[{"name":"IFIMUP, Department of Physics and Astronomy, Faculty of Sciences of University of Porto, Rua do Campo Alegre 687, 4169-007 Porto, Portugal"}]},{"given":"Susana","family":"Novais","sequence":"additional","affiliation":[{"name":"INESC TEC\u2014Institute for Systems and Computer Engineering, Technology and Science, Rua do Campo Alegre 687, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6104-9834","authenticated-orcid":false,"given":"Rui","family":"Vilarinho","sequence":"additional","affiliation":[{"name":"IFIMUP, Department of Physics and Astronomy, Faculty of Sciences of University of Porto, Rua do Campo Alegre 687, 4169-007 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4659-7503","authenticated-orcid":false,"given":"J. Agostinho","family":"Moreira","sequence":"additional","affiliation":[{"name":"IFIMUP, Department of Physics and Astronomy, Faculty of Sciences of University of Porto, Rua do Campo Alegre 687, 4169-007 Porto, Portugal"}]},{"given":"Susana","family":"Silva","sequence":"additional","affiliation":[{"name":"INESC TEC\u2014Institute for Systems and Computer Engineering, Technology and Science, Rua do Campo Alegre 687, 4169-007 Porto, Portugal"}]},{"given":"Orlando","family":"Fraz\u00e3o","sequence":"additional","affiliation":[{"name":"INESC TEC\u2014Institute for Systems and Computer Engineering, Technology and Science, Rua do Campo Alegre 687, 4169-007 Porto, Portugal"}]},{"given":"Teresa","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Veterin\u00e1ria; Escola de Ci\u00eancias e Tecnologia; Universidade de \u00c9vora; P\u00f3lo da Mitra, Apartado 94, 7002-554 \u00c9vora, Portugal"}]},{"given":"Nuno","family":"Leal","sequence":"additional","affiliation":[{"name":"DNAtech Laborat\u00f3rio Veterin\u00e1rio, Estrada do Pa\u00e7o do Lumiar, N.\u00b0 22 Edif\u00edcio E, 1\u00b0 Andar, 1649-038 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3922-3602","authenticated-orcid":false,"given":"Pedro","family":"Fa\u00edsca","sequence":"additional","affiliation":[{"name":"DNAtech Laborat\u00f3rio Veterin\u00e1rio, Estrada do Pa\u00e7o do Lumiar, N.\u00b0 22 Edif\u00edcio E, 1\u00b0 Andar, 1649-038 Lisboa, Portugal"},{"name":"Centro de Investiga\u00e7\u00e3o em BioCi\u00eancias e Tecnologias da Sa\u00fade, Faculdade de Medicina Veterin\u00e1ria, Universidade Lus\u00f3fona de Humanidades e Tecnologias, Campo Grande 376, 1749-024 Lisboa, Portugal"}]},{"given":"Joana","family":"Reis","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Veterin\u00e1ria; Escola de Ci\u00eancias e Tecnologia; Universidade de \u00c9vora; P\u00f3lo da Mitra, Apartado 94, 7002-554 \u00c9vora, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2020,9,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"11","DOI":"10.2147\/BCTT.S240928","article-title":"Coping assessment tools in the family caregivers of patients with breast cancer: A systematic review","volume":"12","author":"Noveiri","year":"2020","journal-title":"Breast Cancer Targets Ther."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1080\/05704928.2019.1601105","article-title":"Raman spectroscopy of breast cancer","volume":"55","author":"Shaaban","year":"2020","journal-title":"Appl. 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