{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T13:44:24Z","timestamp":1762868664461,"version":"build-2065373602"},"reference-count":87,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2021,10,21]],"date-time":"2021-10-21T00:00:00Z","timestamp":1634774400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["POCI-01-0145-FEDER-029947, UIDB\/04033\/2020, UIDB\/CVT\/0772\/2020"],"award-info":[{"award-number":["POCI-01-0145-FEDER-029947, UIDB\/04033\/2020, UIDB\/CVT\/0772\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Animals"],"abstract":"<jats:p>Wasting disease in small ruminants is frequently detected at slaughterhouses. The wasting disorder is manifested by the deterioration of the nutritional and physiological state of the animal indicated by thinness, emaciation, and cachexia. Evidence of emaciation and cachexia, alone, are pathological conditions leading to carcass condemnation during an inspection. Several diseases are associated with a wasting condition, including scrapie, pseudotuberculosis, tuberculosis, paratuberculosis, Maedi Visna, and tumor diseases. On the other hand, parasitic diseases, nutrition disorders, exposure or ingestion of toxins, metabolic conditions, inadequate nutrition due to poor teeth, or poor alimentary diet are conditions contributing to poor body condition. Classical and atypical scrapie is naturally occurring transmissible spongiform encephalopathies in small ruminants. The etiological agent for each one is prions. However, each of these scrapie types is epidemiologically, pathologically, and biochemically different. Though atypical scrapie occurs at low incidence, it is consistently prevalent in the small ruminant population. Hence, it is advisable to include differential diagnosis of this disease, from other possibilities, as a cause of wasting conditions detected during meat inspection at the abattoir. This manuscript is a review of the measures in force at the abattoir for scrapie control, focusing on the differential diagnosis of gross lesions related to wasting conditions detected in small ruminants during meat inspection.<\/jats:p>","DOI":"10.3390\/ani11113028","type":"journal-article","created":{"date-parts":[[2021,10,21]],"date-time":"2021-10-21T14:37:48Z","timestamp":1634827068000},"page":"3028","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Scrapie at Abattoir: Monitoring, Control, and Differential Diagnosis of Wasting Conditions during Meat Inspection"],"prefix":"10.3390","volume":"11","author":[{"given":"Alexandra","family":"Esteves","sequence":"first","affiliation":[{"name":"Animal and Veterinary Research Centre (CECAV), University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8588-7071","authenticated-orcid":false,"given":"Madalena","family":"Vieira-Pinto","sequence":"additional","affiliation":[{"name":"Animal and Veterinary Research Centre (CECAV), University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6934-1669","authenticated-orcid":false,"given":"H\u00e9lder","family":"Quintas","sequence":"additional","affiliation":[{"name":"Centro de Investiga\u00e7\u00e3o de Montanha (CIMO), Instituto Polit\u00e9cnico de Bragan\u00e7a, Campus de Santa Apol\u00f3nia, 5300-253 Bragan\u00e7a, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2673-2758","authenticated-orcid":false,"given":"Leonor","family":"Orge","sequence":"additional","affiliation":[{"name":"Animal and Veterinary Research Centre (CECAV), University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"},{"name":"Pathology Laboratory, UEISPSA, National Institute for Agricultural and Veterinary Research (INIAV), I.P., 2780-157 Oeiras, Portugal"}]},{"given":"Adelina","family":"Gama","sequence":"additional","affiliation":[{"name":"Animal and Veterinary Research Centre (CECAV), University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"}]},{"given":"Anabela","family":"Alves","sequence":"additional","affiliation":[{"name":"Animal and Veterinary Research Centre (CECAV), University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1912-1457","authenticated-orcid":false,"given":"Fernanda","family":"Seixas","sequence":"additional","affiliation":[{"name":"Animal and Veterinary Research Centre (CECAV), University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6330-4560","authenticated-orcid":false,"given":"Isabel","family":"Pires","sequence":"additional","affiliation":[{"name":"Animal and Veterinary Research Centre (CECAV), University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5928-6483","authenticated-orcid":false,"given":"Maria de Lurdes","family":"Pinto","sequence":"additional","affiliation":[{"name":"Animal and Veterinary Research Centre (CECAV), University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9610-5760","authenticated-orcid":false,"given":"Ana Paula","family":"Mendon\u00e7a","sequence":"additional","affiliation":[{"name":"Pathology Laboratory, UEISPSA, National Institute for Agricultural and Veterinary Research (INIAV), I.P., 2780-157 Oeiras, Portugal"}]},{"given":"Carla","family":"Lima","sequence":"additional","affiliation":[{"name":"Pathology Laboratory, UEISPSA, National Institute for Agricultural and Veterinary Research (INIAV), I.P., 4485-655 Vila do Conde, Portugal"}]},{"given":"Carla Neves","family":"Machado","sequence":"additional","affiliation":[{"name":"Pathology Laboratory, UEISPSA, National Institute for Agricultural and Veterinary Research (INIAV), I.P., 2780-157 Oeiras, Portugal"}]},{"given":"Jo\u00e3o Carlos","family":"Silva","sequence":"additional","affiliation":[{"name":"Pathology Laboratory, UEISPSA, National Institute for Agricultural and Veterinary Research (INIAV), I.P., 2780-157 Oeiras, Portugal"}]},{"given":"Paula","family":"Tavares","sequence":"additional","affiliation":[{"name":"Pathology Laboratory, UEISPSA, National Institute for Agricultural and Veterinary Research (INIAV), I.P., 4485-655 Vila do Conde, Portugal"}]},{"given":"Filipe","family":"Silva","sequence":"additional","affiliation":[{"name":"Animal and Veterinary Research Centre (CECAV), University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"}]},{"given":"Estela","family":"Bastos","sequence":"additional","affiliation":[{"name":"Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"},{"name":"Genetic Department, University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1472-1613","authenticated-orcid":false,"given":"Jorge","family":"Pereira","sequence":"additional","affiliation":[{"name":"Animal and Veterinary Research Centre (CECAV), University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0586-2550","authenticated-orcid":false,"given":"Nuno","family":"Gon\u00e7alves-Anjo","sequence":"additional","affiliation":[{"name":"Genetic Department, University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7832-6292","authenticated-orcid":false,"given":"Paulo","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Pathology Laboratory, UEISPSA, National Institute for Agricultural and Veterinary Research (INIAV), I.P., 2780-157 Oeiras, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3191-7853","authenticated-orcid":false,"given":"Roberto","family":"Sargo","sequence":"additional","affiliation":[{"name":"Animal and Veterinary Research Centre (CECAV), University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"}]},{"given":"Ana","family":"Matos","sequence":"additional","affiliation":[{"name":"Research Center for Natural Resources, Environment and Society (CERNAS), Polytechnic Institute of Castelo Branco (IPCB), 6000-767 Castelo Branco, Portugal"},{"name":"Quality of Life in the Rural World (Q-Rural), Polytechnic Institute of Castelo Branco (IPCB), 6000-767 Castelo Branco, Portugal"}]},{"given":"Lu\u00eds","family":"Figueira","sequence":"additional","affiliation":[{"name":"Quality of Life in the Rural World (Q-Rural), Polytechnic Institute of Castelo Branco (IPCB), 6000-767 Castelo Branco, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9473-5283","authenticated-orcid":false,"given":"Maria dos Anjos","family":"Pires","sequence":"additional","affiliation":[{"name":"Animal and Veterinary Research Centre (CECAV), University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal"}]}],"member":"1968","published-online":{"date-parts":[[2021,10,21]]},"reference":[{"key":"ref_1","unstructured":"Official Journal of European Union (2021, October 15). Commission Implementing Regulation (EU) 2019\/627 of 15 March 2019 Laying down Uniform Practical Arrangements for the Performance of Official Controls on Products of Animal Origin Intended for Human Consumption in Accordance with Regulation (EU) 2017\/625 of the European Parliament and of the Council and Amending Commission Regulation (EC) No 2074\/2005 as Regards Official Controls (Text with EEA Relevance). Available online: https:\/\/eur-lex.europa.eu\/legal-content\/EN\/TXT\/PDF\/?uri=OJ:L:2019:131:FULL."},{"key":"ref_2","unstructured":"Jensen, H.E., Leifsson, P.S., Nielsen, O.L., Agerholm, J.S., and Iburg, T. (2017). Meat Inspection: The Pathoanatomic Basis, Biofolia. [1st ed.]."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Ninios, T., Lund\u00e9n, J., Korkeala, H., and Fredriksson-Ahomaa, M. (2014). Meat Inspection Lesions. Meat Inspection and Control in the Slaughterhouse, John Wiley & Sons Inc.","DOI":"10.1002\/9781118525821"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Collins, D.S., Huey, R.J., and Gracey, J.F. (2015). Meat Inspection Protocols. Gracey\u2019s Meat Hygiene, John Wiley & Sons Inc.","DOI":"10.1002\/9781118649985"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1177\/0300985818794247","article-title":"Review: Update on Classical and Atypical Scrapie in Sheep and Goats","volume":"56","author":"Greenlee","year":"2019","journal-title":"Vet. Pathol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1111\/bpa.12696","article-title":"Animal Prion Diseases: The Risks to Human Health: Animal Prion Diseases","volume":"29","author":"Houston","year":"2019","journal-title":"Brain Pathol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"33","DOI":"10.2527\/af.2014-0005","article-title":"Classical and Atypical TSE in Small Ruminants","volume":"4","author":"Beringue","year":"2014","journal-title":"Anim. Front."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1051\/vetres:2007056","article-title":"Atypical\/Nor98 Scrapie: Properties of the Agent, Genetics, and Epidemiology","volume":"39","author":"Benestad","year":"2008","journal-title":"Vet. Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1136\/vr.153.7.202","article-title":"Cases of Scrapie with Unusual Features in Norway and Designation of a New Type, Nor98","volume":"153","author":"Benestad","year":"2003","journal-title":"Vet. Record."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"3165","DOI":"10.1099\/jgv.0.000234","article-title":"Archival Search for Historical Atypical Scrapie in Sheep Reveals Evidence for Mixed Infections","volume":"96","author":"Chong","year":"2015","journal-title":"J. Gen. Virol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"863","DOI":"10.1177\/104063871002200604","article-title":"Atypical Scrapie\/Nor98 in a Sheep from New Zealand","volume":"22","author":"Kittelberger","year":"2010","journal-title":"J. Vet. Diagn. Investig."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1111\/avj.12529","article-title":"Atypical Scrapie in Australia","volume":"94","author":"Cook","year":"2016","journal-title":"Aust. Vet. J."},{"key":"ref_13","unstructured":"OIE (2021, August 04). Scrapie, Chapter 3.8.11. (Version Adopted in May 2016) in Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2021. Available online: https:\/\/www.oie.int\/en\/what-we-do\/standards\/codes-and-manuals\/terrestrial-manual-online-access\/."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"11573","DOI":"10.1038\/srep11573","article-title":"Transmission of Scrapie Prions to Primate after an Extended Silent Incubation Period","volume":"5","author":"Comoy","year":"2015","journal-title":"Sci. Rep."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"5821","DOI":"10.1038\/ncomms6821","article-title":"Evidence for Zoonotic Potential of Ovine Scrapie Prions","volume":"5","author":"Cassard","year":"2014","journal-title":"Nat. Commun."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"26853","DOI":"10.1073\/pnas.1915737116","article-title":"The Emergence of Classical BSE from Atypical\/Nor98 Scrapie","volume":"116","author":"Huor","year":"2019","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1146\/annurev-genet-120213-092352","article-title":"Genetic Factors in Mammalian Prion Diseases","volume":"53","author":"Mead","year":"2019","journal-title":"Annu. Rev. Genet."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.virusres.2014.11.026","article-title":"Prion and prion-like diseases in animals","volume":"207","author":"Espinosa","year":"2015","journal-title":"Virus Res"},{"key":"ref_19","first-page":"e51101","article-title":"Clinical Examination Protocol to Detect Atypical and Classical Scrapie in Sheep","volume":"83","author":"Konold","year":"2014","journal-title":"J. Vis. Exp."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1007\/s00401-008-0433-8","article-title":"Neuroanatomical distribution of abnormal prion protein in naturally occurring atypical scrapie cases in Great Britain","volume":"116","author":"Moore","year":"2008","journal-title":"Acta Neuropathol."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"EFSA Panel on Biological Hazards (BIOHAZ) (2014). Scientific Opinion on the Scrapie Situation in the EU after 10 Years of Monitoring and Control in Sheep and Goats. EFSA J., 12, 3781.","DOI":"10.2903\/j.efsa.2014.3781"},{"key":"ref_22","unstructured":"(2021, August 06). Regulation (EC) No. 999\/2001 Laying down Rules for the Prevention, Control and Eradication of Certain Transmissible Spongiform Encephalopathies. Available online: https:\/\/www.ecolex.org\/details\/legislation\/regulation-ec-no-9992001-laying-down-rules-for-the-prevention-control-and-eradication-of-certain-transmissible-spongiform-encephalopathies-lex-faoc030438\/."},{"key":"ref_23","unstructured":"(2021, August 30). EUR-Lex\u201432016R0429-EN-EUR-Lex. Available online: https:\/\/eur-lex.europa.eu\/eli\/reg\/2016\/429\/oj."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"European Food Safety Authority (EFSA) (2020). The European Union Summary Report on Surveillance for the Presence of Transmissible Spongiform Encephalopathies (TSE) in 2019. EFS2, 18.","DOI":"10.2903\/j.efsa.2020.6303"},{"key":"ref_25","unstructured":"(2021, August 06). EUR-Lex\u201432013R0630-EN-EUR-Lex. Available online: https:\/\/eur-lex.europa.eu\/legal-content\/GA\/TXT\/?uri=CELEX%3A32013R0630."},{"key":"ref_26","unstructured":"Official Journal of European Union (2021, October 15). Commission Regulation (EU) 2018\/969 of 9 July 2018 Amending Annex V to Regulation (EC) No 999\/2001 of the European Parliament and of the Council as Regards the Requirements for the Removal of Specified Risk Materials from Small Ruminants (Text with EEA Relevance). Available online: https:\/\/eur-lex.europa.eu\/legal-content\/EN\/TXT\/PDF\/?uri=CELEX:32018R0969&from=FR."},{"key":"ref_27","unstructured":"(2021, August 04). Regulation (EC) No. 1069\/2009 of the European Parliament and of the Council Laying down Health Rules as Regards Animal by-Products and Derived Products Not Intended for Human Consumption and Repealing Regulation (EC) No. 1774\/2002 (Animal by-Products Regulation). Available online: https:\/\/www.ecolex.org\/details\/legislation\/regulation-ec-no-10692009-of-the-european-parliament-and-of-the-council-laying-down-health-rules-as-regards-animal-by-products-and-derived-products-not-intended-for-human-consumption-and-repealing-regulation-ec-no-17742002-animal-by-products-regulation-lex-faoc091925\/."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/j.smallrumres.2010.07.011","article-title":"Abattoir Survey on Caseous Lymphadenitis in Sheep and Goats in Tanta, Egypt","volume":"94","author":"Osman","year":"2010","journal-title":"Small Rumin. Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/j.jcpa.2007.07.002","article-title":"Corynebacterium Pseudotuberculosis and Its Role in Ovine Caseous Lymphadenitis","volume":"137","author":"Baird","year":"2007","journal-title":"J. Comp. Pathol."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Ruiz, H., Ferrer, L.M., Ramos, J.J., Baselga, C., Alzuguren, O., Tejedor, M.T., de Miguel, R., and Lacasta, D. (2020). The Relevance of Caseous Lymphadenitis as a Cause of Culling in Adult Sheep. Animals, 10.","DOI":"10.3390\/ani10111962"},{"key":"ref_31","first-page":"45","article-title":"Aspectos Epidemiologicos y Clinicos de La Tuberculosis Caprina","volume":"46","author":"Garcia","year":"1996","journal-title":"Ovis Tratado Patol. Prod. Ovina"},{"key":"ref_32","first-page":"61","article-title":"Diagnostico de La Tuberculosis Caprina","volume":"46","author":"Garcia","year":"1996","journal-title":"Ovis Tratado Patol. Prod. Ovina"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.vetmic.2015.08.007","article-title":"Small Ruminant Lentivirus Infections and Diseases","volume":"181","author":"Reina","year":"2015","journal-title":"Vet. Microbiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1186\/s13567-015-0203-2","article-title":"Genetic Diversity of Mycobacterium Avium Subspecies Paratuberculosis and the Influence of Strain Type on Infection and Pathogenesis: A Review","volume":"46","author":"Stevenson","year":"2015","journal-title":"Vet. Res."},{"key":"ref_35","first-page":"999","article-title":"Caseous Lymphadenitis in Goats","volume":"25","author":"Ivanovic","year":"2009","journal-title":"Biotechnol. Anim."},{"key":"ref_36","unstructured":"Chapman, C., and Kennedy, M. (2017). Caseous Lymphadenitis Management in Goats, All Current Publications. Available online: https:\/\/digitalcommons.usu.edu\/extension_curall\/1647."},{"key":"ref_37","first-page":"7","article-title":"The Epidemiology and Pathophysiology of Caseous Lymphadenitis: A Review","volume":"5","author":"Osman","year":"2018","journal-title":"J. Vet. Med. Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2020\/8822997","article-title":"Survival Analysis of Clinical Cases of Caseous Lymphadenitis of Goats in North Shoa, Ethiopia","volume":"2020","author":"Yitagesu","year":"2020","journal-title":"Vet. Med. Int."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1016\/j.smallrumres.2014.11.013","article-title":"Diagnosis of Caseous Lymphadenitis in Sheep and Goat","volume":"123","author":"Oreiby","year":"2015","journal-title":"Small Rumin. Res."},{"key":"ref_40","unstructured":"Bernab\u00e9, A., Navarro, J.A., G\u00f3mez, S., S\u00e1nchez, J., Sidrach, J., Menchen, V., Vera, A., and Sierra, M.A. (1990). Morphopathology of Caprine Tuberculosis. I. Pulmonary Tuberculosis. An. Vet. Murcia, 6\u201320."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/j.tvjl.2011.05.006","article-title":"Tuberculosis Due to Mycobacterium Bovis and Mycobacterium Caprae in Sheep","volume":"191","author":"Ocampo","year":"2012","journal-title":"Vet. J."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1136\/vr.c1678","article-title":"Tuberculosis in Goats","volume":"166","author":"Quintas","year":"2010","journal-title":"Vet. Rec."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.jcpa.2010.12.006","article-title":"Microscopical and Immunological Features of Tuberculoid Granulomata and Cavitary Pulmonary Tuberculosis in Naturally Infected Goats","volume":"145","author":"Sanchez","year":"2011","journal-title":"J. Comp. Pathol."},{"key":"ref_44","unstructured":"Pugh, D.G., and Baird, A.N. (2012). Diseases of the Respiratory System. Sheep and Goat Medicine, Elsevier\/Saunders. Veterinary Medicine."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1107","DOI":"10.1016\/j.rvsc.2013.10.003","article-title":"Ante-Mortem Diagnosis of Caprine Tuberculosis in Persistently Infected Herds: Influence of Lesion Type on the Sensitivity of Diagnostic Tests","volume":"95","author":"Navarro","year":"2013","journal-title":"Res. Vet. Sci."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"606","DOI":"10.1111\/rda.12332","article-title":"Coexistence of Tuberculosis and Mammary Carcinoma in a Goat","volume":"49","author":"Quintas","year":"2014","journal-title":"Reprod. Dom. Anim."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1007\/s11250-017-1252-5","article-title":"An Assessment of Caprine Tuberculosis Prevalence in Lubumbashi Slaughterhouse, Democratic Republic of Congo","volume":"49","author":"Luboya","year":"2017","journal-title":"Trop. Anim. Health Prod."},{"key":"ref_48","unstructured":"Sim\u00f5es, J., and Guti\u00e9rrez, C. (2017). Diagnosis of Mycobacteriosis in Goats: Tuberculosis and Paratuberculosis. Sustainable Goat Production in Adverse Environments: Volume I, Springer International Publishing."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Matthews, J.G. (2016). Chronic weight loss. Diseases of the Goat, John Wiley & Sons Inc.","DOI":"10.1002\/9781119073543.ch9"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Hodgeman, R., Mann, R., Savin, K., Djitro, N., Rochfort, S., and Rodoni, B. (2021). Molecular Characterisation of Mycobacterium Avium Subsp. Paratuberculosis in Australia. BMC Microbiol., 21.","DOI":"10.1186\/s12866-021-02140-2"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"572724","DOI":"10.3389\/fvets.2020.572724","article-title":"Mycobacterium Avium Subspecies Paratuberculosis Infection in Zoo Animals: A Review of Susceptibility and Disease Process","volume":"7","author":"Roller","year":"2020","journal-title":"Front. Vet. Sci."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1591","DOI":"10.1128\/jcm.28.7.1591-1596.1990","article-title":"Identification of Two Groups of Mycobacterium Paratuberculosis Strains by Restriction Endonuclease Analysis and DNA Hybridization","volume":"28","author":"Collins","year":"1990","journal-title":"J. Clin. Microbiol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1798","DOI":"10.1128\/JCM.40.5.1798-1804.2002","article-title":"Molecular Characterization of Pigmented and Nonpigmented Isolates of Mycobacterium Avium Subsp. Paratuberculosis","volume":"40","author":"Stevenson","year":"2002","journal-title":"J. Clin. Microbiol."},{"key":"ref_54","unstructured":"OIE Paratuberculosis, Chapter 3.8.11. Terrestrial Animal Health Code, OIE. Available online: https:\/\/www.cfsph.iastate.edu\/Factsheets\/pdfs\/paratuberculosis.pdf."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Kalogianni, A.I., Bossis, I., Ekateriniadou, L.V., and Gelasakis, A.I. (2020). Etiology, Epizootiology and Control of Maedi-Visna in Dairy Sheep: A Review. Animals, 10.","DOI":"10.3390\/ani10040616"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.jcpa.2018.01.001","article-title":"Inflammatory Lesion Patterns in Target Organs of Visna\/Maedi in Sheep and Their Significance in the Pathogenesis and Diagnosis of the Infection","volume":"159","author":"Gayo","year":"2018","journal-title":"J. Comp. Pathol."},{"key":"ref_57","unstructured":"Maxie, M.G. (2016). Respiratory System. Jubb, Kennedy, and Palmer\u2019s Pathology of Domestic Animals, Elsevier."},{"key":"ref_58","unstructured":"Zachary, J.F. (2017). Alimentary System and the Peritoneum, Omentum, Mesentery, and Peritoneal Cavity. Pathologic Basis of Veterinary Disease, Elsevier."},{"key":"ref_59","first-page":"1","article-title":"Some Pathological Features of Lungs from Domestic and Wild Ruminants with Single and Mixed Protostrongylid Infections","volume":"2010","author":"Alexandrov","year":"2010","journal-title":"Vet. Med. Int."},{"key":"ref_60","unstructured":"Pugh, D.G., and Baird, A.N. (2012). Internal Parasites. Sheep and Goat Medicine, Elsevier\/Saunders. Veterinary Medicine."},{"key":"ref_61","unstructured":"Zachary, J.F. (2017). Respiratory System, Mediastinum, and Pleurae. Pathologic Basis of Veterinary Disease, Elsevier."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/j.smallrumres.2011.10.022","article-title":"Coccidiosis Due to Eimeria in Sheep and Goats, a Review","volume":"103","author":"Chartier","year":"2012","journal-title":"Small Rumin. Res."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1051\/parasite\/2004113293","article-title":"The Effects of Concurrent Experimental Infections of Sheep with Trichostrongylus Colubriformis and T. Vitrinus on Nematode Distributions, Numbers and on Pathological Changes","volume":"11","author":"Roy","year":"2004","journal-title":"Parasite"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/0169-4758(89)90269-X","article-title":"The Veterinary Importance of Nodular Worms (Olesophagostomum spp)","volume":"5","author":"Stewart","year":"1989","journal-title":"Parasitol. Today"},{"key":"ref_65","first-page":"1","article-title":"Alimentary System","volume":"Volume 2","author":"Maxie","year":"2016","journal-title":"Jubb, Kennedy, and Palmer\u2019s Pathology of Domestic Animals"},{"key":"ref_66","first-page":"9","article-title":"An Overview of Intestinal Coccidiosis in Sheep and Goats","volume":"167","author":"Hashemnia","year":"2017","journal-title":"Rev. Med. Vet."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.vetpar.2012.03.029","article-title":"Update on Trematode Infections in Sheep","volume":"189","author":"Meana","year":"2012","journal-title":"Vet. Parasitol."},{"key":"ref_68","first-page":"465","article-title":"Liver and Biliary System","volume":"Volume 2","author":"Maxie","year":"2016","journal-title":"Jubb, Kennedy, and Palmer\u2019s Pathology of Domestic Animals"},{"key":"ref_69","unstructured":"Zachary, J.F. (2017). Hepatobiliary System and Exocrine Pancreas. Pathologic Basis of Veterinary Disease, Elsevier."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"668","DOI":"10.1177\/0300985812471544","article-title":"One Hundred Two Tumors in 100 Goats (1987\u20132011)","volume":"50","year":"2013","journal-title":"Vet. Pathol."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1136\/inpract.12.2.68","article-title":"Tumours in Sheep","volume":"12","author":"Head","year":"1990","journal-title":"Practice"},{"key":"ref_72","doi-asserted-by":"crossref","unstructured":"Hindson, J.C., and Winter, A.C. (2002). Manual of Sheep Diseases, Blackwell Science. [2nd ed.].","DOI":"10.1002\/9780470752449"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.smallrumres.2019.05.014","article-title":"Ovine Pulmonary Adenocarcinoma: A Transmissible Lung Cancer of Sheep, Difficult to Control","volume":"176","author":"Borobia","year":"2019","journal-title":"Small Rumin. Res."},{"key":"ref_74","unstructured":"(2021, August 04). OIE Ovine Pulmonary Adecocarcinoma (Adenomatosis), Terrestrial Manual Chapter 3.7.8. Available online: https:\/\/www.oie.int\/fileadmin\/Home\/eng\/Health_standards\/tahm\/3.07.08_OPA.pdf."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1007\/978-3-642-55638-8_2","article-title":"Pathology of Ovine Pulmonary Adenocarcinoma","volume":"Volume 275","author":"Fan","year":"2003","journal-title":"Jaagsiekte Sheep Retrovirus and Lung Cancer"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"6964","DOI":"10.1128\/JVI.73.8.6964-6972.1999","article-title":"Jaagsiekte Sheep Retrovirus Is Necessary and Sufficient To Induce a Contagious Lung Cancer in Sheep","volume":"73","author":"Palmarini","year":"1999","journal-title":"J. Virol."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1099\/vir.0.19547-0","article-title":"Endogenous Betaretroviruses of Sheep: Teaching New Lessons in Retroviral Interference and Adaptation","volume":"85","author":"Palmarini","year":"2004","journal-title":"J. Gen. Virol."},{"key":"ref_78","doi-asserted-by":"crossref","unstructured":"As\u00edn, J., Ram\u00edrez, G.A., Navarro, M.A., Nyaoke, A.C., Henderson, E.E., Mendon\u00e7a, F.S., Mol\u00edn, J., and Uzal, F.A. (2021). Nutritional Wasting Disorders in Sheep. Animals, 11.","DOI":"10.3390\/ani11020501"},{"key":"ref_79","first-page":"571","article-title":"Unexplained Weight Loss in Sheep and Goats: A Guide to Differential Diagnosis, Therapy, and Management","volume":"5","author":"Sherman","year":"1983","journal-title":"Vet. Clin. N. Am. Large Anim. Pract."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1007\/s12011-018-1578-5","article-title":"Copper and Zinc Nutritional Issues for Agricultural Animal Production","volume":"188","author":"Hill","year":"2019","journal-title":"Biol. Trace Elem. Res."},{"key":"ref_81","first-page":"270","article-title":"Cobalt-Vitamin B12 Deficiency Causes Lipid Accumulation, Lipid Peroxidation and Decreased Alpha-Tocopherol Concentrations in the Liver of Sheep","volume":"64","author":"Kennedy","year":"1994","journal-title":"Int. J. Vitam. Nutr. Res."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1023\/A:1006244925482","article-title":"Hepatic Lipidosis Associated with Cobalt Deficiency in Omani Goats","volume":"23","author":"Johnson","year":"1999","journal-title":"Vet. Res. Commun."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1177\/030098589703400605","article-title":"Histopathologic and Ultrastructural Alterations of White Liver Disease in Sheep Experimentally Depleted of Cobalt","volume":"34","author":"Kennedy","year":"1997","journal-title":"Vet. Pathol."},{"key":"ref_84","unstructured":"(2021, August 05). EUR-Lex\u201432001R0999-EN-EUR-Lex. Available online: https:\/\/eur-lex.europa.eu\/legal-content\/EN\/ALL\/?uri=CELEX%3A32001R0999."},{"key":"ref_85","unstructured":"(2021, August 05). EUR-Lex\u201402001R0999-20201119-EN-EUR-Lex. Available online: https:\/\/eur-lex.europa.eu\/legal-content\/EN\/TXT\/?uri=CELEX%3A02001R0999-20201119."},{"key":"ref_86","unstructured":"Codex Alimentarius (1994). Carne y Productos C\u00e1rnicos Incluso Los \u201cBouillons\u201d y Consom\u00e9s. Volumen 10, Programa Conjunto FAO\/OMS Sobre Normas Alimentarias, Comision Del Codex Alimentarius."},{"key":"ref_87","unstructured":"Gracey, J., Collins, D.S., and Motzer, R. (1999). Pathology, in Meat Hygiene, W.B. Saunders Company. [10th ed.]."}],"container-title":["Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2615\/11\/11\/3028\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T07:20:31Z","timestamp":1760167231000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2615\/11\/11\/3028"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,10,21]]},"references-count":87,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2021,11]]}},"alternative-id":["ani11113028"],"URL":"https:\/\/doi.org\/10.3390\/ani11113028","relation":{},"ISSN":["2076-2615"],"issn-type":[{"type":"electronic","value":"2076-2615"}],"subject":[],"published":{"date-parts":[[2021,10,21]]}}}