{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T15:39:13Z","timestamp":1778341153726,"version":"3.51.4"},"reference-count":43,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2021,4,16]],"date-time":"2021-04-16T00:00:00Z","timestamp":1618531200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>In order to investigate the effect of a dietary amino acid mixture supplementation in lipopolysaccharide (LPS)-challenged weaned piglets, twenty-seven 28-day-old (8.2 \u00b1 1.0 kg) newly weaned piglets were randomly allocated to one of three experimental treatments for five weeks. Diet 1: a CTRL treatment. Diet 2: an LPS treatment, where piglets were intraperitoneally administered LPS (25 \u03bcg\/kg) on day 7. Diet 3: an LPS+MIX treatment, where piglets were intraperitoneally administered LPS on day 7 and fed a diet supplemented with a mixture of 0.3% of arginine, branched-chain amino acids (leucine, valine, and isoleucine), and cystine (MIX). Blood samples were drawn on day 10 and day 35, and serum was analysed for selected chemical parameters and proteomics. The LPS and LPS+MIX groups exhibited an increase in haptoglobin concentrations on day 10. The LPS group showed an increased cortisol concentration, while this concentration was reduced in the LPS+MIX group compared to the control group. Similarly, the LPS+MIX group showed a decreased haptoglobin concentration on day 35 compared to the two other groups. Immunoglobulin concentrations were affected by treatments. Indeed, on day 10, the concentrations of IgG and IgM were decreased by the LPS challenge, as illustrated by the lower concentrations of these two immunoglobulins in the LPS group compared to the control group. In addition, the supplementation with the amino acid mixture in the LPS+MIX further decreased IgG and increased IgM concentrations compared to the LPS group. Although a proteomics approach did not reveal important alterations in the protein profile in response to treatments, LPS-challenged piglets had an increase in proteins linked to the immune response, when compared to piglets supplemented with the amino acid mixture. Overall, data indicate that LPS-challenged piglets supplemented with this amino acid mixture are more protected against the detrimental effects of LPS.<\/jats:p>","DOI":"10.3390\/ani11041143","type":"journal-article","created":{"date-parts":[[2021,4,16]],"date-time":"2021-04-16T10:20:11Z","timestamp":1618568411000},"page":"1143","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Influence of Dietary Supplementation with an Amino Acid Mixture on Inflammatory Markers, Immune Status and Serum Proteome in LPS-Challenged Weaned Piglets"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1032-5987","authenticated-orcid":false,"given":"Jos\u00e9","family":"Prates","sequence":"first","affiliation":[{"name":"CIISA\u2014Centro de Investiga\u00e7\u00e3o Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterin\u00e1ria, Universidade de Lisboa, Avenida da Universidade T\u00e9cnica, Alto da Ajuda, 1300-477 Lisboa, Portugal"}]},{"given":"Jo\u00e3o","family":"Freire","sequence":"additional","affiliation":[{"name":"LEAF\u2014Linking Engineering, Agriculture and Food, Departamento de Ci\u00eancias e Engenharia de Biossistemas, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017 Lisboa, Portugal"}]},{"given":"Andr\u00e9","family":"de Almeida","sequence":"additional","affiliation":[{"name":"LEAF\u2014Linking Engineering, Agriculture and Food, Departamento de Ci\u00eancias e Engenharia de Biossistemas, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1017-3269","authenticated-orcid":false,"given":"C\u00e1tia","family":"Martins","sequence":"additional","affiliation":[{"name":"CIISA\u2014Centro de Investiga\u00e7\u00e3o Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterin\u00e1ria, Universidade de Lisboa, Avenida da Universidade T\u00e9cnica, Alto da Ajuda, 1300-477 Lisboa, Portugal"},{"name":"LEAF\u2014Linking Engineering, Agriculture and Food, Departamento de Ci\u00eancias e Engenharia de Biossistemas, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0990-7739","authenticated-orcid":false,"given":"David","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"LEAF\u2014Linking Engineering, Agriculture and Food, Departamento de Ci\u00eancias e Engenharia de Biossistemas, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6362-8255","authenticated-orcid":false,"given":"Hugo","family":"Os\u00f3rio","sequence":"additional","affiliation":[{"name":"i3S\u2014Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto, 4200-135 Porto, Portugal"},{"name":"Ipatimup\u2014Institute of Molecular Pathology and Immunology of the University of Porto, University of Porto, 4200-135 Porto, Portugal"},{"name":"Department of Pathology, Faculty of Medicine, University of Porto, 4200-319 Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4502-667X","authenticated-orcid":false,"given":"M\u00e1rio","family":"Pinho","sequence":"additional","affiliation":[{"name":"CIISA\u2014Centro de Investiga\u00e7\u00e3o Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterin\u00e1ria, Universidade de Lisboa, Avenida da Universidade T\u00e9cnica, Alto da Ajuda, 1300-477 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6755-4572","authenticated-orcid":false,"given":"Paula","family":"Lopes","sequence":"additional","affiliation":[{"name":"CIISA\u2014Centro de Investiga\u00e7\u00e3o Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterin\u00e1ria, Universidade de Lisboa, Avenida da Universidade T\u00e9cnica, Alto da Ajuda, 1300-477 Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1909-4540","authenticated-orcid":false,"given":"Jorge","family":"Correia","sequence":"additional","affiliation":[{"name":"CIISA\u2014Centro de Investiga\u00e7\u00e3o Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterin\u00e1ria, Universidade de Lisboa, Avenida da Universidade T\u00e9cnica, Alto da Ajuda, 1300-477 Lisboa, Portugal"}]},{"given":"Rui","family":"Pinto","sequence":"additional","affiliation":[{"name":"iMed UL, Faculdade de Farm\u00e1cia, Universidade de Lisboa, Av. Professor Gama Pinto, 1649-003 Lisboa, Portugal"},{"name":"JCS\u2014Laborat\u00f3rio de An\u00e1lises Cl\u00ednicas Dr. Joaquim Chaves, Av. General Norton de Matos, 1495-148 Miraflores, Portugal"}]},{"given":"Teresa","family":"Costa","sequence":"additional","affiliation":[{"name":"Indukern Portugal, Lda., Centro Empresarial Sintra Estoril II, Rua P\u00e9 de Mouro, Edif\u00edcio C, Apartado 53, Estrada de Albarraque, 2710-335 Sintra, Portugal"}]},{"given":"Etienne","family":"Corrent","sequence":"additional","affiliation":[{"name":"Ajinomoto Animal Nutrition Europe, 32 rue Guersant, 75017 Paris, France"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1059-2930","authenticated-orcid":false,"given":"Tristan","family":"Chalvon-Demersay","sequence":"additional","affiliation":[{"name":"Ajinomoto Animal Nutrition Europe, 32 rue Guersant, 75017 Paris, France"},{"name":"Ajinomoto Co., Inc., Tokyo 104-8315, Japan"}]}],"member":"1968","published-online":{"date-parts":[[2021,4,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/S0301-6226(97)00057-2","article-title":"Factors Influencing the Structure and Function of the Small Intestine in the Weaned Pig: A Review","volume":"51","author":"Pluske","year":"1997","journal-title":"Livest. 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