{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T04:22:19Z","timestamp":1771906939501,"version":"3.50.1"},"reference-count":64,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2021,7,9]],"date-time":"2021-07-09T00:00:00Z","timestamp":1625788800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Funda\u00e7\u00e3o para a Ciencia e Tecnologia","award":["UIDB\/00276\/2020"],"award-info":[{"award-number":["UIDB\/00276\/2020"]}]},{"name":"Funda\u00e7\u00e3o para a Ciencia e Tecnologia","award":["PTDC\/CVT-REP\/2863\/2012"],"award-info":[{"award-number":["PTDC\/CVT-REP\/2863\/2012"]}]},{"name":"Funda\u00e7\u00e3o para a Ciencia e Tecnologia","award":["UIDB\/04539\/2020"],"award-info":[{"award-number":["UIDB\/04539\/2020"]}]},{"name":"Funda\u00e7\u00e3o para a Ciencia e Tecnologia","award":["SFRH\/BD\/145531\/2019"],"award-info":[{"award-number":["SFRH\/BD\/145531\/2019"]}]},{"name":"Rural Development Programme","award":["PDR2020-101-03112"],"award-info":[{"award-number":["PDR2020-101-03112"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Oxidative stress and mitochondrial dysfunction have been associated with the age-related decline of oocyte quality and strategies for their prevention are currently quested. Urolithin A (UA) is a natural metabolite with pro-apoptotic and antioxidant effects, capable of preventing the accumulation of dysfunctional mitochondria in different aged cells. UA has never been tested in bovine oocytes. Our aim was to study the effect of UA on the developmental potential of cumulus-oocyte-complexes (COCs) and granulosa cells\u2019 (GCs) expression of important genes related to reproductive competence. Nuclear maturation progression, mitochondrial membrane potential (MMP) and developmental competence of physiologically mature (22 h) and in vitro aged oocytes (30 h of IVM) obtained from prepubertal and adult females, either supplemented with UA or not were assessed. Additionally, the amount of mRNA of several genes (NFE2L2, NQO1, and mt-DN5) and the number of mt-ND5 DNA copies were quantified in cultured GCs from prepubertal and adult females, either supplemented with UA or not. Our study confirmed the harmful effect of oocyte aging on the nuclear maturation progression, MMP, developmental competence and gene expression levels. UA treatment during in vitro maturation enhanced (p &lt; 0.05) the maturation rate and subsequent developmental capacity of aged oocytes. A positive effect (p &lt; 0.05) of UA on physiological maturation, MMP and embryonic development was also identified. UA also interfered on the expression profile of NFE2L2 and NQO1 genes in GCs cultures. Our findings demonstrate that UA supplementation is an effective way to prevent oocyte aging and improves the subsequent bovine embryonic development.<\/jats:p>","DOI":"10.3390\/ani11072048","type":"journal-article","created":{"date-parts":[[2021,7,9]],"date-time":"2021-07-09T10:50:38Z","timestamp":1625827838000},"page":"2048","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Anti-Aging Effect of Urolithin A on Bovine Oocytes In Vitro"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1654-143X","authenticated-orcid":false,"given":"\u00c9lisa","family":"Fonseca","sequence":"first","affiliation":[{"name":"Instituto Nacional de Investiga\u00e7\u00e3o Agr\u00e1ria e Veterin\u00e1ria, Quinta da Fonte Boa, 2005-048 Santarem, Portugal"}]},{"given":"Carla Cruz","family":"Marques","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Investiga\u00e7\u00e3o Agr\u00e1ria e Veterin\u00e1ria, Quinta da Fonte Boa, 2005-048 Santarem, Portugal"}]},{"given":"Jorge","family":"Pimenta","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Investiga\u00e7\u00e3o Agr\u00e1ria e Veterin\u00e1ria, Quinta da Fonte Boa, 2005-048 Santarem, Portugal"},{"name":"Centro de Investiga\u00e7\u00e3o Interdisciplinar em Sanidade Animal, Universidade de Lisboa, Av. da Universidade T\u00e9cnica, 1300-477 Lisboa, Portugal"}]},{"given":"Joana","family":"Jorge","sequence":"additional","affiliation":[{"name":"Group of Environment, Genetics and Oncobiology, Faculty of Medicine, Coimbra Institute for Clinical and Biomedical Research, University Coimbra, 3000-548 Coimbra, Portugal"},{"name":"Center for Innovative Biomedicine and Biotechnology, University Coimbra, 3004-504 Coimbra, Portugal"}]},{"given":"Maria Concei\u00e7\u00e3o","family":"Baptista","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Investiga\u00e7\u00e3o Agr\u00e1ria e Veterin\u00e1ria, Quinta da Fonte Boa, 2005-048 Santarem, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1470-4802","authenticated-orcid":false,"given":"Ana Cristina","family":"Gon\u00e7alves","sequence":"additional","affiliation":[{"name":"Group of Environment, Genetics and Oncobiology, Faculty of Medicine, Coimbra Institute for Clinical and Biomedical Research, University Coimbra, 3000-548 Coimbra, Portugal"},{"name":"Center for Innovative Biomedicine and Biotechnology, University Coimbra, 3004-504 Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7514-6130","authenticated-orcid":false,"given":"Rosa M. L. N.","family":"Pereira","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Investiga\u00e7\u00e3o Agr\u00e1ria e Veterin\u00e1ria, Quinta da Fonte Boa, 2005-048 Santarem, Portugal"},{"name":"Centro de Investiga\u00e7\u00e3o Interdisciplinar em Sanidade Animal, Universidade de Lisboa, Av. da Universidade T\u00e9cnica, 1300-477 Lisboa, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2021,7,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1530\/REP-07-0021","article-title":"Oocyte Developmental Competence in a Bovine Model of Reproductive Aging","volume":"134","author":"Malhi","year":"2007","journal-title":"Reproduction"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"96588","DOI":"10.18632\/oncotarget.21731","article-title":"Systemic Analysis of Gene Expression Profiles in Porcine Granulosa Cells during Aging","volume":"8","author":"Hui","year":"2017","journal-title":"Oncotarget"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"881","DOI":"10.1530\/REP-17-0134","article-title":"In Vitro Maturation Impacts Cumulus\u2013Oocyte Complex Metabolism and Stress in Cattle","volume":"154","author":"Oliveira","year":"2017","journal-title":"Reproduction"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1071\/RD12295","article-title":"Reductions in the Number of Mid-Sized Antral Follicles Are Associated with Markers of Premature Ovarian Senescence in Dairy Cows","volume":"26","author":"Modina","year":"2014","journal-title":"Reprod. 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